BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0182.6
(451 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9686 similar to GB|CAA20565.1|3549654|ATT16L1 metal-transporti... 189 6e-49
TC11343 similar to PIR|B96660|B96660 protein F2K11.18 [imported]... 146 6e-36
TC10275 similar to UP|Q7Y051 (Q7Y051) Paa2 P-type ATPase, partia... 129 1e-30
TC16434 similar to UP|Q941L1 (Q941L1) Copper-transporting P-type... 80 5e-16
TC11467 similar to PIR|B96660|B96660 protein F2K11.18 [imported]... 80 9e-16
BP075244 62 1e-10
AV409298 60 6e-10
TC19703 weakly similar to PIR|B96660|B96660 protein F2K11.18 [im... 57 5e-09
TC10412 homologue to UP|Q9FVE8 (Q9FVE8) Plasma membrane Ca2+-ATP... 46 1e-05
TC17656 similar to GB|BAA97361.1|8843813|AB023042 Ca2+-transport... 46 1e-05
BP066255 36 0.015
AU088926 32 0.28
AV409404 30 0.62
AV780289 29 1.4
TC14073 homologue to UP|AAQ08403 (AAQ08403) Methionine synthase,... 28 3.1
CN825552 28 3.1
AV418179 27 5.3
BP073178 27 9.0
>TC9686 similar to GB|CAA20565.1|3549654|ATT16L1 metal-transporting P-type
ATPase (fragment) {Arabidopsis thaliana;} , partial (7%)
Length = 714
Score = 189 bits (481), Expect = 6e-49
Identities = 94/94 (100%), Positives = 94/94 (100%)
Frame = +3
Query: 358 TMTTVKQNLWWAFIYNIVGIPIAAGVLFPVNGTMLTPSIAGALMGLSSIGVMTNSLLLRF 417
TMTTVKQNLWWAFIYNIVGIPIAAGVLFPVNGTMLTPSIAGALMGLSSIGVMTNSLLLRF
Sbjct: 3 TMTTVKQNLWWAFIYNIVGIPIAAGVLFPVNGTMLTPSIAGALMGLSSIGVMTNSLLLRF 182
Query: 418 KFSSKQKQILDMLPKTKIHVDSDRTPQNQKMKYK 451
KFSSKQKQILDMLPKTKIHVDSDRTPQNQKMKYK
Sbjct: 183 KFSSKQKQILDMLPKTKIHVDSDRTPQNQKMKYK 284
>TC11343 similar to PIR|B96660|B96660 protein F2K11.18 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(21%)
Length = 909
Score = 146 bits (369), Expect = 6e-36
Identities = 81/210 (38%), Positives = 127/210 (59%), Gaps = 1/210 (0%)
Frame = +2
Query: 209 LQEVECKNESFVYVGVNDTLAGLIYFEDEVREDARHVVDTLSKQDISVYMLSGDKRNAAE 268
L+++E ++ + V ++ + G++ D ++ +AR VV L+ +I M++GD A
Sbjct: 104 LEKIESLAQTGILVSLDGDVIGVLAVSDPLKPNAREVVSILNSMNIRSIMVTGDNWGTAN 283
Query: 269 HVASLVGIPKDKVLSGVKPDQKKKFINELQKDNI-VAMVGDGINDAAALAASHIGIALGG 327
+A GI + V++ +P K + ELQ VAMVGDGIND+ AL A+ +GIA+G
Sbjct: 284 SIARQAGI--ETVIAEAQPQTKATKVKELQTSGYTVAMVGDGINDSPALVAADVGIAIGA 457
Query: 328 GVGAASEVSSIILMRDHLSQLLDALELSRLTMTTVKQNLWWAFIYNIVGIPIAAGVLFPV 387
G A E S I+LMR +L ++ AL+L++ T + ++ N WA YN++ IPIAAG+L+P
Sbjct: 458 GTDIAIEASDIVLMRSNLEDIIIALDLAKKTFSRIRLNYLWALGYNLLAIPIAAGILYPF 637
Query: 388 NGTMLTPSIAGALMGLSSIGVMTNSLLLRF 417
L P IAG M SS+ V+ +SLLL+F
Sbjct: 638 IRFRLHPWIAGVAMAASSVRVVCSSLLLKF 727
>TC10275 similar to UP|Q7Y051 (Q7Y051) Paa2 P-type ATPase, partial (16%)
Length = 724
Score = 129 bits (323), Expect = 1e-30
Identities = 73/150 (48%), Positives = 103/150 (68%), Gaps = 3/150 (2%)
Frame = +1
Query: 279 DKVLSGVKPDQKKKFINELQK-DNIVAMVGDGINDAAALAASHIGIALGGGV--GAASEV 335
D V + + P QK +FI+ L+ + VAMVGDGINDA ALA + +GIAL AAS+
Sbjct: 1 DFVKASLAPQQKSEFISSLKAAGHHVAMVGDGINDAPALAVADVGIALQNEAQENAASDA 180
Query: 336 SSIILMRDHLSQLLDALELSRLTMTTVKQNLWWAFIYNIVGIPIAAGVLFPVNGTMLTPS 395
+SIIL+ + +SQ++DA++L++ TM V QNL WA YN++ IPIAAGVL P +TPS
Sbjct: 181 ASIILLGNKISQVVDAIDLAQSTMAKVYQNLSWAVAYNVIAIPIAAGVLLPHFDFAMTPS 360
Query: 396 IAGALMGLSSIGVMTNSLLLRFKFSSKQKQ 425
++G +M LSSI V++NSLLL+ S ++
Sbjct: 361 LSGGMMALSSIFVVSNSLLLQLHGSQTSRK 450
>TC16434 similar to UP|Q941L1 (Q941L1) Copper-transporting P-type ATPase,
partial (10%)
Length = 579
Score = 80.5 bits (197), Expect = 5e-16
Identities = 40/99 (40%), Positives = 66/99 (66%)
Frame = +2
Query: 332 ASEVSSIILMRDHLSQLLDALELSRLTMTTVKQNLWWAFIYNIVGIPIAAGVLFPVNGTM 391
A E ++ +LMR++L ++ A++LSR T + ++ N +A YN+V IP+AAGVL+P G
Sbjct: 8 AIEAANFVLMRNNLEDVITAIDLSRKTFSRIRLNYVFAMAYNVVAIPVAAGVLYPSLGIK 187
Query: 392 LTPSIAGALMGLSSIGVMTNSLLLRFKFSSKQKQILDML 430
L P ++GA M LSS+ V+ +SLLL+ + +L++L
Sbjct: 188 LPPWVSGACMALSSVSVVCSSLLLKRYRRPRLTAVLEIL 304
>TC11467 similar to PIR|B96660|B96660 protein F2K11.18 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(7%)
Length = 396
Score = 79.7 bits (195), Expect = 9e-16
Identities = 38/78 (48%), Positives = 57/78 (72%)
Frame = +2
Query: 340 LMRDHLSQLLDALELSRLTMTTVKQNLWWAFIYNIVGIPIAAGVLFPVNGTMLTPSIAGA 399
L++ L+ ++ A++LSR TM+ ++ N WA YNI+G+PIAAGVL+P G L P +AGA
Sbjct: 2 LVKSSLADVITAIDLSRKTMSRIRLNYIWALGYNILGMPIAAGVLYPFTGIRLPPWLAGA 181
Query: 400 LMGLSSIGVMTNSLLLRF 417
M SS+ V+++SLLL+F
Sbjct: 182 CMAASSLSVVSSSLLLQF 235
>BP075244
Length = 526
Score = 62.4 bits (150), Expect = 1e-10
Identities = 30/51 (58%), Positives = 37/51 (71%)
Frame = -2
Query: 367 WWAFIYNIVGIPIAAGVLFPVNGTMLTPSIAGALMGLSSIGVMTNSLLLRF 417
+WAF YN++GIPI AGVLFP G L P IAGA M SS+ V+ SLLL++
Sbjct: 507 FWAFAYNLLGIPIPAGVLFPSTGFRLPPWIAGAAMAASSVSVVCCSLLLKY 355
>AV409298
Length = 332
Score = 60.5 bits (145), Expect = 6e-10
Identities = 27/27 (100%), Positives = 27/27 (100%)
Frame = +3
Query: 1 VAGYFTYGVMAVSVTTFTFWSLFGTHI 27
VAGYFTYGVMAVSVTTFTFWSLFGTHI
Sbjct: 252 VAGYFTYGVMAVSVTTFTFWSLFGTHI 332
>TC19703 weakly similar to PIR|B96660|B96660 protein F2K11.18 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(5%)
Length = 554
Score = 57.4 bits (137), Expect = 5e-09
Identities = 29/81 (35%), Positives = 45/81 (54%)
Frame = +3
Query: 336 SSIILMRDHLSQLLDALELSRLTMTTVKQNLWWAFIYNIVGIPIAAGVLFPVNGTMLTPS 395
+ ++L+R L L L++ T ++ N WA YN+ IP+AAG L+P+ L P
Sbjct: 3 AGVVLVRSCLGDALVLTSLAKAAFTRIRLNYGWALGYNVAAIPVAAGALYPLLERRLPPY 182
Query: 396 IAGALMGLSSIGVMTNSLLLR 416
+A MG SS+ V+ +L LR
Sbjct: 183 MAAVAMGASSLCVVGCALTLR 245
>TC10412 homologue to UP|Q9FVE8 (Q9FVE8) Plasma membrane Ca2+-ATPase,
partial (33%)
Length = 1061
Score = 45.8 bits (107), Expect = 1e-05
Identities = 23/77 (29%), Positives = 43/77 (54%), Gaps = 1/77 (1%)
Frame = +3
Query: 302 IVAMVGDGINDAAALAASHIGIALG-GGVGAASEVSSIILMRDHLSQLLDALELSRLTMT 360
+VA+ GDG NDA AL + IG+A+G G A E + +I++ D+ S ++ + R
Sbjct: 654 VVAVTGDGTNDAPALHEADIGLAMGIAGTEVAKESADVIILDDNFSTIVTVAKWGRSVYI 833
Query: 361 TVKQNLWWAFIYNIVGI 377
+++ + + N+V +
Sbjct: 834 NIQKFVQFQLTVNVVAL 884
>TC17656 similar to GB|BAA97361.1|8843813|AB023042 Ca2+-transporting
ATPase-like protein {Arabidopsis thaliana;} , partial
(25%)
Length = 821
Score = 45.8 bits (107), Expect = 1e-05
Identities = 26/71 (36%), Positives = 42/71 (58%), Gaps = 2/71 (2%)
Frame = +3
Query: 281 VLSGVKPDQKKKFINELQ-KDNIVAMVGDGINDAAALAASHIGIALG-GGVGAASEVSSI 338
V+ P+ K + L+ K ++VA+ GDG NDA AL + IG+A+G G A E S I
Sbjct: 60 VMGRSSPNDKLLLVQALRRKGHVVAVTGDGTNDAPALHEADIGLAMGIAGTEVAKESSDI 239
Query: 339 ILMRDHLSQLL 349
I++ D+ + ++
Sbjct: 240 IILDDNFASVV 272
>BP066255
Length = 540
Score = 35.8 bits (81), Expect = 0.015
Identities = 34/122 (27%), Positives = 48/122 (38%)
Frame = +2
Query: 8 GVMAVSVTTFTFWSLFGTHILPATAYQGSAVSLALQFACSVLVVACPCALGLATPTAVLV 67
G+++ + +FWS G L Y AV+ ++VVA P L LA ++
Sbjct: 209 GLVSHKLQQDSFWSWTGDDALEMLEYFAIAVT--------IVVVAVPEGLPLAVTLSLAF 364
Query: 68 GTSLGAKRGLLLRGGNILEKFAMVNAVVFDKTGTLTVGRPVVTKVVASTCIENANSSQTI 127
L+R E + DKTGTLT V K TCI +SQ +
Sbjct: 365 AMKKMMNDKALVRHLAACETMGSATTICSDKTGTLTTNHMTVVK----TCI--CMNSQEV 526
Query: 128 EN 129
N
Sbjct: 527 SN 532
>AU088926
Length = 487
Score = 31.6 bits (70), Expect = 0.28
Identities = 27/116 (23%), Positives = 47/116 (40%), Gaps = 29/116 (25%)
Frame = +2
Query: 230 GLIYFEDEVREDARHVVDTLSKQDISVYMLSGDKRNAAEHV------------------- 270
GL+ D R D+ + ++V M++GD+ +
Sbjct: 23 GLLPLFDPPRHDSAXTIRRALDLGVNVKMITGDQLAIGKETGRRLGMGTNMXPSSSLLGQ 202
Query: 271 ---ASLVGIPKDKVL------SGVKPDQKKKFINEL-QKDNIVAMVGDGINDAAAL 316
A++ IP D+++ +GV P+ K + + L +I M G G+NDA AL
Sbjct: 203 SKDAAIASIPIDELIEKADGFAGVFPEHKXEIVXRLXDTKHICGMTGXGVNDAPAL 370
>AV409404
Length = 421
Score = 30.4 bits (67), Expect = 0.62
Identities = 16/57 (28%), Positives = 26/57 (45%)
Frame = +1
Query: 78 LLRGGNILEKFAMVNAVVFDKTGTLTVGRPVVTKVVASTCIENANSSQTIENALSDV 134
L+R + E N + DKTGTLT VV K+ ++++E S++
Sbjct: 49 LVRHLSACETMGSANCICTDKTGTLTTNHMVVDKIWICEKTTEIKGNESVEKLKSEI 219
>AV780289
Length = 445
Score = 29.3 bits (64), Expect = 1.4
Identities = 16/50 (32%), Positives = 25/50 (50%)
Frame = -1
Query: 303 VAMVGDGINDAAALAASHIGIALGGGVGAASEVSSIILMRDHLSQLLDAL 352
+ +GDG ND L + +GIAL G V+S+I + + DA+
Sbjct: 403 IMAIGDGENDIEMLELASLGIALTNGSERTKLVASVIGLSHDEDGVADAI 254
>TC14073 homologue to UP|AAQ08403 (AAQ08403) Methionine synthase, complete
Length = 2757
Score = 28.1 bits (61), Expect = 3.1
Identities = 10/31 (32%), Positives = 17/31 (54%)
Frame = -2
Query: 297 LQKDNIVAMVGDGINDAAALAASHIGIALGG 327
L DNI + DG++D + +H+ + L G
Sbjct: 2189 LNGDNISIHINDGVDDVVEVGVAHVSVDLSG 2097
>CN825552
Length = 731
Score = 28.1 bits (61), Expect = 3.1
Identities = 19/75 (25%), Positives = 32/75 (42%), Gaps = 2/75 (2%)
Frame = +3
Query: 211 EVECKNESFVYVGVNDTLAGLIYFEDEVREDARHVVDTLSK--QDISVYMLSGDKRNAAE 268
+V C+ S+ Y + + I+ V L K + +Y+ G+ R E
Sbjct: 315 QVGCRFGSYAYWWIKQAIRKAIFHHSRTIRLPEKVYTQLGKILEAKKLYIQEGNLRPTKE 494
Query: 269 HVASLVGIPKDKVLS 283
+A VGI +DK+ S
Sbjct: 495 DLARRVGISEDKISS 539
>AV418179
Length = 360
Score = 27.3 bits (59), Expect = 5.3
Identities = 11/31 (35%), Positives = 18/31 (57%)
Frame = -3
Query: 341 MRDHLSQLLDALELSRLTMTTVKQNLWWAFI 371
+R S L + L ++ +T TT N+WW F+
Sbjct: 310 LRGIFSTLQN*LGVNSITTTTTTTNIWWRFL 218
>BP073178
Length = 389
Score = 26.6 bits (57), Expect = 9.0
Identities = 14/52 (26%), Positives = 27/52 (51%)
Frame = -2
Query: 284 GVKPDQKKKFINELQKDNIVAMVGDGINDAAALAASHIGIALGGGVGAASEV 335
G+ ++KK+ + + + G G ND+ ++ ++ G A GGG G + V
Sbjct: 388 GIPYEKKKRKRSAVTAAGSSSGGGGGYNDSCPVSVANSGGAAGGGTGDTTPV 233
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.134 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,326,791
Number of Sequences: 28460
Number of extensions: 75496
Number of successful extensions: 378
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 377
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 377
length of query: 451
length of database: 4,897,600
effective HSP length: 93
effective length of query: 358
effective length of database: 2,250,820
effective search space: 805793560
effective search space used: 805793560
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0182.6