BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0180a.4
(342 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC11317 similar to UP|Q40363 (Q40363) NuM1 protein, partial (12%) 33 0.090
BP057817 32 0.20
BP031757 32 0.20
BP048490 31 0.34
TC18979 similar to UP|Q8LG35 (Q8LG35) EF-hand Ca2+-binding prote... 30 0.45
TC16317 similar to UP|Q8W4C5 (Q8W4C5) Nucellin-like protein, par... 30 0.77
TC17487 UP|CAE45597 (CAE45597) SAR DNA-binding protein-like prot... 30 0.77
AV418628 29 1.0
AV776363 29 1.3
BP046094 28 1.7
TC11009 similar to GB|AAP21377.1|30102918|BT006569 At1g47970 {Ar... 28 1.7
TC12711 similar to UP|Q84Y18 (Q84Y18) CAX-interacting protein 4,... 28 1.7
TC10485 28 1.7
TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (... 28 2.2
TC15506 similar to UP|Q9SEE9 (Q9SEE9) Arginine/serine-rich prote... 28 2.2
AI967639 28 2.2
TC19521 similar to UP|O22526 (O22526) Cation-chloride co-transpo... 28 2.9
TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly pr... 28 2.9
BP040001 28 2.9
BP035374 28 2.9
>TC11317 similar to UP|Q40363 (Q40363) NuM1 protein, partial (12%)
Length = 547
Score = 32.7 bits (73), Expect = 0.090
Identities = 24/88 (27%), Positives = 42/88 (47%), Gaps = 3/88 (3%)
Frame = +1
Query: 79 KREASSDSDSDSDDENDAPPPINDPYLMTLEERQEWRRKIRQVMDMKPNVQEESDPEEK- 137
K+E+SSD DS S+DE AP P +++ +K + + ++ SD EE+
Sbjct: 271 KKESSSDDDSSSEDEKPAPKVA--PLKTSVKNGTTPAKKAKPAPSSSSSDEDSSDEEEEV 444
Query: 138 --KKKMEKLMKDYPLVVDEEDPNWPEDA 163
KK + ++ +ED + ED+
Sbjct: 445 IAKKPTKVVVPKKEESSSDEDSSSDEDS 528
>BP057817
Length = 545
Score = 31.6 bits (70), Expect = 0.20
Identities = 19/41 (46%), Positives = 24/41 (58%)
Frame = -2
Query: 87 DSDSDDENDAPPPINDPYLMTLEERQEWRRKIRQVMDMKPN 127
D SD+++D PP D TLE RQ R+ I QV D +PN
Sbjct: 541 DDWSDEDDDDTPPDFDEDAGTLEIRQ--RKTINQVEDSEPN 425
>BP031757
Length = 507
Score = 31.6 bits (70), Expect = 0.20
Identities = 21/73 (28%), Positives = 35/73 (47%), Gaps = 1/73 (1%)
Frame = +2
Query: 90 SDDENDAPPPINDPYLMTLEERQEWRRKI-RQVMDMKPNVQEESDPEEKKKKMEKLMKDY 148
SDDE + PP L+ R+ R K+ + + K E + E+KKKK + K+
Sbjct: 92 SDDEEEETPP------QQLKPRKRKRMKLLEEDNEDKDGEDYEEEEEKKKKKKSRKKKEE 253
Query: 149 PLVVDEEDPNWPE 161
++E+P P+
Sbjct: 254 EEEEEQEEPESPQ 292
>BP048490
Length = 452
Score = 30.8 bits (68), Expect = 0.34
Identities = 33/123 (26%), Positives = 50/123 (39%), Gaps = 1/123 (0%)
Frame = +3
Query: 76 KSGKREASSDSDSDS-DDENDAPPPINDPYLMTLEERQEWRRKIRQVMDMKPNVQEESDP 134
K K E+S D S+S D+END+ P + + +K + +SD
Sbjct: 3 KVEKSESSDDESSESSDEENDSKPATT---------------TVAKPSAVKKSASSDSDD 137
Query: 135 EEKKKKMEKLMKDYPLVVDEEDPNWPEDADGWGFSLGQFFNKITIKDNKKAKDDDDDDDE 194
E+ ++ KD + VDE D + ED+D + K T KK KD + D
Sbjct: 138 EDSSDSDSEVKKD-KMDVDESDSS--EDSD-------EEDEKSTKTPQKKVKDVEMVDAS 287
Query: 195 GNK 197
K
Sbjct: 288 SGK 296
Score = 26.2 bits (56), Expect = 8.5
Identities = 22/85 (25%), Positives = 39/85 (45%), Gaps = 2/85 (2%)
Frame = +3
Query: 79 KREASSDSDSDSDDENDAPPPINDPYLMTLEERQEWRRKIRQVMDMKPNVQEESDPEEKK 138
K+ ASSDSD + ++D+ +K + +D + + E+SD E++K
Sbjct: 108 KKSASSDSDDEDSSDSDSEV-----------------KKDKMDVD-ESDSSEDSDEEDEK 233
Query: 139 --KKMEKLMKDYPLVVDEEDPNWPE 161
K +K +KD +V N P+
Sbjct: 234 STKTPQKKVKDVEMVDASSGKNAPK 308
>TC18979 similar to UP|Q8LG35 (Q8LG35) EF-hand Ca2+-binding protein CCD1,
partial (84%)
Length = 625
Score = 30.4 bits (67), Expect = 0.45
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = -2
Query: 183 KKAKDDDDDDDEGNKVVW 200
++ ++ DDDDDEGN VW
Sbjct: 96 RRRRESDDDDDEGNMCVW 43
>TC16317 similar to UP|Q8W4C5 (Q8W4C5) Nucellin-like protein, partial (13%)
Length = 1131
Score = 29.6 bits (65), Expect = 0.77
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = +2
Query: 190 DDDDEGNKVVWQDDDYIRPIKDIK 213
DD D + WQDD IR +KD+K
Sbjct: 482 DDSDTTFPICWQDDFPIRSVKDVK 553
>TC17487 UP|CAE45597 (CAE45597) SAR DNA-binding protein-like protein, complete
Length = 1794
Score = 29.6 bits (65), Expect = 0.77
Identities = 14/32 (43%), Positives = 22/32 (68%), Gaps = 1/32 (3%)
Frame = +1
Query: 125 KPNVQEESDPEEKKKKMEKLM-KDYPLVVDEE 155
+P+ ++ + +EKKKK EK KD PL+ DE+
Sbjct: 1507 EPSADKKKEKKEKKKKKEKNEEKDVPLLADED 1602
>AV418628
Length = 309
Score = 29.3 bits (64), Expect = 1.0
Identities = 20/46 (43%), Positives = 25/46 (53%)
Frame = +2
Query: 49 SSNLSLLVGDSKAPRRVQALKSDGAHRKSGKREASSDSDSDSDDEN 94
SS+ S DS P V++ K G +KS K SSDSDS SD +
Sbjct: 32 SSDSSSSGSDSDRPGVVRSGKKKGGSKKSKK---SSDSDSSSDSSS 160
>AV776363
Length = 427
Score = 28.9 bits (63), Expect = 1.3
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +1
Query: 75 RKSGKREASSDSDSDSDDENDAPPPIND 102
R K E DSD+DSDD++D P +D
Sbjct: 313 RGPDKGEYEDDSDTDSDDDSDEPTSNSD 396
>BP046094
Length = 547
Score = 28.5 bits (62), Expect = 1.7
Identities = 14/36 (38%), Positives = 22/36 (60%)
Frame = -3
Query: 181 DNKKAKDDDDDDDEGNKVVWQDDDYIRPIKDIKSSE 216
D + DDD+D+DE N+ V D + RP K K+++
Sbjct: 485 DEESCSDDDNDEDEENQTVCVSDRH-RPRKKQKNNK 381
Score = 26.6 bits (57), Expect = 6.5
Identities = 10/19 (52%), Positives = 13/19 (67%)
Frame = -3
Query: 76 KSGKREASSDSDSDSDDEN 94
K G E+ SD D+D D+EN
Sbjct: 494 KDGDEESCSDDDNDEDEEN 438
>TC11009 similar to GB|AAP21377.1|30102918|BT006569 At1g47970 {Arabidopsis
thaliana;}, partial (44%)
Length = 700
Score = 28.5 bits (62), Expect = 1.7
Identities = 37/145 (25%), Positives = 57/145 (38%), Gaps = 19/145 (13%)
Frame = +2
Query: 85 DSDSDSDDENDAPPPINDPYLMTLEERQ---EWRRKIRQVMDMKPNVQEESDPEEKKK-- 139
D D D DD++DAP +D EE + E R D + ++ D EE+++
Sbjct: 53 DDDGDEDDDDDAPGGGDDDDDEEDEEEEGGVEGGRGGGGDPDDDDDDDDDDDEEEEEEED 232
Query: 140 --------------KMEKLMKDYPLVVDEEDPNWPEDADGWGFSLGQFFNKITIKDNKKA 185
+ E+ D+ DE D N +D + G + + +K
Sbjct: 233 LGTEYLVRRTVAAAEDEEASSDFEPEEDEGDDNDNDDGEKAGVPSKR------KRSDKDG 394
Query: 186 KDDDDDDDEGNKVVWQDDDYIRPIK 210
DDD DD G +DD+ RP K
Sbjct: 395 SGDDDSDDGG-----EDDE--RPSK 448
>TC12711 similar to UP|Q84Y18 (Q84Y18) CAX-interacting protein 4, partial
(5%)
Length = 500
Score = 28.5 bits (62), Expect = 1.7
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = +2
Query: 70 SDGAHRKSGKREASSDSDSDSDDENDA 96
S H++ +RE SS SD+DS ND+
Sbjct: 179 SSTRHKRKSQREKSSSSDTDSSGYNDS 259
>TC10485
Length = 636
Score = 28.5 bits (62), Expect = 1.7
Identities = 10/17 (58%), Positives = 13/17 (75%)
Frame = -1
Query: 185 AKDDDDDDDEGNKVVWQ 201
+ DDDDDDDEG+ W+
Sbjct: 255 SSDDDDDDDEGSCASWR 205
>TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (SPBC3D6.14C
protein) (Fragment), partial (15%)
Length = 543
Score = 28.1 bits (61), Expect = 2.2
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = +3
Query: 180 KDNKKAKDDDDDDDEGNKVVWQDDD 204
+D++ DDDDDDD+ + +DDD
Sbjct: 279 EDDEDEDDDDDDDDDDDGEEEEDDD 353
Score = 28.1 bits (61), Expect = 2.2
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = +3
Query: 187 DDDDDDDEGNKVVWQDDD 204
DDDDDDD+G + DDD
Sbjct: 306 DDDDDDDDGEEEEDDDDD 359
Score = 26.9 bits (58), Expect = 5.0
Identities = 11/24 (45%), Positives = 14/24 (57%)
Frame = +3
Query: 181 DNKKAKDDDDDDDEGNKVVWQDDD 204
D + DDDDDDD+ + DDD
Sbjct: 285 DEDEDDDDDDDDDDDGEEEEDDDD 356
Score = 26.9 bits (58), Expect = 5.0
Identities = 19/68 (27%), Positives = 29/68 (41%)
Frame = +3
Query: 127 NVQEESDPEEKKKKMEKLMKDYPLVVDEEDPNWPEDADGWGFSLGQFFNKITIKDNKKAK 186
N +EE D EE+ + DE+D + +D D D ++ +
Sbjct: 249 NEEEEDDDEEEDDE------------DEDDDDDDDDDD----------------DGEEEE 344
Query: 187 DDDDDDDE 194
DDDDDD+E
Sbjct: 345 DDDDDDEE 368
>TC15506 similar to UP|Q9SEE9 (Q9SEE9) Arginine/serine-rich protein, partial
(24%)
Length = 677
Score = 28.1 bits (61), Expect = 2.2
Identities = 17/56 (30%), Positives = 25/56 (44%), Gaps = 5/56 (8%)
Frame = +2
Query: 58 DSKAPRRVQALKSDGAHRKSGKREASSDSDSDSDDENDAPPPINDP-----YLMTL 108
DS +PR+V + R+ + +S + S S PPP P YL+TL
Sbjct: 233 DSPSPRKVSRRSRSRSPRRPLRGGRASSNSSSSSSPPPPPPPARKP*RIPVYLLTL 400
>AI967639
Length = 335
Score = 28.1 bits (61), Expect = 2.2
Identities = 24/96 (25%), Positives = 41/96 (42%)
Frame = +1
Query: 133 DPEEKKKKMEKLMKDYPLVVDEEDPNWPEDADGWGFSLGQFFNKITIKDNKKAKDDDDDD 192
D E KK+ ++ DEED E+ G ++L ++++ DDDDDD
Sbjct: 43 DEEYLKKRKQRRDISSSSEGDEEDE---EEDQGDEYNL----------EDEEEDDDDDDD 183
Query: 193 DEGNKVVWQDDDYIRPIKDIKSSEWEETVFKDISPL 228
D+ + D D R +K + ET + + +
Sbjct: 184 DDLSISEDSDSDKPRKVKQLPGRTRRETKRRSVDEI 291
>TC19521 similar to UP|O22526 (O22526) Cation-chloride co-transporter,
partial (5%)
Length = 556
Score = 27.7 bits (60), Expect = 2.9
Identities = 19/74 (25%), Positives = 29/74 (38%), Gaps = 10/74 (13%)
Frame = -2
Query: 131 ESDPEEKKKKMEKLMKDYPLVV----------DEEDPNWPEDADGWGFSLGQFFNKITIK 180
E D EE ME + + + + + P+ P + + F + + I
Sbjct: 276 EEDEEEDPGSMEDISRTARSLAKTGRYLRPIGERKPPSAPAASTSSSAPISGFDDMVWIG 97
Query: 181 DNKKAKDDDDDDDE 194
D DDDDDDDE
Sbjct: 96 DRDDDDDDDDDDDE 55
>TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly protein
1-like protein 3, partial (81%)
Length = 1298
Score = 27.7 bits (60), Expect = 2.9
Identities = 24/84 (28%), Positives = 39/84 (45%), Gaps = 5/84 (5%)
Frame = +2
Query: 126 PNVQEESDP--EEKKKKMEKLMK-DYPLVVDEEDPNWPEDADGW--GFSLGQFFNKITIK 180
P V E+ D ++ ++++ LM+ DY + D P A W G + F I
Sbjct: 605 PQVPEDDDDIDDDAVEELQNLMEHDYDIGSTIRDKIIPH-AVSWFTGEAEQSDFEDIEED 781
Query: 181 DNKKAKDDDDDDDEGNKVVWQDDD 204
D +D+D+DDD+ + DDD
Sbjct: 782 DEDGDEDEDEDDDDDEEEEDDDDD 853
>BP040001
Length = 504
Score = 27.7 bits (60), Expect = 2.9
Identities = 16/29 (55%), Positives = 16/29 (55%)
Frame = -3
Query: 26 NMPTSYCCLHKDKPRKQFLNLSLSSNLSL 54
N PTSY H P F N SLSS LSL
Sbjct: 271 NQPTSYTE*HNRNPVIWFRNHSLSSTLSL 185
>BP035374
Length = 435
Score = 27.7 bits (60), Expect = 2.9
Identities = 17/56 (30%), Positives = 24/56 (42%), Gaps = 5/56 (8%)
Frame = -1
Query: 58 DSKAPRRVQALKSDGAHRKSGKREASSDSDSDSDDENDAPPPINDP-----YLMTL 108
DS +PR+V + R+ + +S S S PPP P YL+TL
Sbjct: 366 DSPSPRKVSRRSRSRSPRRPLRGGRASSDSSSSSSPPPPPPPARKP*RIPVYLLTL 199
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.315 0.134 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,206,109
Number of Sequences: 28460
Number of extensions: 85188
Number of successful extensions: 844
Number of sequences better than 10.0: 76
Number of HSP's better than 10.0 without gapping: 614
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 732
length of query: 342
length of database: 4,897,600
effective HSP length: 91
effective length of query: 251
effective length of database: 2,307,740
effective search space: 579242740
effective search space used: 579242740
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0180a.4