BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0177.1
(651 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9282 similar to UP|Q94LX7 (Q94LX7) Phosphoenolpyruvate carboxy... 610 e-175
TC16830 similar to UP|Q9SDP6 (Q9SDP6) ATP-dependent phosphoenolp... 307 3e-84
AV417904 270 5e-73
AV409648 266 6e-72
TC18110 similar to UP|PPCK_ARATH (Q9T074) Phosphoenolpyruvate ca... 133 8e-32
AV764664 95 4e-20
AV781250 74 7e-14
BE122577 48 9e-11
TC10661 29 2.1
TC11672 similar to UP|Q9NGW5 (Q9NGW5) Brakeless protein, partial... 28 3.5
BP035375 28 4.6
AV424228 28 6.0
TC13454 homologue to UP|O64910 (O64910) Glucose-6-phosphate/phos... 28 6.0
TC15775 similar to UP|WR17_ARATH (Q9SJA8) Probable WRKY transcri... 28 6.0
BP054955 28 6.0
CB829171 28 6.0
>TC9282 similar to UP|Q94LX7 (Q94LX7) Phosphoenolpyruvate carboxykinase ,
partial (44%)
Length = 1236
Score = 610 bits (1572), Expect = e-175
Identities = 294/294 (100%), Positives = 294/294 (100%)
Frame = +3
Query: 358 STDHNRYLIGDDEHCWSDKGVSNIEGGCYAKCIDLSREKEPDIWNAIRFGTVLENVVFDE 417
STDHNRYLIGDDEHCWSDKGVSNIEGGCYAKCIDLSREKEPDIWNAIRFGTVLENVVFDE
Sbjct: 3 STDHNRYLIGDDEHCWSDKGVSNIEGGCYAKCIDLSREKEPDIWNAIRFGTVLENVVFDE 182
Query: 418 HTREVDYSDKSVTENTRAAYPIEYIPNAKLPCVGPHPTNVILLACDAFGVLPPVSKLNLA 477
HTREVDYSDKSVTENTRAAYPIEYIPNAKLPCVGPHPTNVILLACDAFGVLPPVSKLNLA
Sbjct: 183 HTREVDYSDKSVTENTRAAYPIEYIPNAKLPCVGPHPTNVILLACDAFGVLPPVSKLNLA 362
Query: 478 QTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAEKMEQHGATGW 537
QTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAEKMEQHGATGW
Sbjct: 363 QTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAEKMEQHGATGW 542
Query: 538 LVNTGWSGGSYGSGSRIKLQYTRKIIDAIHSGSLLKAEFQKTPIFGLEIPTEVEGVPSEI 597
LVNTGWSGGSYGSGSRIKLQYTRKIIDAIHSGSLLKAEFQKTPIFGLEIPTEVEGVPSEI
Sbjct: 543 LVNTGWSGGSYGSGSRIKLQYTRKIIDAIHSGSLLKAEFQKTPIFGLEIPTEVEGVPSEI 722
Query: 598 LDPVNTWSDKDAYQETLLKLAGLFKNNFETFTNYKIGKGDNNLTEEILAAGPNF 651
LDPVNTWSDKDAYQETLLKLAGLFKNNFETFTNYKIGKGDNNLTEEILAAGPNF
Sbjct: 723 LDPVNTWSDKDAYQETLLKLAGLFKNNFETFTNYKIGKGDNNLTEEILAAGPNF 884
>TC16830 similar to UP|Q9SDP6 (Q9SDP6) ATP-dependent phosphoenolpyruvate
carboxykinase (Fragment), partial (29%)
Length = 565
Score = 307 bits (787), Expect = 3e-84
Identities = 152/152 (100%), Positives = 152/152 (100%)
Frame = +2
Query: 1 MGEENGNGIATNGLPSIHTQKNGICHDDSVPTVKANTIDELHSLQKKKSAPGTPISGTQT 60
MGEENGNGIATNGLPSIHTQKNGICHDDSVPTVKANTIDELHSLQKKKSAPGTPISGTQT
Sbjct: 110 MGEENGNGIATNGLPSIHTQKNGICHDDSVPTVKANTIDELHSLQKKKSAPGTPISGTQT 289
Query: 61 PFTSDPERQQQQLQSISASLASLTRETGPKVVKGDPAKKLENPKTIHQVSHHHIAPTIAV 120
PFTSDPERQQQQLQSISASLASLTRETGPKVVKGDPAKKLENPKTIHQVSHHHIAPTIAV
Sbjct: 290 PFTSDPERQQQQLQSISASLASLTRETGPKVVKGDPAKKLENPKTIHQVSHHHIAPTIAV 469
Query: 121 SDSALKFTHVLYNLSPAELYEQAIRYEKGSFV 152
SDSALKFTHVLYNLSPAELYEQAIRYEKGSFV
Sbjct: 470 SDSALKFTHVLYNLSPAELYEQAIRYEKGSFV 565
>AV417904
Length = 413
Score = 270 bits (690), Expect = 5e-73
Identities = 123/136 (90%), Positives = 129/136 (94%)
Frame = +2
Query: 321 YLMPKRKILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCWSDKGVSN 380
YLMPKR ILSLHSGCNMGK+GDVA FFGLSGTGKTTLSTDHNRYLIGDDEHCWS+ GVSN
Sbjct: 5 YLMPKRNILSLHSGCNMGKNGDVAFFFGLSGTGKTTLSTDHNRYLIGDDEHCWSENGVSN 184
Query: 381 IEGGCYAKCIDLSREKEPDIWNAIRFGTVLENVVFDEHTREVDYSDKSVTENTRAAYPIE 440
IEGGCYAKC+DLSR+KEPDIWNAI+FGTVLENVVFDEH REVDYSDKSVTENTRAAYPIE
Sbjct: 185 IEGGCYAKCVDLSRDKEPDIWNAIKFGTVLENVVFDEHAREVDYSDKSVTENTRAAYPIE 364
Query: 441 YIPNAKLPCVGPHPTN 456
YIPNAK+PCV PH N
Sbjct: 365 YIPNAKIPCVAPHAKN 412
>AV409648
Length = 383
Score = 266 bits (681), Expect = 6e-72
Identities = 126/127 (99%), Positives = 127/127 (99%)
Frame = +1
Query: 241 RAYHSLFMHNMCIRPTPEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSVDLNLARREM 300
+AYHSLFMHNMCIRPTPEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSVDLNLARREM
Sbjct: 1 QAYHSLFMHNMCIRPTPEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSVDLNLARREM 180
Query: 301 VILGTQYAGEMKKGLFGVMHYLMPKRKILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTD 360
VILGTQYAGEMKKGLFGVMHYLMPKRKILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTD
Sbjct: 181 VILGTQYAGEMKKGLFGVMHYLMPKRKILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTD 360
Query: 361 HNRYLIG 367
HNRYLIG
Sbjct: 361 HNRYLIG 381
>TC18110 similar to UP|PPCK_ARATH (Q9T074) Phosphoenolpyruvate carboxykinase
[ATP] (PEP carboxykinase) (Phosphoenolpyruvate
carboxylase) (PEPCK) , partial (13%)
Length = 586
Score = 133 bits (335), Expect = 8e-32
Identities = 65/90 (72%), Positives = 76/90 (84%)
Frame = +1
Query: 562 IIDAIHSGSLLKAEFQKTPIFGLEIPTEVEGVPSEILDPVNTWSDKDAYQETLLKLAGLF 621
IIDAIHSGSLL AE+ KT +FGLEIP E++GVPSEILDP+NTWSDK+A+++ LLKLA LF
Sbjct: 1 IIDAIHSGSLLNAEYTKTEVFGLEIPKEIDGVPSEILDPINTWSDKNAHKDALLKLASLF 180
Query: 622 KNNFETFTNYKIGKGDNNLTEEILAAGPNF 651
K NF+ FT YKIG D LTEEI+AAGP F
Sbjct: 181 KKNFDVFTAYKIG-DDQKLTEEIVAAGPIF 267
>AV764664
Length = 264
Score = 94.7 bits (234), Expect = 4e-20
Identities = 47/70 (67%), Positives = 55/70 (78%)
Frame = -1
Query: 582 FGLEIPTEVEGVPSEILDPVNTWSDKDAYQETLLKLAGLFKNNFETFTNYKIGKGDNNLT 641
FGLEIPT +EGVPS+I DPV TWS K+A+ ETL KLAGLFKNNF TF KIGKG +
Sbjct: 264 FGLEIPT*MEGVPSKIFDPVKTWSGKNAFPETLFKLAGLFKNNF*TFPPPKIGKGGPHPP 85
Query: 642 EEILAAGPNF 651
++I AAGP+F
Sbjct: 84 KKIFAAGPHF 55
>AV781250
Length = 463
Score = 73.9 bits (180), Expect = 7e-14
Identities = 38/63 (60%), Positives = 51/63 (80%), Gaps = 2/63 (3%)
Frame = -3
Query: 590 VEGVPSEILDPVNTWSDKDAYQETLLKLAGLFKNNFETFTNY-KIGKGDNNLTEEILA-A 647
VEGVPSE LDPV+TWSD DAY ETLLKLAGL +++FETFT ++G+GD++ ++ + A
Sbjct: 461 VEGVPSEFLDPVHTWSDPDAYPETLLKLAGLVQDHFETFTLLPRLGQGDHHPPQKRFSPA 282
Query: 648 GPN 650
GP+
Sbjct: 281 GPH 273
>BE122577
Length = 151
Score = 48.1 bits (113), Expect(2) = 9e-11
Identities = 23/28 (82%), Positives = 25/28 (89%)
Frame = +1
Query: 154 STGALATLSGAKTGRSPRDKRVVRDAVT 181
+TGALATLSGAK GRSPRDKRVV+D T
Sbjct: 16 ATGALATLSGAKIGRSPRDKRVVKDDTT 99
Score = 35.4 bits (80), Expect(2) = 9e-11
Identities = 13/13 (100%), Positives = 13/13 (100%)
Frame = +3
Query: 185 LWWGKGSPNIEMD 197
LWWGKGSPNIEMD
Sbjct: 111 LWWGKGSPNIEMD 149
>TC10661
Length = 1060
Score = 29.3 bits (64), Expect = 2.1
Identities = 14/28 (50%), Positives = 15/28 (53%)
Frame = -1
Query: 346 FFGLSGTGKTTLSTDHNRYLIGDDEHCW 373
F GK +L T HN YLIGD CW
Sbjct: 847 FLSSGEVGKPSLRTVHNSYLIGD---CW 773
>TC11672 similar to UP|Q9NGW5 (Q9NGW5) Brakeless protein, partial (3%)
Length = 555
Score = 28.5 bits (62), Expect = 3.5
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = +2
Query: 49 SAPGTPISGTQTPFTSDPERQ 69
++PG P SG + P SDP+RQ
Sbjct: 272 ASPGLPWSGKRWPLVSDPQRQ 334
>BP035375
Length = 543
Score = 28.1 bits (61), Expect = 4.6
Identities = 10/25 (40%), Positives = 17/25 (68%)
Frame = -2
Query: 541 TGWSGGSYGSGSRIKLQYTRKIIDA 565
+GW GG+Y + I + TRK++D+
Sbjct: 221 SGWCGGAYSAWYSISFRRTRKMLDS 147
>AV424228
Length = 323
Score = 27.7 bits (60), Expect = 6.0
Identities = 14/29 (48%), Positives = 16/29 (54%)
Frame = -1
Query: 147 EKGSFVTSTGALATLSGAKTGRSPRDKRV 175
E G FV STG S T + PRD+RV
Sbjct: 284 EGGHFVRSTGRKDRHSKVHTSKGPRDRRV 198
>TC13454 homologue to UP|O64910 (O64910)
Glucose-6-phosphate/phosphate-translocator precursor,
partial (56%)
Length = 674
Score = 27.7 bits (60), Expect = 6.0
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +2
Query: 113 HIAPTIAVSDSALKFTHVLYNLSPA 137
H+A T+++S A+ FTH++ + PA
Sbjct: 98 HVAATVSMSKVAVSFTHIIKSGEPA 172
>TC15775 similar to UP|WR17_ARATH (Q9SJA8) Probable WRKY transcription
factor 17 (WRKY DNA-binding protein 17), partial (53%)
Length = 1284
Score = 27.7 bits (60), Expect = 6.0
Identities = 31/107 (28%), Positives = 44/107 (40%), Gaps = 5/107 (4%)
Frame = +3
Query: 66 PERQQQQLQSISAS----LASLTRETGPKVVKGDPAKKLENPKTIHQVSHHHIAPTIAVS 121
P Q L ++ S L SL TG + P P + ++ HH PT+A S
Sbjct: 189 PSHLQTDLTDVTVSNFKKLISLLNRTGHARFRRAPLPSTPTPPQPNPIAVHHPKPTVAQS 368
Query: 122 DSALKFTHV-LYNLSPAELYEQAIRYEKGSFVTSTGALATLSGAKTG 167
L FT + S A+ E E +F S+ + + LS A TG
Sbjct: 369 -LTLDFTKPNNFLTSNAKSLELEFSKETTTFSVSSNS-SFLSSAITG 503
>BP054955
Length = 525
Score = 27.7 bits (60), Expect = 6.0
Identities = 16/58 (27%), Positives = 27/58 (45%)
Frame = +3
Query: 513 FIMLHPTKYAAMLAEKMEQHGATGWLVNTGWSGGSYGSGSRIKLQYTRKIIDAIHSGS 570
F++ K ++A+ + G GW G GS G + +YT+K + +HS S
Sbjct: 21 FLLQIRGKCPRVIAQVVRARGHMGW---EGEVQGSIFEGCNLSFRYTKKKEEIVHSSS 185
>CB829171
Length = 487
Score = 27.7 bits (60), Expect = 6.0
Identities = 11/29 (37%), Positives = 16/29 (54%)
Frame = -2
Query: 517 HPTKYAAMLAEKMEQHGATGWLVNTGWSG 545
HP + A +++ G G L NTGW+G
Sbjct: 132 HPNRLIASNYSRLDCKGILGCLQNTGWNG 46
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,945,620
Number of Sequences: 28460
Number of extensions: 144973
Number of successful extensions: 747
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 743
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 746
length of query: 651
length of database: 4,897,600
effective HSP length: 96
effective length of query: 555
effective length of database: 2,165,440
effective search space: 1201819200
effective search space used: 1201819200
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0177.1