BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0174.3
(919 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9967 214 4e-56
TC12513 similar to PIR|T00615|T00615 DNA polymerase III gamma ch... 39 0.004
AV426828 33 0.16
TC10220 similar to UP|Q84WH4 (Q84WH4) Splicing factor-like prote... 31 0.78
TC10699 similar to UP|MAD2_ARATH (Q9LU93) Mitotic spindle checkp... 29 3.8
AV412926 28 5.0
AU089540 28 5.0
TC18877 homologue to GB|AAN31107.1|23506191|AY149953 At5g61670/k... 28 5.0
TC14911 homologue to UP|Q93X55 (Q93X55) Peroxin 6 (Fragment), pa... 28 5.0
TC16397 UP|ACCD_LOTJA (Q9BBS1) Acetyl-coenzyme A carboxylase car... 28 6.6
AV409320 28 6.6
AV410248 28 8.6
AV406955 28 8.6
CN825067 28 8.6
>TC9967
Length = 612
Score = 214 bits (546), Expect = 4e-56
Identities = 104/104 (100%), Positives = 104/104 (100%)
Frame = +2
Query: 816 QNPMIKDKTLTSSDYHGMDVVTTLRSCKGNLLSSGERFLNRSFQENMGTSCSEVDSSKEK 875
QNPMIKDKTLTSSDYHGMDVVTTLRSCKGNLLSSGERFLNRSFQENMGTSCSEVDSSKEK
Sbjct: 2 QNPMIKDKTLTSSDYHGMDVVTTLRSCKGNLLSSGERFLNRSFQENMGTSCSEVDSSKEK 181
Query: 876 GNRCAHLVPSVLESDYQSNCFPQNLWLQKKFRSSYSSKSTLQGI 919
GNRCAHLVPSVLESDYQSNCFPQNLWLQKKFRSSYSSKSTLQGI
Sbjct: 182 GNRCAHLVPSVLESDYQSNCFPQNLWLQKKFRSSYSSKSTLQGI 313
>TC12513 similar to PIR|T00615|T00615 DNA polymerase III gamma chain homolog
T8K22.22 - Arabidopsis thaliana
{Arabidopsis thaliana;} , partial (9%)
Length = 708
Score = 38.9 bits (89), Expect = 0.004
Identities = 37/140 (26%), Positives = 59/140 (41%)
Frame = +3
Query: 689 GTLDAIWYKATEICQSGQLKTFLRKKGKLSSLHVDRSTSWLAIAELEFRHRHHVSKAEKS 748
G L IW E C S L+ L GKL SL + A + F +AE+
Sbjct: 111 GQLGDIWAHCIERCHSKTLRQLLHNHGKLVSL---CEVEGVLAAYVAFGDGDIKLRAERF 281
Query: 749 WKLIASSLQFILGCNIELRITYEPRASDSKYAKLKRSSFSIFSCSRRAQQKSLSSNGSES 808
+ I +S++ +L N+E+RI + P + + + S + Q++ NG+ES
Sbjct: 282 LRSITNSMEMVLRRNVEVRIIHLPDEGEIQINPPGENQAESKMASAKEQRRG-HMNGTES 458
Query: 809 DYADHVSQNPMIKDKTLTSS 828
S P + D L S+
Sbjct: 459 -----YSNLPPVLDGKLQSA 503
>AV426828
Length = 401
Score = 33.5 bits (75), Expect = 0.16
Identities = 29/113 (25%), Positives = 52/113 (45%), Gaps = 10/113 (8%)
Frame = +1
Query: 302 KFRPNSFSDLVGQNVVVRSLLGAISRGMIASFYLFHGPRGTGKTS-----ASRIFAAALN 356
K+RP S D+ +V ++ + + L+ GP GTGKTS A +++ A
Sbjct: 25 KYRPQSLDDVAAHRDIVDTIDRLTTENRLPHLLLY-GPPGTGKTSTILAVARKLYGAQYR 201
Query: 357 CLSVE----GQKPCGLCRECVL-FFSGRSKEVKEVDSVRINRTDQVKSLVKNA 404
+ +E + G+ RE + F S +S SV++ D+ ++ K+A
Sbjct: 202 NMILELNASDDRGIGVVREQIQNFASTQSLSFGVKTSVKLVLLDEADAMTKDA 360
>TC10220 similar to UP|Q84WH4 (Q84WH4) Splicing factor-like protein, partial
(31%)
Length = 917
Score = 31.2 bits (69), Expect = 0.78
Identities = 19/51 (37%), Positives = 28/51 (54%), Gaps = 2/51 (3%)
Frame = +2
Query: 192 QVRPAQVEGGIVNHVGNP--CLSVHDDFSPHSTSVDINQDFDVLDNNDNGC 240
QV PAQV +V VG P CL + + F P ST + + D D+ ++ + C
Sbjct: 356 QVLPAQVPTPVVEPVGTPSECLLLKNMFDP-STETEPDFDLDIKEDVEEEC 505
>TC10699 similar to UP|MAD2_ARATH (Q9LU93) Mitotic spindle checkpoint
protein MAD2, partial (95%)
Length = 975
Score = 28.9 bits (63), Expect = 3.8
Identities = 25/96 (26%), Positives = 39/96 (40%), Gaps = 1/96 (1%)
Frame = +2
Query: 178 IDHSRSDKKSLRANQVRPAQVEGGIVNHVGNPCLSVHDDFSPHSTSVDINQDFDVLDNND 237
+ +SDK+ +R Q +Q+ I PCL D + FDVL D
Sbjct: 362 VSREKSDKEIMREIQAIMSQIASSITYL---PCL-------------DESCVFDVLAYTD 493
Query: 238 NGCGISFCWSKS-PRFRESNLYSKIEDRPLILHQVD 272
+ F W +S P+ E+ K+ +H+VD
Sbjct: 494 TDVAVPFTWIESDPKLIENPQMVKLHSFDTKIHKVD 601
>AV412926
Length = 402
Score = 28.5 bits (62), Expect = 5.0
Identities = 25/89 (28%), Positives = 39/89 (43%), Gaps = 3/89 (3%)
Frame = -1
Query: 743 SKAEKSWKLIASSLQFILGCNIEL---RITYEPRASDSKYAKLKRSSFSIFSCSRRAQQK 799
+ A S + + + FI C I L R T S S + + R S CS +K
Sbjct: 270 NSATLSIRRMRETFCFISRCFISLQYIRPTASTYISTSSQSPIWRHDNSNSDCSESLSEK 91
Query: 800 SLSSNGSESDYADHVSQNPMIKDKTLTSS 828
L + S S + +++QNP + +KT S
Sbjct: 90 KLLFSLSTSLWTLNLTQNPCLPNKTTFKS 4
>AU089540
Length = 660
Score = 28.5 bits (62), Expect = 5.0
Identities = 14/54 (25%), Positives = 25/54 (45%), Gaps = 1/54 (1%)
Frame = +1
Query: 419 DECQLLKAETWASLSNNLENLSEHVVFVMI-TPDLDKLPRSAVSRAQTYHFPKV 471
DE Q E W + +N++N +H+ + T DL V+R Y+ ++
Sbjct: 97 DEEQSFAGEFWEDMKSNVQNHKDHIEATFLKTKDLSNRRTPGVARQYRYYVGRI 258
>TC18877 homologue to GB|AAN31107.1|23506191|AY149953 At5g61670/k11j9_190
{Arabidopsis thaliana;}, partial (42%)
Length = 530
Score = 28.5 bits (62), Expect = 5.0
Identities = 10/21 (47%), Positives = 15/21 (70%)
Frame = +2
Query: 466 YHFPKVKDADIACRLEKICVE 486
YH PK++D IA L ++CV+
Sbjct: 266 YHHPKLRDVQIALDLARLCVQ 328
>TC14911 homologue to UP|Q93X55 (Q93X55) Peroxin 6 (Fragment), partial (32%)
Length = 1418
Score = 28.5 bits (62), Expect = 5.0
Identities = 25/83 (30%), Positives = 40/83 (48%), Gaps = 13/83 (15%)
Frame = +2
Query: 293 LETPRSLSMKFRPNSFSDLVGQNVVVRSLLGAISRGMI-----------ASFYLFHGPRG 341
L TP+ ++K+ D+ G V +S+L + ++ S L +GP G
Sbjct: 191 LGTPKVPNVKWE-----DVGGLEDVKKSILDTVQLPLLHKDLFASGLRKRSGVLLYGPPG 355
Query: 342 TGKTSASRIFA--AALNCLSVEG 362
TGKT ++ A +LN LSV+G
Sbjct: 356 TGKTLLAKAVATECSLNFLSVKG 424
>TC16397 UP|ACCD_LOTJA (Q9BBS1) Acetyl-coenzyme A carboxylase carboxyl
transferase subunit beta (ACCASE beta chain) , complete
Length = 1514
Score = 28.1 bits (61), Expect = 6.6
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +3
Query: 305 PNSFSDLVGQNVVVRSLLGAISRGMIASFYLFH 337
PN++ G+ V+ ++L A+ G A+ YLFH
Sbjct: 1299 PNAYIAFAGKRVIEQTLNKAVPEGSQAAEYLFH 1397
>AV409320
Length = 392
Score = 28.1 bits (61), Expect = 6.6
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = -3
Query: 194 RPAQVEGGIVNHVGNPCLSVHDDFSPHSTSVDINQ 228
R +E G V H+G+ CL + SPH S+ ++Q
Sbjct: 114 RTVPLEFGTVGHLGHQCLK---EASPHEVSLQLDQ 19
>AV410248
Length = 432
Score = 27.7 bits (60), Expect = 8.6
Identities = 18/61 (29%), Positives = 29/61 (47%), Gaps = 1/61 (1%)
Frame = +2
Query: 4 RRHSVDIPISKTLVALRRVRSLRDPSTNSICKRSSLIDNVQWENG-SGNGISLLFPEASR 62
R+HS + PIS +++ + S R P + C + ENG +G +L + SR
Sbjct: 242 RKHSAENPISVSILHASSL*SWRSPESRGACSAIPSCLFSRGENGVEDHGHGILLRDQSR 421
Query: 63 E 63
E
Sbjct: 422 E 424
>AV406955
Length = 437
Score = 27.7 bits (60), Expect = 8.6
Identities = 28/121 (23%), Positives = 49/121 (40%), Gaps = 8/121 (6%)
Frame = +3
Query: 227 NQDFDVLDNN--------DNGCGISFCWSKSPRFRESNLYSKIEDRPLILHQVDDTDLHG 278
NQD V D +NG G+ S+S F + LYS+ + D D+
Sbjct: 33 NQDISVADMTYGRRDYYPENGRGMDDTSSESSSFLQERLYSQGQ---------DPYDMEP 185
Query: 279 DRNMRHDGGDISPTLETPRSLSMKFRPNSFSDLVGQNVVVRSLLGAISRGMIASFYLFHG 338
R+ G P L + M ++P+ + + G+ + + + A+ + + YL G
Sbjct: 186 SRHRTPSGS--LPPLNPKSATKMGYKPSGYLKVKGE--IEHAAITAVKK-QLGRHYLEDG 350
Query: 339 P 339
P
Sbjct: 351 P 353
>CN825067
Length = 687
Score = 27.7 bits (60), Expect = 8.6
Identities = 12/18 (66%), Positives = 14/18 (77%)
Frame = +1
Query: 335 LFHGPRGTGKTSASRIFA 352
LF+GP GTGKT A+R A
Sbjct: 202 LFYGPPGTGKTMAARELA 255
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.132 0.382
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,330,986
Number of Sequences: 28460
Number of extensions: 232563
Number of successful extensions: 1030
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 1025
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1029
length of query: 919
length of database: 4,897,600
effective HSP length: 99
effective length of query: 820
effective length of database: 2,080,060
effective search space: 1705649200
effective search space used: 1705649200
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0174.3