BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0177.6
(1032 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP066094 273 8e-79
BP084332 225 3e-59
AV780009 135 4e-32
BP052632 105 3e-23
BP075943 74 2e-13
BP057233 72 6e-13
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 71 8e-13
BP043850 71 1e-12
AV427921 67 1e-11
BP063694 66 2e-11
TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprot... 50 5e-11
AU088980 63 3e-10
AV766665 62 4e-10
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 60 2e-09
TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, p... 56 3e-08
TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%) 56 3e-08
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 52 6e-07
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 52 6e-07
AV424544 49 4e-06
TC19474 weakly similar to UP|Q9FJA1 (Q9FJA1) Similarity to retro... 37 1e-05
>BP066094
Length = 532
Score = 273 bits (698), Expect(3) = 8e-79
Identities = 134/149 (89%), Positives = 140/149 (93%)
Frame = +2
Query: 608 ARLEAIRLLISFSVNHNIVLHQIDVKSAFLNGYISEEVYVHQPPGFEDEKKPDHVFKLKK 667
++ EAIRLLISFSVNHNI+LHQ++VKSAFLNGYISEEVYVHQPPG EDEK DH+FKLKK
Sbjct: 86 SKTEAIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPGXEDEKNSDHIFKLKK 265
Query: 668 SLYGLKQAPRAWYERLSSFLLENEFERGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANQ 727
SLYGLKQAPRAWYERLSSFLLENE RGKVDTTLFCKTYKDDILIVQIYVDDIIFGSAN
Sbjct: 266 SLYGLKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANP 445
Query: 728 SLCKEFYEMMQAEFEMSMMGELKYFLGIQ 756
SLCKEF EMMQAEFEM MMGELKYFLGIQ
Sbjct: 446 SLCKEFSEMMQAEFEMRMMGELKYFLGIQ 532
Score = 37.4 bits (85), Expect(3) = 8e-79
Identities = 17/18 (94%), Positives = 17/18 (94%)
Frame = +3
Query: 592 YSQQEGIDYTETFAPVAR 609
YSQQ GIDYTETFAPVAR
Sbjct: 39 YSQQ*GIDYTETFAPVAR 92
Score = 23.1 bits (48), Expect(3) = 8e-79
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +1
Query: 582 RNKARLVAQG 591
RNKARLVAQG
Sbjct: 10 RNKARLVAQG 39
>BP084332
Length = 368
Score = 225 bits (574), Expect(2) = 3e-59
Identities = 111/112 (99%), Positives = 111/112 (99%)
Frame = +2
Query: 912 QSTIALSTAEAEYISAAICSTQMLWMKH*LEDYQILESNIPIYCDNTAAISLSKNPILHS 971
QSTIALSTAEAEYISAAICSTQMLWMKH LEDYQILESNIPIYCDNTAAISLSKNPILHS
Sbjct: 32 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILESNIPIYCDNTAAISLSKNPILHS 211
Query: 972 RAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFNFILKNL 1023
RAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFNFILKNL
Sbjct: 212 RAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFNFILKNL 367
Score = 21.6 bits (44), Expect(2) = 3e-59
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +1
Query: 897 CQFLGSNL 904
CQFLGSNL
Sbjct: 4 CQFLGSNL 27
>AV780009
Length = 529
Score = 135 bits (339), Expect = 4e-32
Identities = 69/168 (41%), Positives = 106/168 (63%), Gaps = 6/168 (3%)
Frame = -1
Query: 849 AVKRILRYLKGTTNLGLMYKKTSEYKLSGYCDADYAGDRTERKSTSGNCQFLGSNLVSWA 908
A R+LRY+KG GL + S KL Y D+D+AG R+S +G FLG++L+SW
Sbjct: 526 AATRVLRYVKGAPAQGLFFSADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSLISWR 347
Query: 909 SKRQSTIALSTAEAEY--ISAAICSTQMLWMKH*LEDYQILESN----IPIYCDNTAAIS 962
+K+Q+T++ S++EAEY ++A +C Q W+ + +Q L+ N +P++CDN +A+
Sbjct: 346 TKKQTTVSRSSSEAEYRALAATVCEVQ--WLSY---LFQFLKLNVPLPVPLFCDNQSALH 182
Query: 963 LSKNPILHSRAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKP 1010
++ NP H R KHIE+ H +R +Q G++ L + T HQ ADIFTKP
Sbjct: 181 IAHNPTFHERTKHIELDCHVVRAKLQAGLIHLLPISTHHQLADIFTKP 38
>BP052632
Length = 489
Score = 105 bits (263), Expect = 3e-23
Identities = 50/114 (43%), Positives = 71/114 (61%)
Frame = -3
Query: 353 KTPYELWKNIKPNISYFHPFGCVCYVLNTKDRLHKFDAKSSKCLLLGYSERSKGFRFYNT 412
KTPYELW+ +P +SYFHPFGC ++LNT D + KFD K K +LLGYS SK + +N+
Sbjct: 487 KTPYELWRGRRPTVSYFHPFGCK*FILNTNDNIGKFDNKFDKRILLGYSSNSKAYIVFNS 308
Query: 413 DAKTIEESIHVRFDDKLDSDQSKLVEKFADLSINVSDKGKAPEEVEPEEDEPEE 466
+ +EESI+V+FDD +L + F +L ++ D+ E +P E EE
Sbjct: 307 RTQVVEESINVKFDD*SIEATYRLEKDFLNLDLSDQDEEVPTNEGQPSETSHEE 146
>BP075943
Length = 547
Score = 73.6 bits (179), Expect = 2e-13
Identities = 45/159 (28%), Positives = 77/159 (48%), Gaps = 2/159 (1%)
Frame = -3
Query: 715 IYVDDIIFGSANQSLCKEFYEMMQAEFEMSMMGELKYFLGIQVDQTPEGTYIHQSKYTKE 774
+YVDD++ + + + +F + +GE KYFLG+++ ++ G ++Q KY +
Sbjct: 515 LYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEIARSTSGIVLNQRKYALQ 336
Query: 775 LLKKFNMLESTVAKTPMHPTCILEKEDKSGKVCQKL--YRGTIGSLLYLTASRPDILFSV 832
L+ + H T +G + YR +G LLYL +RPDI F+V
Sbjct: 335 LISDSGHF-GFQPRFYSHGTNSQTLGTNTGTPLTDIGSYRRIVGRLLYLNTTRPDITFAV 159
Query: 833 HLCARF*SDPRETHLTAVKRILRYLKGTTNLGLMYKKTS 871
+ ++F S P + H + L+YL G+ GL Y +S
Sbjct: 158 NQLSQFLSAPTDIHEQQLTGFLKYL*GSPGSGLFYPASS 42
>BP057233
Length = 473
Score = 71.6 bits (174), Expect = 6e-13
Identities = 32/77 (41%), Positives = 51/77 (65%)
Frame = -2
Query: 953 IYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLA 1012
++CDN +A +L+ NP+LH+R+KHIE+ H+IRD V + +++ +V T Q AD TKPL+
Sbjct: 445 LWCDNLSAKALASNPVLHARSKHIEIDVHYIRDQVLQNEVVVAYVPTTDQIADCLTKPLS 266
Query: 1013 EDRFNFILKNLNMDFCP 1029
RF+ + L + P
Sbjct: 265 HTRFSQLRDKLGVIHSP 215
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 71.2 bits (173), Expect = 8e-13
Identities = 32/91 (35%), Positives = 54/91 (59%), Gaps = 1/91 (1%)
Frame = +1
Query: 936 WMKH*LEDYQI-LESNIPIYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYVQKGVLLL 994
W+ + L+D ++ E +YCDN +A ++ NP+ H R KHIE+ H +R+ +QKG++ L
Sbjct: 4 WLTYLLQDLKVPFEQPALVYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGLIHL 183
Query: 995 KFVDTDHQWADIFTKPLAEDRFNFILKNLNM 1025
+ + ADI+TK L+ F+ I L +
Sbjct: 184 LPISSSEPLADIYTKALSPQNFHQICAKLGL 276
>BP043850
Length = 515
Score = 70.9 bits (172), Expect = 1e-12
Identities = 34/93 (36%), Positives = 55/93 (58%), Gaps = 1/93 (1%)
Frame = -1
Query: 932 TQMLWMKH*LEDYQILESN-IPIYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYVQKG 990
++++W++ L + L+S P++ DNT+AI ++ NP+ H +HIEV H +R+ +
Sbjct: 515 SEIIWLRGLLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYDRR 336
Query: 991 VLLLKFVDTDHQWADIFTKPLAEDRFNFILKNL 1023
V+ L V T Q ADI TK L R NF++ L
Sbjct: 335 VITLPHVSTSVQIADILTKSLTRQRHNFLVSKL 237
>AV427921
Length = 284
Score = 67.0 bits (162), Expect = 1e-11
Identities = 33/79 (41%), Positives = 45/79 (56%)
Frame = +1
Query: 299 TPQQNGVVERKNRTLQEMARTMLQETGMAKHFWAEAVNTACYIQNRISVRPILNKTPYEL 358
TPQQNGV ERKNRT+ M R++L +G+ K F EAV + +I NR + N P E
Sbjct: 22 TPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQNMMPEEA 201
Query: 359 WKNIKPNISYFHPFGCVCY 377
W +P + +F C+ Y
Sbjct: 202 WSGRQPAVDHFRISRCLAY 258
>BP063694
Length = 511
Score = 66.2 bits (160), Expect = 2e-11
Identities = 44/122 (36%), Positives = 60/122 (49%), Gaps = 3/122 (2%)
Frame = -2
Query: 141 VWHRRLGHASMRKISQLSK---LNLVRGLPNLKFASDALCEACQKDKFTKVPFKAKNVVS 197
+WH RLGH + I QL K L++ LP C +CQ K ++ F N
Sbjct: 360 LWHSRLGHVNFDIIKQLHKHGCLDVSSILPK-----PICCTSCQMAKSKRLVFHDNNK-R 199
Query: 198 TSRSLELLHIDLFGPVKTESIGGKRYGMVIVDDYSRWTWVKFLTRKDESHAVFSTFIAQV 257
S L+L+H DL GP SI G Y ++ VDD+SR+TW L RK + V F +
Sbjct: 198 ASAVLDLIHCDLRGPSPVASIDGFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLLRFKVFM 19
Query: 258 QN 259
+N
Sbjct: 18 EN 13
>TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprotein, partial
(4%)
Length = 528
Score = 49.7 bits (117), Expect(2) = 5e-11
Identities = 26/51 (50%), Positives = 32/51 (61%)
Frame = +3
Query: 975 HIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFNFILKNLNM 1025
+IE KYHF+RD V KG + LK TD Q ADI TK L +RF + LN+
Sbjct: 69 NIETKYHFLRDQVTKGKISLKHCGTDLQVADIMTKGLKTERFRNMRAMLNV 221
Score = 35.4 bits (80), Expect(2) = 5e-11
Identities = 14/22 (63%), Positives = 18/22 (81%)
Frame = +2
Query: 956 DNTAAISLSKNPILHSRAKHIE 977
DN +AI L+KNP+ H R+KHIE
Sbjct: 5 DNKSAIDLAKNPVSHGRSKHIE 70
>AU088980
Length = 360
Score = 62.8 bits (151), Expect = 3e-10
Identities = 29/105 (27%), Positives = 51/105 (47%)
Frame = +2
Query: 303 NGVVERKNRTLQEMARTMLQETGMAKHFWAEAVNTACYIQNRISVRPILNKTPYELWKNI 362
N +VERK+R + + R ++ + + K+ W AV A Y+ N + + + PY+ N
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAYLINXLPSPLLKGQCPYQFLNND 181
Query: 363 KPNISYFHPFGCVCYVLNTKDRLHKFDAKSSKCLLLGYSERSKGF 407
P + FG +CY K D ++ KC+ LG+ + G+
Sbjct: 182 SPTLLDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKTGTXGY 316
>AV766665
Length = 601
Score = 62.4 bits (150), Expect = 4e-10
Identities = 32/76 (42%), Positives = 44/76 (57%)
Frame = +3
Query: 848 TAVKRILRYLKGTTNLGLMYKKTSEYKLSGYCDADYAGDRTERKSTSGNCQFLGSNLVSW 907
T RI YLK + G +++K + + G+ ADY G +R ST G FL NLV+W
Sbjct: 360 TFTTRIF*YLKANSRRGPLFQKEGKSSMDGFTYADYLGSIVDRLSTMGYYMFLSGNLVTW 539
Query: 908 ASKRQSTIALSTAEAE 923
SK+Q+ IA S+ EAE
Sbjct: 540 RSKQQNIIARSSGEAE 587
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 60.1 bits (144), Expect = 2e-09
Identities = 37/145 (25%), Positives = 71/145 (48%), Gaps = 9/145 (6%)
Frame = +2
Query: 685 SFLLENEFERGKVDTTLFCKTYKD-DILIVQIYVDDIIFGSANQSLCKEFYEMMQAEFEM 743
SF++ + R D + K + D D +I+ +YVDD++ N+ +E + EF+M
Sbjct: 8 SFIMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREFDM 187
Query: 744 SMMGELKYFLGIQV--DQTPEGTYIHQSKYTKELLKKFNMLESTVAKTP------MHPTC 795
+G LG+Q+ D+ ++ Q Y +++L++FNM + TP + +
Sbjct: 188 KDLGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSSSM 367
Query: 796 ILEKEDKSGKVCQKLYRGTIGSLLY 820
I E + ++ + Y +GSL+Y
Sbjct: 368 IPSSEAERMEMSRVPYASAVGSLMY 442
>TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, partial (4%)
Length = 670
Score = 56.2 bits (134), Expect = 3e-08
Identities = 27/91 (29%), Positives = 51/91 (55%), Gaps = 1/91 (1%)
Frame = +1
Query: 936 WMKH*LEDYQI-LESNIPIYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYVQKGVLLL 994
W+ + L+D ++ S +YCD+ +A ++ N + H R KH+++ H +R+ +Q + L
Sbjct: 13 WLTYILQDLRVPFISPSLLYCDSQSARHIATNAVFHERTKHLDIDCHVVREKLQAKLFHL 192
Query: 995 KFVDTDHQWADIFTKPLAEDRFNFILKNLNM 1025
+ + Q ADI TKPL F+ ++ L +
Sbjct: 193 LPISSVDQTADILTKPLESGPFSHLVSKLGV 285
>TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%)
Length = 550
Score = 56.2 bits (134), Expect = 3e-08
Identities = 29/82 (35%), Positives = 48/82 (58%), Gaps = 1/82 (1%)
Frame = +3
Query: 933 QMLWMKH*LEDYQILESN-IPIYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYVQKGV 991
+ LW+ + L D +I + IYCDN +A+ L+ N + H R ++IE+ H + V G+
Sbjct: 21 EALWLTYALADLRIASLLLVVIYCDNRSALHLAANSVFHKRTENIEIDCHIV*VKVLFGI 200
Query: 992 LLLKFVDTDHQWADIFTKPLAE 1013
L L V + Q AD+FTK +++
Sbjct: 201 LHLLHVPSSDQVADVFTKTISQ 266
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 51.6 bits (122), Expect = 6e-07
Identities = 35/114 (30%), Positives = 59/114 (51%), Gaps = 3/114 (2%)
Frame = +3
Query: 905 VSWASKRQSTIALSTAEAEYISAAICSTQMLWMKH*LEDYQILESNIPIYC---DNTAAI 961
V+W S+ Q +ALSTAEAE+I+A ++LWMK+ L++ + PI C A
Sbjct: 27 VAWPSRLQKCVALSTAEAEFIAATEACHELLWMKNFLQNAWF--HSHPILCCIVITKALF 200
Query: 962 SLSKNPILHSRAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDR 1015
+L++ + + +RD + +L L+ + TD AD+ TK L ++
Sbjct: 201 TLARILLFIQDPSTLMFVIIGLRDVLNSKLLELEKIHTDDDGADMMTKSLPREK 362
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 51.6 bits (122), Expect = 6e-07
Identities = 36/99 (36%), Positives = 48/99 (48%), Gaps = 1/99 (1%)
Frame = -1
Query: 768 QSKYTKELLKKFNMLESTVAKTPMHPTCILEKEDKSGK-VCQKLYRGTIGSLLYLTASRP 826
Q Y ++LKKF M S T + I GK V Y+ IGS+ YL R
Sbjct: 739 QKIYVDDILKKFKMTNSKYISTTIGGKEIEAGRRNGGKRVDSTYYKSLIGSVRYLNTVRS 560
Query: 827 DILFSVHLCARF*SDPRETHLTAVKRILRYLKGTTNLGL 865
DI+ V L +RF +P + H +R LRY+KGT G+
Sbjct: 559 DIVCGVGLRSRF-MEP*DCH*QGAQRSLRYIKGTLKDGI 446
>AV424544
Length = 276
Score = 48.9 bits (115), Expect = 4e-06
Identities = 26/90 (28%), Positives = 48/90 (52%)
Frame = +3
Query: 682 RLSSFLLENEFERGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANQSLCKEFYEMMQAEF 741
+LSS+L + + D +LF K ++ +YVDD+I + + + + +F
Sbjct: 6 KLSSYLHILGYIQSAHDHSLFTKFRDASFTVILVYVDDLILAGNDLNEIQCVKNKLDIQF 185
Query: 742 EMSMMGELKYFLGIQVDQTPEGTYIHQSKY 771
+ +G LKYFLG++V ++ G ++ Q KY
Sbjct: 186 RIKDLGTLKYFLGLEVARSSCGLFLSQRKY 275
>TC19474 weakly similar to UP|Q9FJA1 (Q9FJA1) Similarity to retroelement pol
polyprotein, partial (3%)
Length = 517
Score = 37.0 bits (84), Expect(2) = 1e-05
Identities = 21/54 (38%), Positives = 32/54 (58%)
Frame = -2
Query: 956 DNTAAISLSKNPILHSRAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTK 1009
DN AA+ + K RAKHIE+ +HF+ D + +FV+++ Q D+FTK
Sbjct: 354 DNKAALHMPKI*F-SMRAKHIEIDFHFL*DRRLYLDISTRFVNSNDQLTDVFTK 196
Score = 29.6 bits (65), Expect(2) = 1e-05
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = -1
Query: 900 LGSNLVSWASKRQSTIALSTAEAEY 924
L NL+SW SK +A S AEAE+
Sbjct: 517 LSDNLISWKSKETIIVARSRAEAEF 443
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.136 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,474,288
Number of Sequences: 28460
Number of extensions: 237045
Number of successful extensions: 1215
Number of sequences better than 10.0: 69
Number of HSP's better than 10.0 without gapping: 1190
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1204
length of query: 1032
length of database: 4,897,600
effective HSP length: 99
effective length of query: 933
effective length of database: 2,080,060
effective search space: 1940695980
effective search space used: 1940695980
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0177.6