BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0181.4
(987 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP042991 144 9e-35
TC8214 93 2e-19
BP057320 40 0.002
BI420337 38 0.009
TC11549 similar to UP|AAS51549 (AAS51549) ADL371Cp, partial (5%) 38 0.009
BP049447 37 0.012
TC11304 similar to PIR|T04669|T04669 serine O-acetyltransferase ... 36 0.034
TC8260 similar to UP|Q94ES8 (Q94ES8) Nodule extensin (Fragment),... 34 0.13
TC10928 weakly similar to PIR|G96668|G96668 protein F1N19.7 [imp... 33 0.17
TC12751 similar to UP|LEU3_BRANA (P29102) 3-isopropylmalate dehy... 33 0.22
AU251550 33 0.22
AV427911 33 0.29
AV777431 33 0.29
TC12132 similar to UP|AAR14275 (AAR14275) Predicted protein, par... 33 0.29
TC18264 similar to UP|Q9SP93 (Q9SP93) Thiol protease, partial (4%) 32 0.38
TC17819 weakly similar to PIR|T05606|T05606 protein kinase homol... 32 0.38
TC10164 weakly similar to UP|O24099 (O24099) MtN12 protein (Frag... 32 0.49
BP041840 32 0.64
BP030058 31 0.84
BI419489 31 1.1
>BP042991
Length = 534
Score = 144 bits (362), Expect = 9e-35
Identities = 83/149 (55%), Positives = 91/149 (60%), Gaps = 37/149 (24%)
Frame = +1
Query: 1 MISMPPVRDSPVMPTVLDAIDAKLASMATKLQVDFDTRISAISGLIDQVSFLNGHLRSIP 60
MISMPPVRDSPV+PTVLDAIDAKLASMATKLQ+DF+++IS ISGLIDQVS LN HLR IP
Sbjct: 88 MISMPPVRDSPVLPTVLDAIDAKLASMATKLQIDFESKISTISGLIDQVSSLNCHLRDIP 267
Query: 61 PQPPPI--------------YHHNPAPY---------HNYPSQTQHS------------- 84
PQPPP Y+ PY H YP HS
Sbjct: 268 PQPPPFTYSFTSSYPQPAPDYYSPSQPYIPQPNVSSQHYYPYIYPHSSPPHDTQLSFSSP 447
Query: 85 -ALSSPPLKLIKFSGSDPTFWLLNTEVFF 112
A S P LK+ F+GSDPT WLLNTE FF
Sbjct: 448 RAKSQPKLKITVFNGSDPTMWLLNTETFF 534
>TC8214
Length = 473
Score = 92.8 bits (229), Expect = 2e-19
Identities = 50/78 (64%), Positives = 54/78 (69%)
Frame = +3
Query: 910 LAIGRGYGYCFFEFQLKIHELELDKSCFVRFRPEFNSRTLYSFLGFCSHSTTLLEWILII 969
L IGR G CF E+ELD + + EF S+TLYSF G C HSTTLLEWILII
Sbjct: 9 LXIGRXIGTCF-------SEMELDHTYLLCSMSEFTSQTLYSFHGLC-HSTTLLEWILII 164
Query: 970 AWIVYLSSLRTRMFSRGW 987
AWIVY SSLRTRMFSRGW
Sbjct: 165 AWIVYFSSLRTRMFSRGW 218
>BP057320
Length = 433
Score = 39.7 bits (91), Expect = 0.002
Identities = 21/41 (51%), Positives = 24/41 (58%), Gaps = 14/41 (34%)
Frame = -3
Query: 961 TLLEWILIIAWIVYL--------------SSLRTRMFSRGW 987
+LL WILIIAW+ + SSLRTRMFSRGW
Sbjct: 356 SLLTWILIIAWLAHSCLILGQIIWVHLSNSSLRTRMFSRGW 234
Score = 28.9 bits (63), Expect = 4.2
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = -3
Query: 745 TLLTWTLLLAWPHLGCFIFGQ 765
+LLTW L++AW C I GQ
Sbjct: 356 SLLTWILIIAWLAHSCLILGQ 294
>BI420337
Length = 397
Score = 37.7 bits (86), Expect = 0.009
Identities = 17/50 (34%), Positives = 26/50 (52%), Gaps = 1/50 (2%)
Frame = +1
Query: 53 NGHLRSIPPQPPPIYHHNP-APYHNYPSQTQHSALSSPPLKLIKFSGSDP 101
N ++ + PP P P Y+H+P P H+ P +S+ PP K K+ P
Sbjct: 85 NNYIYTSPPPPKPYYYHSPPPPMHSPPPPYHYSSPPPPPKKPYKYPSPPP 234
>TC11549 similar to UP|AAS51549 (AAS51549) ADL371Cp, partial (5%)
Length = 989
Score = 37.7 bits (86), Expect = 0.009
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = -2
Query: 553 LHDSLPVIFDPGIINFEHFCLIIFHMSHAYATCMC 587
+H + FD GIIN E+ CL++F + HA++ C
Sbjct: 886 VHVFSSMTFDSGIINIENLCLVVFQLHHAFSDIAC 782
>BP049447
Length = 478
Score = 37.4 bits (85), Expect = 0.012
Identities = 24/62 (38%), Positives = 31/62 (49%), Gaps = 4/62 (6%)
Frame = +3
Query: 48 QVSFLNGHLRSIPPQPPPIYHHNPAP---YHNYPSQ-TQHSALSSPPLKLIKFSGSDPTF 103
Q F N R PP PPP +HH+P P +H PS TQ S + P +L + P F
Sbjct: 195 QQPFSNLPSRPPPPPPPPQHHHHPDPKFTHHEQPSSTTQPSVRPNLP*RLPGANALHPPF 374
Query: 104 WL 105
+L
Sbjct: 375 FL 380
>TC11304 similar to PIR|T04669|T04669 serine O-acetyltransferase F8D20.150
- Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (24%)
Length = 775
Score = 35.8 bits (81), Expect = 0.034
Identities = 15/31 (48%), Positives = 16/31 (51%)
Frame = +3
Query: 60 PPQPPPIYHHNPAPYHNYPSQTQHSALSSPP 90
PP PPP +HHN PY N T H PP
Sbjct: 267 PPPPPPPHHHNSLPYFN---PTSHGLCEPPP 350
>TC8260 similar to UP|Q94ES8 (Q94ES8) Nodule extensin (Fragment), partial
(79%)
Length = 1017
Score = 33.9 bits (76), Expect = 0.13
Identities = 14/30 (46%), Positives = 15/30 (49%)
Frame = +3
Query: 61 PQPPPIYHHNPAPYHNYPSQTQHSALSSPP 90
P P P+YH P P H YP H SPP
Sbjct: 429 PHPHPVYHSPPPPVHTYPH--PHPVYHSPP 512
Score = 31.6 bits (70), Expect = 0.64
Identities = 23/68 (33%), Positives = 25/68 (35%), Gaps = 8/68 (11%)
Frame = +3
Query: 60 PPQPPPI----YHHNPAPYHNYPSQTQHSALSS----PPLKLIKFSGSDPTFWLLNTEVF 111
PP PPP Y P P H YP H S PP K K+S P V
Sbjct: 264 PPPPPPHKPYKYPSPPPPPHKYPHPHPHPVYHSPPPPPPKKHYKYSSPPP-------PVH 422
Query: 112 FLQHPWPL 119
HP P+
Sbjct: 423 TYPHPHPV 446
Score = 30.0 bits (66), Expect = 1.9
Identities = 17/49 (34%), Positives = 23/49 (46%), Gaps = 3/49 (6%)
Frame = +3
Query: 56 LRSIPP---QPPPIYHHNPAPYHNYPSQTQHSALSSPPLKLIKFSGSDP 101
+ S PP +P P+YH +P P + P HS PP K K+ P
Sbjct: 171 VHSPPPPYEKPHPVYH-SPPPPYEKPHPVYHSPPPPPPHKPYKYPSPPP 314
Score = 29.6 bits (65), Expect = 2.4
Identities = 17/59 (28%), Positives = 23/59 (38%)
Frame = +3
Query: 61 PQPPPIYHHNPAPYHNYPSQTQHSALSSPPLKLIKFSGSDPTFWLLNTEVFFLQHPWPL 119
P P P+YH P P +H SSPP + + P + V HP P+
Sbjct: 336 PHPHPVYHSPPPP-----PPKKHYKYSSPPPPVHTYPHPHPVYHSPPPPVHTYPHPHPV 497
Score = 29.6 bits (65), Expect = 2.4
Identities = 26/88 (29%), Positives = 36/88 (40%), Gaps = 4/88 (4%)
Frame = +3
Query: 7 VRDSPVMPTVLDAIDAKLASMATKLQVDFDTRISAISGLIDQVSFLNGHLRSIPPQPPP- 65
+R M +++ +I LA T + F + ISA N + S PP PP
Sbjct: 3 IRHEQEMGSLMASISITLA--LTIMLFSFPSEISA-----------NHYAYSSPPPPPKP 143
Query: 66 -IYHHNPAPYHNYPS--QTQHSALSSPP 90
YH P P H+ P + H SPP
Sbjct: 144YSYHSPPPPVHSPPPPYEKPHPVYHSPP 227
Score = 28.9 bits (63), Expect = 4.2
Identities = 15/37 (40%), Positives = 17/37 (45%), Gaps = 6/37 (16%)
Frame = +3
Query: 60 PPQPP------PIYHHNPAPYHNYPSQTQHSALSSPP 90
PP PP P+YH P P H+ P H SPP
Sbjct: 552 PPPPPVHTYPHPVYHSPPPPVHSPP--PPHYYYKSPP 656
>TC10928 weakly similar to PIR|G96668|G96668 protein F1N19.7 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(29%)
Length = 590
Score = 33.5 bits (75), Expect = 0.17
Identities = 14/36 (38%), Positives = 19/36 (51%)
Frame = -3
Query: 67 YHHNPAPYHNYPSQTQHSALSSPPLKLIKFSGSDPT 102
+HHNP +P T H+ +S P + S SDPT
Sbjct: 459 FHHNPNKAQTWPHHTHHNHISLPLSPHVSHSRSDPT 352
>TC12751 similar to UP|LEU3_BRANA (P29102) 3-isopropylmalate dehydrogenase,
chloroplast precursor (Beta-IPM dehydrogenase) (IMDH)
(3-IPM-DH) , partial (25%)
Length = 450
Score = 33.1 bits (74), Expect = 0.22
Identities = 23/78 (29%), Positives = 33/78 (41%), Gaps = 12/78 (15%)
Frame = +3
Query: 63 PPPIYHHNPAPYHNYP--------SQTQHSALSSPPLKLIKFSGSDPTFW----LLNTEV 110
PPP NP P +P S + + SSP L + S S+ FW L+ E
Sbjct: 162 PPPPLRKNPTPSLFFPATESALKSSPSLKTFFSSPALSMGSISSSERCFWVELPLMLRES 341
Query: 111 FFLQHPWPLELRFQFIAL 128
+L P+PL+ +L
Sbjct: 342 PYLTRPFPLQSSLMLFSL 395
>AU251550
Length = 329
Score = 33.1 bits (74), Expect = 0.22
Identities = 13/30 (43%), Positives = 15/30 (49%)
Frame = +2
Query: 61 PQPPPIYHHNPAPYHNYPSQTQHSALSSPP 90
P PPP HH+P P H PS + PP
Sbjct: 176 PPPPPFGHHHPPPPHGPPSPPPPAMFYQPP 265
Score = 27.7 bits (60), Expect = 9.3
Identities = 13/31 (41%), Positives = 15/31 (47%)
Frame = +2
Query: 60 PPQPPPIYHHNPAPYHNYPSQTQHSALSSPP 90
PP PPP +HNP P P Q +PP
Sbjct: 59 PPGPPPPGYHNPPP----PGPPQPPGYYNPP 139
>AV427911
Length = 348
Score = 32.7 bits (73), Expect = 0.29
Identities = 17/42 (40%), Positives = 19/42 (44%)
Frame = +1
Query: 60 PPQPPPIYHHNPAPYHNYPSQTQHSALSSPPLKLIKFSGSDP 101
PP+ P Y P P H YP T HS PP K K+ P
Sbjct: 205 PPKKPYKYPSPPPPVHVYPKPTYHSP-PPPPKKPYKYPSPPP 327
Score = 30.4 bits (67), Expect = 1.4
Identities = 17/58 (29%), Positives = 27/58 (46%), Gaps = 9/58 (15%)
Frame = +1
Query: 53 NGHLRSIPPQP-------PPIYHHNPAP--YHNYPSQTQHSALSSPPLKLIKFSGSDP 101
+G++ S PP P PP ++H+P P Y P + + + PP K K+ P
Sbjct: 70 DGYIYSSPPPPKEYPPVSPPYHYHSPPPPVYSPPPPKYYYKSPPPPPKKPYKYPSPPP 243
>AV777431
Length = 579
Score = 32.7 bits (73), Expect = 0.29
Identities = 22/62 (35%), Positives = 27/62 (43%), Gaps = 1/62 (1%)
Frame = -3
Query: 745 TLLTWTLLLAW-PHLGCFIFGQPFGFICSKFKFVLAGPTTLSLFLARLEDKTYFQRGSIG 803
+LL+W LL+AW L CF+ GQP T+ F LEDK G
Sbjct: 361 SLLSWILLIAWIAQLVCFLLGQPI--------------WTILFFQPILEDKDALPADGNG 224
Query: 804 RR 805
RR
Sbjct: 223 RR 218
Score = 27.7 bits (60), Expect = 9.3
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = -3
Query: 953 LGFCSHSTTLLEWILIIAWIVYL 975
LG ++LL WIL+IAWI L
Sbjct: 385 LGSSQVHSSLLSWILLIAWIAQL 317
>TC12132 similar to UP|AAR14275 (AAR14275) Predicted protein, partial (14%)
Length = 698
Score = 32.7 bits (73), Expect = 0.29
Identities = 22/70 (31%), Positives = 30/70 (42%)
Frame = +2
Query: 61 PQPPPIYHHNPAPYHNYPSQTQHSALSSPPLKLIKFSGSDPTFWLLNTEVFFLQHPWPLE 120
P PPP NP P NYP Q + S PL S P W T + L+ P +
Sbjct: 161 PPPPP*QPTNPNPTRNYPIHLQPTTDSDSPL------ASLPQSW---TTLTLLRFPTDIR 313
Query: 121 LRFQFIALYL 130
+R + + +L
Sbjct: 314 VRVRVMTRFL 343
>TC18264 similar to UP|Q9SP93 (Q9SP93) Thiol protease, partial (4%)
Length = 570
Score = 32.3 bits (72), Expect = 0.38
Identities = 16/33 (48%), Positives = 19/33 (57%)
Frame = -1
Query: 60 PPQPPPIYHHNPAPYHNYPSQTQHSALSSPPLK 92
PP PPP P+P H+ PSQ SSPP+K
Sbjct: 498 PPPPPP-----PSPPHSPPSQIVSGPGSSPPMK 415
>TC17819 weakly similar to PIR|T05606|T05606 protein kinase homolog
F9D16.210 - Arabidopsis thaliana {Arabidopsis
thaliana;}, partial (9%)
Length = 538
Score = 32.3 bits (72), Expect = 0.38
Identities = 31/110 (28%), Positives = 47/110 (42%), Gaps = 10/110 (9%)
Frame = +2
Query: 194 ELETTEDPQQKPQVLSDVKPHTPTFM--ALSSPVSTQKVSAGIIHIEHFCPTVFETPP-- 249
E T+ K +LS KPHTPT L +P +T VS +H P+V +PP
Sbjct: 122 ESTRTQLRTSKLSLLSSPKPHTPTASTGTLPTPPATGSVSNATLHAPSSTPSV--SPPSA 295
Query: 250 ---TKKPLDISTHCSQVISTPPQIRS---DCIINSFTPDAVFSKKYYVST 293
T P + + S V S + S +I+ +P + S +S+
Sbjct: 296 SSVTSHPTPSAGYPSSVSSASAPMASPARSLLISPISPSSAASTSRRISS 445
>TC10164 weakly similar to UP|O24099 (O24099) MtN12 protein (Fragment),
partial (66%)
Length = 566
Score = 32.0 bits (71), Expect = 0.49
Identities = 13/28 (46%), Positives = 14/28 (49%)
Frame = +3
Query: 63 PPPIYHHNPAPYHNYPSQTQHSALSSPP 90
PPPI H P P YP Q + SPP
Sbjct: 3 PPPIQHSPPPPIQTYPPQIPNPISHSPP 86
Score = 29.3 bits (64), Expect = 3.2
Identities = 14/36 (38%), Positives = 18/36 (49%), Gaps = 1/36 (2%)
Frame = +3
Query: 56 LRSIPPQ-PPPIYHHNPAPYHNYPSQTQHSALSSPP 90
+++ PPQ P PI H P P YP + SPP
Sbjct: 36 IQTYPPQIPNPISHSPPPPGQTYPPYIPNPIFLSPP 143
Score = 28.5 bits (62), Expect = 5.4
Identities = 15/44 (34%), Positives = 17/44 (38%)
Frame = +3
Query: 60 PPQPPPIYHHNPAPYHNYPSQTQHSALSSPPLKLIKFSGSDPTF 103
PP P PI H P P YP SPP I + P +
Sbjct: 138 PPPPFPISHSPPPPVQTYPPNIPIPIDHSPPPVPINHAPPPPPY 269
>BP041840
Length = 542
Score = 31.6 bits (70), Expect = 0.64
Identities = 14/39 (35%), Positives = 21/39 (52%)
Frame = -1
Query: 948 TLYSFLGFCSHSTTLLEWILIIAWIVYLSSLRTRMFSRG 986
T+ SF G TLL WI ++AW+ + S L + +G
Sbjct: 434 TVTSFPGLGLIDITLLAWIFLVAWLAHFSILEDKKVFKG 318
>BP030058
Length = 455
Score = 31.2 bits (69), Expect = 0.84
Identities = 18/47 (38%), Positives = 24/47 (50%), Gaps = 1/47 (2%)
Frame = -1
Query: 60 PPQPPPIYHHNPAPYHNYPSQTQH-SALSSPPLKLIKFSGSDPTFWL 105
PP PPP +P P YPS Q+ + LS PP ++I G W+
Sbjct: 341 PPSPPPPPPPSPPP-PKYPSCPQNCNPLSPPPPRIIYVPGQPKPSWI 204
>BI419489
Length = 479
Score = 30.8 bits (68), Expect = 1.1
Identities = 13/32 (40%), Positives = 16/32 (49%)
Frame = +3
Query: 54 GHLRSIPPQPPPIYHHNPAPYHNYPSQTQHSA 85
G LR +PP PP I HH P + P + A
Sbjct: 318 GTLRPLPPSPPGILHHQPPHCQDEPHHLRRLA 413
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.137 0.431
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,398,768
Number of Sequences: 28460
Number of extensions: 381976
Number of successful extensions: 3009
Number of sequences better than 10.0: 84
Number of HSP's better than 10.0 without gapping: 2759
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2944
length of query: 987
length of database: 4,897,600
effective HSP length: 99
effective length of query: 888
effective length of database: 2,080,060
effective search space: 1847093280
effective search space used: 1847093280
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0181.4