BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0195a.3
(660 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP052151 38 0.005
BP052159 36 0.023
TC14309 homologue to UP|H2B_GOSHI (O22582) Histone H2B, partial ... 35 0.038
TC15802 similar to UP|Q9XIV1 (Q9XIV1) mRNA expressed in cucumber... 34 0.086
TC15534 weakly similar to GB|AAO63420.1|28950993|BT005356 At5g28... 33 0.19
TC8142 UP|BAD11133 (BAD11133) Glutamate-rich protein, complete 32 0.43
AV774953 31 0.72
TC14162 similar to UP|OLP1_LYCES (Q41350) Osmotin-like protein p... 31 0.72
TC11009 similar to GB|AAP21377.1|30102918|BT006569 At1g47970 {Ar... 30 1.2
TC19870 30 1.2
TC8106 weakly similar to UP|Q9XIV1 (Q9XIV1) mRNA expressed in cu... 30 1.6
TC15397 similar to UP|CALX_SOYBN (Q39817) Calnexin homolog precu... 29 2.8
AV418552 29 2.8
TC14264 similar to UP|Q84NF9 (Q84NF9) Histone H1 (Fragment), par... 28 3.6
TC18216 similar to GB|CAA28991.1|34041|HSKER65A keratin type II ... 28 4.7
TC16579 weakly similar to UP|AAR82740 (AAR82740) SD09672p (Fragm... 28 4.7
TC14634 similar to UP|Q7X998 (Q7X998) mRNA-binding protein (Frag... 28 6.1
TC12321 similar to UP|Q15415 (Q15415) YRRM2, partial (5%) 27 8.0
>BP052151
Length = 495
Score = 38.1 bits (87), Expect = 0.005
Identities = 15/25 (60%), Positives = 20/25 (80%)
Frame = -1
Query: 623 SSYLWKTQKILEQLELDNYFAVFRS 647
SSYLW T KIL++LE+D Y+ V+ S
Sbjct: 495 SSYLWATPKILDELEMDTYYPVYNS 421
>BP052159
Length = 452
Score = 35.8 bits (81), Expect = 0.023
Identities = 13/23 (56%), Positives = 19/23 (82%)
Frame = -1
Query: 625 YLWKTQKILEQLELDNYFAVFRS 647
YLW T+KIL++LE+D Y+ V+ S
Sbjct: 452 YLWATRKILDELEMDTYYPVYNS 384
>TC14309 homologue to UP|H2B_GOSHI (O22582) Histone H2B, partial (93%)
Length = 682
Score = 35.0 bits (79), Expect = 0.038
Identities = 22/47 (46%), Positives = 25/47 (52%), Gaps = 3/47 (6%)
Frame = -3
Query: 325 ALAPTFGSIVP---FIGAGGFAAAATATGSVAGSVAVAASFGAAGAG 368
ALAP+FGS P F AGG +AA S AG + A F AA G
Sbjct: 203 ALAPSFGSFFPALGFFSAGGAFSAAVDFFSSAGFFSAAGFFSAAALG 63
>TC15802 similar to UP|Q9XIV1 (Q9XIV1) mRNA expressed in cucumber hypocotyls
(Arabinogalactan protein), partial (26%)
Length = 795
Score = 33.9 bits (76), Expect = 0.086
Identities = 34/97 (35%), Positives = 40/97 (41%), Gaps = 10/97 (10%)
Frame = -1
Query: 296 IIGAAAVTGGTLMAITGGLAAPAIAHGLGALAPTFGSIVPFIGAGGFAAAATATGS---- 351
++GA A T G +TG AA A G GA G + AGG A TA G
Sbjct: 441 LLGAGAGTSGV---VTG--AATGTAVGAGAGGDLAGGELTGTAAGGGEATGTAAGGGEVT 277
Query: 352 ------VAGSVAVAASFGAAGAGLTGSKMATRIGSLE 382
VAG+ AVA AAG G AT +E
Sbjct: 276 GVTGVVVAGAGAVAVEGDAAGVVAAGLGEATGATGVE 166
>TC15534 weakly similar to GB|AAO63420.1|28950993|BT005356 At5g28040
{Arabidopsis thaliana;}, partial (8%)
Length = 937
Score = 32.7 bits (73), Expect = 0.19
Identities = 34/105 (32%), Positives = 43/105 (40%), Gaps = 10/105 (9%)
Frame = -3
Query: 100 SDANSSERLDKEAALTKAVDAMTESMSTASIAESSSGESGGHEQKSQDYLC--------- 150
S + S E D+ + K A E E SS E G E++ +D
Sbjct: 836 SSSESEEEDDQPLSQVKHAAAEEE--------ELSSEEEGSSEEEEEDETTAPAPPAATT 681
Query: 151 NTTAPPVVEPHSDSKTK-DSESSALLPQQKQASTELENVTLEKPL 194
+ T PP EP SDS T+ DSES Q AS N + KPL
Sbjct: 680 SHTKPPQPEPESDSATQSDSESDTDSDQAPSASAPTPNPKV-KPL 549
>TC8142 UP|BAD11133 (BAD11133) Glutamate-rich protein, complete
Length = 999
Score = 31.6 bits (70), Expect = 0.43
Identities = 22/78 (28%), Positives = 39/78 (49%)
Frame = -2
Query: 303 TGGTLMAITGGLAAPAIAHGLGALAPTFGSIVPFIGAGGFAAAATATGSVAGSVAVAASF 362
+G + TG + +++ G ++ +FGS V AG A +A G + +VAA
Sbjct: 350 SGSWVSGATGASVSVSVSFG-SSVVSSFGSSV----AGSAGAGTSAAGCSGAATSVAA-- 192
Query: 363 GAAGAGLTGSKMATRIGS 380
G +G+G+ + T +GS
Sbjct: 191 GCSGSGIVS*ILVTSVGS 138
>AV774953
Length = 579
Score = 30.8 bits (68), Expect = 0.72
Identities = 26/83 (31%), Positives = 39/83 (46%), Gaps = 2/83 (2%)
Frame = +1
Query: 126 STASIAESSSGESGGHEQKSQDYLCNTTAPPVVEPHSDSKTKDSESSALLPQQKQASTEL 185
S+ S +++ ES E K++ TA P D D+E + KQ++ E
Sbjct: 331 SSGSEKDNTETESASEEGKAESAAEENTADP--NDADDKPETDAEET------KQSNVEG 486
Query: 186 ENVTLEKPL--EEASLISYQRKV 206
+NVT +KP EEASL K+
Sbjct: 487 DNVTAKKPQGEEEASLAESGEKL 555
>TC14162 similar to UP|OLP1_LYCES (Q41350) Osmotin-like protein precursor,
partial (87%)
Length = 1232
Score = 30.8 bits (68), Expect = 0.72
Identities = 34/95 (35%), Positives = 38/95 (39%), Gaps = 15/95 (15%)
Frame = -3
Query: 289 DKWKRGGIIGAAAVTGGTLMAIT-----GGLAAPAIAHGLGALAPTFGSIVPFI-----G 338
D+W RG IGAA A G + A A G+G G V + G
Sbjct: 540 DRWTRGEQIGAATDNRANTFAFVRGYSHGDVEAVDEADGVGG---EVGVAVVHVELG*GG 370
Query: 339 AGGFAAA-----ATATGSVAGSVAVAASFGAAGAG 368
GG A A A A AG AVA GAAGAG
Sbjct: 369 WGGTAGAGALETAAAVAGCAGE-AVAGGVGAAGAG 268
>TC11009 similar to GB|AAP21377.1|30102918|BT006569 At1g47970 {Arabidopsis
thaliana;}, partial (44%)
Length = 700
Score = 30.0 bits (66), Expect = 1.2
Identities = 21/77 (27%), Positives = 29/77 (37%)
Frame = -2
Query: 96 LAEESDANSSERLDKEAALTKAVDAMTESMSTASIAESSSGESGGHEQKSQDYLCNTTAP 155
L+ S ++ S+ LD ++ A T S + SSS S S P
Sbjct: 330 LSSPSSSSGSKSLDASSSSAAATVRRTRYSVPKSSSSSSSSSSSSSSSSSSSGSPPPPRP 151
Query: 156 PVVEPHSDSKTKDSESS 172
P P S S + S SS
Sbjct: 150 PSTPPSSSSSSSSSSSS 100
>TC19870
Length = 481
Score = 30.0 bits (66), Expect = 1.2
Identities = 20/51 (39%), Positives = 28/51 (54%), Gaps = 4/51 (7%)
Frame = +2
Query: 572 AGRF--VNAYSTNDWTLGIT--FRASLLSQGLAGIQPVDVPGIENVDVTQL 618
A RF +++ S + W + + SL QGLAGI P+ PGI VD +L
Sbjct: 104 ASRFTAISSSSKSLWGASLKKFLKLSLHFQGLAGIHPIHFPGIPIVDRDRL 256
Score = 28.9 bits (63), Expect = 2.8
Identities = 16/37 (43%), Positives = 23/37 (61%)
Frame = +3
Query: 609 GIENVDVTQLIEGHSSYLWKTQKILEQLELDNYFAVF 645
G + + VT +EG SY+ T IL+ LELD Y+ V+
Sbjct: 228 GSQLLTVTD*VEGRFSYI*ATL-ILDPLELDKYYPVY 335
>TC8106 weakly similar to UP|Q9XIV1 (Q9XIV1) mRNA expressed in cucumber
hypocotyls (Arabinogalactan protein), partial (32%)
Length = 760
Score = 29.6 bits (65), Expect = 1.6
Identities = 25/76 (32%), Positives = 30/76 (38%)
Frame = -3
Query: 295 GIIGAAAVTGGTLMAITGGLAAPAIAHGLGALAPTFGSIVPFIGAGGFAAAATATGSVAG 354
G++GA A GG L G A G G L T AGG A A ATG+ A
Sbjct: 212 GVVGAGA--GGELTGTGAGGEATGTGAGGGELTGT--------AAGGVDAGALATGAGAA 63
Query: 355 SVAVAASFGAAGAGLT 370
+ A G+T
Sbjct: 62 AALDGGEARGADTGVT 15
Score = 29.3 bits (64), Expect = 2.1
Identities = 26/79 (32%), Positives = 30/79 (37%), Gaps = 15/79 (18%)
Frame = -3
Query: 309 AITGGLAAPAIAHGLGA--LAPTFGSIVPFIGAG------GFAAAATATGSVAGSVAV-- 358
A TGG P+ G GA P F +GAG G A A G+ AG
Sbjct: 350 APTGGAGGPSNGEGAGAPCFFPFFTFFFDLLGAGAGTSAVGVTAGAGVVGAGAGGELTGT 171
Query: 359 -----AASFGAAGAGLTGS 372
A GA G LTG+
Sbjct: 170 GAGGEATGTGAGGGELTGT 114
>TC15397 similar to UP|CALX_SOYBN (Q39817) Calnexin homolog precursor,
partial (58%)
Length = 1103
Score = 28.9 bits (63), Expect = 2.8
Identities = 14/23 (60%), Positives = 15/23 (64%), Gaps = 4/23 (17%)
Frame = -2
Query: 47 PELWIHD----NFGLLSPVLRFL 65
P LW+H NFG L PVLRFL
Sbjct: 877 PVLWLHSIWVGNFGSLIPVLRFL 809
>AV418552
Length = 386
Score = 28.9 bits (63), Expect = 2.8
Identities = 26/87 (29%), Positives = 39/87 (43%), Gaps = 4/87 (4%)
Frame = -1
Query: 299 AAAVTGGTLMAITGGLAAPAIAHGLGALAPTFGSIVPFIGAGGFAAAATATG----SVAG 354
AA V G + +AA + G+ + +V +GA AAA G +V G
Sbjct: 326 AARVVAGVVCVDVV*VAAAPVVVGVVQVVVVGAVLVAAVGAV*VAAARVVVGAVQVAVVG 147
Query: 355 SVAVAASFGAAGAGLTGSKMATRIGSL 381
+ AVAAS A AG+ M +G++
Sbjct: 146 AAAVAASEEEAAAGV--ENMIASVGAV 72
>TC14264 similar to UP|Q84NF9 (Q84NF9) Histone H1 (Fragment), partial (76%)
Length = 1277
Score = 28.5 bits (62), Expect = 3.6
Identities = 26/89 (29%), Positives = 34/89 (37%), Gaps = 2/89 (2%)
Frame = -3
Query: 293 RGGIIGAAAVTG--GTLMAITGGLAAPAIAHGLGALAPTFGSIVPFIGAGGFAAAATATG 350
RG ++ A G G A+ G A A A GL A GFA A
Sbjct: 777 RGAVLDFAFALGLAGEAFALAGAATALAFAAGLAL-------------AAGFALGLAAAA 637
Query: 351 SVAGSVAVAASFGAAGAGLTGSKMATRIG 379
A +V + A+ GAGL + +A G
Sbjct: 636 GFALAVVLVAAAFDLGAGLALALVAAAAG 550
Score = 27.7 bits (60), Expect = 6.1
Identities = 34/107 (31%), Positives = 45/107 (41%), Gaps = 17/107 (15%)
Frame = -3
Query: 295 GIIGAAAVTGG--TLMAITGGLA-APAIAHGLGALAPTFGSIVPF-----IGAGGFAAAA 346
G+ G A G T +A GLA A A GL A A ++V +GAG A
Sbjct: 744 GLAGEAFALAGAATALAFAAGLALAAGFALGLAAAAGFALAVVLVAAAFDLGAGLALALV 565
Query: 347 TATGSVAGSVAVAASFGAAG-------AGLTGSKMATRI--GSLEEF 384
A +A ++ + AAG AGL + A GSL+EF
Sbjct: 564 AAAAGLALALGLGLDLAAAGCGFFLAAAGLEAAAAAEDFADGSLKEF 424
>TC18216 similar to GB|CAA28991.1|34041|HSKER65A keratin type II (AA1-215)
{Homo sapiens;} , partial (16%)
Length = 501
Score = 28.1 bits (61), Expect = 4.7
Identities = 22/49 (44%), Positives = 24/49 (48%), Gaps = 5/49 (10%)
Frame = +2
Query: 325 ALAPT---FGSIVPFIGAGGFAAAATATGSVAGSVAV--AASFGAAGAG 368
A APT FG G G AAA T+ G G AV A FG AG+G
Sbjct: 2 AAAPTSGGFGGAASTGGGFGAAAAPTSGGFGGGFGAVPSAGGFGGAGSG 148
>TC16579 weakly similar to UP|AAR82740 (AAR82740) SD09672p (Fragment),
partial (5%)
Length = 705
Score = 28.1 bits (61), Expect = 4.7
Identities = 23/65 (35%), Positives = 37/65 (56%), Gaps = 4/65 (6%)
Frame = +3
Query: 213 VSACVADTAEVDNKCCRS-RQGYDA-RHRVS--LRLIATWLGVKWNEMEAMESMVAFSLM 268
V+ VAD A+ D + Q +D R +V+ L+ IA+ G W+E + ++SM++F L
Sbjct: 51 VAVAVADDADADASNHKDVEQKFDVPREQVTQILQAIAS-TGKFWHEWDNLKSMLSFQLK 227
Query: 269 NSLSE 273
LSE
Sbjct: 228 QVLSE 242
>TC14634 similar to UP|Q7X998 (Q7X998) mRNA-binding protein (Fragment),
partial (43%)
Length = 799
Score = 27.7 bits (60), Expect = 6.1
Identities = 15/41 (36%), Positives = 21/41 (50%)
Frame = +2
Query: 4 TPSMLLPPTHRYAAGALFALALHHSQIHQSPTTAQNAASVS 44
T S LLP +H F+ + H S + SPT + +SVS
Sbjct: 110 TSSKLLPLSHSSTLRLSFSSSFHSSSLSVSPTFLAHPSSVS 232
>TC12321 similar to UP|Q15415 (Q15415) YRRM2, partial (5%)
Length = 812
Score = 27.3 bits (59), Expect = 8.0
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = +1
Query: 152 TTAPPVVEPHSDSKTKDSESSALLPQQKQASTE 184
TT PP PHS SK K +++ P A+++
Sbjct: 505 TTLPPTQSPHSRSKPKLKPAASTSPTSSSAASK 603
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.314 0.129 0.368
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,900,935
Number of Sequences: 28460
Number of extensions: 117974
Number of successful extensions: 588
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 575
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 584
length of query: 660
length of database: 4,897,600
effective HSP length: 96
effective length of query: 564
effective length of database: 2,165,440
effective search space: 1221308160
effective search space used: 1221308160
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0195a.3