BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0176a.11
(597 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC13387 92 3e-19
BP071119 89 2e-18
TC19261 similar to UP|BAD06577 (BAD06577) Cell wall protein Awa1... 60 1e-09
AV780545 53 1e-07
BP046302 52 4e-07
BP067482 50 1e-06
TC8142 UP|BAD11133 (BAD11133) Glutamate-rich protein, complete 45 4e-05
TC7812 UP|Q9LKJ6 (Q9LKJ6) Water-selective transport intrinsic me... 43 2e-04
TC15784 similar to UP|PE31_ARATH (Q9LHA7) Peroxidase 31 precurso... 42 3e-04
TC19044 similar to UP|Q9LJH9 (Q9LJH9) Gb|AAD25781.1, partial (16%) 32 6e-04
BF177554 40 0.001
TC15802 similar to UP|Q9XIV1 (Q9XIV1) mRNA expressed in cucumber... 40 0.001
TC15253 weakly similar to UP|Q9ZT16 (Q9ZT16) Arabinogalactan-pro... 37 0.007
AV769506 37 0.007
TC8089 similar to UP|FLA1_ARATH (Q9FM65) Fasciclin-like arabinog... 37 0.012
TC16241 weakly similar to UP|Q91TH4 (Q91TH4) T122, partial (7%) 35 0.035
TC14875 similar to UP|Q9VNX6 (Q9VNX6) CG7421-PA, partial (4%) 35 0.035
TC11037 weakly similar to GB|AAP12885.1|30017303|BT006236 At3g12... 35 0.035
AV421977 34 0.059
AV411518 34 0.059
>TC13387
Length = 511
Score = 91.7 bits (226), Expect = 3e-19
Identities = 42/75 (56%), Positives = 48/75 (64%)
Frame = +1
Query: 507 YTYLPMRGGDPPLEHPVIAIGHVTNHFVQLKLVSGHPMPPIAPQWEYNVEEPESEWCKPY 566
++YLP+RG PP H VIAIGHV NHFVQL L GH MPPIA QWE V+ WC PY
Sbjct: 94 WSYLPLRGEGPPPVHRVIAIGHVINHFVQLHLTLGHSMPPIALQWERYVDPISVSWCVPY 273
Query: 567 KERLDRFMAEHTVWI 581
L RF +E W+
Sbjct: 274 VTHLHRFTSEFEAWL 318
>BP071119
Length = 412
Score = 89.4 bits (220), Expect = 2e-18
Identities = 49/117 (41%), Positives = 68/117 (57%)
Frame = +2
Query: 168 FPGGPVELSLLQHYPDHIAPWTWHTLLGTTDPRYSERGDLRLATAGTKLGLMTCEGDNYR 227
+ GGP +L LL Y +HIA W + P Y RG L++ G + + R
Sbjct: 80 YEGGPFDLCLLTKYGEHIARDIWRSY---KRPNYETRGVLKIYNHGRMCHPVV---HHAR 241
Query: 228 EVRLIVERSGLYPLVRCSYVETDPGLISALVERWHEETSSFHMPFGEMTVTLDDVSA 284
+R V +GL +++C+ D +ISA VERWH ETSSFH+P+GEMT+TL+DVSA
Sbjct: 242 YIRGAVHNAGLRWVMKCTSPSVDQSIISAFVERWHPETSSFHLPWGEMTITLEDVSA 412
>TC19261 similar to UP|BAD06577 (BAD06577) Cell wall protein Awa1p, partial
(3%)
Length = 495
Score = 59.7 bits (143), Expect = 1e-09
Identities = 31/67 (46%), Positives = 43/67 (63%)
Frame = -1
Query: 302 RYDVAETCALLLGGDADLYMAEFDKLRGPTMRFSFLRDLYPKAVAEGRYEHAARMYLMHL 361
R +VA CA LLGG Y+AEF+ G T+RF L+ + A+ GRYE AAR++L++
Sbjct: 489 RDEVAALCAQLLGGSVAAYLAEFESAGGQTLRFLTLKTMSTSAMDGGRYEDAARIWLVNP 310
Query: 362 VGATLFA 368
+ ATL A
Sbjct: 309 LAATLVA 289
>AV780545
Length = 555
Score = 53.1 bits (126), Expect = 1e-07
Identities = 41/109 (37%), Positives = 53/109 (48%)
Frame = +1
Query: 171 GPVELSLLQHYPDHIAPWTWHTLLGTTDPRYSERGDLRLATAGTKLGLMTCEGDNYREVR 230
GP++ S+L H++ T +G ERG LR +KL T V
Sbjct: 265 GPMDGSVLYDQDKHVS-----TAVGEGQ---QERGALRCHEHTSKLDQWTLTPKQME*V- 417
Query: 231 LIVERSGLYPLVRCSYVETDPGLISALVERWHEETSSFHMPFGEMTVTL 279
ER+G L + D LISALVERW ET++FH+ GEMTVTL
Sbjct: 418 ---ERAGFGYLRSIPAISLDNPLISALVERWRRETNTFHLNVGEMTVTL 555
>BP046302
Length = 435
Score = 51.6 bits (122), Expect = 4e-07
Identities = 23/46 (50%), Positives = 26/46 (56%)
Frame = -3
Query: 535 QLKLVSGHPMPPIAPQWEYNVEEPESEWCKPYKERLDRFMAEHTVW 580
+L L GH MPPIA QWE V+ WC PY RL RF +E W
Sbjct: 295 ELHLTPGHSMPPIALQWERYVDPISVSWCVPYVTRLHRFTSELEAW 158
>BP067482
Length = 291
Score = 49.7 bits (117), Expect = 1e-06
Identities = 30/81 (37%), Positives = 42/81 (51%), Gaps = 1/81 (1%)
Frame = +2
Query: 292 GRFYTPGVASRYDVAETCALLLGGDADLYM-AEFDKLRGPTMRFSFLRDLYPKAVAEGRY 350
G F++ G +R + A A LL D + EFD RGP++RFSFL+ + V
Sbjct: 17 GEFFSFGNPTREEAAPAVAQLLDVDIETI**EEFDACRGPSLRFSFLQAIVETHVEANNE 196
Query: 351 EHAARMYLMHLVGATLFADKS 371
A R Y++ +G TL DKS
Sbjct: 197 VPACRAYMLRWIGMTLVCDKS 259
>TC8142 UP|BAD11133 (BAD11133) Glutamate-rich protein, complete
Length = 999
Score = 44.7 bits (104), Expect = 4e-05
Identities = 39/133 (29%), Positives = 59/133 (44%), Gaps = 15/133 (11%)
Frame = +1
Query: 58 PAPATEVPATQVPDTEVPAPATEVPATQVPATEVPALSTPEVTATEVSPQSTPEVTAHDT 117
P T++ T +P+ E PA ATEV A + PA EVPA + P + P+ T +T
Sbjct: 142 PTEVTKIQET-IPEPEQPA-ATEVAAPEQPAAEVPAPAEPATEEPKEETTEEPKETETET 315
Query: 118 ---VEPSTSS-LDIDAVE----------EPESESGSESGSESEIEGEDVEVEDPNMPPLQ 163
V P T L+++ E +PE+E E E + E E + P++
Sbjct: 316 EAPVAPETQDPLEVENKEVTEEAKPEEVKPETEKTEEKTEEPKTEEPAATTETESAAPVE 495
Query: 164 RD-PPFPGGPVEL 175
+ P PVE+
Sbjct: 496 EENKPAEEAPVEV 534
>TC7812 UP|Q9LKJ6 (Q9LKJ6) Water-selective transport intrinsic membrane
protein 1, complete
Length = 1149
Score = 42.7 bits (99), Expect = 2e-04
Identities = 32/100 (32%), Positives = 44/100 (44%), Gaps = 6/100 (6%)
Frame = +1
Query: 50 APKATTEVPAPATEVPATQVPDTEVPAPATEVP---ATQVPAT--EVPALSTPEVTATEV 104
+P +++ PAP E P T P T P P P T P++ A ++P T+T
Sbjct: 157 SPPSSSSSPAPVPESPTTNSPTTAPPPPPASSPPPSPTHSPSSSPSPSAPTSPAATSTPP 336
Query: 105 SPQ-STPEVTAHDTVEPSTSSLDIDAVEEPESESGSESGS 143
SP S+ VT+ + STSSL P S S S
Sbjct: 337 SPSASSSAVTSPSSAVSSTSSLSFSDPSSPPCSSPSSPDS 456
>TC15784 similar to UP|PE31_ARATH (Q9LHA7) Peroxidase 31 precursor (Atperox
P31) (ATP41) , partial (38%)
Length = 475
Score = 42.0 bits (97), Expect = 3e-04
Identities = 27/103 (26%), Positives = 42/103 (40%)
Frame = +1
Query: 25 SHRRGDHDTDVSTSRKRARKGARKGAPKATTEVPAPATEVPATQVPDTEVPAPATEVPAT 84
SH R H T +T + K + +P + P PA P A+
Sbjct: 94 SHTRDSHSTSTNTRAHNSPKSSATPSPTNKSPPPPPAP------------PRSASSSTTA 237
Query: 85 QVPATEVPALSTPEVTATEVSPQSTPEVTAHDTVEPSTSSLDI 127
P P S+P++ +T+ P +TP T+ PSTSS ++
Sbjct: 238 STPTAATPPSSSPQLPSTK--PNATPTSTSPSPATPSTSSSEL 360
>TC19044 similar to UP|Q9LJH9 (Q9LJH9) Gb|AAD25781.1, partial (16%)
Length = 620
Score = 32.0 bits (71), Expect(2) = 6e-04
Identities = 20/81 (24%), Positives = 32/81 (38%), Gaps = 9/81 (11%)
Frame = +3
Query: 38 SRKRARKGARKGAPKATTEVPAPATEVPATQV---PDTEVP------APATEVPATQVPA 88
S +K P + P P P T+ P + P + AT T P+
Sbjct: 171 STANVKKSISTSKPSTKSNAPCPPPPSPTTKTKPPPPSRSPWLA*KTSSATSSSPTPTPS 350
Query: 89 TEVPALSTPEVTATEVSPQST 109
T++P STP T+++ +T
Sbjct: 351 TQLPRFSTPTPKKTKINTTTT 413
Score = 28.1 bits (61), Expect(2) = 6e-04
Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 3/44 (6%)
Frame = +2
Query: 105 SPQSTPEVTAHDTVEPSTSSLDIDAVEEPE---SESGSESGSES 145
S STP+ HD V+ +D+D V++ + +SGS S S
Sbjct: 425 SSSSTPQRLHHDAVDDGGGDVDVDVVDDGKLLRFDSGSSVASSS 556
>BF177554
Length = 246
Score = 40.0 bits (92), Expect = 0.001
Identities = 27/73 (36%), Positives = 36/73 (48%), Gaps = 8/73 (10%)
Frame = +1
Query: 126 DIDAVEEPESESGSESGSESEIEGEDVEVEDPN------MPPLQRDPPFP--GGPVELSL 177
D D+ EE ES SE E E EGE+ E ED P + + F GGP ++SL
Sbjct: 25 DEDSCEEEAGES-SEEEEEEEEEGEEEEEEDDGPGDRDAQPEEEEEEDFSLLGGPYDISL 201
Query: 178 LQHYPDHIAPWTW 190
L+ + H+A W
Sbjct: 202 LKSFKGHVALRVW 240
>TC15802 similar to UP|Q9XIV1 (Q9XIV1) mRNA expressed in cucumber hypocotyls
(Arabinogalactan protein), partial (26%)
Length = 795
Score = 39.7 bits (91), Expect = 0.001
Identities = 34/136 (25%), Positives = 41/136 (30%), Gaps = 3/136 (2%)
Frame = +2
Query: 51 PKATTEVPAPATEVPATQVPDTEVPAPATEVPATQV---PATEVPALSTPEVTATEVSPQ 107
P A P PA PA PAPAT P T V P VP S P SP
Sbjct: 173 PVAPVASPKPAATTPAASPSTATAPAPATTTPVTPVTSPPPAAVPVASPPPAAVPVSSPP 352
Query: 108 STPEVTAHDTVEPSTSSLDIDAVEEPESESGSESGSESEIEGEDVEVEDPNMPPLQRDPP 167
+ T P + + V P + + P P+ D P
Sbjct: 353 AKSPPAPAPTAVPVAAPVTTPEVPAPAPSKTKKDAA-------------PAPSPVVPDSP 493
Query: 168 FPGGPVELSLLQHYPD 183
G P + PD
Sbjct: 494 PAGAPGPSDAVSPGPD 541
>TC15253 weakly similar to UP|Q9ZT16 (Q9ZT16) Arabinogalactan-protein
(ATAGP4) (AT5G10430/F12B17_220), partial (42%)
Length = 575
Score = 37.4 bits (85), Expect = 0.007
Identities = 29/89 (32%), Positives = 34/89 (37%)
Frame = +3
Query: 54 TTEVPAPATEVPATQVPDTEVPAPATEVPATQVPATEVPALSTPEVTATEVSPQSTPEVT 113
TT P P PA P PAT PA T P +TP +A+ +P T T
Sbjct: 180 TTTPPPPPAAAPA--------PPPATPPPAATPAPTTTPPAATPAPSASPPAPTPTASPT 335
Query: 114 AHDTVEPSTSSLDIDAVEEPESESGSESG 142
T PS SS P S G +G
Sbjct: 336 GAPT--PSASSPPAPIPSGPASGPGPAAG 416
Score = 35.0 bits (79), Expect = 0.035
Identities = 24/92 (26%), Positives = 34/92 (36%), Gaps = 14/92 (15%)
Frame = +3
Query: 51 PKATTEVPAPATEVPA-----TQVPDTEVPAPATEVPA---------TQVPATEVPALST 96
P A P PAT PA T P PAP+ PA P+ P
Sbjct: 198 PPAAAPAPPPATPPPAATPAPTTTPPAATPAPSASPPAPTPTASPTGAPTPSASSPPAPI 377
Query: 97 PEVTATEVSPQSTPEVTAHDTVEPSTSSLDID 128
P A+ P + P + DT P +++ ++
Sbjct: 378 PSGPASGPGPAAGPGPNSADTPPPPSAAFSLN 473
>AV769506
Length = 345
Score = 37.4 bits (85), Expect = 0.007
Identities = 17/41 (41%), Positives = 30/41 (72%), Gaps = 2/41 (4%)
Frame = -3
Query: 201 YSERGDLRLATAGTKLGLMTCEG--DNYREVRLIVERSGLY 239
Y +R L++ATAG K+ + C+G D++R VR ++E++GL+
Sbjct: 343 YVDRRQLKVATAGGKVWNLACDGDSDSHRRVRELIEQTGLH 221
>TC8089 similar to UP|FLA1_ARATH (Q9FM65) Fasciclin-like arabinogalactan
protein 1 precursor, partial (43%)
Length = 890
Score = 36.6 bits (83), Expect = 0.012
Identities = 24/97 (24%), Positives = 43/97 (43%), Gaps = 1/97 (1%)
Frame = +2
Query: 38 SRKRARKGARKGAPKATTEVPAPATEVPATQVPDTEVPAPATEVPATQVP-ATEVPALST 96
SR+R P+ T PA + P++ T P+P + +T P + P+ +
Sbjct: 179 SRQRCSSSPWLLPPQTPTTSPAFSPSTPSSPPSTTTSPSPTSPPRSTNGPQSPSAPSTTP 358
Query: 97 PEVTATEVSPQSTPEVTAHDTVEPSTSSLDIDAVEEP 133
P T+++ + QS P T+ + ST+S + P
Sbjct: 359 PWTTSSQNTHQSPPSRTSSPSTSSSTTSAPRSSTRSP 469
>TC16241 weakly similar to UP|Q91TH4 (Q91TH4) T122, partial (7%)
Length = 799
Score = 35.0 bits (79), Expect = 0.035
Identities = 23/67 (34%), Positives = 35/67 (51%), Gaps = 3/67 (4%)
Frame = +1
Query: 50 APKATTEVPA---PATEVPATQVPDTEVPAPATEVPATQVPATEVPALSTPEVTATEVSP 106
AP +T PA +T VPAT P ++ +P+ E P +PA P+ S+ +T+ +P
Sbjct: 307 APSISTSPPALTPASTPVPATASPPSQ--SPSAESPGASLPAATTPSPSSTTPRSTQ-TP 477
Query: 107 QSTPEVT 113
S E T
Sbjct: 478 SSNNETT 498
Score = 27.7 bits (60), Expect = 5.5
Identities = 17/63 (26%), Positives = 28/63 (43%), Gaps = 9/63 (14%)
Frame = +1
Query: 53 ATTEVPA--------PATEVPATQVPDTEVPAPATEVP-ATQVPATEVPALSTPEVTATE 103
A+T VPA P+ E P +P P+P++ P +TQ P++ A+
Sbjct: 346 ASTPVPATASPPSQSPSAESPGASLPAATTPSPSSTTPRSTQTPSSNNETTPAQRNGASS 525
Query: 104 VSP 106
+P
Sbjct: 526 ATP 534
>TC14875 similar to UP|Q9VNX6 (Q9VNX6) CG7421-PA, partial (4%)
Length = 1240
Score = 35.0 bits (79), Expect = 0.035
Identities = 21/69 (30%), Positives = 29/69 (41%), Gaps = 2/69 (2%)
Frame = +1
Query: 54 TTEVPAPATEVPATQVPDTEVPAPATEVPATQ--VPATEVPALSTPEVTATEVSPQSTPE 111
T +PAP P+ T P PA+ +T P T P+ ST + +P +T
Sbjct: 106 TPPLPAPTI*PPSNPKTSTSTPTPASPPASTSPLTPPTTSPSSSTSTAAPSASAPLTTTP 285
Query: 112 VTAHDTVEP 120
TA T P
Sbjct: 286 TTATSTTSP 312
>TC11037 weakly similar to GB|AAP12885.1|30017303|BT006236 At3g12920
{Arabidopsis thaliana;}, partial (27%)
Length = 730
Score = 35.0 bits (79), Expect = 0.035
Identities = 21/65 (32%), Positives = 32/65 (48%), Gaps = 1/65 (1%)
Frame = +2
Query: 54 TTEVPAPATEVPATQ-VPDTEVPAPATEVPATQVPATEVPALSTPEVTATEVSPQSTPEV 112
TT P PAT P+ + VP+T + T T+ PAT + S +T + ST
Sbjct: 281 TTPPPPPATVSPSRENVPETPLTTTTTSSLLTRTPATTLSPSSVKISPSTSTTTNSTSIT 460
Query: 113 TAHDT 117
++H+T
Sbjct: 461 SSHNT 475
>AV421977
Length = 535
Score = 34.3 bits (77), Expect = 0.059
Identities = 20/72 (27%), Positives = 33/72 (45%), Gaps = 5/72 (6%)
Frame = +2
Query: 75 PAPATEVPATQVPATEVPALSTPEVTATEVSPQSTPE-----VTAHDTVEPSTSSLDIDA 129
P P P++ P+T+ PA S P T S +TP V++ E ++S+ A
Sbjct: 239 PLPPPPPPSSTSPSTKTPAASPPPPTKASASTTATPSARFSAVSSAPAAESASSTCSSAA 418
Query: 130 VEEPESESGSES 141
P S + +E+
Sbjct: 419 TYSPSSAAAAEA 454
>AV411518
Length = 360
Score = 34.3 bits (77), Expect = 0.059
Identities = 19/57 (33%), Positives = 27/57 (47%), Gaps = 4/57 (7%)
Frame = +3
Query: 45 GARKGAPKATTEVPAP---ATEVPATQVPDTEVPAPATEV-PATQVPATEVPALSTP 97
G +P +T P P +T P + P T P P T P++ +P T P+ STP
Sbjct: 177 GPASPSPSSTAPSPRPNPSSTSGPTSPTPHTPSPPPPTSSSPSSAIPPTSAPSSSTP 347
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.134 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,580,192
Number of Sequences: 28460
Number of extensions: 162684
Number of successful extensions: 1233
Number of sequences better than 10.0: 123
Number of HSP's better than 10.0 without gapping: 1119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1190
length of query: 597
length of database: 4,897,600
effective HSP length: 95
effective length of query: 502
effective length of database: 2,193,900
effective search space: 1101337800
effective search space used: 1101337800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0176a.11