BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0200.6
(398 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV416299 258 1e-69
AV766719 113 5e-26
TC15163 similar to UP|Q8LGA2 (Q8LGA2) Seed maturation-like prote... 94 3e-20
TC16823 similar to UP|Q8LGA2 (Q8LGA2) Seed maturation-like prote... 91 3e-19
AV420901 75 3e-14
AV422148 52 2e-07
TC8721 36 0.010
BP051299 36 0.013
AV427932 35 0.029
TC17551 weakly similar to UP|AAR24662 (AAR24662) At5g26960, part... 34 0.037
BP072692 33 0.11
TC11910 similar to GB|CAA54498.1|602891|SCIILDNA YBL0422 {Saccha... 33 0.11
TC16172 similar to UP|Q97HY8 (Q97HY8) Uncharacterized secreted p... 33 0.11
TC8329 similar to UP|BAD06717 (BAD06717) WRKY transcription fact... 33 0.11
AV406930 32 0.14
TC11700 homologue to GB|AAL06971.1|15810091|AY056083 AT4g30260/F... 32 0.14
AV407328 32 0.24
TC13330 similar to PIR|T08400|T08400 late embryonic abundant pro... 32 0.24
AV410239 31 0.32
BI418438 31 0.32
>AV416299
Length = 403
Score = 258 bits (659), Expect = 1e-69
Identities = 133/133 (100%), Positives = 133/133 (100%)
Frame = +3
Query: 183 PMDLQGLLHDSVNVIDFGRKSNLSSKVEKVHEDVDIQDLGEISSEAQQYILNLQSRLSSM 242
PMDLQGLLHDSVNVIDFGRKSNLSSKVEKVHEDVDIQDLGEISSEAQQYILNLQSRLSSM
Sbjct: 3 PMDLQGLLHDSVNVIDFGRKSNLSSKVEKVHEDVDIQDLGEISSEAQQYILNLQSRLSSM 182
Query: 243 KKELHEVKRKNAALQMQQFVGEEKNDLLDYLRSLQPEQVAQLSEFTSPELKEIIISVVHG 302
KKELHEVKRKNAALQMQQFVGEEKNDLLDYLRSLQPEQVAQLSEFTSPELKEIIISVVHG
Sbjct: 183 KKELHEVKRKNAALQMQQFVGEEKNDLLDYLRSLQPEQVAQLSEFTSPELKEIIISVVHG 362
Query: 303 LLATLSPKMHSKP 315
LLATLSPKMHSKP
Sbjct: 363 LLATLSPKMHSKP 401
>AV766719
Length = 511
Score = 113 bits (283), Expect = 5e-26
Identities = 54/56 (96%), Positives = 55/56 (97%)
Frame = -2
Query: 343 IQFHPVISLTRDYLARLLFWCMLLGHYLRGLEYRVDLTELLSLTSDAENNGNEQIA 398
IQFHPVISLTRDYLARLLFWCMLLGHYL+GLEYRVDLTELLSLTSDAE NGNEQIA
Sbjct: 510 IQFHPVISLTRDYLARLLFWCMLLGHYLKGLEYRVDLTELLSLTSDAETNGNEQIA 343
Score = 31.2 bits (69), Expect = 0.32
Identities = 16/42 (38%), Positives = 25/42 (59%)
Frame = -2
Query: 128 QFHVVIEALWEPLSKLLISSMMTGYTLRNAEYRLCLEKNLDM 169
QFH VI + L++LL M+ G+ L+ EYR+ L + L +
Sbjct: 507 QFHPVISLTRDYLARLLFWCMLLGHYLKGLEYRVDLTELLSL 382
>TC15163 similar to UP|Q8LGA2 (Q8LGA2) Seed maturation-like protein, partial
(28%)
Length = 612
Score = 94.4 bits (233), Expect = 3e-20
Identities = 57/155 (36%), Positives = 92/155 (58%)
Frame = +2
Query: 231 YILNLQSRLSSMKKELHEVKRKNAALQMQQFVGEEKNDLLDYLRSLQPEQVAQLSEFTSP 290
YI LQS L+++K+EL+ K++ L+ + + +N+LL+YLRSL PE V +LS +S
Sbjct: 5 YIQQLQSELTNVKEELNARKQEMMQLEYDRGI---RNNLLEYLRSLDPEMVTELSRPSSV 175
Query: 291 ELKEIIISVVHGLLATLSPKMHSKPSTMSENATIGTANAGSEDCAEVVENSSIQFHPVIS 350
E+++II +V +L+ + + E + G + ++ E F +
Sbjct: 176 EVEDIIHQLVQNILSRFF--VDDASGSFMEQSVEGNIDNHPDNGDE--------FSDTVG 325
Query: 351 LTRDYLARLLFWCMLLGHYLRGLEYRVDLTELLSL 385
+RDYLA+LLFWCMLLGH+LRGLE R+ L+ ++ L
Sbjct: 326 TSRDYLAKLLFWCMLLGHHLRGLENRLQLSCVVGL 430
Score = 26.6 bits (57), Expect = 7.8
Identities = 18/61 (29%), Positives = 30/61 (48%), Gaps = 2/61 (3%)
Frame = +2
Query: 105 VPPTTADAMKRTISGMLGLLPS--DQFHVVIEALWEPLSKLLISSMMTGYTLRNAEYRLC 162
V + M++++ G + P D+F + + L+KLL M+ G+ LR E RL
Sbjct: 230 VDDASGSFMEQSVEGNIDNHPDNGDEFSDTVGTSRDYLAKLLFWCMLLGHHLRGLENRLQ 409
Query: 163 L 163
L
Sbjct: 410 L 412
>TC16823 similar to UP|Q8LGA2 (Q8LGA2) Seed maturation-like protein, partial
(29%)
Length = 711
Score = 91.3 bits (225), Expect = 3e-19
Identities = 62/165 (37%), Positives = 88/165 (52%), Gaps = 16/165 (9%)
Frame = +3
Query: 89 LIQEIEPLDVSHIQKDVPPTTADAMKRTISGMLGLLPSDQFHVVIEALWEPLSKLLISSM 148
+++ IEPLD S I K V D+MK+TIS MLGLLPSD F V + PL +LL+SS+
Sbjct: 267 MLRRIEPLDNSVISKGVSDAARDSMKQTISTMLGLLPSDHFSVTVTVSKHPLHRLLVSSI 446
Query: 149 MTGYTLRNAEYRLCLEKNLDMC------ERDLEKPKAESTPMDLQGLLHDSVNVIDFGRK 202
+TGYTL NAEYR+ L +NLD+ + D EK S ++++G D +
Sbjct: 447 ITGYTLWNAEYRMSLTRNLDIASPCGARDSDCEK---RSEILEVKGGGEDGGEI------ 599
Query: 203 SNLSSKVEKVHEDVDIQDL----------GEISSEAQQYILNLQS 237
+V D+ ++DL G++ +A YI LQS
Sbjct: 600 --------EVASDLGLKDLENCSSSPRVFGDLPPQALNYIQQLQS 710
Score = 27.7 bits (60), Expect = 3.5
Identities = 15/46 (32%), Positives = 25/46 (53%)
Frame = +3
Query: 342 SIQFHPVISLTRDYLARLLFWCMLLGHYLRGLEYRVDLTELLSLTS 387
S F +++++ L RLL ++ G+ L EYR+ LT L + S
Sbjct: 378 SDHFSVTVTVSKHPLHRLLVSSIITGYTLWNAEYRMSLTRNLDIAS 515
>AV420901
Length = 315
Score = 74.7 bits (182), Expect = 3e-14
Identities = 36/59 (61%), Positives = 46/59 (77%)
Frame = +2
Query: 111 DAMKRTISGMLGLLPSDQFHVVIEALWEPLSKLLISSMMTGYTLRNAEYRLCLEKNLDM 169
D+MK+TIS MLGLLPSD F V + PL +LL+SS++TGYTL NAEYR+ L +NLD+
Sbjct: 56 DSMKQTISTMLGLLPSDHFSVTVTVSKHPLHRLLVSSIITGYTLWNAEYRMSLTRNLDI 232
Score = 27.7 bits (60), Expect = 3.5
Identities = 15/46 (32%), Positives = 25/46 (53%)
Frame = +2
Query: 342 SIQFHPVISLTRDYLARLLFWCMLLGHYLRGLEYRVDLTELLSLTS 387
S F +++++ L RLL ++ G+ L EYR+ LT L + S
Sbjct: 101 SDHFSVTVTVSKHPLHRLLVSSIITGYTLWNAEYRMSLTRNLDIAS 238
>AV422148
Length = 460
Score = 51.6 bits (122), Expect = 2e-07
Identities = 48/184 (26%), Positives = 84/184 (45%), Gaps = 3/184 (1%)
Frame = +1
Query: 125 PSDQFHVVIEALWEPLSKLLISSMMTGYTLRNAEYRLCLEKNLDMC---ERDLEKPKAES 181
P F V I + E L++L+ S MMTGY +NA+YRL L++ L+ + +K K +
Sbjct: 1 PPQFFAVTITTVAENLAQLMYSVMMTGYMFKNAQYRLELQEGLEQVALPDVQDKKDKPDY 180
Query: 182 TPMDLQGLLHDSVNVIDFGRKSNLSSKVEKVHEDVDIQDLGEISSEAQQYILNLQSRLSS 241
P G + N+S +V + + G +A++YI
Sbjct: 181 AP----------------GTQKNVSGEVLRWNHVS-----GPERIDAKKYI--------- 270
Query: 242 MKKELHEVKRKNAALQMQQFVGEEKNDLLDYLRSLQPEQVAQLSEFTSPELKEIIISVVH 301
EL EV+ + Q+ + +N+LL+YL+SL+P + L+ ++ + + +
Sbjct: 271 ---ELLEVEIEELNRQVGRQSSNAQNELLEYLKSLEPRNLKDLTSSAGEDVVLAMNTFIK 441
Query: 302 GLLA 305
LLA
Sbjct: 442 RLLA 453
>TC8721
Length = 670
Score = 36.2 bits (82), Expect = 0.010
Identities = 17/39 (43%), Positives = 26/39 (66%)
Frame = +2
Query: 243 KKELHEVKRKNAALQMQQFVGEEKNDLLDYLRSLQPEQV 281
K +LHE R + ++Q+F+GEE+N L+DY+ S E V
Sbjct: 119 KHQLHERMRPWISKKIQEFLGEEENTLIDYIVSSTQEHV 235
>BP051299
Length = 539
Score = 35.8 bits (81), Expect = 0.013
Identities = 27/85 (31%), Positives = 36/85 (41%), Gaps = 2/85 (2%)
Frame = +3
Query: 28 PPFPSSPLFLFLFSTSALHPPHPSLSLLSATTQTLQNRNPLSWFLL--PQPPPSGISPPN 85
PP PSSP ST+ HPP S S ++ + + P + L P PPPS PP+
Sbjct: 102 PPPPSSP------STATTHPPPSSSSSATSVLFPISSTTPSAATTLFSPNPPPSASPPPS 263
Query: 86 PRELIQEIEPLDVSHIQKDVPPTTA 110
+ V+ PP TA
Sbjct: 264 ---TVNSPSSESVTASSASPPPKTA 329
>AV427932
Length = 425
Score = 34.7 bits (78), Expect = 0.029
Identities = 17/53 (32%), Positives = 29/53 (54%), Gaps = 4/53 (7%)
Frame = +1
Query: 77 PPSGISPPNPRELIQE----IEPLDVSHIQKDVPPTTADAMKRTISGMLGLLP 125
PP+G + RE++ E ++P + K P DAM++T++ M+G LP
Sbjct: 265 PPNGTLSKSRREILLEYVKNVQPEFMEMFVKRAPQQVVDAMRQTVTNMIGTLP 423
>TC17551 weakly similar to UP|AAR24662 (AAR24662) At5g26960, partial (22%)
Length = 505
Score = 34.3 bits (77), Expect = 0.037
Identities = 25/74 (33%), Positives = 31/74 (41%), Gaps = 15/74 (20%)
Frame = +3
Query: 28 PPFPSSPLFLFLFSTSALHP----PHPSLSLLSATTQT-----------LQNRNPLSWFL 72
PPFP S F S+ A HP P PS + + T+ T + P S
Sbjct: 123 PPFPPSLTTSFSTSSPASHPLPSLPSPSSAAVGPTSSTPPTSPTSAAAATSSATPPS--- 293
Query: 73 LPQPPPSGISPPNP 86
P PPP+ SPP P
Sbjct: 294 -PSPPPTSASPPPP 332
>BP072692
Length = 422
Score = 32.7 bits (73), Expect = 0.11
Identities = 19/57 (33%), Positives = 28/57 (48%)
Frame = +3
Query: 30 FPSSPLFLFLFSTSALHPPHPSLSLLSATTQTLQNRNPLSWFLLPQPPPSGISPPNP 86
F + +FL F A H P SL+++S +T R + LP+ PP + PP P
Sbjct: 78 FRRASIFLIFFHVHAFHDPPNSLTMVSDSTL---RRGLIVGN*LPRRPPPRLPPPRP 239
>TC11910 similar to GB|CAA54498.1|602891|SCIILDNA YBL0422 {Saccharomyces
cerevisiae;} , partial (6%)
Length = 638
Score = 32.7 bits (73), Expect = 0.11
Identities = 17/45 (37%), Positives = 23/45 (50%)
Frame = -1
Query: 47 PPHPSLSLLSATTQTLQNRNPLSWFLLPQPPPSGISPPNPRELIQ 91
PPH S+ TLQ+ NP+S F+LP + PP P + Q
Sbjct: 491 PPHFLQSINPEPLHTLQSINPISCFILPSLQKTHFIPPLPAQRSQ 357
>TC16172 similar to UP|Q97HY8 (Q97HY8) Uncharacterized secreted protein,
homolog YXKC Bacillus subtilis, partial (6%)
Length = 591
Score = 32.7 bits (73), Expect = 0.11
Identities = 21/57 (36%), Positives = 28/57 (48%)
Frame = +3
Query: 125 PSDQFHVVIEALWEPLSKLLISSMMTGYTLRNAEYRLCLEKNLDMCERDLEKPKAES 181
PS V E LSKLL M+ GY++RN E R +E+ L + E P E+
Sbjct: 15 PSQMKTSVTETSATELSKLLYWLMVVGYSIRNIEVRYDMERVLGTPPKLAELPPGEN 185
>TC8329 similar to UP|BAD06717 (BAD06717) WRKY transcription factor 1,
partial (41%)
Length = 1717
Score = 32.7 bits (73), Expect = 0.11
Identities = 21/57 (36%), Positives = 26/57 (44%)
Frame = -2
Query: 23 CSSWQPPFPSSPLFLFLFSTSALHPPHPSLSLLSATTQTLQNRNPLSWFLLPQPPPS 79
CSS P P+F FL S ++ PP L+LL TQ+ R PPPS
Sbjct: 333 CSSHTPLRAFQPIFCFLLSPASAPPPTHWLTLLQQKTQSSPKRKACF------PPPS 181
>AV406930
Length = 433
Score = 32.3 bits (72), Expect = 0.14
Identities = 18/48 (37%), Positives = 22/48 (45%)
Frame = +1
Query: 46 HPPHPSLSLLSATTQTLQNRNPLSWFLLPQPPPSGISPPNPRELIQEI 93
HPPH S+S L+ FL P PPP PPNP + Q +
Sbjct: 31 HPPHSSISALALAPPH---------FLAPPPPPQ---PPNPPSISQNL 138
Score = 28.1 bits (61), Expect = 2.7
Identities = 21/58 (36%), Positives = 23/58 (39%)
Frame = +1
Query: 27 QPPFPSSPLFLFLFSTSALHPPHPSLSLLSATTQTLQNRNPLSWFLLPQPPPSGISPP 84
QPP P S ST + P H LS PLS+ LP P PS S P
Sbjct: 103 QPPNPPSISQNLSISTHPITPFHTPLSHSFNLPLPKPASIPLSFSALPSPSPSPSSSP 276
>TC11700 homologue to GB|AAL06971.1|15810091|AY056083 AT4g30260/F9N11_110
{Arabidopsis thaliana;}, partial (45%)
Length = 561
Score = 32.3 bits (72), Expect = 0.14
Identities = 23/61 (37%), Positives = 27/61 (43%), Gaps = 4/61 (6%)
Frame = +3
Query: 28 PPFPSSPLFLFLFSTSAL----HPPHPSLSLLSATTQTLQNRNPLSWFLLPQPPPSGISP 83
PP PS PL TS L +PP P+L+ +NP LL PPP SP
Sbjct: 156 PPSPSPPLLPSSIPTSLLLFLPNPPSPNLNF----------QNPSLSLLLLLPPPDPKSP 305
Query: 84 P 84
P
Sbjct: 306 P 308
>AV407328
Length = 428
Score = 31.6 bits (70), Expect = 0.24
Identities = 18/41 (43%), Positives = 21/41 (50%)
Frame = +3
Query: 39 LFSTSALHPPHPSLSLLSATTQTLQNRNPLSWFLLPQPPPS 79
L +S H P PSL LL + L+ NPL LL PPS
Sbjct: 9 LLPSSTTHLPSPSLLLLLPASSNLEPFNPLLRSLLNPKPPS 131
>TC13330 similar to PIR|T08400|T08400 late embryonic abundant protein EMB8
homolog F18B3.70 - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (21%)
Length = 434
Score = 31.6 bits (70), Expect = 0.24
Identities = 29/95 (30%), Positives = 41/95 (42%), Gaps = 3/95 (3%)
Frame = +1
Query: 17 SGCIGGCSSWQPPFPSSPLFLFLFSTSALH---PPHPSLSLLSATTQTLQNRNPLSWFLL 73
S I C++ PP SS FLFL H P P L T +PL L
Sbjct: 4 SSSIQQCTNQIPPLVSSSFFLFLRRNHVHHHHPTPTPLLRNPRRRT*PFPPYSPLLGSSL 183
Query: 74 PQPPPSGISPPNPRELIQEIEPLDVSHIQKDVPPT 108
P P + PP+ R+ ++ + PL H ++ PP+
Sbjct: 184 PPFPLPCLEPPH-RDHLRLLLPL---HPRRQAPPS 276
>AV410239
Length = 432
Score = 31.2 bits (69), Expect = 0.32
Identities = 22/64 (34%), Positives = 30/64 (46%), Gaps = 5/64 (7%)
Frame = +1
Query: 31 PSSPLFLFLFSTSALHPPHPSLSLLSATTQTLQNRN-----PLSWFLLPQPPPSGISPPN 85
P P+ L ST+ LHPP + SAT L R+ PL +P+ + SPP
Sbjct: 28 PWLPILLLRVSTTVLHPPTIPIPAQSATPFPLPARDAAAAPPLHQRPVPRRTTAPDSPPM 207
Query: 86 PREL 89
P +L
Sbjct: 208PPQL 219
>BI418438
Length = 431
Score = 31.2 bits (69), Expect = 0.32
Identities = 26/95 (27%), Positives = 37/95 (38%), Gaps = 1/95 (1%)
Frame = +1
Query: 25 SWQPPFPSSPLFL-FLFSTSALHPPHPSLSLLSATTQTLQNRNPLSWFLLPQPPPSGISP 83
S PPF PL LFS+S+ P P + + TT +P PPPS P
Sbjct: 130 SQYPPFSPCPLSSPSLFSSSSSPSPPPPTATTTTTT-------------IPPPPPSPTPP 270
Query: 84 PNPRELIQEIEPLDVSHIQKDVPPTTADAMKRTIS 118
P + P S+ PP+ + + +S
Sbjct: 271 PRQKSSKPARPPASPSNAN---PPSPTSLLTQPLS 366
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.315 0.132 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,351,473
Number of Sequences: 28460
Number of extensions: 136305
Number of successful extensions: 1179
Number of sequences better than 10.0: 114
Number of HSP's better than 10.0 without gapping: 1088
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1153
length of query: 398
length of database: 4,897,600
effective HSP length: 92
effective length of query: 306
effective length of database: 2,279,280
effective search space: 697459680
effective search space used: 697459680
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0200.6