BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0175.2
(314 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CB828026 135 9e-33
AV424133 128 1e-30
AV766794 95 1e-20
AW428702 92 1e-19
BP036382 86 8e-18
TC9193 similar to GB|AAN28890.1|23506061|AY143951 At4g02590/T10P... 75 1e-14
AV772495 75 2e-14
TC17246 similar to UP|Q8S490 (Q8S490) Transcription factor RAU1 ... 61 2e-10
TC18748 similar to GB|AAN28890.1|23506061|AY143951 At4g02590/T10... 61 3e-10
TC20174 similar to UP|O48535 (O48535) At2g42280 protein, partial... 53 8e-08
TC19009 similar to UP|AAQ14331 (AAQ14331) MYC1, partial (29%) 50 4e-07
TC9411 similar to UP|Q41102 (Q41102) Phaseolin G-box binding pro... 42 1e-04
TC8661 weakly similar to UP|O81348 (O81348) Symbiotic ammonium t... 42 2e-04
TC16664 similar to UP|BAD09830 (BAD09830) DNA-binding protein-li... 40 4e-04
TC18919 similar to GB|AAN73298.1|25141207|BT002301 At4g16430/dl4... 39 9e-04
TC17835 similar to UP|Q9SLC9 (Q9SLC9) At2g42300 protein, partial... 37 0.006
TC17162 similar to UP|Q8H5F1 (Q8H5F1) OJ1165_F02.16 protein, par... 35 0.022
TC16918 weakly similar to UP|Q9FJH5 (Q9FJH5) Gb|AAC98457.1 (At5g... 34 0.037
AV418628 34 0.037
CB828813 33 0.082
>CB828026
Length = 542
Score = 135 bits (340), Expect = 9e-33
Identities = 72/99 (72%), Positives = 79/99 (79%), Gaps = 3/99 (3%)
Frame = +2
Query: 120 AKEEKKDKKKGPEEPSTG---YIHVRARRGQATDSHSLAERVRREKISERMNMLQQLVPG 176
AK K+ K G + YIHVRARRGQAT+SHSLAERVRREKISERM LQ LVPG
Sbjct: 44 AKASGKNAKHGSQASDPAKEEYIHVRARRGQATNSHSLAERVRREKISERMKFLQDLVPG 223
Query: 177 CDKVTGKALVLEEIINYVQSLQHQVEFLSMKLASVNPML 215
C KVTGKA++L+EIINYVQSLQ QVEFLSMKLA+VNP L
Sbjct: 224 CSKVTGKAVMLDEIINYVQSLQRQVEFLSMKLATVNPRL 340
>AV424133
Length = 307
Score = 128 bits (322), Expect = 1e-30
Identities = 74/100 (74%), Positives = 82/100 (82%), Gaps = 3/100 (3%)
Frame = +3
Query: 165 ERMNMLQQLVPGCDKVTGKALVLEEIINYVQSLQHQVEFLSMKLASVNPMLFDMAMDLDN 224
ERM LQ+LVPGCDKVTGKAL+L+EIINYVQSLQ+QVEFLSMKLASVNPM +DMA DLD
Sbjct: 3 ERMKRLQRLVPGCDKVTGKALMLDEIINYVQSLQNQVEFLSMKLASVNPMFYDMATDLDT 182
Query: 225 LLVRPEQKVNSLASP-PLVLPCVPLCN-PNPG-TTFADTS 261
LLVRPE K+NSLASP PL CN PN TTFA+T+
Sbjct: 183 LLVRPE-KLNSLASPSPLSSVSHSHCNSPNQATTTFAETT 299
>AV766794
Length = 489
Score = 95.1 bits (235), Expect = 1e-20
Identities = 72/146 (49%), Positives = 88/146 (59%), Gaps = 19/146 (13%)
Frame = +2
Query: 1 MAAFSHHHYHPFLVDSTSRFIKMSSLEDELLQPHINTT-TLHQETSFSVT--ETSSVDQS 57
MAAFS+ HPFLVD + F+ L P + T LHQE S +VT +TS DQS
Sbjct: 71 MAAFSYQ-CHPFLVDPS--FL----LNINTGSPPLPTQFPLHQEASLNVTNQDTSCADQS 229
Query: 58 SKITISDSELSVEKNQSPETSMVVASME----------KKRRSRNGS----PLSKD--PK 101
SKITISD+E S+ KN SP++SMVV +E KRRSR S P SKD +
Sbjct: 230 SKITISDNEPSITKNLSPQSSMVVDKLEIGEQVTQKVTPKRRSRKDSSLNEPQSKDDATE 409
Query: 102 GGSKKQKKGNGGVKEEDMAKEEKKDK 127
G SKKQKK NGGVK++D A EKK++
Sbjct: 410 GRSKKQKKSNGGVKDQDKA*TEKKEQ 487
>AW428702
Length = 387
Score = 91.7 bits (226), Expect = 1e-19
Identities = 48/70 (68%), Positives = 56/70 (79%)
Frame = +3
Query: 159 RREKISERMNMLQQLVPGCDKVTGKALVLEEIINYVQSLQHQVEFLSMKLASVNPMLFDM 218
RREKISERM LQ LVPGC+K+ GKA +L+EIINYVQSLQ QVEFLSMKLA+VNP L
Sbjct: 15 RREKISERMKYLQDLVPGCNKIAGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRL--- 185
Query: 219 AMDLDNLLVR 228
++D L V+
Sbjct: 186 DFNIDELFVK 215
>BP036382
Length = 511
Score = 85.9 bits (211), Expect = 8e-18
Identities = 48/85 (56%), Positives = 65/85 (76%), Gaps = 4/85 (4%)
Frame = +3
Query: 141 VRARRGQATDSHSLAERVRREKISERMNMLQQLVPGCDKVTGKALVLEEIINYVQSLQHQ 200
VRARRGQATD HS+AER+RRE+I+ERM LQ+LVP +K T KA +L+EII+YV+ LQ Q
Sbjct: 102 VRARRGQATDPHSIAERLRRERIAERMKALQELVPNANK-TDKASMLDEIIDYVKFLQLQ 278
Query: 201 VEFLSMK----LASVNPMLFDMAMD 221
V+ LSM A+V P++ D++ +
Sbjct: 279 VKVLSMSRLGGAAAVAPLVADISSE 353
>TC9193 similar to GB|AAN28890.1|23506061|AY143951 At4g02590/T10P11_13
{Arabidopsis thaliana;}, partial (59%)
Length = 1474
Score = 75.1 bits (183), Expect = 1e-14
Identities = 39/66 (59%), Positives = 54/66 (81%)
Frame = +1
Query: 141 VRARRGQATDSHSLAERVRREKISERMNMLQQLVPGCDKVTGKALVLEEIINYVQSLQHQ 200
VRARRGQATD HS+AER+RRE+I+ER+ Q+LVP +K T +A +L+EI++YV+ L+ Q
Sbjct: 433 VRARRGQATDPHSIAERLRRERIAERIRA*QELVPSVNK-TDRAAMLDEIVDYVKFLRLQ 609
Query: 201 VEFLSM 206
V+ LSM
Sbjct: 610 VKVLSM 627
>AV772495
Length = 572
Score = 74.7 bits (182), Expect = 2e-14
Identities = 43/86 (50%), Positives = 52/86 (60%)
Frame = -3
Query: 138 YIHVRARRGQATDSHSLAERVRREKISERMNMLQQLVPGCDKVTGKALVLEEIINYVQSL 197
Y+HVR RRGQATDSHSLAER RREKI+ RM +LQ+LVPGC K+ LV E + +
Sbjct: 465 YVHVRVRRGQATDSHSLAERARREKINARMKLLQELVPGCYKL----LVSETFFY*I**I 298
Query: 198 QHQVEFLSMKLASVNPMLFDMAMDLD 223
H F+ M S+ L M D
Sbjct: 297 LHNSIFMIMP*LSIKLSLLSTIMSPD 220
>TC17246 similar to UP|Q8S490 (Q8S490) Transcription factor RAU1 (Fragment),
partial (39%)
Length = 606
Score = 61.2 bits (147), Expect = 2e-10
Identities = 33/65 (50%), Positives = 42/65 (63%)
Frame = +2
Query: 141 VRARRGQATDSHSLAERVRREKISERMNMLQQLVPGCDKVTGKALVLEEIINYVQSLQHQ 200
+RA+RG AT SLAERVRR KISERM L LVP D+ T A +L+ ++Y+Q L
Sbjct: 2 IRAQRGFATHPRSLAERVRRTKISERMRKLPDLVPNMDQPTNPADMLDLAVDYIQDLPKP 181
Query: 201 VEFLS 205
+ LS
Sbjct: 182 AQTLS 196
>TC18748 similar to GB|AAN28890.1|23506061|AY143951 At4g02590/T10P11_13
{Arabidopsis thaliana;}, partial (36%)
Length = 803
Score = 60.8 bits (146), Expect = 3e-10
Identities = 30/48 (62%), Positives = 41/48 (84%)
Frame = +1
Query: 141 VRARRGQATDSHSLAERVRREKISERMNMLQQLVPGCDKVTGKALVLE 188
VRARRGQATD HS+AER+RRE+I+ER+ LQ+LVP +K T +A++L+
Sbjct: 463 VRARRGQATDPHSIAERLRRERIAERIRALQELVPSVNK-TDRAVMLD 603
>TC20174 similar to UP|O48535 (O48535) At2g42280 protein, partial (16%)
Length = 498
Score = 52.8 bits (125), Expect = 8e-08
Identities = 29/57 (50%), Positives = 37/57 (64%)
Frame = +2
Query: 153 SLAERVRREKISERMNMLQQLVPGCDKVTGKALVLEEIINYVQSLQHQVEFLSMKLA 209
S+AERVRR +ISER+ LQ+LVP DK T A +L+ Y+ LQ Q + LS K A
Sbjct: 5 SIAERVRRTRISERIRKLQELVPNLDKQTSTAEMLDLAAVYINDLQKQFKTLSEKRA 175
>TC19009 similar to UP|AAQ14331 (AAQ14331) MYC1, partial (29%)
Length = 595
Score = 50.4 bits (119), Expect = 4e-07
Identities = 25/32 (78%), Positives = 28/32 (87%)
Frame = +2
Query: 184 ALVLEEIINYVQSLQHQVEFLSMKLASVNPML 215
ALVL+EIINY+QSLQ QVEFLSMKL +VN L
Sbjct: 2 ALVLDEIINYIQSLQRQVEFLSMKLEAVNSRL 97
>TC9411 similar to UP|Q41102 (Q41102) Phaseolin G-box binding protein PG2
(Fragment), partial (30%)
Length = 705
Score = 42.4 bits (98), Expect = 1e-04
Identities = 38/152 (25%), Positives = 72/152 (47%), Gaps = 3/152 (1%)
Frame = +2
Query: 114 VKEED---MAKEEKKDKKKGPEEPSTGYIHVRARRGQATDSHSLAERVRREKISERMNML 170
VKE D + + EK+ +K+G +P+ G + +H AER RREK+++R L
Sbjct: 95 VKEADSSRLVEPEKRPRKRG-RKPANGR--------EEPLNHVEAERQRREKLNQRFYAL 247
Query: 171 QQLVPGCDKVTGKALVLEEIINYVQSLQHQVEFLSMKLASVNPMLFDMAMDLDNLLVRPE 230
+ +VP K+ KA +L + I+Y+ L+ +++ + M +L+ +
Sbjct: 248 RAVVPNVSKM-DKASLLGDAISYITELKTKLQSSESDKTGLQKQFDAMKKELE----KTS 412
Query: 231 QKVNSLASPPLVLPCVPLCNPNPGTTFADTST 262
++ +S PP PN +F+ +S+
Sbjct: 413 EQSSSPTPPP----------PNKNKSFSSSSS 478
>TC8661 weakly similar to UP|O81348 (O81348) Symbiotic ammonium
transporter, partial (34%)
Length = 1007
Score = 41.6 bits (96), Expect = 2e-04
Identities = 23/53 (43%), Positives = 34/53 (63%)
Frame = +1
Query: 152 HSLAERVRREKISERMNMLQQLVPGCDKVTGKALVLEEIINYVQSLQHQVEFL 204
H +AER RREK+S+ + L L+PG K+ KA VL + I YV+ L+ ++ L
Sbjct: 31 HIIAERRRREKLSQSLIALAALIPGLKKM-DKASVLGDAIKYVKVLKERLRLL 186
>TC16664 similar to UP|BAD09830 (BAD09830) DNA-binding protein-like, partial
(22%)
Length = 1067
Score = 40.4 bits (93), Expect = 4e-04
Identities = 30/108 (27%), Positives = 52/108 (47%)
Frame = +1
Query: 147 QATDSHSLAERVRREKISERMNMLQQLVPGCDKVTGKALVLEEIINYVQSLQHQVEFLSM 206
+A +HS AER RR +I+ ++ L+ ++PG +K+ KA +L E+I +++ L+ S
Sbjct: 277 EALRNHSEAERRRRARINAHLDTLRTVIPGANKM-DKASLLAEVITHLKELKTNAAQASE 453
Query: 207 KLASVNPMLFDMAMDLDNLLVRPEQKVNSLASPPLVLPCVPLCNPNPG 254
L + DN +R E++ L P + C PG
Sbjct: 454 GL----------MIPKDNDEIRVEEQEGGLNGFPYSIRASLCCEYKPG 567
>TC18919 similar to GB|AAN73298.1|25141207|BT002301 At4g16430/dl4240w
{Arabidopsis thaliana;}, partial (29%)
Length = 604
Score = 39.3 bits (90), Expect = 9e-04
Identities = 25/83 (30%), Positives = 47/83 (56%)
Frame = +3
Query: 122 EEKKDKKKGPEEPSTGYIHVRARRGQATDSHSLAERVRREKISERMNMLQQLVPGCDKVT 181
+++K +K+G +P+ G + +H AER RREK+++R L+ +VP K+
Sbjct: 351 DDRKPRKRG-RKPANGR--------EEPLNHVEAERQRREKLNQRFYALRAVVPNISKM- 500
Query: 182 GKALVLEEIINYVQSLQHQVEFL 204
KA +L + I ++ LQ +++ L
Sbjct: 501 DKASLLGDAITHITDLQKKIKLL 569
>TC17835 similar to UP|Q9SLC9 (Q9SLC9) At2g42300 protein, partial (16%)
Length = 265
Score = 36.6 bits (83), Expect = 0.006
Identities = 22/60 (36%), Positives = 30/60 (49%), Gaps = 8/60 (13%)
Frame = +2
Query: 99 DPKGGSKKQKKGNGGVKE-EDMAKEEKKDKKKGPEEPSTG-------YIHVRARRGQATD 150
DP +K Q+ KE E ++ +K K + S+G Y+HVR RRGQATD
Sbjct: 71 DPAVENKNQRSAAAKRKEREKKGRDSSSNKSKSVADESSGGDGDKLPYVHVRVRRGQATD 250
>TC17162 similar to UP|Q8H5F1 (Q8H5F1) OJ1165_F02.16 protein, partial (40%)
Length = 784
Score = 34.7 bits (78), Expect = 0.022
Identities = 18/53 (33%), Positives = 32/53 (59%)
Frame = +3
Query: 153 SLAERVRREKISERMNMLQQLVPGCDKVTGKALVLEEIINYVQSLQHQVEFLS 205
++ R R + ++ +L +LVPGC KV+ L LEE +Y+ +L+ QV ++
Sbjct: 417 AIGRRKRLPAVQKKARVLGRLVPGCRKVSFPNL-LEEATDYISALEMQVRAMA 572
>TC16918 weakly similar to UP|Q9FJH5 (Q9FJH5) Gb|AAC98457.1 (At5g60800),
partial (24%)
Length = 498
Score = 33.9 bits (76), Expect = 0.037
Identities = 21/78 (26%), Positives = 36/78 (45%)
Frame = +2
Query: 56 QSSKITISDSELSVEKNQSPETSMVVASMEKKRRSRNGSPLSKDPKGGSKKQKKGNGGVK 115
+++K+T++ K + + +A KK+ +P KD G K +K K
Sbjct: 239 EANKVTVTG------KFDAVKLQAKIAEKTKKKVDLVSAPPKKDAGAGEKSPEKKPEEKK 400
Query: 116 EEDMAKEEKKDKKKGPEE 133
++ EEKK +K PEE
Sbjct: 401 SDEKKSEEKKSDEKKPEE 454
>AV418628
Length = 309
Score = 33.9 bits (76), Expect = 0.037
Identities = 26/100 (26%), Positives = 36/100 (36%), Gaps = 17/100 (17%)
Frame = +2
Query: 71 KNQSPETSMVVASMEKKRRSRNGSPLSKDPKGGSKKQKKGNG-----------------G 113
K S S +S R G S KGGSKK KK + G
Sbjct: 8 KKSSDSDSSSDSSSSGSDSDRPGVVRSGKKKGGSKKSKKSSDSDSSSDSSSSGSDSDRPG 187
Query: 114 VKEEDMAKEEKKDKKKGPEEPSTGYIHVRARRGQATDSHS 153
V K+E D G + G + A++ +++DS S
Sbjct: 188 VVRTGAKKDESSDSSSGSDSDRPGVVRTGAKKDESSDSSS 307
>CB828813
Length = 465
Score = 32.7 bits (73), Expect = 0.082
Identities = 27/115 (23%), Positives = 49/115 (42%), Gaps = 7/115 (6%)
Frame = +3
Query: 6 HHHYHPFLVDSTSR-------FIKMSSLEDELLQPHINTTTLHQETSFSVTETSSVDQSS 58
HH++HP L D T++ + L + LL+PH TTT T T++
Sbjct: 120 HHNHHPLLDDPTTKPNSSDPHQTAPNWLNNALLRPHYTTTT--------TTTTNANTNFL 275
Query: 59 KITISDSELSVEKNQSPETSMVVASMEKKRRSRNGSPLSKDPKGGSKKQKKGNGG 113
+ ++S+ + ++ S + + + G+ +S D GG K + GG
Sbjct: 276 NLQTANSDSTAAQSSS---QWLARPVLHGKVIDGGADMSHDSTGGEKTEPVTEGG 431
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.313 0.129 0.361
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,079,089
Number of Sequences: 28460
Number of extensions: 67183
Number of successful extensions: 592
Number of sequences better than 10.0: 80
Number of HSP's better than 10.0 without gapping: 577
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 583
length of query: 314
length of database: 4,897,600
effective HSP length: 90
effective length of query: 224
effective length of database: 2,336,200
effective search space: 523308800
effective search space used: 523308800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0175.2