BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0191.7
(680 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP036228 416 e-117
TC14669 similar to PIR|E86326|E86326 protein F18O14.3 [imported]... 44 7e-05
TC11793 similar to UP|AAR99376 (AAR99376) Ring domain containing... 43 2e-04
AV411356 42 3e-04
TC9277 similar to GB|AAP13413.1|30023760|BT006305 At3g55530 {Ara... 41 6e-04
TC19557 similar to PIR|E86326|E86326 protein F18O14.3 [imported]... 41 6e-04
TC18788 weakly similar to UP|Q9SSA6 (Q9SSA6) F4P13.1 protein, pa... 40 0.002
TC12104 40 0.002
TC12042 similar to GB|CAB87983.1|7576235|ATH276134 Pex10p {Arabi... 39 0.002
TC15777 similar to UP|AAR99376 (AAR99376) Ring domain containing... 39 0.002
TC19774 weakly similar to UP|AAR92268 (AAR92268) At1g47570, part... 39 0.004
BP045070 38 0.005
TC10567 similar to UP|AAR99376 (AAR99376) Ring domain containing... 38 0.006
AV406621 37 0.008
TC14940 37 0.010
BP045442 36 0.023
AV406818 35 0.030
TC13904 weakly similar to UP|Q9ZU51 (Q9ZU51) RING-H2 finger prot... 34 0.068
TC11949 34 0.089
BP084627 34 0.089
>BP036228
Length = 626
Score = 416 bits (1069), Expect = e-117
Identities = 193/193 (100%), Positives = 193/193 (100%)
Frame = +2
Query: 1 MAHHLPCDADGTCMLCKLKPPPSETLQCHTCITPWHLPCLPTPPASFVNWDCPDCSSSIS 60
MAHHLPCDADGTCMLCKLKPPPSETLQCHTCITPWHLPCLPTPPASFVNWDCPDCSSSIS
Sbjct: 47 MAHHLPCDADGTCMLCKLKPPPSETLQCHTCITPWHLPCLPTPPASFVNWDCPDCSSSIS 226
Query: 61 DFHAPAPAPSAGADLVSAIRAIDSDASLTDQQKAKKKQELVGGPSSGEKPPAAGGILDIF 120
DFHAPAPAPSAGADLVSAIRAIDSDASLTDQQKAKKKQELVGGPSSGEKPPAAGGILDIF
Sbjct: 227 DFHAPAPAPSAGADLVSAIRAIDSDASLTDQQKAKKKQELVGGPSSGEKPPAAGGILDIF 406
Query: 121 DGSINCSFCMQLPDRPVTTPCGHNFCLKCFEKWIGQGKRTCANCRRDIPAKIASQPRINS 180
DGSINCSFCMQLPDRPVTTPCGHNFCLKCFEKWIGQGKRTCANCRRDIPAKIASQPRINS
Sbjct: 407 DGSINCSFCMQLPDRPVTTPCGHNFCLKCFEKWIGQGKRTCANCRRDIPAKIASQPRINS 586
Query: 181 QLAIAIRLAKLAK 193
QLAIAIRLAKLAK
Sbjct: 587 QLAIAIRLAKLAK 625
Score = 40.8 bits (94), Expect = 7e-04
Identities = 23/76 (30%), Positives = 34/76 (44%), Gaps = 1/76 (1%)
Frame = +2
Query: 494 CNICHKVLSSPLTTPCAHNFCKVCLEGAFSGKSFIRKRACEGGRSLRAQKNVMKCPSCSN 553
C+ C ++ P+TTPC HNFC C E K +G R+ C +C
Sbjct: 422 CSFCMQLPDRPVTTPCGHNFCLKCFE----------KWIGQGKRT---------CANCRR 544
Query: 554 DI-ADFLQNPQVNREM 568
DI A P++N ++
Sbjct: 545 DIPAKIASQPRINSQL 592
>TC14669 similar to PIR|E86326|E86326 protein F18O14.3 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(77%)
Length = 1120
Score = 44.3 bits (103), Expect = 7e-05
Identities = 22/67 (32%), Positives = 30/67 (43%), Gaps = 4/67 (5%)
Frame = +2
Query: 103 GPSSGEKPPAAG--GILDIFDGSINCSFCMQLPDRPVTTPCGHNFCLKCFEKWI--GQGK 158
G S+ PP+ G + G+ C+ C L P+ T CGH FC C KW+
Sbjct: 53 GESTSRSPPSPSYAGNNNSDAGNFECNICFDLAQDPIITLCGHLFCWPCLYKWLHFHSQS 232
Query: 159 RTCANCR 165
R C C+
Sbjct: 233 RECPVCK 253
Score = 34.3 bits (77), Expect = 0.068
Identities = 13/27 (48%), Positives = 15/27 (55%)
Frame = +2
Query: 492 FGCNICHKVLSSPLTTPCAHNFCKVCL 518
F CNIC + P+ T C H FC CL
Sbjct: 122 FECNICFDLAQDPIITLCGHLFCWPCL 202
>TC11793 similar to UP|AAR99376 (AAR99376) Ring domain containing protein,
partial (23%)
Length = 527
Score = 42.7 bits (99), Expect = 2e-04
Identities = 15/48 (31%), Positives = 23/48 (47%)
Frame = +3
Query: 122 GSINCSFCMQLPDRPVTTPCGHNFCLKCFEKWIGQGKRTCANCRRDIP 169
G +C+ C+ PV T CGH +C C KW+ + N ++ P
Sbjct: 339 GGFDCNICLDCVQDPVVTLCGHLYCWPCIYKWLNSNSASSENVEQNKP 482
Score = 37.4 bits (85), Expect = 0.008
Identities = 17/65 (26%), Positives = 29/65 (44%)
Frame = +3
Query: 492 FGCNICHKVLSSPLTTPCAHNFCKVCLEGAFSGKSFIRKRACEGGRSLRAQKNVMKCPSC 551
F CNIC + P+ T C H +C C+ + S S ++N +CP C
Sbjct: 345 FDCNICLDCVQDPVVTLCGHLYCWPCIYKWLNSNS---------ASSENVEQNKPQCPVC 497
Query: 552 SNDIA 556
++++
Sbjct: 498 KSEVS 512
>AV411356
Length = 358
Score = 42.0 bits (97), Expect = 3e-04
Identities = 19/56 (33%), Positives = 27/56 (47%), Gaps = 2/56 (3%)
Frame = +2
Query: 118 DIFDGSINCSFCMQLPDRPVTTPCGHNFCLKCFEKW--IGQGKRTCANCRRDIPAK 171
D FD C+ C+ L PV T CGH FC C +W + + C C+ ++ K
Sbjct: 32 DFFD----CNICLDLAREPVVTCCGHLFCWTCVYRWLHLHSDAKECPVCKGEVTLK 187
Score = 34.7 bits (78), Expect = 0.052
Identities = 17/64 (26%), Positives = 24/64 (36%)
Frame = +2
Query: 492 FGCNICHKVLSSPLTTPCAHNFCKVCLEGAFSGKSFIRKRACEGGRSLRAQKNVMKCPSC 551
F CNIC + P+ T C H FC C+ R L + +CP C
Sbjct: 38 FDCNICLDLAREPVVTCCGHLFCWTCVY-----------------RWLHLHSDAKECPVC 166
Query: 552 SNDI 555
++
Sbjct: 167 KGEV 178
>TC9277 similar to GB|AAP13413.1|30023760|BT006305 At3g55530 {Arabidopsis
thaliana;}, partial (79%)
Length = 1297
Score = 41.2 bits (95), Expect = 6e-04
Identities = 31/112 (27%), Positives = 49/112 (43%), Gaps = 25/112 (22%)
Frame = +3
Query: 79 IRAIDSD-----ASLTDQQ--KAKKKQELVGGPSSGEKPPAAG---------------GI 116
+RA+DSD S+T+++ + V GPSSG P A G
Sbjct: 675 LRALDSDNVSTAPSMTEEEINALPVHKYKVSGPSSGSSLPQASPSTSAEKEQDKSNAVGS 854
Query: 117 LDIFDGSINCSFCMQ---LPDRPVTTPCGHNFCLKCFEKWIGQGKRTCANCR 165
+ + D + CS C++ + D + PC H F C + W+ Q + TC C+
Sbjct: 855 MKVSDDELTCSVCLEQVNVGDILRSLPCLHQFHANCIDPWLRQ-QGTCPVCK 1007
>TC19557 similar to PIR|E86326|E86326 protein F18O14.3 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(24%)
Length = 457
Score = 41.2 bits (95), Expect = 6e-04
Identities = 16/46 (34%), Positives = 23/46 (49%), Gaps = 2/46 (4%)
Frame = +2
Query: 122 GSINCSFCMQLPDRPVTTPCGHNFCLKCFEKWIGQGKRT--CANCR 165
G C+ C +L PV T CGH FC C +W+ ++ C C+
Sbjct: 299 GDFECNICFELAQDPVITLCGHLFCWPCLYRWLHHHSQSQECPVCK 436
Score = 35.4 bits (80), Expect = 0.030
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +2
Query: 491 EFGCNICHKVLSSPLTTPCAHNFCKVCL 518
+F CNIC ++ P+ T C H FC CL
Sbjct: 302 DFECNICFELAQDPVITLCGHLFCWPCL 385
>TC18788 weakly similar to UP|Q9SSA6 (Q9SSA6) F4P13.1 protein, partial (7%)
Length = 951
Score = 39.7 bits (91), Expect = 0.002
Identities = 18/48 (37%), Positives = 21/48 (43%), Gaps = 2/48 (4%)
Frame = +1
Query: 10 DGTCMLCKLKPPPSETLQCHTCITPWHLPCLPTPPASFV--NWDCPDC 55
+G C +C L L C TC +H CL P A NW CP C
Sbjct: 106 EGVCKVCGLDKDDDSVLLCDTCDAEYHTYCLNPPLARIPEGNWYCPSC 249
>TC12104
Length = 771
Score = 39.7 bits (91), Expect = 0.002
Identities = 23/77 (29%), Positives = 36/77 (45%), Gaps = 1/77 (1%)
Frame = +1
Query: 93 KAKKKQEL-VGGPSSGEKPPAAGGILDIFDGSINCSFCMQLPDRPVTTPCGHNFCLKCFE 151
KA K E+ G ++ E+ AAG + C+ C + P+ C H FC +C
Sbjct: 346 KALSKHEVHYGAYATMEQVNAAGNL---------CAICQEKMHSPILLCCKHMFCEECVS 498
Query: 152 KWIGQGKRTCANCRRDI 168
+W + +RTC CR +
Sbjct: 499 EWF-ERERTCPLCRASV 546
Score = 32.0 bits (71), Expect = 0.34
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = +1
Query: 494 CNICHKVLSSPLTTPCAHNFCKVCLEGAFSGKSFIRKRAC 533
C IC + + SP+ C H FC+ C+ + F R+R C
Sbjct: 421 CAICQEKMHSPILLCCKHMFCEECV-----SEWFERERTC 525
>TC12042 similar to GB|CAB87983.1|7576235|ATH276134 Pex10p {Arabidopsis
thaliana;} , partial (19%)
Length = 582
Score = 39.3 bits (90), Expect = 0.002
Identities = 19/71 (26%), Positives = 29/71 (40%)
Frame = +1
Query: 122 GSINCSFCMQLPDRPVTTPCGHNFCLKCFEKWIGQGKRTCANCRRDIPAKIASQPRINSQ 181
G C+ C+ P T CGH FC C +W + R +P+ + S
Sbjct: 136 GVSKCTLCLSNRPHPTATSCGHVFCWNCITEWCNEKSR--------VPSLPYPYHTLKSS 291
Query: 182 LAIAIRLAKLA 192
L ++ RL + A
Sbjct: 292 LCVSFRLLESA 324
>TC15777 similar to UP|AAR99376 (AAR99376) Ring domain containing protein,
partial (34%)
Length = 566
Score = 39.3 bits (90), Expect = 0.002
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = +1
Query: 122 GSINCSFCMQLPDRPVTTPCGHNFCLKCFEKWI 154
G +C+ C+ PV T CGH +C C KW+
Sbjct: 277 GGFDCNICLDCVQDPVVTLCGHLYCWPCIYKWL 375
Score = 33.9 bits (76), Expect = 0.089
Identities = 21/76 (27%), Positives = 30/76 (38%)
Frame = +1
Query: 492 FGCNICHKVLSSPLTTPCAHNFCKVCLEGAFSGKSFIRKRACEGGRSLRAQKNVMKCPSC 551
F CNIC + P+ T C H +C C+ + S S +K CP C
Sbjct: 283 FDCNICLDCVQDPVVTLCGHLYCWPCIYKWLNFYSI---------SSDYEEKEEPICPVC 435
Query: 552 SNDIADFLQNPQVNRE 567
++I+ P RE
Sbjct: 436 KSEISQSSLVPLYGRE 483
>TC19774 weakly similar to UP|AAR92268 (AAR92268) At1g47570, partial (19%)
Length = 642
Score = 38.5 bits (88), Expect = 0.004
Identities = 16/38 (42%), Positives = 22/38 (57%), Gaps = 1/38 (2%)
Frame = +2
Query: 126 CSFCMQLPDRPVTT-PCGHNFCLKCFEKWIGQGKRTCA 162
CS C+ + VTT PC HNFC CF +W + + C+
Sbjct: 524 CSICLNIWHDVVTTGPCLHNFCNGCFSEWSRRSQAKCS 637
Score = 30.4 bits (67), Expect = 0.98
Identities = 12/25 (48%), Positives = 15/25 (60%), Gaps = 1/25 (4%)
Frame = +2
Query: 494 CNICHKVLSSPLTT-PCAHNFCKVC 517
C+IC + +TT PC HNFC C
Sbjct: 524 CSICLNIWHDVVTTGPCLHNFCNGC 598
>BP045070
Length = 386
Score = 38.1 bits (87), Expect = 0.005
Identities = 22/64 (34%), Positives = 28/64 (43%), Gaps = 1/64 (1%)
Frame = -3
Query: 102 GGPSSGEKPPAAGGILDIFDGSINCSFCMQLPDRPVTTPCGHNFCLKCF-EKWIGQGKRT 160
GG GE+ GG+ + + NC CM PCGH FC C E W+ +G
Sbjct: 249 GGGEEGEEEQ--GGVEETGVVAYNCCVCMVRHKGAAFIPCGHTFCRMCSRELWVNRG--N 82
Query: 161 CANC 164
C C
Sbjct: 81 CPLC 70
>TC10567 similar to UP|AAR99376 (AAR99376) Ring domain containing protein,
partial (27%)
Length = 423
Score = 37.7 bits (86), Expect = 0.006
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = +2
Query: 125 NCSFCMQLPDRPVTTPCGHNFCLKCFEKWI 154
+CS C+ PV T CGH +C C KW+
Sbjct: 83 DCSICLDRVQDPVVTLCGHLYCWPCIYKWL 172
Score = 35.0 bits (79), Expect = 0.040
Identities = 21/90 (23%), Positives = 37/90 (40%)
Frame = +2
Query: 489 LKEFGCNICHKVLSSPLTTPCAHNFCKVCLEGAFSGKSFIRKRACEGGRSLRAQKNVMKC 548
L F C+IC + P+ T C H +C C+ + S S +K +C
Sbjct: 71 LSGFDCSICLDRVQDPVVTLCGHLYCWPCIYKWLNFHSV---------SSDHEEKKEPQC 223
Query: 549 PSCSNDIADFLQNPQVNREMMGVVESLQSQ 578
P C ++I++ P R+ + ++Q
Sbjct: 224 PVCKSEISESSLVPLYGRDQTALPSKSKAQ 313
>AV406621
Length = 126
Score = 37.4 bits (85), Expect = 0.008
Identities = 20/47 (42%), Positives = 26/47 (54%)
Frame = +3
Query: 299 SGGYIDDEDHGEWFLYTGSGGRDLSGNKRTNKLQSFDQKFENMNEAL 345
SGGY DD D G+ +Y+G GG+D + Q F QK E N A+
Sbjct: 3 SGGYEDDVDEGDVIIYSGHGGQD------KHSRQVFHQKLEGGNLAM 125
>TC14940
Length = 781
Score = 37.0 bits (84), Expect = 0.010
Identities = 18/64 (28%), Positives = 26/64 (40%)
Frame = +2
Query: 492 FGCNICHKVLSSPLTTPCAHNFCKVCLEGAFSGKSFIRKRACEGGRSLRAQKNVMKCPSC 551
F C IC L ++T C H FCK C++ A S ++ KCP+C
Sbjct: 143 FSCPICMGPLVEEMSTRCGHIFCKACIKAAISAQN--------------------KCPTC 262
Query: 552 SNDI 555
+
Sbjct: 263 RKKV 274
Score = 33.9 bits (76), Expect = 0.089
Identities = 15/47 (31%), Positives = 22/47 (45%)
Frame = +2
Query: 125 NCSFCMQLPDRPVTTPCGHNFCLKCFEKWIGQGKRTCANCRRDIPAK 171
+C CM ++T CGH FC C + I + C CR+ + K
Sbjct: 146 SCPICMGPLVEEMSTRCGHIFCKACIKAAI-SAQNKCPTCRKKVTVK 283
>BP045442
Length = 488
Score = 35.8 bits (81), Expect = 0.023
Identities = 18/54 (33%), Positives = 21/54 (38%), Gaps = 14/54 (25%)
Frame = -1
Query: 126 CSFCMQLPDRPVTT--------------PCGHNFCLKCFEKWIGQGKRTCANCR 165
CS C Q P+ + PCGH F C EKW+ TC CR
Sbjct: 470 CSSCKQQPEHECSVCLTKFEPESEINCLPCGHLFHKACLEKWLDYWNITCPLCR 309
>AV406818
Length = 416
Score = 35.4 bits (80), Expect = 0.030
Identities = 19/82 (23%), Positives = 36/82 (43%), Gaps = 3/82 (3%)
Frame = +1
Query: 116 ILDIFDGSINCSFCMQ---LPDRPVTTPCGHNFCLKCFEKWIGQGKRTCANCRRDIPAKI 172
+L+ S C+ C++ + ++ PC H F C + W+ + C C+RD +
Sbjct: 4 VLEDNSTSGTCAVCLEDYCVGEKLRILPCCHKFHAACVDSWLTSWRTFCPVCKRDARTGL 183
Query: 173 ASQPRINSQLAIAIRLAKLAKS 194
+ P S ++ A +A S
Sbjct: 184 SEPPPSESTPLLSSTPASVASS 249
>TC13904 weakly similar to UP|Q9ZU51 (Q9ZU51) RING-H2 finger protein RHA2b,
partial (32%)
Length = 535
Score = 34.3 bits (77), Expect = 0.068
Identities = 16/44 (36%), Positives = 20/44 (45%), Gaps = 3/44 (6%)
Frame = +1
Query: 125 NCSFCMQL---PDRPVTTPCGHNFCLKCFEKWIGQGKRTCANCR 165
NC C + D+ PC H F +CFE W+ K C CR
Sbjct: 22 NCVVCQRTFKDGDQVRRLPCRHTFHRRCFEGWLHHYKFNCPLCR 153
>TC11949
Length = 780
Score = 33.9 bits (76), Expect = 0.089
Identities = 13/29 (44%), Positives = 15/29 (50%)
Frame = +3
Query: 121 DGSINCSFCMQLPDRPVTTPCGHNFCLKC 149
D + CS CM PV+ CGH FC C
Sbjct: 114 DIDLTCSICMDTVFDPVSLTCGHIFCYSC 200
Score = 28.9 bits (63), Expect = 2.9
Identities = 18/58 (31%), Positives = 24/58 (41%)
Frame = +3
Query: 494 CNICHKVLSSPLTTPCAHNFCKVCLEGAFSGKSFIRKRACEGGRSLRAQKNVMKCPSC 551
C+IC + P++ C H FC C A S +G L+A KCP C
Sbjct: 129 CSICMDTVFDPVSLTCGHIFCYSCACSAAS------VTIVDG---LKAAXPKEKCPLC 275
>BP084627
Length = 327
Score = 33.9 bits (76), Expect = 0.089
Identities = 15/39 (38%), Positives = 15/39 (38%)
Frame = -2
Query: 126 CSFCMQLPDRPVTTPCGHNFCLKCFEKWIGQGKRTCANC 164
CS C V T C H FC C K G R C C
Sbjct: 326 CSICQDRTKEVVITKCYHLFCNTCMLKVTGSRHRKCPQC 210
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.134 0.418
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,332,964
Number of Sequences: 28460
Number of extensions: 249891
Number of successful extensions: 1559
Number of sequences better than 10.0: 98
Number of HSP's better than 10.0 without gapping: 1502
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1558
length of query: 680
length of database: 4,897,600
effective HSP length: 96
effective length of query: 584
effective length of database: 2,165,440
effective search space: 1264616960
effective search space used: 1264616960
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0191.7