BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0173b.7
(1526 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC13225 124 1e-28
BG662087 65 1e-10
AV410603 45 1e-04
TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 38 0.011
TC18698 37 0.018
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 37 0.024
AV420911 37 0.024
BP053311 33 0.34
BP036801 33 0.34
TC10006 similar to UP|P93396 (P93396) Transformer-SR ribonucleop... 33 0.34
BP072692 33 0.34
AV421607 32 0.58
TC10280 similar to UP|AAR20741 (AAR20741) At4g22560, partial (33%) 32 0.76
TC14241 homologue to UP|Q9XQB4 (Q9XQB4) Photosystem I reaction c... 32 0.99
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 32 0.99
TC15102 similar to UP|Q7X9B3 (Q7X9B3) 9/13 hydroperoxide lyase, ... 32 0.99
TC7812 UP|Q9LKJ6 (Q9LKJ6) Water-selective transport intrinsic me... 31 1.3
BP065763 31 1.7
TC10910 similar to UP|Q9LHN2 (Q9LHN2) Gb|AAC26693.1, partial (32%) 31 1.7
AV416351 31 1.7
>TC13225
Length = 513
Score = 124 bits (310), Expect = 1e-28
Identities = 64/172 (37%), Positives = 97/172 (56%)
Frame = +3
Query: 460 LTEDEKNQILTAVKSDEEGNPIIEDGKFIFDAVNSLIFTIAQHFVGDPSLIKDRSGDLLS 519
+T ++K+ IL K + E DAV +LI+TI HF+GDPS+ ++R+ L+
Sbjct: 6 MTPEDKSTILEKKKENGEE-----------DAVATLIYTIILHFIGDPSIFRERASSQLA 152
Query: 520 NLKCKSLGDFRWYKDTFLTRVYTREDSQHAFWKEKFLAGLPKSFGDKVREKLRSQNPGGE 579
NL C ++ D+RWYKDTF ++V RED AFWKE+F+AGLP+ KV + L N G
Sbjct: 153 NLYCPTMSDYRWYKDTFFSKVTLREDGNSAFWKERFIAGLPRLMQSKVLDNLSLFNQGNP 332
Query: 580 IPYQTLSYGQLIAIIQRVALKICQDDKIQQQLTKEKSQNRRDLGTFCEQFGI 631
+ + +LS+GQL I ++ + +SQ R +FCE +G+
Sbjct: 333 VNFGSLSFGQLHNTIVHTGIQFVLTSNFKTNAKDMQSQKRS--SSFCEXYGV 482
>BG662087
Length = 373
Score = 64.7 bits (156), Expect = 1e-10
Identities = 37/113 (32%), Positives = 63/113 (55%), Gaps = 1/113 (0%)
Frame = +1
Query: 1156 GTPRLVINYKPLNQALCWIRYPIPNKKDLLARLHDAKIFSKFDMKSGFWQIQLQEKDRYK 1215
G R+ ++Y LN+A YP+P+ L+ D ++ S D SG+ QI++ D K
Sbjct: 16 GKWRMWVDYTDLNKACPKDSYPLPSIDKLVDGASDNELLSLMDAYSGYHQIKMHPSDEDK 195
Query: 1216 TAFTVPFGQYEWNVMPFGLKNAPSEFQRIMNEIF-NP*SKFAIVYIDDVLIFS 1267
TAF Y + +PFGLKNA + +Q +M+ +F + + VY+D++++ S
Sbjct: 196 TAFMTARVNYCYQTIPFGLKNAGATYQXLMDRVFXDXVGRNMEVYLDNMIVKS 354
>AV410603
Length = 162
Score = 44.7 bits (104), Expect = 1e-04
Identities = 19/48 (39%), Positives = 31/48 (64%)
Frame = +1
Query: 1176 YPIPNKKDLLARLHDAKIFSKFDMKSGFWQIQLQEKDRYKTAFTVPFG 1223
+P+P +LL L ++ FSK D++SG+ QI ++ +DR+KT F G
Sbjct: 19 FPMPTVDELLDELRGSQFFSKLDLRSGYHQILVKPEDRHKTVFRTHHG 162
>TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (89%)
Length = 912
Score = 38.1 bits (87), Expect = 0.011
Identities = 12/22 (54%), Positives = 17/22 (76%)
Frame = +1
Query: 686 GKDVTCYNCGKPGHISRYCRLK 707
G D+ CY CG+PGH +R CR++
Sbjct: 364 GSDMKCYECGEPGHFARECRMR 429
>TC18698
Length = 808
Score = 37.4 bits (85), Expect = 0.018
Identities = 20/54 (37%), Positives = 33/54 (61%), Gaps = 1/54 (1%)
Frame = -2
Query: 1215 KTAFTVPFGQYEWNVMPFGLKNAPSEFQRIMNEIFNP*S-KFAIVYIDDVLIFS 1267
KT + Y + VMP GLKN + +QR+M++IF+ K VY++D+++ S
Sbjct: 801 KTTLKINRVNYYYQVMPLGLKNI*TTYQRLMDKIFHKQI*KNVEVYVEDMIVKS 640
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 37.0 bits (84), Expect = 0.024
Identities = 12/20 (60%), Positives = 16/20 (80%)
Frame = +1
Query: 686 GKDVTCYNCGKPGHISRYCR 705
G+D+ CY CG+PGH +R CR
Sbjct: 151 GEDLKCYECGEPGHFARECR 210
>AV420911
Length = 418
Score = 37.0 bits (84), Expect = 0.024
Identities = 12/20 (60%), Positives = 16/20 (80%)
Frame = +2
Query: 686 GKDVTCYNCGKPGHISRYCR 705
G+D+ CY CG+PGH +R CR
Sbjct: 359 GEDLKCYECGEPGHFARECR 418
>BP053311
Length = 529
Score = 33.1 bits (74), Expect = 0.34
Identities = 17/72 (23%), Positives = 27/72 (36%), Gaps = 10/72 (13%)
Frame = +2
Query: 619 RRDLGTFCEQFGIQGCP----------KKPKPRKQDPPPKQQWRRKSSQNHDHRKPKPRS 668
+ D+ +F Q G+ +K KP + P+ + K+ N H P P S
Sbjct: 29 KSDIASFASQLGLSTSQSYSGFNDVDFRKTKPNTEKATPQNTQKPKNDTNRPHEHPNPNS 208
Query: 669 KPHSTQAAKTPP 680
KP + P
Sbjct: 209 KPKXKXRPRXXP 244
>BP036801
Length = 540
Score = 33.1 bits (74), Expect = 0.34
Identities = 17/47 (36%), Positives = 27/47 (57%)
Frame = +3
Query: 636 KKPKPRKQDPPPKQQWRRKSSQNHDHRKPKPRSKPHSTQAAKTPPEN 682
++P PR+ PP ++ RR H H P P ++P + A+TPPE+
Sbjct: 345 RRPHPRRHPPPNRRLQRRLPQAPHLH--PLPTTQP--LRLARTPPES 473
>TC10006 similar to UP|P93396 (P93396) Transformer-SR ribonucleoprotein
(Fragment), partial (30%)
Length = 453
Score = 33.1 bits (74), Expect = 0.34
Identities = 22/84 (26%), Positives = 41/84 (48%), Gaps = 3/84 (3%)
Frame = +3
Query: 636 KKPKPRKQDPPPKQQWRRKSSQNHD--HRKPKPRSKPHSTQAAKTPPENRPQGKDVTCYN 693
+ P+P+ + P PK + R S++ R P+PRS+ S + + +R +G+ +
Sbjct: 165 RSPRPKSRSPRPKSRSPRAKSRSPRPRSRSPRPRSRSRSLEKQRPRSRSRSRGRSRSRSP 344
Query: 694 CGKPGHISRYCR-LKRRISELHLE 716
+ G + Y L R++E LE
Sbjct: 345 EDRNGGNTLYVTGLSSRVTERDLE 416
>BP072692
Length = 422
Score = 33.1 bits (74), Expect = 0.34
Identities = 19/51 (37%), Positives = 25/51 (48%), Gaps = 1/51 (1%)
Frame = +3
Query: 635 PKKPKPRKQDPPPKQQWRRKSSQNHDH-RKPKPRSKPHSTQAAKTPPENRP 684
P++P PR PPP+ RR+ +H H R P P PH + T P P
Sbjct: 201 PRRPPPRL--PPPRPHPRRRPLFHHPHPRLPLPPLPPHRPRWDPTSPSPPP 347
>AV421607
Length = 245
Score = 32.3 bits (72), Expect = 0.58
Identities = 18/53 (33%), Positives = 21/53 (38%)
Frame = +3
Query: 679 PPENRPQGKDVTCYNCGKPGHISRYCRLKRRISELHLEPEIEDKINNLLIQTS 731
P G CY CG+PGH SR C S + P N LL +S
Sbjct: 18 PGSGSGSGTATGCYKCGRPGHWSRDCPSSAPNSNPNPNPNTTTTPNPLLPSSS 176
>TC10280 similar to UP|AAR20741 (AAR20741) At4g22560, partial (33%)
Length = 731
Score = 32.0 bits (71), Expect = 0.76
Identities = 18/53 (33%), Positives = 27/53 (49%), Gaps = 6/53 (11%)
Frame = -1
Query: 639 KPRKQDPPPKQQWRRKSSQNHDHRK------PKPRSKPHSTQAAKTPPENRPQ 685
KPR++D P ++S H HR+ P PRS P T+ +PP + P+
Sbjct: 296 KPRRKDHHPMWDPSSETSSPHSHRQHNAHSPPNPRS-PSPTRPEPSPPHDTPR 141
>TC14241 homologue to UP|Q9XQB4 (Q9XQB4) Photosystem I reaction center
subunit III, partial (86%)
Length = 1081
Score = 31.6 bits (70), Expect = 0.99
Identities = 22/71 (30%), Positives = 32/71 (44%)
Frame = +2
Query: 627 EQFGIQGCPKKPKPRKQDPPPKQQWRRKSSQNHDHRKPKPRSKPHSTQAAKTPPENRPQG 686
E+ IQ C + +PPP S QN P P P S+ AA PP+ +
Sbjct: 65 EKKNIQTCLSQFPQTSPNPPPP------SGQN----SPPPSQNPPSSAAAAAPPQTQ--- 205
Query: 687 KDVTCYNCGKP 697
K +TC++ +P
Sbjct: 206 KTLTCHHS*RP 238
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 31.6 bits (70), Expect = 0.99
Identities = 15/52 (28%), Positives = 23/52 (43%)
Frame = +2
Query: 653 RKSSQNHDHRKPKPRSKPHSTQAAKTPPENRPQGKDVTCYNCGKPGHISRYC 704
R+ ++ D R + RS+ + E C+ CGKPGH +R C
Sbjct: 332 RERGRDRDDRGDRDRSRGYGGSRGSNGGE---------CFKCGKPGHFAREC 460
>TC15102 similar to UP|Q7X9B3 (Q7X9B3) 9/13 hydroperoxide lyase, partial
(68%)
Length = 1097
Score = 31.6 bits (70), Expect = 0.99
Identities = 34/137 (24%), Positives = 50/137 (35%)
Frame = +1
Query: 224 TNDSSVKGKGKIKIESSSSSAPPDIPKGCQLEKPLFKPFQISSRSRHSAQSLGAKNESDN 283
T S+ K S S+ PP C L P P + +S HS+ +++ S
Sbjct: 184 TTTSTTKAATNSSPPESRSTTPPSSEPTCLLA-PASPP---TPKSSHSSTPPPSRSSSTT 351
Query: 284 ELLQKVVEKLKLLNQVVPESPATTPVAVSAPAPPTAAPEPVIRRSSTRNASASSLNNIEE 343
+ + P P A +APAP P P SS ++ SSL N
Sbjct: 352 PKSKNAT------SLTAPSCPPPASPAATAPAPSKTPPNPP-TNSSKPSSCKSSLPNTT- 507
Query: 344 GESDVQSVKSIPAVNPV 360
S + S P +P+
Sbjct: 508 -PSSLSSAPPSPTTSPI 555
>TC7812 UP|Q9LKJ6 (Q9LKJ6) Water-selective transport intrinsic membrane
protein 1, complete
Length = 1149
Score = 31.2 bits (69), Expect = 1.3
Identities = 14/38 (36%), Positives = 20/38 (51%)
Frame = +1
Query: 300 VPESPATTPVAVSAPAPPTAAPEPVIRRSSTRNASASS 337
VPESP T + P PP ++P P S + + S S+
Sbjct: 190 VPESPTTNSPTTAPPPPPASSPPPSPTHSPSSSPSPSA 303
>BP065763
Length = 537
Score = 30.8 bits (68), Expect = 1.7
Identities = 16/42 (38%), Positives = 18/42 (42%)
Frame = -1
Query: 663 KPKPRSKPHSTQAAKTPPENRPQGKDVTCYNCGKPGHISRYC 704
K KP K A E R K+ CY C K GH +YC
Sbjct: 462 KAKPGRKEQKKDQALKVKEGRIH-KEHVCYFCKKAGHFKKYC 340
>TC10910 similar to UP|Q9LHN2 (Q9LHN2) Gb|AAC26693.1, partial (32%)
Length = 595
Score = 30.8 bits (68), Expect = 1.7
Identities = 16/50 (32%), Positives = 18/50 (36%), Gaps = 3/50 (6%)
Frame = -1
Query: 638 PKPRKQDPPPKQQWRRKSSQNHDHRKP---KPRSKPHSTQAAKTPPENRP 684
P P PP Q W S R P + R P T + PPE P
Sbjct: 160 PNPSPSPPPETQTWATNCSPRRTRRSPATSRRR*PPPHTTTPRPPPETSP 11
>AV416351
Length = 428
Score = 30.8 bits (68), Expect = 1.7
Identities = 16/61 (26%), Positives = 23/61 (37%), Gaps = 2/61 (3%)
Frame = +3
Query: 629 FGIQGCPKKPKPRKQDPPPKQQWRRKSSQ--NHDHRKPKPRSKPHSTQAAKTPPENRPQG 686
F P+ P+P PP K + NH P P P + + + P N P+
Sbjct: 69 FSSSSPPRNPRPPPPPPPSSSSSPEKKNSPINHVLPSPTPHQSPKTIRQSDLSPMNSPKS 248
Query: 687 K 687
K
Sbjct: 249 K 251
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.134 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,483,395
Number of Sequences: 28460
Number of extensions: 373153
Number of successful extensions: 3878
Number of sequences better than 10.0: 76
Number of HSP's better than 10.0 without gapping: 3152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3655
length of query: 1526
length of database: 4,897,600
effective HSP length: 102
effective length of query: 1424
effective length of database: 1,994,680
effective search space: 2840424320
effective search space used: 2840424320
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0173b.7