BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0206a.7
(300 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC11166 weakly similar to UP|Q9FXJ3 (Q9FXJ3) F1K23.17, partial (... 87 3e-18
TC11748 weakly similar to UP|O82076 (O82076) Lipase homolog (Fra... 80 4e-16
BP058564 77 3e-15
AV773399 62 2e-10
BP059660 57 3e-09
AW720418 53 7e-08
TC13503 similar to UP|Q8W0Y7 (Q8W0Y7) Enod8.3 (Fragment), partia... 49 8e-07
TC12732 similar to UP|Q9FJ41 (Q9FJ41) GDSL-motif lipase/hydrolas... 49 1e-06
BP054550 47 4e-06
TC15680 weakly similar to UP|Q7Y1X1 (Q7Y1X1) ENSP-like protein, ... 45 1e-05
TC9321 similar to UP|O04320 (O04320) Proline-rich protein APG is... 44 3e-05
AV425516 44 3e-05
BI419094 43 7e-05
TC17805 weakly similar to UP|O82681 (O82681) Lanatoside 15'-O-ac... 42 1e-04
TC17831 similar to UP|Q94CH8 (Q94CH8) Family II lipase EXL1, par... 42 1e-04
BG662032 39 0.001
TC12378 39 0.001
TC15103 weakly similar to UP|Q9LH73 (Q9LH73) GDSL-motif lipase/h... 33 0.045
TC9315 32 0.10
TC10246 32 0.13
>TC11166 weakly similar to UP|Q9FXJ3 (Q9FXJ3) F1K23.17, partial (15%)
Length = 495
Score = 87.4 bits (215), Expect = 3e-18
Identities = 50/122 (40%), Positives = 69/122 (55%), Gaps = 5/122 (4%)
Frame = +2
Query: 159 DQFECLITHNTLIEYFNEQLKHVIETLRKKFLQANIMYFDYYNDVKRLFQAPEQYGVCCI 218
DQ C+ N E++N++L+ I LR+ ANI+Y DYYN L+Q P+++G +
Sbjct: 2 DQAGCVKWLNEFAEFYNQKLQLEIHRLRELHPHANIIYADYYNAALPLYQNPQKFGFRGL 181
Query: 219 PIIACYGSSGP*NI---GGGGKP--IVCRDLSKHINWDGAHFTEAAHKLIAKGLVEGPFV 273
I C G GP N G G P I C D S++I WDG H TEAA+ I+ GL+ GP+
Sbjct: 182 EI--CCGMGGPYNYNASAGCGSPGVIACDDPSEYIGWDGIHLTEAAYGFISDGLMNGPYS 355
Query: 274 DP 275
P
Sbjct: 356 FP 361
>TC11748 weakly similar to UP|O82076 (O82076) Lipase homolog (Fragment),
partial (48%)
Length = 679
Score = 80.1 bits (196), Expect = 4e-16
Identities = 53/161 (32%), Positives = 76/161 (46%), Gaps = 7/161 (4%)
Frame = +3
Query: 122 ALIEEGAMELGVLGNFPMGCNALILTVVNSSTKKEDYDQFECLITHNTLIEYFNEQLKHV 181
+L EGA + V P+GC IL + +K+D D F CL ++N+ FNE L H
Sbjct: 27 SLYNEGARKFWVHNTGPLGCLPKILALA----QKKDLDLFGCLSSYNSAARLFNEALYHS 194
Query: 182 IETLRKKFLQANIMYFDYYNDVKRLFQAPEQYGVCCIPIIACYGSSGP-------*NIGG 234
+ LR + A ++Y D Y L +YG P+ C G GP G
Sbjct: 195 SQKLRTRLKDATLVYVDIYAIKHDLIANATKYGF-SNPLTVCCGFGGPPYNFDLRVTCGQ 371
Query: 235 GGKPIVCRDLSKHINWDGAHFTEAAHKLIAKGLVEGPFVDP 275
G VC + S+++NWDG H TEAA+ IA ++ + P
Sbjct: 372 PGYQ-VCDEGSRYVNWDGTHHTEAANTFIASKILSTDYSTP 491
>BP058564
Length = 468
Score = 77.4 bits (189), Expect = 3e-15
Identities = 39/118 (33%), Positives = 65/118 (55%), Gaps = 5/118 (4%)
Frame = -2
Query: 163 CLITHNTLIEYFNEQLKHVIETLRKKFLQANIMYFDYYNDVKRLFQAPEQYGVCCIPIIA 222
CL N E+ ++ L+ + LR + NI+Y D++N +++++PE +G +
Sbjct: 467 CLKWLNMFHEHHDDLLQIELNQLRVLYPLTNIIYADFFNAALQIYKSPENFGFGGNALKV 288
Query: 223 CYGSSGP*N-----IGGGGKPIVCRDLSKHINWDGAHFTEAAHKLIAKGLVEGPFVDP 275
C G GP N + G I C D S++++WDG H TEAA++ I KGL++G + P
Sbjct: 287 CCGGGGPYNYNDTAVCGNSGVIACDDPSQYVSWDGYHLTEAAYRWITKGLLDGSYTTP 114
>AV773399
Length = 492
Score = 61.6 bits (148), Expect = 2e-10
Identities = 42/107 (39%), Positives = 53/107 (49%), Gaps = 14/107 (13%)
Frame = -1
Query: 185 LRKKFLQANIMYFDYYNDVKRLFQAPEQYGVCCIPIIACYGSSGP*N----IGGGGKPIV 240
LRKK A+I Y D Y+ VK + + P+ AC G G N IG G K V
Sbjct: 492 LRKKLHSASITYVDVYS-VKYSLISQAKTHAFGEPLRACCGHGGKYNYYIHIGCGAKLKV 316
Query: 241 ----------CRDLSKHINWDGAHFTEAAHKLIAKGLVEGPFVDPPL 277
C D S +NWDG HFTEAA+K + +V+G F DPP+
Sbjct: 315 HGQEILLGKPCEDPSLSVNWDGVHFTEAANKWVFDQIVDGSFSDPPI 175
>BP059660
Length = 501
Score = 57.4 bits (137), Expect = 3e-09
Identities = 34/101 (33%), Positives = 50/101 (48%), Gaps = 8/101 (7%)
Frame = -3
Query: 185 LRKKFLQANIMYFDYYNDVKRLFQAPEQYGVCCIPIIACYGSSGP*NIGGGGKPIV---- 240
LR+ +A Y D Y L + G P+ C GS I G K +V
Sbjct: 493 LRRNLPKAKFTYVDVYTAKYELISNASKQGFVN-PLEVCCGSYYGYRIDCGKKAVVNGTV 317
Query: 241 ----CRDLSKHINWDGAHFTEAAHKLIAKGLVEGPFVDPPL 277
C++ S+HI+WDG H+T+AA+K +AK + +G DPP+
Sbjct: 316 YGNPCKNPSQHISWDGVHYTQAANKWVAKHIRDGSLSDPPV 194
>AW720418
Length = 500
Score = 52.8 bits (125), Expect = 7e-08
Identities = 36/117 (30%), Positives = 56/117 (47%)
Frame = +3
Query: 84 FLEEINKNDIFIFMKNKSFTQLREIVQVVESITNTTMIALIEEGAMELGVLGNFPMGCNA 143
++ +I +ND+ + S QLR + + S + + L EGA + P+GC
Sbjct: 93 YMFDIGQNDLSYGFQYSSEEQLRASLPNILSQFSQAVEQLYXEGARVFWIHNTGPIGCLP 272
Query: 144 LILTVVNSSTKKEDYDQFECLITHNTLIEYFNEQLKHVIETLRKKFLQANIMYFDYY 200
L S KK + D C+I+ N + FN QLK+ + LRKK A ++Y D Y
Sbjct: 273 LNFLAYGKS-KKGNVDANGCVISQNGIAHEFNLQLKNQVLQLRKKLPLAKLIYVDVY 440
>TC13503 similar to UP|Q8W0Y7 (Q8W0Y7) Enod8.3 (Fragment), partial (30%)
Length = 705
Score = 49.3 bits (116), Expect = 8e-07
Identities = 28/87 (32%), Positives = 45/87 (51%)
Frame = -2
Query: 128 AMELGVLGNFPMGCNALILTVVNSSTKKEDYDQFECLITHNTLIEYFNEQLKHVIETLRK 187
A G+ P+GC ILT + K + D + C +N + +YFN++LK + LR+
Sbjct: 368 ARSFGIHNT*PIGCLPAILT----NFPKAERDHYGCAT*YNEVAKYFNQKLKEALAQLRR 201
Query: 188 KFLQANIMYFDYYNDVKRLFQAPEQYG 214
+ A I+Y D Y+ F+ E+YG
Sbjct: 200 ELPLAAIIYVDIYSPKLDHFRNLEKYG 120
>TC12732 similar to UP|Q9FJ41 (Q9FJ41) GDSL-motif lipase/hydrolase-like
protein, partial (5%)
Length = 490
Score = 48.9 bits (115), Expect = 1e-06
Identities = 36/116 (31%), Positives = 52/116 (44%), Gaps = 4/116 (3%)
Frame = +1
Query: 149 VNSSTKKEDYDQFECLITHNTLIEYFNEQLKHVIETLRKKFLQANIMYFDYYNDVKRLFQ 208
V S ED Q C NT + +N +L+ +E+LR A + YFD Y +
Sbjct: 1 VTISNNTEDLFQRVCNDQQNTDSQVYNTKLQSHLESLRATLKGAKVGYFDIYTPFMDMIH 180
Query: 209 APEQYGVCCIPIIACYGSS----GP*NIGGGGKPIVCRDLSKHINWDGAHFTEAAH 260
+P +YG + C GS GP + P C D SK++ WD H T+A +
Sbjct: 181 SPAKYGF-EQTLEGCCGSGLVEMGP--VCNAFAP-TCPDASKYLFWDAVHPTQAGY 336
>BP054550
Length = 417
Score = 47.0 bits (110), Expect = 4e-06
Identities = 28/74 (37%), Positives = 37/74 (49%), Gaps = 7/74 (9%)
Frame = -3
Query: 215 VCCIPIIACYGSSGP*NIGG----GGKPIV---CRDLSKHINWDGAHFTEAAHKLIAKGL 267
+CC Y P N G GK I C D S++I WDG + EAA++ IA +
Sbjct: 412 ICC----GYYKDGHPVNCGKKEIINGKEIYAGPCEDPSQYIRWDGVPYPEAANRWIAHRI 245
Query: 268 VEGPFVDPPLKSSH 281
+ G F DPPL +H
Sbjct: 244 LHGSFSDPPLPLTH 203
>TC15680 weakly similar to UP|Q7Y1X1 (Q7Y1X1) ENSP-like protein, partial
(7%)
Length = 641
Score = 45.4 bits (106), Expect = 1e-05
Identities = 17/37 (45%), Positives = 28/37 (74%)
Frame = +1
Query: 241 CRDLSKHINWDGAHFTEAAHKLIAKGLVEGPFVDPPL 277
C++ S+ ++WDG H++E A+ LIAK ++ G F DPP+
Sbjct: 88 CKNPSEFLSWDGVHYSEEANLLIAKQILSGAFSDPPV 198
>TC9321 similar to UP|O04320 (O04320) Proline-rich protein APG isolog,
partial (37%)
Length = 541
Score = 44.3 bits (103), Expect = 3e-05
Identities = 30/118 (25%), Positives = 53/118 (44%), Gaps = 12/118 (10%)
Frame = +2
Query: 163 CLITHNTLIEYFNEQLKHVIETLRKKFLQANIMYFDYYNDVKRLFQAPEQYGV------C 216
C+ NT + FN+++ L+K+ I+ FD Y + L Q P +G C
Sbjct: 11 CVSRFNTDAQAFNKKINSAAAKLQKQHPGLKIVIFDIYKPLYDLVQTPSNFGFAEARRGC 190
Query: 217 C------IPIIACYGSSGP*NIGGGGKPIVCRDLSKHINWDGAHFTEAAHKLIAKGLV 268
C + C P +IG C + ++++ WD H +EAA++++A L+
Sbjct: 191 CGTGTVETTSLLC----NPKSIG------TCSNATQYVFWDSVHPSEAANQVLADALI 334
>AV425516
Length = 415
Score = 44.3 bits (103), Expect = 3e-05
Identities = 26/73 (35%), Positives = 35/73 (47%), Gaps = 13/73 (17%)
Frame = -3
Query: 218 IPIIACYGSSGP*NIGGG----------GKPIV---CRDLSKHINWDGAHFTEAAHKLIA 264
IP+I C G G N G G+ I C S + WDG H+TEAA+K++
Sbjct: 380 IPLINCCGYGGKYNYTDGVACGQNIKVDGEEIFVGSCESPSTKVIWDGTHYTEAANKIVF 201
Query: 265 KGLVEGPFVDPPL 277
+ G F DPP+
Sbjct: 200 DLISTGAFSDPPI 162
>BI419094
Length = 585
Score = 42.7 bits (99), Expect = 7e-05
Identities = 32/132 (24%), Positives = 57/132 (42%), Gaps = 1/132 (0%)
Frame = +3
Query: 138 PMGCNALILTVVNSSTKKEDYDQFECLITHNTLIEYFNEQLKHVIETLRKKFLQANIMYF 197
P+GC +T+ +D +C+ N FN +L ++L+K ++
Sbjct: 15 PVGCLPAAITLFG-------HDSNQCVARLNNDAVNFNRKLNTTSQSLQKSLPGLKLVLL 173
Query: 198 DYYNDVKRLFQAPEQYGVCCIPIIACYGSSGP*NIGGGGKPI-VCRDLSKHINWDGAHFT 256
D Y + L P + G C +I K I C + S+++ WDG H +
Sbjct: 174 DIYQPLYDLVTKPSENGFAEARRACCGTGLLETSILCNQKSIGTCANASEYVFWDGFHPS 353
Query: 257 EAAHKLIAKGLV 268
EAA++++A L+
Sbjct: 354 EAANQVLAGDLI 389
>TC17805 weakly similar to UP|O82681 (O82681) Lanatoside
15'-O-acetylesterase precursor, partial (12%)
Length = 484
Score = 42.4 bits (98), Expect = 1e-04
Identities = 15/36 (41%), Positives = 23/36 (63%)
Frame = +3
Query: 241 CRDLSKHINWDGAHFTEAAHKLIAKGLVEGPFVDPP 276
C S +++WDG H+ EAA+ +A ++ G F DPP
Sbjct: 150 CEKPSMYVSWDGVHYAEAANHWVANRILNGSFTDPP 257
>TC17831 similar to UP|Q94CH8 (Q94CH8) Family II lipase EXL1, partial (16%)
Length = 536
Score = 42.0 bits (97), Expect = 1e-04
Identities = 27/113 (23%), Positives = 52/113 (45%), Gaps = 10/113 (8%)
Frame = +2
Query: 167 HNTLIEYFNEQLKHVIETLRKKFLQANIMYFDYYNDVKRLFQAPEQYGV------CC--- 217
+N E FN +L +++L +K+ + ++Y D YN + + ++YG CC
Sbjct: 2 YNEAAELFNSKLSKEMKSLNQKYPNSRMVYIDVYNPLLDIIINHKKYGYNVEDKGCCGTG 181
Query: 218 -IPIIACYGSSGP*NIGGGGKPIVCRDLSKHINWDGAHFTEAAHKLIAKGLVE 269
I ++ P C ++ +++ WD H TE+A+K I ++E
Sbjct: 182 LIEVVFLCNRLVP----------TCPNVLEYVFWDSFHPTESAYKKIVAPILE 310
>BG662032
Length = 421
Score = 38.9 bits (89), Expect = 0.001
Identities = 33/125 (26%), Positives = 53/125 (42%), Gaps = 3/125 (2%)
Frame = +1
Query: 133 VLGNF-PMGCNALILTVVNSSTKKEDYDQFECLITHNTLIEYFNEQLKHVIETLRKKFLQ 191
V+GN P+GC T+ ++ EC+ N L +N +LK ++ L
Sbjct: 67 VIGNVGPIGCIPYQKTI-------NQLNENECVDLPNKLALQYNARLKDLMAELNDNLPG 225
Query: 192 ANIMYFDYYNDVKRLFQAPEQYGVCCIPIIACYGSSGP*N--IGGGGKPIVCRDLSKHIN 249
A + + Y+ V L + ++YG AC G+ G + G +C D KH+
Sbjct: 226 ATFVVANVYDLVMELIKNYDKYGFTT-STKACCGNGGQFAGIVPCGPTSSICTDRYKHVF 402
Query: 250 WDGAH 254
WD H
Sbjct: 403 WDPYH 417
>TC12378
Length = 464
Score = 38.5 bits (88), Expect = 0.001
Identities = 29/98 (29%), Positives = 44/98 (44%), Gaps = 2/98 (2%)
Frame = +1
Query: 175 NEQLKHVIETLRKKFLQANIMYFDYYNDVKRLFQAPEQYGVCCIPIIACYGSSGP*NIGG 234
N QL H+I+ L KKF + + + P+ YG I C GP N G
Sbjct: 1 NPQLLHMIQALNKKFGREVFVAANTGQMHNDFITNPKAYGFTTSEIACC--GQGPYNGIG 174
Query: 235 GGKPI--VCRDLSKHINWDGAHFTEAAHKLIAKGLVEG 270
P+ +C + + WD H +E A+KLI + ++ G
Sbjct: 175 LCTPLSNLCPHRNLYAFWDPFHPSEKANKLIVEAIMSG 288
>TC15103 weakly similar to UP|Q9LH73 (Q9LH73) GDSL-motif
lipase/hydrolase-like protein, partial (9%)
Length = 544
Score = 33.5 bits (75), Expect = 0.045
Identities = 21/110 (19%), Positives = 50/110 (45%), Gaps = 3/110 (2%)
Frame = +1
Query: 163 CLITHNTLIEYFNEQLKHVIETLRKKFLQANIMYFDYYNDVKRLFQAPEQYGVCCIPIIA 222
C +N + FN +L +++ + K+ ++ + Y+ + ++ P +G +
Sbjct: 7 CSEEYNHVALEFNGKLGLLVKKMNKELPGLQLVDANAYDMLLQIVTQPSYFGFEVAGVGC 186
Query: 223 CYGSSGP*NIG---GGGKPIVCRDLSKHINWDGAHFTEAAHKLIAKGLVE 269
C +G +G P C D +K++ WD H ++ ++++ L+E
Sbjct: 187 C--GTGRFEMGYMCDPKSPFTCTDANKYVFWDAFHPSQKTSQIVSNYLIE 330
>TC9315
Length = 632
Score = 32.3 bits (72), Expect = 0.10
Identities = 19/73 (26%), Positives = 35/73 (47%), Gaps = 3/73 (4%)
Frame = +3
Query: 200 YNDVKRLFQAPEQYGVCCIPIIACYGSSGP*NIG---GGGKPIVCRDLSKHINWDGAHFT 256
Y + ++ + P+ YG + C ++G +G G C D SK + WD H T
Sbjct: 15 YYIMMQIIRKPDLYGFESTSLACC--ATGMFEMGYACSRGSMFSCTDASKFVFWDSFHPT 188
Query: 257 EAAHKLIAKGLVE 269
E + ++AK +++
Sbjct: 189 EKTNGIVAKYVMQ 227
>TC10246
Length = 525
Score = 32.0 bits (71), Expect = 0.13
Identities = 20/63 (31%), Positives = 29/63 (45%), Gaps = 3/63 (4%)
Frame = +3
Query: 210 PEQYGVCCIPIIACYGSSGP*NIG---GGGKPIVCRDLSKHINWDGAHFTEAAHKLIAKG 266
P QYG + C ++G +G C D SK++ WD H TE + +IA
Sbjct: 27 PAQYGFQVASMACC--ATGMFEMGYACSRASLFSCMDASKYVFWDSFHTTEKTYGIIANY 200
Query: 267 LVE 269
LV+
Sbjct: 201 LVK 209
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.342 0.152 0.495
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,322,385
Number of Sequences: 28460
Number of extensions: 69412
Number of successful extensions: 467
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 458
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 459
length of query: 300
length of database: 4,897,600
effective HSP length: 90
effective length of query: 210
effective length of database: 2,336,200
effective search space: 490602000
effective search space used: 490602000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (22.0 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0206a.7