BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0190.5
(331 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC17175 32 0.15
TC15474 UP|Q7Y1B9 (Q7Y1B9) Ammonium transporter, complete 29 0.96
TC13201 similar to UP|Q8LRL6 (Q8LRL6) Nam-like protein 9, partia... 28 1.6
TC7909 similar to UP|CB4C_ARATH (Q9S7W1) Chlorophyll a-b binding... 28 2.1
AV765046 28 2.1
TC20116 28 2.8
TC17917 similar to GB|AAO63324.1|28950801|BT005260 At5g06770 {Ar... 27 3.6
TC18419 27 3.6
TC7944 homologue to UP|Q9ASR1 (Q9ASR1) At1g56070/T6H22_13 (Elong... 27 3.6
TC13013 27 6.2
TC10917 similar to UP|Q7M4A3 (Q7M4A3) Spermatid-specific protein... 27 6.2
TC18071 weakly similar to UP|PE67_ARATH (Q9LVL2) Peroxidase 67 p... 27 6.2
AV413745 27 6.2
TC9814 27 6.2
BP071087 27 6.2
TC10013 similar to UP|Q8LDN5 (Q8LDN5) Topoisomerase-like protein... 26 8.1
TC19122 similar to UP|Q9LXI5 (Q9LXI5) Zinc finger-like protein, ... 26 8.1
>TC17175
Length = 604
Score = 32.0 bits (71), Expect = 0.15
Identities = 21/69 (30%), Positives = 36/69 (51%), Gaps = 1/69 (1%)
Frame = +2
Query: 261 TLPNRQKKIRRNRKNENERVRVCRMRSCSWERSKSSN-KEGPEEVTTKGENRGKARTTVC 319
+LP+R+K+ RR R+ EN+ ++ ++ SW ++ N +G + G + T
Sbjct: 137 SLPDREKEERRRRRPENQLIKSQLLQQSSWFLLRTLNFVKGYWFLLLVGRHTSY*LTLFT 316
Query: 320 PRLLSRWGR 328
R LSRW R
Sbjct: 317 RRDLSRWKR 343
>TC15474 UP|Q7Y1B9 (Q7Y1B9) Ammonium transporter, complete
Length = 1950
Score = 29.3 bits (64), Expect = 0.96
Identities = 21/80 (26%), Positives = 36/80 (44%), Gaps = 9/80 (11%)
Frame = -2
Query: 260 VTLPNRQKKIRRNRKNENERVRVCRMRSCSW-----ERSKSSNKEGPEEVTTKGENRGKA 314
V L +R + R ++N++ R+R+ + C W R+++S + + +R
Sbjct: 716 VLLCHRPCGLLRIQRNQSRRIRIVSCQWCGWGWPSRPRTRASARRPGRQSRLGKRSR*GR 537
Query: 315 RTTVCPRLLSRW----GRWR 330
R V R SRW RWR
Sbjct: 536 RRIVSSRRWSRW*FRQRRWR 477
>TC13201 similar to UP|Q8LRL6 (Q8LRL6) Nam-like protein 9, partial (22%)
Length = 465
Score = 28.5 bits (62), Expect = 1.6
Identities = 14/32 (43%), Positives = 15/32 (46%)
Frame = -1
Query: 261 TLPNRQKKIRRNRKNENERVRVCRMRSCSWER 292
T PN N E RVR+CR R C W R
Sbjct: 288 TTPNPSSSSSTNM--EGPRVRICRHRRCRWGR 199
>TC7909 similar to UP|CB4C_ARATH (Q9S7W1) Chlorophyll a-b binding protein
CP29.3, chloroplast precursor (LHCII protein 4.3)
(LHCB4.3), partial (80%)
Length = 1155
Score = 28.1 bits (61), Expect = 2.1
Identities = 18/64 (28%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
Frame = +2
Query: 263 PNRQKKIRRNRKNENERVRVCRMRSCSWERS-KSSNKEGPEEVTTKGENRGKARTTVCPR 321
P+RQ +RR + ++R +CS E++ +S + GP V G+ T + R
Sbjct: 170 PSRQHHLRRRKIPRPLQIRHQESTTCSQEKTGESDTRRGPSRVVP-----GRTATRMARR 334
Query: 322 LLSR 325
+L R
Sbjct: 335 ILHR 346
>AV765046
Length = 576
Score = 28.1 bits (61), Expect = 2.1
Identities = 18/62 (29%), Positives = 28/62 (45%)
Frame = -3
Query: 253 LALWVLAVTLPNRQKKIRRNRKNENERVRVCRMRSCSWERSKSSNKEGPEEVTTKGENRG 312
++ W L+ P K +RRN K + R+ +C + CS R S + T NR
Sbjct: 460 ISFW-LSRFYPFEHKFLRRNLKRVHIRIGICFIYKCSHVRLFFSKSHRRLQETRSQRNRS 284
Query: 313 KA 314
K+
Sbjct: 283 KS 278
>TC20116
Length = 428
Score = 27.7 bits (60), Expect = 2.8
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = +1
Query: 42 LPSGFGLRCGVQDLLGVSPRP 62
+P GFGLRC V + ++PRP
Sbjct: 208 MP*GFGLRCSVDNSWCLAPRP 270
>TC17917 similar to GB|AAO63324.1|28950801|BT005260 At5g06770 {Arabidopsis
thaliana;}, partial (35%)
Length = 587
Score = 27.3 bits (59), Expect = 3.6
Identities = 13/35 (37%), Positives = 18/35 (51%)
Frame = -1
Query: 14 HGLPCLPQQVVSEPMVGVAMVTAPCVESLPSGFGL 48
HGL LP+ VV + + G+ + P PSG L
Sbjct: 209 HGLDLLPKPVVKDSISGLDFLAPPLPLKAPSGLPL 105
>TC18419
Length = 409
Score = 27.3 bits (59), Expect = 3.6
Identities = 13/22 (59%), Positives = 15/22 (68%), Gaps = 1/22 (4%)
Frame = -2
Query: 311 RGKAR-TTVCPRLLSRWGRWRG 331
RG+A+ V LL RWGRWRG
Sbjct: 342 RGRAKHLAVVIGLLFRWGRWRG 277
>TC7944 homologue to UP|Q9ASR1 (Q9ASR1) At1g56070/T6H22_13 (Elongation
factor EF-2), complete
Length = 2971
Score = 27.3 bits (59), Expect = 3.6
Identities = 13/39 (33%), Positives = 24/39 (61%), Gaps = 1/39 (2%)
Frame = -1
Query: 266 QKKIRRNRKNENERVRVCRMRSCSWE-RSKSSNKEGPEE 303
+KK+R+ ++ + +RV++C R WE R + S+ G E
Sbjct: 121 KKKLRKMKRGKGKRVQICLGREKRWENRVRKSD*RGKFE 5
>TC13013
Length = 589
Score = 26.6 bits (57), Expect = 6.2
Identities = 12/26 (46%), Positives = 18/26 (69%), Gaps = 2/26 (7%)
Frame = +3
Query: 214 VVEGLTFEGEI--VGIKCWSQVPHQR 237
+V G+ GE+ +G+KC S+VPH R
Sbjct: 225 LVGGVEEMGELLRIGVKCTSEVPHAR 302
>TC10917 similar to UP|Q7M4A3 (Q7M4A3) Spermatid-specific protein T2
precursor, partial (21%)
Length = 589
Score = 26.6 bits (57), Expect = 6.2
Identities = 7/21 (33%), Positives = 17/21 (80%)
Frame = -3
Query: 268 KIRRNRKNENERVRVCRMRSC 288
+I++N+K +N++ ++C +R C
Sbjct: 425 EIKKNKKTKNKKQKLCNLRCC 363
>TC18071 weakly similar to UP|PE67_ARATH (Q9LVL2) Peroxidase 67 precursor
(Atperox P67) (ATP44) , partial (24%)
Length = 564
Score = 26.6 bits (57), Expect = 6.2
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = -2
Query: 59 SPRPCPMGPRRDSPQQVVSE 78
SP+ PMGP R SPQ VS+
Sbjct: 314 SPQRKPMGPPRRSPQSEVSD 255
>AV413745
Length = 405
Score = 26.6 bits (57), Expect = 6.2
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = -2
Query: 270 RRNRKNENERVRVCRMRSCSWERSKSSNK 298
RR+R+N R R C SCS + SS++
Sbjct: 233 RRSRRNSVSRFRACSRLSCSSLAASSSSR 147
>TC9814
Length = 1026
Score = 26.6 bits (57), Expect = 6.2
Identities = 14/45 (31%), Positives = 22/45 (48%)
Frame = +1
Query: 272 NRKNENERVRVCRMRSCSWERSKSSNKEGPEEVTTKGENRGKART 316
NR+N + +R + S+ + KSS EG E + +G RT
Sbjct: 382 NRRNADNLLRKAALNVKSYGQPKSSVHEGYESSANSDDGKGYQRT 516
>BP071087
Length = 380
Score = 26.6 bits (57), Expect = 6.2
Identities = 9/31 (29%), Positives = 19/31 (61%)
Frame = -2
Query: 188 SVPFLTVERTAVQGWIASADGRTMGDVVEGL 218
S+ F+T+ + A+ +A +G G ++EG+
Sbjct: 304 SIEFITIRKQAITSGVAVKEGAICGQMLEGI 212
>TC10013 similar to UP|Q8LDN5 (Q8LDN5) Topoisomerase-like protein, partial
(12%)
Length = 599
Score = 26.2 bits (56), Expect = 8.1
Identities = 18/67 (26%), Positives = 32/67 (46%), Gaps = 3/67 (4%)
Frame = -3
Query: 236 QRSQDVVSKISL---VFPLCLALWVLAVTLPNRQKKIRRNRKNENERVRVCRMRSCSWER 292
+R + +VS +S FPL ++ LA N Q+KI ++ + + + CSW
Sbjct: 369 RRRRQIVSILSSNLEYFPLPISQKPLARRHLNSQRKITTTSQSSEDEMEIRTGIFCSWPG 190
Query: 293 SKSSNKE 299
S++ E
Sbjct: 189 SRAKTGE 169
>TC19122 similar to UP|Q9LXI5 (Q9LXI5) Zinc finger-like protein, partial
(58%)
Length = 737
Score = 26.2 bits (56), Expect = 8.1
Identities = 18/57 (31%), Positives = 24/57 (41%), Gaps = 12/57 (21%)
Frame = +3
Query: 8 VSCSSAHGLPCLPQQVVSEPMVGVAMVTAPCVESLPS------------GFGLRCGV 52
+S S A PC +EP+V V +AP + PS GF RCG+
Sbjct: 372 LSLSPAIPQPCSSSATTTEPVVVVVAPSAPSEKEGPSRCATCRRRVGLTGFKCRCGL 542
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.136 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,406,117
Number of Sequences: 28460
Number of extensions: 86137
Number of successful extensions: 520
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 511
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 520
length of query: 331
length of database: 4,897,600
effective HSP length: 91
effective length of query: 240
effective length of database: 2,307,740
effective search space: 553857600
effective search space used: 553857600
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0190.5