BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0206a.3
(552 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC12965 weakly similar to GB|AAP49518.1|31376383|BT008756 At3g62... 317 3e-87
TC9647 similar to UP|Q9LGZ2 (Q9LGZ2) ESTs AU091298(C61241), part... 128 3e-30
TC10618 weakly similar to GB|AAP49518.1|31376383|BT008756 At3g62... 60 3e-17
AV413701 47 6e-06
AW719706 35 0.041
BI418438 34 0.054
AV409090 34 0.070
TC16495 similar to UP|Q9LUA5 (Q9LUA5) Genomic DNA, chromosome 3,... 33 0.092
TC11490 similar to UP|O48566 (O48566) CLP protease regulatory su... 33 0.12
BP036655 31 0.60
BP065436 30 0.78
CB827913 30 1.0
TC15861 similar to UP|S61G_SCHPO (Q09827) Probable protein trans... 30 1.0
BP035202 30 1.3
BP029161 29 1.7
CB829527 29 1.7
AV426390 29 1.7
TC8341 similar to UP|Q94FX1 (Q94FX1) Heme oxygenase 1 (Fragment)... 29 2.3
BP067355 29 2.3
TC20221 28 3.0
>TC12965 weakly similar to GB|AAP49518.1|31376383|BT008756 At3g62130
{Arabidopsis thaliana;}, partial (18%)
Length = 471
Score = 317 bits (812), Expect = 3e-87
Identities = 155/157 (98%), Positives = 155/157 (98%)
Frame = +1
Query: 57 SISFSPLTHSILSLCLSSSSSSSYVVVLSLVIFTHHFLISSSMASTHNHNNNNNGCTTHI 116
SISFSPLTHSILSLCLSSSSSSSYVV LSLVIFTHHFLISSSMASTHNHNNNNNGCTTHI
Sbjct: 1 SISFSPLTHSILSLCLSSSSSSSYVVALSLVIFTHHFLISSSMASTHNHNNNNNGCTTHI 180
Query: 117 PKKPKLSPLSSSPFITPSEIQSEFSHHDPSVARINNGSFGCCSASVTAAQHDWQLKYLRQ 176
PKKPKLSPLSSSPFITPSEIQSEFSHHDPSVARINNGSFGCC ASVTAAQHDWQLKYLRQ
Sbjct: 181 PKKPKLSPLSSSPFITPSEIQSEFSHHDPSVARINNGSFGCCPASVTAAQHDWQLKYLRQ 360
Query: 177 PDNFYFNHLKQGILRSRTIIKDLVNADHLDEISIVDN 213
PDNFYFNHLKQGILRSRTIIKDLVNADHLDEISIVDN
Sbjct: 361 PDNFYFNHLKQGILRSRTIIKDLVNADHLDEISIVDN 471
>TC9647 similar to UP|Q9LGZ2 (Q9LGZ2) ESTs AU091298(C61241), partial (9%)
Length = 592
Score = 128 bits (321), Expect = 3e-30
Identities = 60/60 (100%), Positives = 60/60 (100%)
Frame = +3
Query: 493 AFGVEVPIYYRPPRDGEVEPVTGYARISHQVYNKVDDYYKFRDAVIQLVDKGFTCALLSD 552
AFGVEVPIYYRPPRDGEVEPVTGYARISHQVYNKVDDYYKFRDAVIQLVDKGFTCALLSD
Sbjct: 3 AFGVEVPIYYRPPRDGEVEPVTGYARISHQVYNKVDDYYKFRDAVIQLVDKGFTCALLSD 182
>TC10618 weakly similar to GB|AAP49518.1|31376383|BT008756 At3g62130
{Arabidopsis thaliana;}, partial (16%)
Length = 497
Score = 60.1 bits (144), Expect(2) = 3e-17
Identities = 29/50 (58%), Positives = 37/50 (74%)
Frame = +1
Query: 463 CASMVMVGLPTCLGVQSDSDALKLRTHLREAFGVEVPIYYRPPRDGEVEP 512
CASM+MVGLP+ L V SD DA +LR++LR +EVPIYY+ RD E +P
Sbjct: 1 CASMIMVGLPSRLCVLSDDDASRLRSYLRVYHAIEVPIYYQALRDAERDP 150
Score = 45.1 bits (105), Expect(2) = 3e-17
Identities = 18/36 (50%), Positives = 28/36 (77%)
Frame = +2
Query: 513 VTGYARISHQVYNKVDDYYKFRDAVIQLVDKGFTCA 548
+TGY RIS+QVYNK DDY++ ++A+ QL++ C+
Sbjct: 170 ITGYVRISYQVYNKDDDYHRLKNAINQLLEDRKICS 277
>AV413701
Length = 416
Score = 47.4 bits (111), Expect = 6e-06
Identities = 25/80 (31%), Positives = 45/80 (56%), Gaps = 1/80 (1%)
Frame = +3
Query: 296 KKVRLAVIDHVTSMPCVVIPVKELVKICREEGVDRVFVDAAHSIGCTDVDMKEIGADFYT 355
KK ++ V+ HV+++ V+P++++ + G +V VDA S+ VD++ + DF
Sbjct: 105 KKTKIVVVHHVSNVLASVLPIRDIAHWAHDVGA-KVLVDACQSVPHMVVDVQSLNVDFLV 281
Query: 356 SNLHKWFFC-PPSIAFLYSR 374
++ HK C P I FLY +
Sbjct: 282 ASSHK--MCGPTGIGFLYGK 335
>AW719706
Length = 432
Score = 34.7 bits (78), Expect = 0.041
Identities = 30/111 (27%), Positives = 49/111 (44%), Gaps = 5/111 (4%)
Frame = +2
Query: 45 THPLVSVPLIIYSISFS--PLTHSILSLCLSSSSSSSYVVVL---SLVIFTHHFLISSSM 99
TH S+PL +YS+ T S + L S++S++ S +H +
Sbjct: 26 TH-FTSLPLPLYSLRRR**TKTMSYVPPHLRSATSTTVATTTRPSSSATLDNHHHTKLAF 202
Query: 100 ASTHNHNNNNNGCTTHIPKKPKLSPLSSSPFITPSEIQSEFSHHDPSVARI 150
+S+H++NNNNN ++ P++ P P PSE S + RI
Sbjct: 203 SSSHSNNNNNNSSLSNGPRRTSALPDPLFPLWQPSERVSRMNPQQIEEVRI 355
>BI418438
Length = 431
Score = 34.3 bits (77), Expect = 0.054
Identities = 32/142 (22%), Positives = 68/142 (47%), Gaps = 16/142 (11%)
Frame = +3
Query: 41 SKVKTHPLVSVPLIIYSISFSPLTHSILSLCLSSSSS------SSYVVVLSLVIFTHHFL 94
SK +++P V + I SIS P T ++L + + +++ S+++ + L+ + L
Sbjct: 12 SKFQSNPTHIV*ITI-SISIKP*TFNLLKVPIKPTTTTFSLTISTFLTMSPLLPLSFLLL 188
Query: 95 ISSSMASTHNHNNNNNGCTTHIPKKPKLSPLSSSPFIT--------PSEIQSEFSH--HD 144
I + +++H+HNN++ H P L +++P I P + +S SH +
Sbjct: 189 ILTLSSASHSHNNHH-----HHSPTPSLPDATAAPEIQQACKATRFPQQCESSLSHLPPN 353
Query: 145 PSVARINNGSFGCCSASVTAAQ 166
P+ ++ + S ++ AQ
Sbjct: 354 PTSLQLLQSAISATSTNLATAQ 419
>AV409090
Length = 436
Score = 33.9 bits (76), Expect = 0.070
Identities = 32/101 (31%), Positives = 46/101 (44%), Gaps = 13/101 (12%)
Frame = +3
Query: 65 HSILSLCLSSSSSSSYVVVLSLVIFTHHFLISSSMASTHNHNNN------------NNGC 112
H + LC SS+S + V++L LV + H +IS + A NNN N C
Sbjct: 9 HLKMLLCSSSTSITMTVLLLLLVAMSFH-MISETQAQQEYLNNNQLDCDNTHNSTYGNVC 185
Query: 113 TTHIPKKPKLSPLSSSP-FITPSEIQSEFSHHDPSVARINN 152
+ + L+ SSSP + TPS I + VA+ NN
Sbjct: 186 NSVTSCQSYLTFKSSSPEYNTPSSISYLLNSTPSLVAKSNN 308
>TC16495 similar to UP|Q9LUA5 (Q9LUA5) Genomic DNA, chromosome 3, P1 clone:
MPE11, partial (14%)
Length = 919
Score = 33.5 bits (75), Expect = 0.092
Identities = 40/137 (29%), Positives = 57/137 (41%), Gaps = 19/137 (13%)
Frame = +1
Query: 62 PLTHSILSL----CLSSSSSSSYVVVLSLVIFTHH-FLISSSMASTHNHNNNNNGCTTHI 116
PL LSL +++S+ S + +L F+ F IS M + N+NNNNN +
Sbjct: 232 PLPSHFLSLHSNNIINNSTHHSVCFINTLFAFSFFCFSISFQMGESCNNNNNNNDTRKRV 411
Query: 117 PK--------KPKLSPLSSSPFITPSE------IQSEFSHHDPSVARINNGSFGCCSASV 162
+ + KL LSS P + E EFS + R+++G SA V
Sbjct: 412 REDNSEADSPESKLPRLSSGPDVNSPESGLARVSSGEFSETE----RVDSGDAVLDSAGV 579
Query: 163 TAAQHDWQLKYLRQPDN 179
Q D L L DN
Sbjct: 580 NEIQDD-LLNMLDDADN 627
>TC11490 similar to UP|O48566 (O48566) CLP protease regulatory subunit CLPX
precursor, partial (4%)
Length = 528
Score = 33.1 bits (74), Expect = 0.12
Identities = 33/104 (31%), Positives = 43/104 (40%), Gaps = 17/104 (16%)
Frame = +1
Query: 39 SLSKVKTHPLVSVPLIIY-----SISFS----------PLTHSILSLCLSSSSSSSYVVV 83
S+S + +PL+ + SIS S PL H + +L S SSS +
Sbjct: 31 SISSSSQKQKIQIPLLSHFFHQISISMSRIIAAWKTQKPLLHHLPTLSPSPSSSRA---- 198
Query: 84 LSLVIFTHHFLISSSMASTHNHNNNN--NGCTTHIPKKPKLSPL 125
THHF +SS H HNNNN T H K +PL
Sbjct: 199 ------THHFTLSSR----HRHNNNNFHFATTQHRCKSYSTAPL 300
>BP036655
Length = 556
Score = 30.8 bits (68), Expect = 0.60
Identities = 25/73 (34%), Positives = 38/73 (51%), Gaps = 4/73 (5%)
Frame = +1
Query: 60 FSPLTHSILSLCLSSSSSSSYVVVLSLVI--FTHHFLISS--SMASTHNHNNNNNGCTTH 115
F+P++ S S SSSSSSS+ V +S+ + T L SS S +S H+ +++ TT
Sbjct: 196 FTPISSSTFSTPHSSSSSSSFSVSISVSLSSSTSSSLSSSLISSSSLHSSTTSSSSSTTS 375
Query: 116 IPKKPKLSPLSSS 128
P SS+
Sbjct: 376 SSSPSSERPSSSN 414
>BP065436
Length = 524
Score = 30.4 bits (67), Expect = 0.78
Identities = 15/51 (29%), Positives = 26/51 (50%)
Frame = -3
Query: 102 THNHNNNNNGCTTHIPKKPKLSPLSSSPFITPSEIQSEFSHHDPSVARINN 152
T ++ +NN+ +P+L P+S + F +P + FSHH V + N
Sbjct: 441 TCSNTDNNSLLRKFASPQPRLGPISETRFSSPRKPFLPFSHHHVEVCLVVN 289
>CB827913
Length = 567
Score = 30.0 bits (66), Expect = 1.0
Identities = 24/88 (27%), Positives = 36/88 (40%), Gaps = 9/88 (10%)
Frame = -1
Query: 6 MLVECNKCKVFNNKHLFGFFNFGFPCGWIHVLCSLSKVKTHPLVSVPLIIYSISFSPLT- 64
+ + C C FN HLF FN P +IH+ C + S L I+ S+ P+
Sbjct: 255 LCIFCFNCLNFNTSHLFRSFNCLSPFLFIHIRC-------FSINSYCLGIFHTSYIPVNP 97
Query: 65 --------HSILSLCLSSSSSSSYVVVL 84
HS L + S+ + +VL
Sbjct: 96 IGLVLLRCHSSLPFSIRRISTLPFSIVL 13
>TC15861 similar to UP|S61G_SCHPO (Q09827) Probable protein transport
protein SEC61 gamma subunit, partial (21%)
Length = 801
Score = 30.0 bits (66), Expect = 1.0
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = +2
Query: 2 NILFMLVECNKCKVFNNKHLFGFFNFGFPCGWI 34
+I +L+ FN + LF F + PCGWI
Sbjct: 308 DICLILLPLGSWVSFNLRLLFSFIRYNIPCGWI 406
>BP035202
Length = 469
Score = 29.6 bits (65), Expect = 1.3
Identities = 24/67 (35%), Positives = 32/67 (46%)
Frame = +1
Query: 21 LFGFFNFGFPCGWIHVLCSLSKVKTHPLVSVPLIIYSISFSPLTHSILSLCLSSSSSSSY 80
L FFNF W +LSKVK P + I S PL ++LS+C SSS S
Sbjct: 259 LLDFFNF-----W-----TLSKVKDSPSTASCSSIIGCSSLPLFFALLSVCESSSKFLSN 408
Query: 81 VVVLSLV 87
+L ++
Sbjct: 409 QCMLPVL 429
>BP029161
Length = 528
Score = 29.3 bits (64), Expect = 1.7
Identities = 16/45 (35%), Positives = 23/45 (50%)
Frame = +3
Query: 12 KCKVFNNKHLFGFFNFGFPCGWIHVLCSLSKVKTHPLVSVPLIIY 56
+C+V NNK L F +F C W LC + K+K ++ IY
Sbjct: 78 QCQVVNNKKLLDFTHFFSKCRW--HLCFIVKLKVCLILDDNFCIY 206
>CB829527
Length = 550
Score = 29.3 bits (64), Expect = 1.7
Identities = 18/45 (40%), Positives = 23/45 (51%), Gaps = 7/45 (15%)
Frame = +3
Query: 92 HFLISSSMASTHNHNNNNNGCTTHIPKK-------PKLSPLSSSP 129
H +SSS + N+NNNNN +P + P SPL SSP
Sbjct: 354 HQDVSSSEDTRDNNNNNNNQNLVKVPGRSVSTLTSPFASPLISSP 488
>AV426390
Length = 399
Score = 29.3 bits (64), Expect = 1.7
Identities = 16/37 (43%), Positives = 21/37 (56%)
Frame = +1
Query: 98 SMASTHNHNNNNNGCTTHIPKKPKLSPLSSSPFITPS 134
S +++NHNNNNN T I P P S +P + PS
Sbjct: 61 SPTNSNNHNNNNNNNTIPI---PIALPSSLTPTLLPS 162
>TC8341 similar to UP|Q94FX1 (Q94FX1) Heme oxygenase 1 (Fragment), partial
(86%)
Length = 1286
Score = 28.9 bits (63), Expect = 2.3
Identities = 16/54 (29%), Positives = 27/54 (49%)
Frame = +3
Query: 118 KKPKLSPLSSSPFITPSEIQSEFSHHDPSVARINNGSFGCCSASVTAAQHDWQL 171
++P+L P S+P+I+ S HH S A+ + GC ++ +A W L
Sbjct: 246 RQPQLFP-KSNPYISSSRASHHHHHHIVSSAQFTSRRRGCYNSIASAK*SRWLL 404
>BP067355
Length = 434
Score = 28.9 bits (63), Expect = 2.3
Identities = 15/45 (33%), Positives = 27/45 (59%)
Frame = -2
Query: 50 SVPLIIYSISFSPLTHSILSLCLSSSSSSSYVVVLSLVIFTHHFL 94
S P+ +++ + + T + + SSSSS S+ + S+VIF H +L
Sbjct: 229 SPPMSLFNAA*NASTSAPECMVSSSSSSRSFTIDGSIVIFVHRYL 95
>TC20221
Length = 509
Score = 28.5 bits (62), Expect = 3.0
Identities = 25/68 (36%), Positives = 37/68 (53%), Gaps = 5/68 (7%)
Frame = -1
Query: 39 SLSKVKTHPLVSVPLIIYSISFSP-LTHSILSLCLS---SSSSSSYVVVLSL-VIFTHHF 93
+L+K K PLV+ P ++ SFS L S L CL+ ++ SS +V SL + +H F
Sbjct: 257 ALAKSKYSPLVTRPRATFAFSFSTHLAPSFLHRCLTNQMNTCSSRI*LVSSLWAV*SHLF 78
Query: 94 LISSSMAS 101
L + AS
Sbjct: 77 LSQAGWAS 54
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.137 0.423
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,778,682
Number of Sequences: 28460
Number of extensions: 208984
Number of successful extensions: 1512
Number of sequences better than 10.0: 61
Number of HSP's better than 10.0 without gapping: 1433
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1486
length of query: 552
length of database: 4,897,600
effective HSP length: 95
effective length of query: 457
effective length of database: 2,193,900
effective search space: 1002612300
effective search space used: 1002612300
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0206a.3