BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0212.5
(129 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC16653 similar to UP|Q8W1A1 (Q8W1A1) Adenosine 5'-phosphosulfat... 108 3e-26
AV409576 32 0.034
TC7823 similar to UP|O24470 (O24470) Lipoxygenase , partial (96%) 27 1.4
AV780688 26 2.5
TC11418 similar to GB|AAP12872.1|30017277|BT006223 At3g57480 {Ar... 25 3.2
AV413102 25 4.2
TC18419 25 5.5
TC19986 24 7.1
TC17142 homologue to UP|Q9SPB9 (Q9SPB9) Ubiquitin carrier protei... 24 7.1
TC16303 weakly similar to UP|AGA1_YEAST (P32323) A-agglutinin at... 24 9.3
TC10982 weakly similar to UP|Q9AWB6 (Q9AWB6) Phospholipase PLDb2... 24 9.3
TC14350 similar to UP|PSAL_SPIOL (Q41385) Photosystem I reaction... 24 9.3
>TC16653 similar to UP|Q8W1A1 (Q8W1A1) Adenosine 5'-phosphosulfate
reductase, partial (69%)
Length = 1242
Score = 108 bits (270), Expect(2) = 3e-26
Identities = 69/143 (48%), Positives = 78/143 (54%), Gaps = 27/143 (18%)
Frame = +1
Query: 1 CCRVRKVRLLRRELKGVTT*IIGQ*KDQSPGT---------------------NLVKWNL 39
CCRVRKVR LRR LKG+ I GQ KDQSPGT +LVKWN
Sbjct: 625 CCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEVPVVQVDPVFEGVDGGVGSLVKWNP 804
Query: 40 VAISKAIIYGISLGP*MCL*IH*IRKDTF------PLEALVLREQHEREGNRWWEDAKAK 93
VA K L M + ++ + + P VL QHEREG WWEDAKAK
Sbjct: 805 VANVKGHDIWSFLRT-MNVPVNSLHSKGYISIGCEPCTRAVLPGQHEREGRWWWEDAKAK 981
Query: 94 DCGLHKGNVKQDVGAQLNGNGAA 116
+CGLHKGNVKQD A+LNGNG A
Sbjct: 982 ECGLHKGNVKQDAEAELNGNGVA 1050
Score = 24.3 bits (51), Expect(2) = 3e-26
Identities = 7/10 (70%), Positives = 9/10 (90%)
Frame = +3
Query: 120 HCHCRIHLKY 129
HCHCR HL++
Sbjct: 1062 HCHCRRHLQH 1091
>AV409576
Length = 418
Score = 32.0 bits (71), Expect = 0.034
Identities = 14/21 (66%), Positives = 16/21 (75%)
Frame = +1
Query: 1 CCRVRKVRLLRRELKGVTT*I 21
CCR RKVR LRR LKG+ *+
Sbjct: 355 CCRTRKVRPLRRALKGLRA*V 417
>TC7823 similar to UP|O24470 (O24470) Lipoxygenase , partial (96%)
Length = 2909
Score = 26.6 bits (57), Expect = 1.4
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = +1
Query: 80 EREGNRWWEDAKAKDCGLHKGNVKQDVGAQLNG 112
ERE W D KAK G+ ++ A++NG
Sbjct: 2413 ERENPEWTSDTKAKQAFQKFGSKLAEIEAKING 2511
>AV780688
Length = 459
Score = 25.8 bits (55), Expect = 2.5
Identities = 7/13 (53%), Positives = 10/13 (76%)
Frame = +2
Query: 77 EQHEREGNRWWED 89
E H+REG WW++
Sbjct: 416 ESHQREGRSWWKE 454
>TC11418 similar to GB|AAP12872.1|30017277|BT006223 At3g57480 {Arabidopsis
thaliana;}, partial (81%)
Length = 994
Score = 25.4 bits (54), Expect = 3.2
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = -1
Query: 114 GAAMQVHCHCRIHL 127
G A++VHCHC + L
Sbjct: 751 GIALRVHCHCSVPL 710
>AV413102
Length = 374
Score = 25.0 bits (53), Expect = 4.2
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +1
Query: 99 KGNVKQDVGAQLNGNG 114
+GN++QD A+ NGNG
Sbjct: 1 QGNLRQDGDAEFNGNG 48
>TC18419
Length = 409
Score = 24.6 bits (52), Expect = 5.5
Identities = 7/11 (63%), Positives = 10/11 (90%)
Frame = -1
Query: 77 EQHEREGNRWW 87
++ +REGNRWW
Sbjct: 184 KKRKREGNRWW 152
>TC19986
Length = 531
Score = 24.3 bits (51), Expect = 7.1
Identities = 8/18 (44%), Positives = 14/18 (77%)
Frame = +2
Query: 32 TNLVKWNLVAISKAIIYG 49
++L+KWNL+ IS + + G
Sbjct: 248 SHLIKWNLIRISPSTLQG 301
>TC17142 homologue to UP|Q9SPB9 (Q9SPB9) Ubiquitin carrier protein 4
(Ubiquitin-conjugating enzyme E2) (Ubiquitin-protein
ligase) , partial (77%)
Length = 534
Score = 24.3 bits (51), Expect = 7.1
Identities = 10/29 (34%), Positives = 14/29 (47%)
Frame = -2
Query: 68 FPLEALVLREQHEREGNRWWEDAKAKDCG 96
F +A ER+ + WED K + CG
Sbjct: 107 FFCDAFERERDRERDREKVWEDGKQEVCG 21
>TC16303 weakly similar to UP|AGA1_YEAST (P32323) A-agglutinin attachment
subunit precursor, partial (3%)
Length = 1235
Score = 23.9 bits (50), Expect = 9.3
Identities = 7/13 (53%), Positives = 10/13 (76%)
Frame = +3
Query: 76 REQHEREGNRWWE 88
+E+ +EG RWWE
Sbjct: 300 QEEAHQEGRRWWE 338
>TC10982 weakly similar to UP|Q9AWB6 (Q9AWB6) Phospholipase PLDb2, partial
(10%)
Length = 586
Score = 23.9 bits (50), Expect = 9.3
Identities = 15/63 (23%), Positives = 24/63 (37%)
Frame = -1
Query: 36 KWNLVAISKAIIYGISLGP*MCL*IH*IRKDTFPLEALVLREQHEREGNRWWEDAKAKDC 95
+W ++++ + YG+ L L L+ R H R RW + A C
Sbjct: 154 RWPFISVTSSATYGLQL---------------CSLNDLI-RLTHSRLSGRWKHSSTAPIC 23
Query: 96 GLH 98
LH
Sbjct: 22 SLH 14
>TC14350 similar to UP|PSAL_SPIOL (Q41385) Photosystem I reaction center
subunit XI, chloroplast precursor (PSI-L) (PSI subunit
V), partial (94%)
Length = 1035
Score = 23.9 bits (50), Expect = 9.3
Identities = 16/47 (34%), Positives = 23/47 (48%), Gaps = 1/47 (2%)
Frame = -1
Query: 72 ALVLREQHEREGNRWWEDAKAKDCGL-HKGNVKQDVGAQLNGNGAAM 117
AL ++ EGNRW + + G+ +G VK D+ G AAM
Sbjct: 180 ALTVKVNCLLEGNRWSGEEPERPLGVTREGLVKVDLS*LAIGEAAAM 40
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.344 0.153 0.534
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,352,918
Number of Sequences: 28460
Number of extensions: 26403
Number of successful extensions: 335
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 332
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 334
length of query: 129
length of database: 4,897,600
effective HSP length: 80
effective length of query: 49
effective length of database: 2,620,800
effective search space: 128419200
effective search space used: 128419200
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.6 bits)
S2: 50 (23.9 bits)
Lotus: description of TM0212.5