BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0201.11
(131 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8121 UP|Q9LKJ5 (Q9LKJ5) Multifunctional transport intrinsic me... 149 2e-37
TC19136 similar to UP|AAQ11827 (AAQ11827) Nodulin-like intrinsic... 82 4e-17
TC7812 UP|Q9LKJ6 (Q9LKJ6) Water-selective transport intrinsic me... 40 1e-04
TC15862 homologue to UP|O22339 (O22339) Aquaporin-like transmemb... 40 1e-04
TC7924 homologue to UP|CAE53883 (CAE53883) Aquaporin, partial (95%) 40 1e-04
AV409092 40 1e-04
TC7806 homologue to UP|Q946J9 (Q946J9) Aquaporin protein PIP1,1,... 40 2e-04
TC8724 homologue to UP|Q9AQU5 (Q9AQU5) Plasma membrane integral ... 40 2e-04
TC7911 similar to UP|Q39800 (Q39800) Delta-tonoplast intrinsic p... 39 4e-04
TC7912 similar to UP|Q39800 (Q39800) Delta-tonoplast intrinsic p... 37 8e-04
AV428779 37 0.001
NP459447 plasma membrane intrinsic protein homolog [Lotus japoni... 37 0.001
TC7815 similar to UP|Q9AVB5 (Q9AVB5) Plasma membrane intrinsic p... 37 0.001
TC7816 homologue to UP|Q9ATM4 (Q9ATM4) Plasma membrane integral ... 36 0.002
TC14286 homologue to UP|O65357 (O65357) Aquaporin 2, complete 35 0.004
TC14103 similar to UP|Q84XC6 (Q84XC6) Plasma intrinsic protein 2... 35 0.004
TC11325 similar to UP|Q8LNX5 (Q8LNX5) Plasma membrane intrinsic ... 35 0.005
TC20270 similar to UP|O65494 (O65494) UDP-galactose transporter ... 27 1.5
AV764866 25 3.3
BP037861 25 4.4
>TC8121 UP|Q9LKJ5 (Q9LKJ5) Multifunctional transport intrinsic membrane
protein 2, complete
Length = 1213
Score = 149 bits (375), Expect = 2e-37
Identities = 72/108 (66%), Positives = 90/108 (82%), Gaps = 1/108 (0%)
Frame = +2
Query: 5 SGSNEVILNVNNETSKKCDSIEED-CVPLLQKLVAEVLGTYFLIFAGCASVVVNLNNDKV 63
+G++EV++NVN + SK + + + V LQK++AE++GTYF IFAGCAS+VVN NND V
Sbjct: 122 NGAHEVVVNVNKDASKTIEVSDTNFTVSFLQKVIAELVGTYFFIFAGCASIVVNKNNDNV 301
Query: 64 VTLPGIAIVWGLAVMVLVYSIGHISGAHFNPAVTVAHATTKSFPVKQV 111
VTLPGIA+VWGLAVMVLVYS+GHISGAHFNPA T+A A+TK FP KQV
Sbjct: 302 VTLPGIALVWGLAVMVLVYSLGHISGAHFNPAATIAFASTKRFPWKQV 445
Score = 32.0 bits (71), Expect = 0.036
Identities = 23/69 (33%), Positives = 33/69 (47%)
Frame = +2
Query: 33 LQKLVAEVLGTYFLIFAGCASVVVNLNNDKVVTLPGIAIVWGLAVMVLVYSIGHISGAHF 92
LQ V E + T+FLIF ++ + D IV G V++ V G I+GA
Sbjct: 560 LQAFVIEFIITFFLIF-----ILFGVATDDRAIGEVAGIVVGSTVLLNVLFAGPITGASM 724
Query: 93 NPAVTVAHA 101
NPA ++ A
Sbjct: 725 NPARSIGSA 751
>TC19136 similar to UP|AAQ11827 (AAQ11827) Nodulin-like intrinsic protein
NIP1-2 (Fragment), partial (29%)
Length = 556
Score = 81.6 bits (200), Expect = 4e-17
Identities = 42/82 (51%), Positives = 53/82 (64%)
Frame = +2
Query: 30 VPLLQKLVAEVLGTYFLIFAGCASVVVNLNNDKVVTLPGIAIVWGLAVMVLVYSIGHISG 89
VPL QK++AE +GT+ LIFA A +VN D L G A GL VM ++ SIGHISG
Sbjct: 308 VPLTQKILAEFVGTFILIFAATAGPIVNNKYDGAEGLMGNAATAGLTVMFIILSIGHISG 487
Query: 90 AHFNPAVTVAHATTKSFPVKQV 111
AH NP++T+A A + FP QV
Sbjct: 488 AHLNPSLTIAFAAFRHFPWSQV 553
>TC7812 UP|Q9LKJ6 (Q9LKJ6) Water-selective transport intrinsic membrane
protein 1, complete
Length = 1149
Score = 40.4 bits (93), Expect = 1e-04
Identities = 25/70 (35%), Positives = 36/70 (50%), Gaps = 5/70 (7%)
Frame = +3
Query: 33 LQKLVAEVLGTYFLIFAGCASVVV--NLNNDKVVTLPGI---AIVWGLAVMVLVYSIGHI 87
L+ +AE + T+ +FAG S + L +D T G+ +I A+ V V +I
Sbjct: 132 LRSALAEFISTFIFVFAGSGSGIAYNKLTDDGAATPAGLISASIAHAFALFVAVSISANI 311
Query: 88 SGAHFNPAVT 97
SG H NPAVT
Sbjct: 312 SGGHVNPAVT 341
>TC15862 homologue to UP|O22339 (O22339) Aquaporin-like transmembrane
channel protein, partial (61%)
Length = 576
Score = 40.4 bits (93), Expect = 1e-04
Identities = 23/64 (35%), Positives = 34/64 (52%), Gaps = 3/64 (4%)
Frame = +3
Query: 37 VAEVLGTYFLIFAGCASVVVNLNND---KVVTLPGIAIVWGLAVMVLVYSIGHISGAHFN 93
+AE + T+ ++ +V+ +D K V + GIA +G + LVY ISG H N
Sbjct: 216 IAEFVATFLFLYITILTVMGVNKSDTKCKTVGIQGIAWAFGGMIFALVYCTAGISGGHIN 395
Query: 94 PAVT 97
PAVT
Sbjct: 396 PAVT 407
>TC7924 homologue to UP|CAE53883 (CAE53883) Aquaporin, partial (95%)
Length = 1078
Score = 40.0 bits (92), Expect = 1e-04
Identities = 25/65 (38%), Positives = 34/65 (51%), Gaps = 3/65 (4%)
Frame = +2
Query: 36 LVAEVLGTYFLIFAGCASVVVNLNNDKV---VTLPGIAIVWGLAVMVLVYSIGHISGAHF 92
L+AE + T ++ A+V+ + V L GIA +G + VLVY ISG H
Sbjct: 131 LIAEFIATLLFLYVTVATVIGHKKQTGPCDGVGLLGIAWSFGGMIFVLVYCTAGISGGHI 310
Query: 93 NPAVT 97
NPAVT
Sbjct: 311 NPAVT 325
>AV409092
Length = 426
Score = 40.0 bits (92), Expect = 1e-04
Identities = 27/77 (35%), Positives = 38/77 (49%), Gaps = 5/77 (6%)
Frame = +3
Query: 30 VPLLQKLVAEVLGTYFLIFAGCASVVV--NLNNDKVVTLPG---IAIVWGLAVMVLVYSI 84
V L+ +AE + T IFAG S + L +D + G +A+ A+ V V
Sbjct: 72 VTSLKAYLAEFIATLLFIFAGVGSAIAYNELTSDAALDPTGLVAVALAHAFALFVGVSIA 251
Query: 85 GHISGAHFNPAVTVAHA 101
+ISG H NPAVT+ A
Sbjct: 252 ANISGGHLNPAVTLGLA 302
>TC7806 homologue to UP|Q946J9 (Q946J9) Aquaporin protein PIP1,1, complete
Length = 1413
Score = 39.7 bits (91), Expect = 2e-04
Identities = 23/64 (35%), Positives = 33/64 (50%), Gaps = 3/64 (4%)
Frame = +3
Query: 37 VAEVLGTYFLIFAGCASVVVNLNND---KVVTLPGIAIVWGLAVMVLVYSIGHISGAHFN 93
+AE + T+ ++ +V+ D K V + GIA +G + LVY ISG H N
Sbjct: 240 IAEFVATFLFLYITVLTVMGVSGADSKCKTVGIQGIAWAFGGMIFALVYCTAGISGGHIN 419
Query: 94 PAVT 97
PAVT
Sbjct: 420 PAVT 431
>TC8724 homologue to UP|Q9AQU5 (Q9AQU5) Plasma membrane integral protein
ZmPIP1-4 (Plasma membrane integral protein ZmPIP1-3),
partial (93%)
Length = 1560
Score = 39.7 bits (91), Expect = 2e-04
Identities = 24/64 (37%), Positives = 36/64 (55%), Gaps = 3/64 (4%)
Frame = +1
Query: 37 VAEVLGTYFLIFAGCASVV-VNLNNDKV--VTLPGIAIVWGLAVMVLVYSIGHISGAHFN 93
+AE + T+ ++ +V+ VN + +K V + GIA +G + LVY ISG H N
Sbjct: 250 IAEFVATFLFLYITVLTVMGVNRSPNKCSSVGIQGIAWAFGGMIFALVYCTAGISGGHIN 429
Query: 94 PAVT 97
PAVT
Sbjct: 430 PAVT 441
>TC7911 similar to UP|Q39800 (Q39800) Delta-tonoplast intrinsic protein,
complete
Length = 1018
Score = 38.5 bits (88), Expect = 4e-04
Identities = 24/70 (34%), Positives = 35/70 (49%), Gaps = 5/70 (7%)
Frame = +1
Query: 33 LQKLVAEVLGTYFLIFAGCASVVV--NLNNDKVVTLPG---IAIVWGLAVMVLVYSIGHI 87
++ +AE + T +FAG S + L +D + G +AI A+ V V +I
Sbjct: 109 IKAYIAEFISTLIFVFAGVGSAIAYGKLTSDAALDPAGLVAVAICHAFALFVAVSVGANI 288
Query: 88 SGAHFNPAVT 97
SG H NPAVT
Sbjct: 289 SGGHVNPAVT 318
>TC7912 similar to UP|Q39800 (Q39800) Delta-tonoplast intrinsic protein,
partial (95%)
Length = 1045
Score = 37.4 bits (85), Expect = 8e-04
Identities = 23/70 (32%), Positives = 35/70 (49%), Gaps = 5/70 (7%)
Frame = +2
Query: 33 LQKLVAEVLGTYFLIFAGCASVVV--NLNNDKVVTLPG---IAIVWGLAVMVLVYSIGHI 87
++ +AE + T +FAG S + + +D + G IA+ A+ V V +I
Sbjct: 80 IKAYIAEFISTLLFVFAGVGSAIAYGKVTSDAALDPAGLVAIAVCHAFALFVAVSVGANI 259
Query: 88 SGAHFNPAVT 97
SG H NPAVT
Sbjct: 260 SGGHVNPAVT 289
>AV428779
Length = 381
Score = 37.0 bits (84), Expect = 0.001
Identities = 23/70 (32%), Positives = 37/70 (52%), Gaps = 5/70 (7%)
Frame = +3
Query: 33 LQKLVAEVLGTYFLIFAGCASVVV--NLNNDKVVTLPGI---AIVWGLAVMVLVYSIGHI 87
L+ ++AE + T+ FAG S + L +D T G+ +I +++V V ++
Sbjct: 129 LRSVLAEFISTFIFAFAGSGSGIAYNKLTDDGAATPAGLISASIDHAFSLLVAVSISANM 308
Query: 88 SGAHFNPAVT 97
SG H NPAVT
Sbjct: 309 SGGHVNPAVT 338
>NP459447 plasma membrane intrinsic protein homolog [Lotus japonicus]
Length = 578
Score = 37.0 bits (84), Expect = 0.001
Identities = 21/64 (32%), Positives = 33/64 (50%), Gaps = 3/64 (4%)
Frame = +1
Query: 37 VAEVLGTYFLIFAGCASVVVNLNNDKV---VTLPGIAIVWGLAVMVLVYSIGHISGAHFN 93
+AE + T+ ++ +V+ + + V + GIA +G + LVY ISG H N
Sbjct: 73 IAEFIATFLFLYITVLTVMGVKRSPNMCASVGIQGIAWAFGGMIFALVYCTAGISGGHIN 252
Query: 94 PAVT 97
PAVT
Sbjct: 253 PAVT 264
>TC7815 similar to UP|Q9AVB5 (Q9AVB5) Plasma membrane intrinsic protein
2-1, complete
Length = 1450
Score = 36.6 bits (83), Expect = 0.001
Identities = 21/67 (31%), Positives = 33/67 (48%), Gaps = 5/67 (7%)
Frame = +1
Query: 36 LVAEVLGTYFLIFAGCASVVVNLNNDKV-----VTLPGIAIVWGLAVMVLVYSIGHISGA 90
L+AE + T ++ +V+ + + + GIA +G + +LVY ISG
Sbjct: 184 LIAEFIATLLFLYVTVLTVIGHSRLNSADPCDGAGILGIAWAFGGMIFILVYCTAGISGG 363
Query: 91 HFNPAVT 97
H NPAVT
Sbjct: 364 HINPAVT 384
>TC7816 homologue to UP|Q9ATM4 (Q9ATM4) Plasma membrane integral protein
ZmPIP2-7, complete
Length = 1523
Score = 35.8 bits (81), Expect = 0.002
Identities = 25/73 (34%), Positives = 34/73 (46%), Gaps = 5/73 (6%)
Frame = +1
Query: 64 VTLPGIAIVWGLAVMVLVYSIGHISGAHFNPAVTVAHATTKSFPVKQVFPLPLFKHVG-- 121
V + GIA +G + VLVY ISG H NPAVT + + + + + +G
Sbjct: 409 VGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFVGRKVSLVRALLYIIAQCLGAI 588
Query: 122 ---GSAKGFHWRF 131
G AKGF F
Sbjct: 589 CGAGLAKGFQKSF 627
>TC14286 homologue to UP|O65357 (O65357) Aquaporin 2, complete
Length = 1278
Score = 35.0 bits (79), Expect = 0.004
Identities = 17/34 (50%), Positives = 21/34 (61%)
Frame = +1
Query: 64 VTLPGIAIVWGLAVMVLVYSIGHISGAHFNPAVT 97
V + GIA +G + +LVY ISG H NPAVT
Sbjct: 337 VGILGIAWAFGGMIFILVYCTAGISGGHINPAVT 438
>TC14103 similar to UP|Q84XC6 (Q84XC6) Plasma intrinsic protein 2,2, partial
(94%)
Length = 1199
Score = 35.0 bits (79), Expect = 0.004
Identities = 17/34 (50%), Positives = 21/34 (61%)
Frame = +3
Query: 64 VTLPGIAIVWGLAVMVLVYSIGHISGAHFNPAVT 97
V + GIA +G + +LVY ISG H NPAVT
Sbjct: 321 VGILGIAWAFGGMIFILVYCTAGISGGHINPAVT 422
>TC11325 similar to UP|Q8LNX5 (Q8LNX5) Plasma membrane intrinsic protein,
partial (41%)
Length = 427
Score = 34.7 bits (78), Expect = 0.005
Identities = 20/65 (30%), Positives = 33/65 (50%), Gaps = 3/65 (4%)
Frame = +1
Query: 37 VAEVLGTYFLIFAGCASVV---VNLNNDKVVTLPGIAIVWGLAVMVLVYSIGHISGAHFN 93
+AE + T+ ++ +V+ + + V + GIA +G + LVY ISG H N
Sbjct: 223 IAEFVATFLFLYITVLTVMGVAKSKSKCSTVGVQGIAWSFGGMIFALVYCTAGISGGHIN 402
Query: 94 PAVTV 98
PA T+
Sbjct: 403 PAXTL 417
>TC20270 similar to UP|O65494 (O65494) UDP-galactose transporter - like
protein (UDP-galactose transporter-like protein),
partial (98%)
Length = 768
Score = 26.6 bits (57), Expect = 1.5
Identities = 12/37 (32%), Positives = 21/37 (56%)
Frame = +1
Query: 44 YFLIFAGCASVVVNLNNDKVVTLPGIAIVWGLAVMVL 80
+ L+ AGC + +N N+D V+ P V +A+ +L
Sbjct: 13 FILLTAGCTTAQLNSNSDHVLQTPFQGWVMAIAMALL 123
>AV764866
Length = 429
Score = 25.4 bits (54), Expect = 3.3
Identities = 13/35 (37%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Frame = -2
Query: 20 KKCDSIEEDCVPLLQKLVAEVLGTY-FLIFAGCAS 53
K C SI DC P+++ L+ L Y ++I+ AS
Sbjct: 182 KSCSSISSDCQPMIKHLLCLNLLCY*YIIYTVYAS 78
>BP037861
Length = 461
Score = 25.0 bits (53), Expect = 4.4
Identities = 14/27 (51%), Positives = 15/27 (54%)
Frame = -1
Query: 63 VVTLPGIAIVWGLAVMVLVYSIGHISG 89
VV+L G WGL M LV I ISG
Sbjct: 323 VVSLLGTPTCWGLRPM*LVIIISKISG 243
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.138 0.423
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,342,511
Number of Sequences: 28460
Number of extensions: 28176
Number of successful extensions: 147
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of query: 131
length of database: 4,897,600
effective HSP length: 80
effective length of query: 51
effective length of database: 2,620,800
effective search space: 133660800
effective search space used: 133660800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 50 (23.9 bits)
Lotus: description of TM0201.11