BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0203.8
(124 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18979 similar to UP|Q8LG35 (Q8LG35) EF-hand Ca2+-binding prote... 154 3e-39
AW720025 121 3e-29
TC8059 GB|AAB68399.1|1773321|HAU79736 calmodulin {Helianthus ann... 44 6e-06
TC8422 UP|AAQ63461 (AAQ63461) Calmodulin 4 (Fragment), complete 44 6e-06
TC8060 UP|O49184 (O49184) Calmodulin (ESTS AU081349) (Auxin-regu... 44 6e-06
TC9581 UP|Q9SCA1 (Q9SCA1) Calcium-binding protein, complete 41 5e-05
TC18713 similar to GB|AAL90989.1|19699246|AY090328 AT3g03000/F13... 40 1e-04
TC8061 UP|AAA34013 (AAA34013) Calmodulin, complete 38 6e-04
BP067048 37 0.001
TC10825 similar to GB|AAP68339.1|31711966|BT008900 At1g74740 {Ar... 32 0.024
TC11056 similar to UP|O81390 (O81390) Calcium-dependent protein ... 32 0.041
TC19387 weakly similar to UP|Q9FYK2 (Q9FYK2) F21J9.28, partial (... 31 0.054
TC12734 similar to UP|Q9LM78 (Q9LM78) F2D10.25, partial (11%) 31 0.071
TC13659 homologue to UP|Q9M060 (Q9M060) Eukaryotic translation i... 26 1.7
AU089222 26 1.7
TC8794 similar to UP|O81445 (O81445) Calcineurin B-like protein ... 26 2.3
CB828304 25 3.0
AV765447 25 3.9
TC17991 similar to UP|RIN4_ARATH (Q8GYN5) RPM1-interacting prote... 25 5.1
TC8451 GB|CAA61589.1|897771|LJAS1GENE asparagine synthase (gluta... 25 5.1
>TC18979 similar to UP|Q8LG35 (Q8LG35) EF-hand Ca2+-binding protein CCD1,
partial (84%)
Length = 625
Score = 154 bits (390), Expect = 3e-39
Identities = 75/118 (63%), Positives = 95/118 (79%)
Frame = +3
Query: 1 MAGTGTVNGKGLEFEDLLPVMANKLGGEGLIKELCNGFDMLMDKDRGVITLESLKTNAAV 60
MA T + + +F+D LP+MANKLGG+GLI ELCNGF++LMD ++GVIT +SLK NA
Sbjct: 150 MATTPNSHQQQAQFQDHLPLMANKLGGDGLIDELCNGFNLLMDAEKGVITFDSLKRNAHF 329
Query: 61 LGLQDMKEDELVSMIREGDLDGDGALTQMEFCVLMFRLSPELMEESWIWLEEALQHEL 118
LGLQD+ +++L SMI EGD D DGAL+QMEFCVLMFRLSPELME S +WLE+ L+ E+
Sbjct: 330 LGLQDLSDEDLRSMIVEGDFDRDGALSQMEFCVLMFRLSPELMERSQMWLEQVLEQEM 503
>AW720025
Length = 563
Score = 121 bits (304), Expect = 3e-29
Identities = 56/105 (53%), Positives = 78/105 (73%)
Frame = +3
Query: 13 EFEDLLPVMANKLGGEGLIKELCNGFDMLMDKDRGVITLESLKTNAAVLGLQDMKEDELV 72
EFEDLLPVMA KL E + ELC GF +L D + G+I+ ESL N+A+LG+ M +++
Sbjct: 135 EFEDLLPVMAEKLDVETFVSELCGGFKLLSDPETGLISSESLMRNSALLGMDGMTKEDAE 314
Query: 73 SMIREGDLDGDGALTQMEFCVLMFRLSPELMEESWIWLEEALQHE 117
M+++GDLDGDG+L + EFC+LM RLSP +ME++ WLE+AL+ E
Sbjct: 315 EMVKQGDLDGDGSLNETEFCILMVRLSPGMMEDAESWLEKALEEE 449
>TC8059 GB|AAB68399.1|1773321|HAU79736 calmodulin {Helianthus annuus;} ,
partial (83%)
Length = 836
Score = 44.3 bits (103), Expect = 6e-06
Identities = 29/93 (31%), Positives = 46/93 (49%)
Frame = +2
Query: 3 GTGTVNGKGLEFEDLLPVMANKLGGEGLIKELCNGFDMLMDKDRGVITLESLKTNAAVLG 62
G GT+ +F + L +MA K+ +EL F + G I+ L+ LG
Sbjct: 266 GNGTI-----DFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLG 430
Query: 63 LQDMKEDELVSMIREGDLDGDGALTQMEFCVLM 95
+ + ++E+ MIRE D+DGDG + EF +M
Sbjct: 431 -EKLTDEEVDEMIREADVDGDGQINYEEFVKVM 526
>TC8422 UP|AAQ63461 (AAQ63461) Calmodulin 4 (Fragment), complete
Length = 832
Score = 44.3 bits (103), Expect = 6e-06
Identities = 29/93 (31%), Positives = 46/93 (49%)
Frame = +1
Query: 3 GTGTVNGKGLEFEDLLPVMANKLGGEGLIKELCNGFDMLMDKDRGVITLESLKTNAAVLG 62
G GT+ +F + L +MA K+ +EL F + G I+ L+ LG
Sbjct: 313 GNGTI-----DFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLG 477
Query: 63 LQDMKEDELVSMIREGDLDGDGALTQMEFCVLM 95
+ + ++E+ MIRE D+DGDG + EF +M
Sbjct: 478 -EKLTDEEVDEMIREADVDGDGQINYEEFVKVM 573
Score = 35.8 bits (81), Expect = 0.002
Identities = 27/58 (46%), Positives = 31/58 (52%), Gaps = 1/58 (1%)
Frame = +1
Query: 41 LMDKDR-GVITLESLKTNAAVLGLQDMKEDELVSMIREGDLDGDGALTQMEFCVLMFR 97
L DKD G IT + L T LG Q+ E EL MI E D DG+G + EF LM R
Sbjct: 190 LFDKDGDGSITTKELGTVMRSLG-QNPTEAELQDMINEVDADGNGTIDFPEFLNLMAR 360
>TC8060 UP|O49184 (O49184) Calmodulin (ESTS AU081349) (Auxin-regulated
calmodulin) (Calmodulin TACAM1-1) (Calmodulin TACAM1-2)
(Calmodulin TACAM1-3) (Calmodulin TACAM3-1) (Calmodulin
TACAM3-2) (Calmodulin TACAM3-3) (Calmodulin TACAM4-1)
(CaM protein), complete
Length = 615
Score = 44.3 bits (103), Expect = 6e-06
Identities = 29/93 (31%), Positives = 46/93 (49%)
Frame = +3
Query: 3 GTGTVNGKGLEFEDLLPVMANKLGGEGLIKELCNGFDMLMDKDRGVITLESLKTNAAVLG 62
G GT+ +F + L +MA K+ +EL F + G I+ L+ LG
Sbjct: 246 GNGTI-----DFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLG 410
Query: 63 LQDMKEDELVSMIREGDLDGDGALTQMEFCVLM 95
+ + ++E+ MIRE D+DGDG + EF +M
Sbjct: 411 -EKLTDEEVDEMIREADVDGDGQINYEEFVKVM 506
Score = 37.7 bits (86), Expect = 6e-04
Identities = 34/80 (42%), Positives = 41/80 (50%), Gaps = 1/80 (1%)
Frame = +3
Query: 19 PVMANKLGGEGLIKELCNGFDMLMDKDR-GVITLESLKTNAAVLGLQDMKEDELVSMIRE 77
P MA++L + I E F L DKD G IT + L T LG Q+ E EL MI E
Sbjct: 63 PPMADQLTDDQ-IAEFKEAFS-LFDKDGDGCITTKELGTVMRSLG-QNPTEAELQDMINE 233
Query: 78 GDLDGDGALTQMEFCVLMFR 97
D DG+G + EF LM R
Sbjct: 234 VDADGNGTIDFPEFLNLMAR 293
>TC9581 UP|Q9SCA1 (Q9SCA1) Calcium-binding protein, complete
Length = 1049
Score = 41.2 bits (95), Expect = 5e-05
Identities = 33/94 (35%), Positives = 48/94 (50%), Gaps = 5/94 (5%)
Frame = +3
Query: 7 VNGKGL----EFEDLLPVMANKLGGEGLIKELCNGFDMLMDKDRGVITLESLKTNAAVLG 62
VNG G EF +L + ++ E ++E N FD D G IT+E L+T A LG
Sbjct: 486 VNGDGCVDIDEFGELYQSIMDERDEEEDMREAFNVFDQNGD---GFITVEELRTVLASLG 656
Query: 63 L-QDMKEDELVSMIREGDLDGDGALTQMEFCVLM 95
+ Q ++ MI + D+DGDG + EF +M
Sbjct: 657 IKQGRTVEDCKKMIMKVDVDGDGMVDYKEFKQMM 758
>TC18713 similar to GB|AAL90989.1|19699246|AY090328 AT3g03000/F13E7_5
{Arabidopsis thaliana;}, partial (88%)
Length = 868
Score = 40.0 bits (92), Expect = 1e-04
Identities = 33/104 (31%), Positives = 48/104 (45%), Gaps = 4/104 (3%)
Frame = +1
Query: 8 NGKGL-EFEDLLPVMANKL--GGEGLIKELCNGFDMLMDKD-RGVITLESLKTNAAVLGL 63
N GL EF + + V+A +L +E + D+D G IT L + A LG
Sbjct: 355 NSNGLIEFSEFVAVVAPELLPARSPYTEEQLRQLFRVFDRDGNGFITAAELAHSMAKLG- 531
Query: 64 QDMKEDELVSMIREGDLDGDGALTQMEFCVLMFRLSPELMEESW 107
+ +EL MI+E D DGDG ++ EF +S + SW
Sbjct: 532 HALSAEELTGMIKEADADGDGMISFQEFA---HAISSAAFDNSW 654
Score = 25.0 bits (53), Expect = 3.9
Identities = 12/44 (27%), Positives = 23/44 (52%)
Frame = +1
Query: 52 ESLKTNAAVLGLQDMKEDELVSMIREGDLDGDGALTQMEFCVLM 95
+ + + + L + + EL + R D + DG+LTQ+E L+
Sbjct: 160 KEIMSKKQAVNLDEEQIAELREIFRSFDRNNDGSLTQLELSSLL 291
>TC8061 UP|AAA34013 (AAA34013) Calmodulin, complete
Length = 568
Score = 37.7 bits (86), Expect = 6e-04
Identities = 22/56 (39%), Positives = 34/56 (60%), Gaps = 1/56 (1%)
Frame = +2
Query: 41 LMDKDR-GVITLESLKTNAAVLGLQDMKEDELVSMIREGDLDGDGALTQMEFCVLM 95
L+DKD+ G I+ L+ LG + + ++E+ MIRE D+DGDG + EF +M
Sbjct: 320 LLDKDQNGFISAAELRHVMTNLG-EKLTDEEVDEMIREADVDGDGQINYEEFVKVM 484
>BP067048
Length = 372
Score = 37.0 bits (84), Expect = 0.001
Identities = 19/25 (76%), Positives = 20/25 (80%)
Frame = -2
Query: 94 LMFRLSPELMEESWIWLEEALQHEL 118
L FRLSPELME S + LEEALQ EL
Sbjct: 371 LXFRLSPELMEGSQLXLEEALQQEL 297
>TC10825 similar to GB|AAP68339.1|31711966|BT008900 At1g74740 {Arabidopsis
thaliana;}, partial (32%)
Length = 1091
Score = 32.3 bits (72), Expect = 0.024
Identities = 20/67 (29%), Positives = 33/67 (48%), Gaps = 1/67 (1%)
Frame = +1
Query: 33 ELCNGFDMLMDKDR-GVITLESLKTNAAVLGLQDMKEDELVSMIREGDLDGDGALTQMEF 91
E+ LMD D+ G ++ E LK +G Q + + E+ ++ D+DG+G L EF
Sbjct: 13 EIIKDMFTLMDTDKDGRVSYEELKAGLRKVGSQ-LADQEIKMLMEVADVDGNGVLDYGEF 189
Query: 92 CVLMFRL 98
+ L
Sbjct: 190VAVTIHL 210
>TC11056 similar to UP|O81390 (O81390) Calcium-dependent protein kinase ,
partial (22%)
Length = 675
Score = 31.6 bits (70), Expect = 0.041
Identities = 15/43 (34%), Positives = 27/43 (61%)
Frame = +3
Query: 49 ITLESLKTNAAVLGLQDMKEDELVSMIREGDLDGDGALTQMEF 91
IT E LK+ A +G + + E E+ ++ D+DG+G++ +EF
Sbjct: 6 ITYEELKSGLARIGSR-LSETEVKQLMEAADVDGNGSIDYLEF 131
>TC19387 weakly similar to UP|Q9FYK2 (Q9FYK2) F21J9.28, partial (45%)
Length = 631
Score = 31.2 bits (69), Expect = 0.054
Identities = 23/72 (31%), Positives = 34/72 (46%)
Frame = +1
Query: 26 GGEGLIKELCNGFDMLMDKDRGVITLESLKTNAAVLGLQDMKEDELVSMIREGDLDGDGA 85
GG G KE+ F+M G+I+ + L + LG + + MI D+DGDG
Sbjct: 238 GGGGNSKEIREAFEMYDLDKNGLISAKELHSVMKKLG-EKCSLGDCRKMIGNVDVDGDGN 414
Query: 86 LTQMEFCVLMFR 97
+ EF +M R
Sbjct: 415 VNFEEFKKMMSR 450
>TC12734 similar to UP|Q9LM78 (Q9LM78) F2D10.25, partial (11%)
Length = 936
Score = 30.8 bits (68), Expect = 0.071
Identities = 16/48 (33%), Positives = 28/48 (58%), Gaps = 3/48 (6%)
Frame = +2
Query: 69 DELVSMIREGDLDGDGALTQMEFCVLMFR---LSPELMEESWIWLEEA 113
D+ S R DLDGDG ++ E V F+ LS +++ + W++ ++A
Sbjct: 173 DQFESFFRRADLDGDGRISGAE-AVSFFQGSNLSKQVLAQVWMYADQA 313
>TC13659 homologue to UP|Q9M060 (Q9M060) Eukaryotic translation initiation
factor 6 (EIF-6)-like protein (At3g55620), partial (43%)
Length = 411
Score = 26.2 bits (56), Expect = 1.7
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = +3
Query: 13 EFEDLLPVMANKLGGEGLIKELCNG 37
E D++PV+ +GG +I LC G
Sbjct: 216 ELADVIPVVKTSIGGTRIIGRLCAG 290
>AU089222
Length = 345
Score = 26.2 bits (56), Expect = 1.7
Identities = 13/41 (31%), Positives = 23/41 (55%)
Frame = +3
Query: 51 LESLKTNAAVLGLQDMKEDELVSMIREGDLDGDGALTQMEF 91
+ S + A+ L + +E+ M+ E D+DGDG ++ EF
Sbjct: 123 ISSSELGEALKTLGSVTAEEVQRMMDEIDIDGDGFISYEEF 245
>TC8794 similar to UP|O81445 (O81445) Calcineurin B-like protein 1,
complete
Length = 1077
Score = 25.8 bits (55), Expect = 2.3
Identities = 20/85 (23%), Positives = 35/85 (40%)
Frame = +3
Query: 38 FDMLMDKDRGVITLESLKTNAAVLGLQDMKEDELVSMIREGDLDGDGALTQMEFCVLMFR 97
FD+ K +GVI + + V ED++ R DLD G + + E ++
Sbjct: 405 FDLFDVKKKGVIDFDDFVRSLNVFHPNAALEDKIDFSFRLYDLDSTGFIERQEVKQMLIA 584
Query: 98 LSPELMEESWIWLEEALQHELDNNY 122
L L E +E ++ +D +
Sbjct: 585 L---LCESEMKLADEVVETIIDKTF 650
>CB828304
Length = 489
Score = 25.4 bits (54), Expect = 3.0
Identities = 11/33 (33%), Positives = 19/33 (57%)
Frame = +1
Query: 63 LQDMKEDELVSMIREGDLDGDGALTQMEFCVLM 95
LQ + ++L + D+D DG+LT +E L+
Sbjct: 97 LQVQQLNQLREIFARFDMDSDGSLTMLELAALL 195
>AV765447
Length = 545
Score = 25.0 bits (53), Expect = 3.9
Identities = 11/31 (35%), Positives = 19/31 (60%)
Frame = -1
Query: 44 KDRGVITLESLKTNAAVLGLQDMKEDELVSM 74
KD+G++TLE+L+ +L L+ LV +
Sbjct: 539 KDQGIVTLETLRLLKMILMLKAFSAQPLVGI 447
>TC17991 similar to UP|RIN4_ARATH (Q8GYN5) RPM1-interacting protein 4,
partial (14%)
Length = 598
Score = 24.6 bits (52), Expect = 5.1
Identities = 11/36 (30%), Positives = 17/36 (46%)
Frame = -1
Query: 87 TQMEFCVLMFRLSPELMEESWIWLEEALQHELDNNY 122
T M ++ F++ P WIW E EL+ N+
Sbjct: 370 THMLIALIPFQMDPLNPLRVWIWKEGGYYQELNLNF 263
>TC8451 GB|CAA61589.1|897771|LJAS1GENE asparagine synthase
(glutamine-hydrolysing) {Lotus corniculatus var.
japonicus;} , complete
Length = 2148
Score = 24.6 bits (52), Expect = 5.1
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = +2
Query: 7 VNGKGLEFEDLLPVMANKLG 26
+N K +EFE L+P+ A LG
Sbjct: 1784 INNKPVEFEKLIPMEAPSLG 1843
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.138 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,787,567
Number of Sequences: 28460
Number of extensions: 17381
Number of successful extensions: 117
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 114
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 117
length of query: 124
length of database: 4,897,600
effective HSP length: 79
effective length of query: 45
effective length of database: 2,649,260
effective search space: 119216700
effective search space used: 119216700
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)
Lotus: description of TM0203.8