BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0174.1
(590 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV410659 255 1e-68
AV769945 143 9e-35
TC18543 similar to UP|Q94EJ1 (Q94EJ1) At1g22700/T22J18_13, parti... 42 3e-04
TC10836 similar to GB|AAL60196.1|18139887|AF441079 O-linked N-ac... 41 6e-04
TC8180 similar to UP|EXTN_TOBAC (P13983) Extensin precursor (Cel... 39 0.002
AV423919 37 0.012
BP054586 32 0.22
TC17693 similar to UP|SFR2_HUMAN (Q01130) Splicing factor, argin... 32 0.29
TC12522 UP|YCF3_LOTJA (Q9BBT4) Photosystem I assembly protein yc... 31 0.64
AV766219 30 1.4
TC17729 weakly similar to UP|Q8WQQ7 (Q8WQQ7) Asense-like protein... 28 3.2
TC19569 similar to UP|AAR11301 (AAR11301) Lectin-like receptor k... 28 3.2
TC14570 UP|CAE02645 (CAE02645) Arginine decarboxylase (Fragment... 28 4.2
BP057574 28 4.2
AV422659 28 5.5
BP039095 28 5.5
TC12245 similar to UP|Q84K11 (Q84K11) Type 5 protein serine/thre... 28 5.5
BP064290 28 5.5
TC11384 similar to UP|Q8S8Z6 (Q8S8Z6) Syringolide-induced protei... 27 9.3
TC13966 homologue to UP|O23548 (O23548) CLP proteinase homolog (... 27 9.3
>AV410659
Length = 397
Score = 255 bits (652), Expect = 1e-68
Identities = 131/131 (100%), Positives = 131/131 (100%)
Frame = +1
Query: 253 AALLLCKYASVVAGAGASASEGALADQVMAANVAKECLLAAIKADVKSAHIWGNLAYAFS 312
AALLLCKYASVVAGAGASASEGALADQVMAANVAKECLLAAIKADVKSAHIWGNLAYAFS
Sbjct: 4 AALLLCKYASVVAGAGASASEGALADQVMAANVAKECLLAAIKADVKSAHIWGNLAYAFS 183
Query: 313 ISGDHRSSSKCLEKAAKLEPNCMSTRYAVATHRMKEAERSQDPSELLSFGGNEMASIIRD 372
ISGDHRSSSKCLEKAAKLEPNCMSTRYAVATHRMKEAERSQDPSELLSFGGNEMASIIRD
Sbjct: 184 ISGDHRSSSKCLEKAAKLEPNCMSTRYAVATHRMKEAERSQDPSELLSFGGNEMASIIRD 363
Query: 373 GDSSLVELPTA 383
GDSSLVELPTA
Sbjct: 364 GDSSLVELPTA 396
>AV769945
Length = 284
Score = 143 bits (360), Expect = 9e-35
Identities = 72/72 (100%), Positives = 72/72 (100%)
Frame = -1
Query: 519 AKAMFTKSLELQPGYAPAFNNLGLVFVAEGLLEEAKYCFEKALQSDPLLDAAKSNLVKVV 578
AKAMFTKSLELQPGYAPAFNNLGLVFVAEGLLEEAKYCFEKALQSDPLLDAAKSNLVKVV
Sbjct: 284 AKAMFTKSLELQPGYAPAFNNLGLVFVAEGLLEEAKYCFEKALQSDPLLDAAKSNLVKVV 105
Query: 579 TMSKICKGLLKE 590
TMSKICKGLLKE
Sbjct: 104 TMSKICKGLLKE 69
>TC18543 similar to UP|Q94EJ1 (Q94EJ1) At1g22700/T22J18_13, partial (41%)
Length = 518
Score = 42.0 bits (97), Expect = 3e-04
Identities = 20/60 (33%), Positives = 35/60 (58%)
Frame = +1
Query: 506 LGIFYRHEKKYQRAKAMFTKSLELQPGYAPAFNNLGLVFVAEGLLEEAKYCFEKALQSDP 565
LG+ Y + K ++ A F ++++QPGY A+N+LG + ++ + A FE+ L DP
Sbjct: 118 LGVSYVRDGKLEKGIAQFETAVKIQPGYVTAWNHLGDAYESKKDYKSALKAFEEVLLFDP 297
>TC10836 similar to GB|AAL60196.1|18139887|AF441079 O-linked N-acetyl
glucosamine transferase {Arabidopsis thaliana;} ,
partial (16%)
Length = 833
Score = 40.8 bits (94), Expect = 6e-04
Identities = 22/65 (33%), Positives = 39/65 (59%)
Frame = +2
Query: 526 SLELQPGYAPAFNNLGLVFVAEGLLEEAKYCFEKALQSDPLLDAAKSNLVKVVTMSKICK 585
++E +P +A A++NL ++ +G L EA C +AL +PL+ A SNL ++ +
Sbjct: 647 AIEFRPNFADAWSNLASAYMRKGRLTEAAQCCRQALAINPLMVDAHSNLGNLMK----AQ 814
Query: 586 GLLKE 590
GL++E
Sbjct: 815 GLVQE 829
Score = 32.7 bits (73), Expect = 0.17
Identities = 18/82 (21%), Positives = 38/82 (45%)
Frame = +2
Query: 158 HELKEILSKLKESVQFDIRQAAVWNTLGFILLKTGRVQSAISVLSSLLAIAPENYDCLGN 217
H+ ++K +E+++ + A + + L + G + AI + P D N
Sbjct: 509 HDFDMCVAKNEEALRVEPHFAECYGNMANALKEKGNIDLAIRYYLIAIEFRPNFADAWSN 688
Query: 218 LGIAYLQIGNLELSAKCFQELI 239
L AY++ G L +A+C ++ +
Sbjct: 689 LASAYMRKGRLTEAAQCCRQAL 754
Score = 29.6 bits (65), Expect = 1.4
Identities = 16/50 (32%), Positives = 26/50 (52%)
Frame = +2
Query: 504 SNLGIFYRHEKKYQRAKAMFTKSLELQPGYAPAFNNLGLVFVAEGLLEEA 553
SNL Y + + A ++L + P A +NLG + A+GL++EA
Sbjct: 683 SNLASAYMRKGRLTEAAQCCRQALAINPLMVDAHSNLGNLMKAQGLVQEA 832
Score = 28.1 bits (61), Expect = 4.2
Identities = 15/50 (30%), Positives = 22/50 (44%)
Frame = +2
Query: 170 SVQFDIRQAAVWNTLGFILLKTGRVQSAISVLSSLLAIAPENYDCLGNLG 219
+++F A W+ L ++ GR+ A LAI P D NLG
Sbjct: 647 AIEFRPNFADAWSNLASAYMRKGRLTEAAQCCRQALAINPLMVDAHSNLG 796
>TC8180 similar to UP|EXTN_TOBAC (P13983) Extensin precursor (Cell wall
hydroxyproline-rich glycoprotein), partial (3%)
Length = 575
Score = 38.9 bits (89), Expect = 0.002
Identities = 23/67 (34%), Positives = 39/67 (57%)
Frame = +2
Query: 515 KYQRAKAMFTKSLELQPGYAPAFNNLGLVFVAEGLLEEAKYCFEKALQSDPLLDAAKSNL 574
K A A T+S++L P A++ LG + + + EEAK +++ALQ +P L AA+ L
Sbjct: 23 KVDPALADLTESVKLSPRNVKAWSLLGECYEGKKMEEEAKKAYQEALQLEPELSAAQEAL 202
Query: 575 VKVVTMS 581
++ + S
Sbjct: 203 KRLSSAS 223
>AV423919
Length = 422
Score = 36.6 bits (83), Expect = 0.012
Identities = 18/75 (24%), Positives = 41/75 (54%)
Frame = +2
Query: 496 KQQAHAILSNLGIFYRHEKKYQRAKAMFTKSLELQPGYAPAFNNLGLVFVAEGLLEEAKY 555
K Q +++ + LG + + A + ++L+L+P Y A+ N+G+ + +G+ +E+
Sbjct: 5 KPQDYSLWNKLGATQANSVQSADAIMAYQQALDLKPNYVRAWANMGISYANQGMYDESIR 184
Query: 556 CFEKALQSDPLLDAA 570
+ +AL +P + A
Sbjct: 185 YYVRALAMNPKAENA 229
>BP054586
Length = 340
Score = 32.3 bits (72), Expect = 0.22
Identities = 21/52 (40%), Positives = 28/52 (53%), Gaps = 2/52 (3%)
Frame = -1
Query: 187 ILLKTGRVQSAI--SVLSSLLAIAPENYDCLGNLGIAYLQIGNLELSAKCFQ 236
+LLK G I S L + L I PEN+D NLG+ G+L+ +A FQ
Sbjct: 340 LLLKLGMPSLPIARSFLMNALRIEPENHDVWFNLGLVSKSEGSLQQAADYFQ 185
>TC17693 similar to UP|SFR2_HUMAN (Q01130) Splicing factor,
arginine/serine-rich 2 (Splicing factor SC35) (SC-35)
(Splicing component, 35 kDa) (PR264 protein), partial
(8%)
Length = 751
Score = 32.0 bits (71), Expect = 0.29
Identities = 32/125 (25%), Positives = 52/125 (41%), Gaps = 5/125 (4%)
Frame = +2
Query: 471 GVSLQLSEEPSQAEKAYKQALLLATKQQAHAIL-----SNLGIFYRHEKKYQRAKAMFTK 525
G L L + +A Y+ AL +A + + SN + + KY+ TK
Sbjct: 359 GNKLFLEGKYEEALVQYELALQVAPDMPSSVEIRSIFHSNSAVCFLKLGKYENTIKECTK 538
Query: 526 SLELQPGYAPAFNNLGLVFVAEGLLEEAKYCFEKALQSDPLLDAAKSNLVKVVTMSKICK 585
+LEL P Y A G EEA +K L+ DP D A+ + ++ ++ +
Sbjct: 539 ALELNPTYVKALVRRGEAHEKLEHNEEALADLKKILEIDPSNDQARKAIRRLEPLAAEKR 718
Query: 586 GLLKE 590
+KE
Sbjct: 719 EKMKE 733
>TC12522 UP|YCF3_LOTJA (Q9BBT4) Photosystem I assembly protein ycf3, partial
(74%)
Length = 414
Score = 30.8 bits (68), Expect = 0.64
Identities = 23/99 (23%), Positives = 48/99 (48%), Gaps = 8/99 (8%)
Frame = +1
Query: 472 VSLQLSEEPSQAEKAYKQALLLATKQQAHA-ILSNLGIFYRHEKKYQRAKAMFTKSLELQ 530
+S Q ++A + Y +A+ L + IL N+G+ + ++ +A + ++LE
Sbjct: 1 MSAQSEGNYAEALQNYYEAMRLEVDPYDRSYILYNIGLIHTSNGEHTKALEYYFRALERN 180
Query: 531 PGYAPAFNNLGLV-------FVAEGLLEEAKYCFEKALQ 562
P AFNN+ ++ + +G E A+ F++A +
Sbjct: 181 PFLPQAFNNMAVICHYRGEQAIRQGDSEIAESWFDQAAE 297
>AV766219
Length = 436
Score = 29.6 bits (65), Expect = 1.4
Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = +2
Query: 481 SQAEKAYKQALLLATKQQAHA-ILSNLGIFYRHEKKYQRAKAMFTKSLELQPGYAPAFNN 539
++A + Y +A+ L + IL N+G+ + ++ +A + ++LE P AFNN
Sbjct: 8 AEALQNYYEAMRLEVDPYDRSYILYNIGLIHTSNGEHTKALEYYFRALERNPFLPQAFNN 187
Query: 540 LGLV 543
+ ++
Sbjct: 188 MAVI 199
>TC17729 weakly similar to UP|Q8WQQ7 (Q8WQQ7) Asense-like protein
(Fragment), partial (3%)
Length = 575
Score = 28.5 bits (62), Expect = 3.2
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = +2
Query: 462 GCSYTWSNLGVSLQLSEEPSQAEK 485
GC +T +LGV LQL +E +AE+
Sbjct: 425 GCGFTVLDLGVGLQLRKEEEEAEE 496
>TC19569 similar to UP|AAR11301 (AAR11301) Lectin-like receptor kinase 1;1,
partial (16%)
Length = 486
Score = 28.5 bits (62), Expect = 3.2
Identities = 14/42 (33%), Positives = 27/42 (63%)
Frame = +2
Query: 96 MHQRLNQPQKAILVYEKAEEILLRPETEIERPDLLSLVQIHH 137
+HQRL++PQ+++L+ + + LRP RP+ + ++HH
Sbjct: 170 IHQRLHRPQQSLLLLPRRKS-HLRPTPPPLRPNNQNTHRLHH 292
>TC14570 UP|CAE02645 (CAE02645) Arginine decarboxylase (Fragment) , partial
(98%)
Length = 1970
Score = 28.1 bits (61), Expect = 4.2
Identities = 12/23 (52%), Positives = 17/23 (73%)
Frame = +2
Query: 532 GYAPAFNNLGLVFVAEGLLEEAK 554
G PAF++L L ++ EGL EEA+
Sbjct: 887 GAPPAFSSLALQYLVEGLSEEAR 955
>BP057574
Length = 507
Score = 28.1 bits (61), Expect = 4.2
Identities = 21/70 (30%), Positives = 36/70 (51%), Gaps = 7/70 (10%)
Frame = -2
Query: 526 SLELQPGYAPAFNNLGLVFVAEGLLEEAKYCFEKAL---QSDPLLDAA----KSNLVKVV 578
+L ++P A+ LGL A+G + +A CF+ A +S+P+ D ++ K +
Sbjct: 500 ALRIEPTNRMAWYYLGLTHKADGRVGDAADCFQAAYMLEESEPIEDFICTH*LDSIPK*L 321
Query: 579 TMSKICKGLL 588
T+ CK LL
Sbjct: 320 TLRSDCKILL 291
>AV422659
Length = 467
Score = 27.7 bits (60), Expect = 5.5
Identities = 14/45 (31%), Positives = 24/45 (53%), Gaps = 1/45 (2%)
Frame = +1
Query: 78 HVARKMPKNAHA-HFILGLMHQRLNQPQKAILVYEKAEEILLRPE 121
H R + +HA H +L +PQ+ + + + EE+LLRP+
Sbjct: 217 HRVRHRSRGSHAEHSVLPSGADCFRRPQRFVFFHLRREELLLRPQ 351
>BP039095
Length = 472
Score = 27.7 bits (60), Expect = 5.5
Identities = 14/30 (46%), Positives = 19/30 (62%), Gaps = 4/30 (13%)
Frame = +2
Query: 133 VQIHHAQ----CLILESSSENSSDKELEPH 158
+ +HH Q CL L S+SE++ D ELE H
Sbjct: 341 LHLHHHQQGHACLFLLSNSESNLDTELEHH 430
>TC12245 similar to UP|Q84K11 (Q84K11) Type 5 protein serine/threonine
phosphatase 62 kDa isoform, partial (26%)
Length = 559
Score = 27.7 bits (60), Expect = 5.5
Identities = 26/127 (20%), Positives = 50/127 (38%), Gaps = 11/127 (8%)
Frame = +2
Query: 133 VQIHHAQCLILESSSENSSDKELEPHEL-----------KEILSKLKESVQFDIRQAAVW 181
+ H + E ++ENS+ + E +L + + E+++ D + A
Sbjct: 77 ISSQHTSKVSTEMATENSNVSKAEELKLLANDAFKARKYAQAIELYTEAIELDSQNAVYL 256
Query: 182 NTLGFILLKTGRVQSAISVLSSLLAIAPENYDCLGNLGIAYLQIGNLELSAKCFQELILK 241
F L+ SAI + + + P+ G A+L +G + + K FQ++
Sbjct: 257 ANRAFAHLRLEEYGSAILDATKAIEVDPKYAKGYYRRGAAHLGLGKFKEALKDFQQVKRM 436
Query: 242 DQNHPVA 248
N P A
Sbjct: 437 CPNDPDA 457
>BP064290
Length = 412
Score = 27.7 bits (60), Expect = 5.5
Identities = 19/55 (34%), Positives = 30/55 (54%), Gaps = 2/55 (3%)
Frame = -2
Query: 103 PQKAILVYEKAEEILLRPETEIERPDLLSLVQ--IHHAQCLILESSSENSSDKEL 155
PQK +V EE + E E+E+ ++SLVQ + Q + L+ E S+K+L
Sbjct: 366 PQKVKVVVLVVEEEQVMEEDEVEKDFVMSLVQALMLLQQQMKLKQHDEKKSEKQL 202
>TC11384 similar to UP|Q8S8Z6 (Q8S8Z6) Syringolide-induced protein 13-1-1,
partial (29%)
Length = 677
Score = 26.9 bits (58), Expect = 9.3
Identities = 10/29 (34%), Positives = 17/29 (58%)
Frame = -3
Query: 311 FSISGDHRSSSKCLEKAAKLEPNCMSTRY 339
F G H + C+E+ +K + NC+S +Y
Sbjct: 597 FQWCGPHNINQFCIERISKNQLNCLSIQY 511
>TC13966 homologue to UP|O23548 (O23548) CLP proteinase homolog (CLP
proteinase like protein), partial (20%)
Length = 417
Score = 26.9 bits (58), Expect = 9.3
Identities = 19/70 (27%), Positives = 32/70 (45%)
Frame = +3
Query: 267 AGASASEGALADQVMAANVAKECLLAAIKADVKSAHIWGNLAYAFSISGDHRSSSKCLEK 326
A A+++ AL ++A + + +A SA + LA F + SSS L
Sbjct: 36 ATAASTTFALNKPMLALPSSSRAISTPTRAIKSSASVRTTLATDFVVHSAPISSSSSLFS 215
Query: 327 AAKLEPNCMS 336
KL P+C++
Sbjct: 216 GHKLRPSCLN 245
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.315 0.129 0.364
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,502,850
Number of Sequences: 28460
Number of extensions: 123334
Number of successful extensions: 566
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 551
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 566
length of query: 590
length of database: 4,897,600
effective HSP length: 95
effective length of query: 495
effective length of database: 2,193,900
effective search space: 1085980500
effective search space used: 1085980500
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0174.1