BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0206b.3
(304 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP085563 35 0.016
BP045387 34 0.027
AV409549 28 1.9
TC16133 similar to UP|Q39449 (Q39449) Specific tissue protein 1,... 28 1.9
TC14886 similar to GB|AAM10290.1|20147147|AY091691 AT3g13930/MDC... 28 2.5
TC10643 similar to UP|BAC84847 (BAC84847) Acidic nuclear phospho... 27 3.3
CB828083 27 4.3
TC8159 27 5.6
TC8160 27 5.6
AV779190 26 7.3
BP048451 26 9.5
BP058840 26 9.5
TC11760 homologue to UP|PRS4_ORYSA (P46466) 26S protease regulat... 26 9.5
TC18516 weakly similar to GB|BAB85647.1|19032343|AB076907 inflor... 26 9.5
>BP085563
Length = 338
Score = 35.0 bits (79), Expect = 0.016
Identities = 20/49 (40%), Positives = 26/49 (52%)
Frame = -2
Query: 83 GLGKDEQGIIEPIKSGMRDPRLGLGKQEEDDFFTAEENIQRKKLDVELE 131
GLGKD G+IEP+++ + R GLG Q+ KKLD LE
Sbjct: 310 GLGKDGSGMIEPVQTQAMENRAGLGSQQ-------------KKLDPSLE 203
>BP045387
Length = 496
Score = 34.3 bits (77), Expect = 0.027
Identities = 17/38 (44%), Positives = 23/38 (59%), Gaps = 1/38 (2%)
Frame = -1
Query: 73 LLQKMGW-KGKGLGKDEQGIIEPIKSGMRDPRLGLGKQ 109
++ KMG+ G GLG+D QGI P+ + R GLG Q
Sbjct: 496 MMAKMGFVDGSGLGRDSQGITTPLSAVRRPKSRGLGSQ 383
>AV409549
Length = 428
Score = 28.1 bits (61), Expect = 1.9
Identities = 14/49 (28%), Positives = 26/49 (52%)
Frame = -2
Query: 172 LAMEFEAHLSSYDHNHRKRFKQMKEMHGGSSRDDRQKREQQRQEREIAK 220
L M+ + L D H+ R + E+H S+ + ++ QRQ++EI +
Sbjct: 319 LRMK*SSRLRRCDSVHKGRTSKCTEIHDRESQPETEENRIQRQKQEIPR 173
>TC16133 similar to UP|Q39449 (Q39449) Specific tissue protein 1, partial
(40%)
Length = 934
Score = 28.1 bits (61), Expect = 1.9
Identities = 27/112 (24%), Positives = 50/112 (44%)
Frame = +3
Query: 76 KMGWKGKGLGKDEQGIIEPIKSGMRDPRLGLGKQEEDDFFTAEENIQRKKLDVELEETEE 135
KM K + + + QG+++ +KS ++ + K +E+ T E I KK+ E E
Sbjct: 129 KMIMKDEEMPEGIQGLLQ-LKSEIKPGKNSEHKCDEEHVVTNNEYIIEKKVFTEELEPRP 305
Query: 136 NVRKREVLAEREQKIQTEVKEIRKVFYCDLCNKQYKLAMEFEAHLSSYDHNH 187
N+ A + + T+ NK+Y +E ++S+YD +H
Sbjct: 306 NIS-----AYDDDHVDTKA------------NKKYMEDLEPRPNISAYDDDH 410
>TC14886 similar to GB|AAM10290.1|20147147|AY091691 AT3g13930/MDC16_5
{Arabidopsis thaliana;}, partial (47%)
Length = 1352
Score = 27.7 bits (60), Expect = 2.5
Identities = 20/88 (22%), Positives = 37/88 (41%)
Frame = +1
Query: 215 EREIAKFAQIADAQKQQRLQLLQESGSEPVSSETRTATPLTDQEQRNALKFGFSSKGSAS 274
E +IAKF + + +E+ + P + P + E + + S G
Sbjct: 670 EGDIAKFKDYQASASEPSAPPAKETSAPPPPKKEVVEEPAREPEPKVSKPSAAPSSGD-- 843
Query: 275 KNVIGPKRQMVAKKQNIPVSSIFNNDSD 302
+ P + +A+++NIP+SSI D
Sbjct: 844 RIFASPLARKLAEEKNIPISSIKGTGPD 927
>TC10643 similar to UP|BAC84847 (BAC84847) Acidic nuclear
phosphoprotein-like protein, partial (5%)
Length = 789
Score = 27.3 bits (59), Expect = 3.3
Identities = 32/140 (22%), Positives = 57/140 (39%), Gaps = 6/140 (4%)
Frame = +2
Query: 98 GMRDPRLGLGKQEEDDFFTAEENIQRKKLDVELEETEENVRKREVLAEREQKIQTEVKEI 157
G+R +LG G+++ED+ EE+ + D E E E R V + ++ K
Sbjct: 8 GLRMMKLGSGEEDEDE--EEEEDSEESSDDDEDESAEGGSRGTLVDEDENDNGRSSRKRT 181
Query: 158 RKVFYC--DLCNKQYKLAMEFEAHLSSYDHNHRKRFKQMKEMHGGSS----RDDRQKREQ 211
RK C + + +L + +S + GG S R + R
Sbjct: 182 RKSAGCSVSVSSTPSQLVQQLNTEVSGVLQD------------GGKSPWEKRQWMRSRIM 325
Query: 212 QRQEREIAKFAQIADAQKQQ 231
Q +E++++ Q D +KQ+
Sbjct: 326 QLEEQQLSYQTQAFDLEKQR 385
>CB828083
Length = 514
Score = 26.9 bits (58), Expect = 4.3
Identities = 13/17 (76%), Positives = 15/17 (87%), Gaps = 1/17 (5%)
Frame = +1
Query: 34 LAEDFR-LPINHRPTEN 49
LAE+FR LPINHR TE+
Sbjct: 430 LAEEFRTLPINHRSTED 480
>TC8159
Length = 671
Score = 26.6 bits (57), Expect = 5.6
Identities = 17/48 (35%), Positives = 27/48 (55%), Gaps = 1/48 (2%)
Frame = +3
Query: 63 QLTSSNIGFKLLQKMGW-KGKGLGKDEQGIIEPIKSGMRDPRLGLGKQ 109
+ ++ G L+ +M + +G+GL K+ QG+ PIK R L LG Q
Sbjct: 153 EFNTTGFG*NLMARMVYVEGEGLWKNCQGMALPIKVIPRPKSLDLGVQ 296
>TC8160
Length = 530
Score = 26.6 bits (57), Expect = 5.6
Identities = 17/48 (35%), Positives = 27/48 (55%), Gaps = 1/48 (2%)
Frame = +2
Query: 63 QLTSSNIGFKLLQKMGW-KGKGLGKDEQGIIEPIKSGMRDPRLGLGKQ 109
+ ++ G L+ +M + +G+GL K+ QG+ PIK R L LG Q
Sbjct: 32 EFNTTGFG*NLMARMVYVEGEGLWKNCQGMALPIKVIPRPKSLDLGVQ 175
>AV779190
Length = 539
Score = 26.2 bits (56), Expect = 7.3
Identities = 15/30 (50%), Positives = 16/30 (53%)
Frame = +2
Query: 6 SSGRQESGGRGRRQFKKEEAHQDSLIGDLA 35
S GR E+ G GRRQ KEE S D A
Sbjct: 80 SDGR*EAEGEGRRQPPKEEIEDHSTEEDRA 169
>BP048451
Length = 400
Score = 25.8 bits (55), Expect = 9.5
Identities = 18/85 (21%), Positives = 36/85 (42%)
Frame = +1
Query: 133 TEENVRKREVLAEREQKIQTEVKEIRKVFYCDLCNKQYKLAMEFEAHLSSYDHNHRKRFK 192
+ +N R R+ L + Q + +VFY D+C + ++ + L + + +
Sbjct: 142 SRDNYRDRKQLTRQIQSSSPWLFNHSEVFYKDICGRMIQIDPKNFHCLFQFRNKIQIDQY 321
Query: 193 QMKEMHGGSSRDDRQKREQQRQERE 217
KE G R + + ++ERE
Sbjct: 322 HKKEEETGKYRSISETKRHHQRERE 396
>BP058840
Length = 464
Score = 25.8 bits (55), Expect = 9.5
Identities = 22/81 (27%), Positives = 33/81 (40%)
Frame = +2
Query: 187 HRKRFKQMKEMHGGSSRDDRQKREQQRQEREIAKFAQIADAQKQQRLQLLQESGSEPVSS 246
HR R + E +GG R+D ++ R R K+ + L S S ++S
Sbjct: 8 HRSRSRSSHEFYGGFFREDERRIFMSRFFR*CISCFHPHTNPKRSTISLSLTSSSLALTS 187
Query: 247 ETRTATPLTDQEQRNALKFGF 267
T L+ Q +R L GF
Sbjct: 188 ST-----LSIQLRRKLLPMGF 235
>TC11760 homologue to UP|PRS4_ORYSA (P46466) 26S protease regulatory subunit
4 homolog (TAT-binding protein homolog 2), partial (27%)
Length = 555
Score = 25.8 bits (55), Expect = 9.5
Identities = 9/35 (25%), Positives = 19/35 (53%)
Frame = -2
Query: 185 HNHRKRFKQMKEMHGGSSRDDRQKREQQRQEREIA 219
+NH ++KQ+K D ++RE + + +I+
Sbjct: 464 NNHNSKYKQVKHRFTEQENDHNRRRETRENQDDIS 360
>TC18516 weakly similar to GB|BAB85647.1|19032343|AB076907 inflorescence and
root apices receptor-like kinase {Arabidopsis
thaliana;}, partial (7%)
Length = 654
Score = 25.8 bits (55), Expect = 9.5
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = -1
Query: 81 GKGLGKDEQGIIEPIKSGMRDPRLGLGKQEEDDF 114
G G D G+I K+G++DP L EDD+
Sbjct: 183 GPGFSDDVLGLIV-FKAGLQDPNHRLSSWNEDDY 85
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.311 0.130 0.351
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,645,414
Number of Sequences: 28460
Number of extensions: 38827
Number of successful extensions: 239
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 238
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 239
length of query: 304
length of database: 4,897,600
effective HSP length: 90
effective length of query: 214
effective length of database: 2,336,200
effective search space: 499946800
effective search space used: 499946800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0206b.3