Lotus
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0175.3
         (520 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC10648                                                               213  8e-56
TC12061 similar to UP|Q9LN49 (Q9LN49) F18O14.21, partial (35%)         28  2.8
AV776901                                                               27  6.2
AV410595                                                               27  6.2
BP065974                                                               27  6.2
TC20075 similar to UP|Q9FG10 (Q9FG10) Ubiquitin carboxyl-termina...    27  8.1
TC12156 homologue to UP|Q93Y02 (Q93Y02) GTP-binding protein typA...    27  8.1
TC11700 homologue to GB|AAL06971.1|15810091|AY056083 AT4g30260/F...    27  8.1

>TC10648 
          Length = 419

 Score =  213 bits (541), Expect = 8e-56
 Identities = 108/126 (85%), Positives = 112/126 (88%)
 Frame = +3

Query: 395 DDRFPELLDPSDLEAEALADEILDLAESVKESLLQMTTRHMVSQAMADYPQAPHSDMVFI 454
           DD FPELLDPSDL+AEALADEILDL ESVKE+LLQMTTRHMVSQAMADYPQAPH DMVFI
Sbjct: 3   DDEFPELLDPSDLDAEALADEILDLEESVKETLLQMTTRHMVSQAMADYPQAPHPDMVFI 182

Query: 455 PLGMYLKTVSIFDCLRARAGRKFLSKQDRKTDYESLAHGDLQEVRHMLAMVVFDTIYPLH 514
            LGMYLK +SIF CLRARAGR FLSK   KTDYESLAHGDL EV HMLAMVVFD +YPL 
Sbjct: 183 SLGMYLKAISIFYCLRARAGRNFLSKPVIKTDYESLAHGDLPEVWHMLAMVVFDIVYPLP 362

Query: 515 LDRDQS 520
           LDRDQS
Sbjct: 363 LDRDQS 380


>TC12061 similar to UP|Q9LN49 (Q9LN49) F18O14.21, partial (35%)
          Length = 613

 Score = 28.5 bits (62), Expect = 2.8
 Identities = 12/43 (27%), Positives = 24/43 (54%)
 Frame = +1

Query: 137 PICHVHMDGTVVEVSSFNTNKFKPRTRFVHDIEAPNGCDNEDF 179
           P C   + G+ V +S+  ++KF+ + + VH +    G D++ F
Sbjct: 31  PNCLFRVGGSAVLLSNKASDKFRAKYKLVHVVRTHKGADDKAF 159


>AV776901 
          Length = 588

 Score = 27.3 bits (59), Expect = 6.2
 Identities = 11/37 (29%), Positives = 18/37 (47%)
 Frame = -2

Query: 26  RKVSQLKLNLNHTHHLASSFISVCFPQNKKTPAWKKL 62
           RK ++  +  ++TH   S +    +P     P WKKL
Sbjct: 509 RKSNKCTMQPSNTHQPTSKYSKAKYPTRYNNPIWKKL 399


>AV410595 
          Length = 225

 Score = 27.3 bits (59), Expect = 6.2
 Identities = 12/45 (26%), Positives = 24/45 (52%), Gaps = 12/45 (26%)
 Frame = -1

Query: 353 LHKTLSDQPR------------NPLVVAAFSLAVHNGGNLLEAVD 385
           LH TLS  PR            +P+++    +++H+G N+L +++
Sbjct: 180 LHPTLSHTPRAPSPNFIIKTNRHPIIITIIRISIHSGHNILLSIE 46


>BP065974 
          Length = 564

 Score = 27.3 bits (59), Expect = 6.2
 Identities = 21/69 (30%), Positives = 30/69 (43%)
 Frame = -1

Query: 275 MEMNYMLAYGSAEASLRLLWRFGLLDILLPFQAVYFVHHRFRRRDKRTNMLLSLFSNLDK 334
           ME+++   YG    SL       + DILL  Q V  VH   ++R         L  +L +
Sbjct: 429 MEISFPYLYGKEYLSLSSFQYLNMSDILLTLQGV--VHRLMKKR---------LMVSLIR 283

Query: 335 LLAPNRPCH 343
           +L   RP H
Sbjct: 282 MLGFMRPAH 256


>TC20075 similar to UP|Q9FG10 (Q9FG10) Ubiquitin carboxyl-terminal
           hydrolase, partial (8%)
          Length = 516

 Score = 26.9 bits (58), Expect = 8.1
 Identities = 10/29 (34%), Positives = 18/29 (61%)
 Frame = -1

Query: 132 VGKRFPICHVHMDGTVVEVSSFNTNKFKP 160
           +G+   IC++ + G +V + SF  NK+ P
Sbjct: 432 IGQTC*ICNIQIHGQIVHIVSFWENKYTP 346


>TC12156 homologue to UP|Q93Y02 (Q93Y02) GTP-binding protein typA (Tyrosine
           phosphorylated protein A), partial (30%)
          Length = 359

 Score = 26.9 bits (58), Expect = 8.1
 Identities = 15/40 (37%), Positives = 22/40 (54%)
 Frame = -1

Query: 49  CFPQNKKTPAWKKLNSKELGLRNSMIAHPTKRVLSTLKKK 88
           C PQ+KKT  W  +  K   LR++ +    K + S+L KK
Sbjct: 278 CIPQSKKTTGWDLIFEKCSSLRSNPL--HIKHLPSSLAKK 165


>TC11700 homologue to GB|AAL06971.1|15810091|AY056083 AT4g30260/F9N11_110
           {Arabidopsis thaliana;}, partial (45%)
          Length = 561

 Score = 26.9 bits (58), Expect = 8.1
 Identities = 20/75 (26%), Positives = 32/75 (42%), Gaps = 2/75 (2%)
 Frame = +1

Query: 16  HLRIAPFQCVRKVSQLKLNLNHTHHLAS--SFISVCFPQNKKTPAWKKLNSKELGLRNSM 73
           HLRI+  +  R+ +Q    L+  HHL S   F   C   ++        +   L    S 
Sbjct: 100 HLRISGHRPPRQTTQSSTRLHPRHHLFSLHQFQPPCSSSSQILHLPTSTSKTHLSPSCSS 279

Query: 74  IAHPTKRVLSTLKKK 88
             HPT+ +L  L+ +
Sbjct: 280 SLHPTRNLLLRLRTR 324


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.325    0.138    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,172,779
Number of Sequences: 28460
Number of extensions: 128487
Number of successful extensions: 798
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 795
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 798
length of query: 520
length of database: 4,897,600
effective HSP length: 94
effective length of query: 426
effective length of database: 2,222,360
effective search space: 946725360
effective search space used: 946725360
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 57 (26.6 bits)


Lotus: description of TM0175.3