BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0172.5
(1213 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP066094 169 3e-45
AV780009 130 1e-30
BP084332 117 1e-26
BP052632 91 8e-19
BP075943 83 2e-16
BP063694 77 2e-14
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 73 3e-13
BP057233 72 5e-13
BP043850 70 2e-12
AU088980 62 5e-10
AV427921 60 2e-09
TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, p... 60 3e-09
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 58 8e-09
AV766665 58 8e-09
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 56 3e-08
AV424544 54 1e-07
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 54 2e-07
TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%) 54 2e-07
TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprot... 52 7e-07
AV766088 48 1e-05
>BP066094
Length = 532
Score = 169 bits (428), Expect(3) = 3e-45
Identities = 79/149 (53%), Positives = 110/149 (73%)
Frame = +2
Query: 791 ARLESIRLLLGVACLLKFRLYQMDVKSAFLNGYLNEEVYVEQPKGFVDPSFPDHVYRLKK 850
++ E+IRLL+ + L+QM+VKSAFLNGY++EEVYV QP G D DH+++LKK
Sbjct: 86 SKTEAIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPGXEDEKNSDHIFKLKK 265
Query: 851 ALYGLKQAPRAWYERLTEFLINNGYDKGGIDKTLFVKKNGGELMVAQIYVDDIVFGGMSN 910
+LYGLKQAPRAWYERL+ FL+ N +G +D TLF K ++++ QIYVDDI+FG +
Sbjct: 266 SLYGLKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANP 445
Query: 911 QMVEQFVEQMKSEFEMSLVGELTYFLGLQ 939
+ ++F E M++EFEM ++GEL YFLG+Q
Sbjct: 446 SLCKEFSEMMQAEFEMRMMGELKYFLGIQ 532
Score = 30.0 bits (66), Expect(3) = 3e-45
Identities = 14/18 (77%), Positives = 15/18 (82%)
Frame = +3
Query: 775 YSQIEGIDFYETFAPVAR 792
YSQ GID+ ETFAPVAR
Sbjct: 39 YSQQ*GIDYTETFAPVAR 92
Score = 21.9 bits (45), Expect(3) = 3e-45
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = +1
Query: 765 RNKSRLVAQG 774
RNK+RLVAQG
Sbjct: 10 RNKARLVAQG 39
>AV780009
Length = 529
Score = 130 bits (327), Expect = 1e-30
Identities = 67/160 (41%), Positives = 100/160 (61%), Gaps = 2/160 (1%)
Frame = -1
Query: 1035 RIIKYISGTSDYGILYSHNTNSGLTGYCDADWAGSADDRKSTSGGCFFLENNLISWFSKK 1094
R+++Y+ G G+ +S ++ L Y D+DWAG D R+S +G FL +LISW +KK
Sbjct: 517 RVLRYVKGAPAQGLFFSADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSLISWRTKK 338
Query: 1095 QNCVSLSTAEAEYIAAGSSCTQLMWMKQMLK--EYNVQQDVMTLFCDNLSAINISKNPIQ 1152
Q VS S++EAEY A ++ ++ W+ + + + NV V LFCDN SA++I+ NP
Sbjct: 337 QTTVSRSSSEAEYRALAATVCEVQWLSYLFQFLKLNVPLPV-PLFCDNQSALHIAHNPTF 161
Query: 1153 HSRTKHIDIRHHFIRELVEDGTVTLEHVSTEKQLADIFTK 1192
H RTKHI++ H +R ++ G + L +ST QLADIFTK
Sbjct: 160 HERTKHIELDCHVVRAKLQAGLIHLLPISTHHQLADIFTK 41
>BP084332
Length = 368
Score = 117 bits (293), Expect = 1e-26
Identities = 52/105 (49%), Positives = 78/105 (73%)
Frame = +2
Query: 1095 QNCVSLSTAEAEYIAAGSSCTQLMWMKQMLKEYNVQQDVMTLFCDNLSAINISKNPIQHS 1154
Q+ ++LSTAEAEYI+A TQ++WMK L++Y + + + ++CDN +AI++SKNPI HS
Sbjct: 32 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILESNIPIYCDNTAAISLSKNPILHS 211
Query: 1155 RTKHIDIRHHFIRELVEDGTVTLEHVSTEKQLADIFTKALDATQF 1199
R KHI++++HFIR+ V+ G + L+ V T+ Q ADIFTK L +F
Sbjct: 212 RAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRF 346
>BP052632
Length = 489
Score = 91.3 bits (225), Expect = 8e-19
Identities = 53/145 (36%), Positives = 83/145 (56%)
Frame = -3
Query: 544 TQYELWKGKKPTVKYFHVFGSKCYILSDREHRRKLDPKSEVGIFLGYSGNSRAYRVYNIR 603
T YELW+G++PTV YFH FG K +IL+ ++ K D K + I LGYS NS+AY V+N R
Sbjct: 484 TPYELWRGRRPTVSYFHPFGCK*FILNTNDNIGKFDNKFDKRILLGYSSNSKAYIVFNSR 305
Query: 604 TKVMMESINVVVDDTSNERTGQAHDEDDLPYECTNVEPDEPAIQFPNEQENTVSQPPLAT 663
T+V+ ESINV DD S E T + ++D L + ++ + + P N +
Sbjct: 304 TQVVEESINVKFDD*SIEATYRL-EKDFLNLDLSDQDEEVPT--------NEGQPSETSH 152
Query: 664 KEPSIRVQKIHPKENIIGDLNDGVI 688
+EPS + Q +P + + ++ ++
Sbjct: 151 EEPSTQPQTTNPWQGLTKQIHSLIV 77
>BP075943
Length = 547
Score = 83.2 bits (204), Expect = 2e-16
Identities = 46/169 (27%), Positives = 83/169 (48%), Gaps = 1/169 (0%)
Frame = -3
Query: 889 NGGELMVAQIYVDDIVFGGMSNQMVEQFVEQMKSEFEMSLVGELTYFLGLQVKQMEDTLF 948
+ G +YVDD++ G ++ + +F + +GE YFLGL++ + +
Sbjct: 542 SSGSFTALLLYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEIARSTSGIV 363
Query: 949 ITQSKYAKGIVKKFGLENAGHKRTPAATHIKLTKDEKGTDV-DPSLYRSMIGSLLYLTAS 1007
+ Q KYA ++ G + T+ + GT + D YR ++G LLYL +
Sbjct: 362 LNQRKYALQLISDSGHFGFQPRFYSHGTNSQTLGTNTGTPLTDIGSYRRIVGRLLYLNTT 183
Query: 1008 RPDITFAVGVCARYQAEPKTSHLIQVKRIIKYISGTSDYGILYSHNTNS 1056
RPDITFAV +++ + P H Q+ +KY+ G+ G+ Y ++++
Sbjct: 182 RPDITFAVNQLSQFLSAPTDIHEQQLTGFLKYL*GSPGSGLFYPASSST 36
>BP063694
Length = 511
Score = 76.6 bits (187), Expect = 2e-14
Identities = 48/168 (28%), Positives = 77/168 (45%), Gaps = 6/168 (3%)
Frame = -2
Query: 281 VFKQSGCVVKNKNKDVLMRGARSKDNCYMWTSETTFLSARCLMSKEDEVRIWHQKLGHLN 340
+F V++N+ ++ R Y+ ++ L +WH +LGH+N
Sbjct: 510 IFLDDSFVIQNRKTGAVLGRXRCDQGLYVLNQDSQALLTTSSPLPRASFELWHSRLGHVN 331
Query: 341 LKSMKRIVAEEAVRGIPKLKIQEGKVCGECQIGKQVKMSHQKLQHLTTSKVLELLHMDLM 400
+K++ + + + + + C CQ+ K ++ + S VL+L+H DL
Sbjct: 330 FDIIKQL-HKHGCLDVSSI-LPKPICCTSCQMAKSKRLVFHD-NNKRASAVLDLIHCDLR 160
Query: 401 GPMQVESLGGKRYVFVCVDDFSRFTWVEFLKEKSD------TFEVFKE 442
GP V S+ G Y + VDDFSRFTW LK KSD F+VF E
Sbjct: 159 GPSPVASIDGFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLLRFKVFME 16
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 72.8 bits (177), Expect = 3e-13
Identities = 36/91 (39%), Positives = 57/91 (62%), Gaps = 1/91 (1%)
Frame = +1
Query: 1119 WMKQMLKEYNVQQDVMTL-FCDNLSAINISKNPIQHSRTKHIDIRHHFIRELVEDGTVTL 1177
W+ +L++ V + L +CDN SA +I+ NP+ H RTKHI+I H +RE ++ G + L
Sbjct: 4 WLTYLLQDLKVPFEQPALVYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGLIHL 183
Query: 1178 EHVSTEKQLADIFTKALDATQFEKLRQLLGI 1208
+S+ + LADI+TKAL F ++ LG+
Sbjct: 184 LPISSSEPLADIYTKALSPQNFHQICAKLGL 276
>BP057233
Length = 473
Score = 72.0 bits (175), Expect = 5e-13
Identities = 34/73 (46%), Positives = 49/73 (66%)
Frame = -2
Query: 1136 LFCDNLSAINISKNPIQHSRTKHIDIRHHFIRELVEDGTVTLEHVSTEKQLADIFTKALD 1195
L+CDNLSA ++ NP+ H+R+KHI+I H+IR+ V V + +V T Q+AD TK L
Sbjct: 445 LWCDNLSAKALASNPVLHARSKHIEIDVHYIRDQVLQNEVVVAYVPTTDQIADCLTKPLS 266
Query: 1196 ATQFEKLRQLLGI 1208
T+F +LR LG+
Sbjct: 265 HTRFSQLRDKLGV 227
>BP043850
Length = 515
Score = 70.1 bits (170), Expect = 2e-12
Identities = 34/81 (41%), Positives = 51/81 (61%), Gaps = 1/81 (1%)
Frame = -1
Query: 1115 TQLMWMKQMLKEYNVQQDVMT-LFCDNLSAINISKNPIQHSRTKHIDIRHHFIRELVEDG 1173
++++W++ +L E Q T L DN SAI I+ NP+ H T+HI++ H +RE +
Sbjct: 515 SEIIWLRGLLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYDRR 336
Query: 1174 TVTLEHVSTEKQLADIFTKAL 1194
+TL HVST Q+ADI TK+L
Sbjct: 335 VITLPHVSTSVQIADILTKSL 273
>AU088980
Length = 360
Score = 62.0 bits (149), Expect = 5e-10
Identities = 33/112 (29%), Positives = 55/112 (48%)
Frame = +2
Query: 493 NGVVERKNRTLQESARAMLHAKNLPYHFWAEAINTACYIHNRVTIRQGDTVTQYELWKGK 552
N +VERK+R + RA++ LP + W A+ A Y+ N + Y+
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAYLINXLPSPLLKGQCPYQFLNND 181
Query: 553 KPTVKYFHVFGSKCYILSDREHRRKLDPKSEVGIFLGYSGNSRAYRVYNIRT 604
PT+ VFG+ CY + + +KLDP++ +FLG+ + Y V ++ T
Sbjct: 182 SPTLLDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKTGTXGYIVMDLPT 337
>AV427921
Length = 284
Score = 60.1 bits (144), Expect = 2e-09
Identities = 32/82 (39%), Positives = 45/82 (54%)
Frame = +1
Query: 485 SAPITPQQNGVVERKNRTLQESARAMLHAKNLPYHFWAEAINTACYIHNRVTIRQGDTVT 544
+A TPQQNGV ERKNRT+ R++L LP F EA+ + +I NR +
Sbjct: 10 TAAYTPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQNMM 189
Query: 545 QYELWKGKKPTVKYFHVFGSKC 566
E W G++P V +F + S+C
Sbjct: 190 PEEAWSGRQPAVDHFRI--SRC 249
>TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, partial (4%)
Length = 670
Score = 59.7 bits (143), Expect = 3e-09
Identities = 34/94 (36%), Positives = 53/94 (56%), Gaps = 1/94 (1%)
Frame = +1
Query: 1116 QLMWMKQMLKEYNVQQ-DVMTLFCDNLSAINISKNPIQHSRTKHIDIRHHFIRELVEDGT 1174
+L W+ +L++ V L+CD+ SA +I+ N + H RTKH+DI H +RE ++
Sbjct: 4 EL*WLTYILQDLRVPFISPSLLYCDSQSARHIATNAVFHERTKHLDIDCHVVREKLQAKL 183
Query: 1175 VTLEHVSTEKQLADIFTKALDATQFEKLRQLLGI 1208
L +S+ Q ADI TK L++ F L LG+
Sbjct: 184 FHLLPISSVDQTADILTKPLESGPFSHLVSKLGV 285
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 58.2 bits (139), Expect = 8e-09
Identities = 32/144 (22%), Positives = 76/144 (52%), Gaps = 9/144 (6%)
Frame = +2
Query: 869 FLINNGYDKGGIDKTLFVKK-NGGELMVAQIYVDDIVFGGMSNQMVEQFVEQMKSEFEMS 927
F+++ GY++ D + K+ + + ++ +YVDD++ G + V++ Q+ EF+M
Sbjct: 11 FIMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREFDMK 190
Query: 928 LVGELTYFLGLQV--KQMEDTLFITQSKYAKGIVKKFGLENAGHKRTPAATHIKL----- 980
+G LG+Q+ + + ++++Q Y + ++++F +++ TP + KL
Sbjct: 191 DLGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSSSMI 370
Query: 981 -TKDEKGTDVDPSLYRSMIGSLLY 1003
+ + + ++ Y S +GSL+Y
Sbjct: 371 PSSEAERMEMSRVPYASAVGSLMY 442
>AV766665
Length = 601
Score = 58.2 bits (139), Expect = 8e-09
Identities = 30/72 (41%), Positives = 41/72 (56%)
Frame = +3
Query: 1035 RIIKYISGTSDYGILYSHNTNSGLTGYCDADWAGSADDRKSTSGGCFFLENNLISWFSKK 1094
RI Y+ S G L+ S + G+ AD+ GS DR ST G FL NL++W SK+
Sbjct: 372 RIF*YLKANSRRGPLFQKEGKSSMDGFTYADYLGSIVDRLSTMGYYMFLSGNLVTWRSKQ 551
Query: 1095 QNCVSLSTAEAE 1106
QN ++ S+ EAE
Sbjct: 552 QNIIARSSGEAE 587
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 56.2 bits (134), Expect = 3e-08
Identities = 41/130 (31%), Positives = 65/130 (49%), Gaps = 6/130 (4%)
Frame = +3
Query: 1088 ISWFSKKQNCVSLSTAEAEYIAAGSSCTQLMWMKQMLKEYNVQQD------VMTLFCDNL 1141
++W S+ Q CV+LSTAEAE+IAA +C +L+WMK L+ V+T L
Sbjct: 27 VAWPSRLQKCVALSTAEAEFIAATEACHELLWMKNFLQNAWFHSHPILCCIVITKALFTL 206
Query: 1142 SAINISKNPIQHSRTKHIDIRHHFIRELVEDGTVTLEHVSTEKQLADIFTKALDATQFEK 1201
+ I + IQ T I +R+++ + LE + T+ AD+ TK+L + E
Sbjct: 207 ARILLF---IQDPSTLMFVIIG--LRDVLNSKLLELEKIHTDDDGADMMTKSLPREKLEV 371
Query: 1202 LRQLLGICLF 1211
+ G+ F
Sbjct: 372 CDMIAGMARF 401
>AV424544
Length = 276
Score = 54.3 bits (129), Expect = 1e-07
Identities = 28/90 (31%), Positives = 47/90 (52%)
Frame = +3
Query: 865 RLTEFLINNGYDKGGIDKTLFVKKNGGELMVAQIYVDDIVFGGMSNQMVEQFVEQMKSEF 924
+L+ +L GY + D +LF K V +YVDD++ G ++ ++ +F
Sbjct: 6 KLSSYLHILGYIQSAHDHSLFTKFRDASFTVILVYVDDLILAGNDLNEIQCVKNKLDIQF 185
Query: 925 EMSLVGELTYFLGLQVKQMEDTLFITQSKY 954
+ +G L YFLGL+V + LF++Q KY
Sbjct: 186 RIKDLGTLKYFLGLEVARSSCGLFLSQRKY 275
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 53.5 bits (127), Expect = 2e-07
Identities = 35/99 (35%), Positives = 51/99 (51%), Gaps = 1/99 (1%)
Frame = -1
Query: 951 QSKYAKGIVKKFGLENAGH-KRTPAATHIKLTKDEKGTDVDPSLYRSMIGSLLYLTASRP 1009
Q Y I+KKF + N+ + T I+ + G VD + Y+S+IGS+ YL R
Sbjct: 739 QKIYVDDILKKFKMTNSKYISTTIGGKEIEAGRRNGGKRVDSTYYKSLIGSVRYLNTVRS 560
Query: 1010 DITFAVGVCARYQAEPKTSHLIQVKRIIKYISGTSDYGI 1048
DI VG+ +R+ EP H +R ++YI GT GI
Sbjct: 559 DIVCGVGLRSRFM-EP*DCH*QGAQRSLRYIKGTLKDGI 446
>TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%)
Length = 550
Score = 53.5 bits (127), Expect = 2e-07
Identities = 25/80 (31%), Positives = 48/80 (59%), Gaps = 1/80 (1%)
Frame = +3
Query: 1116 QLMWMKQMLKEYNVQQDVMT-LFCDNLSAINISKNPIQHSRTKHIDIRHHFIRELVEDGT 1174
+ +W+ L + + ++ ++CDN SA++++ N + H RT++I+I H + V G
Sbjct: 21 EALWLTYALADLRIASLLLVVIYCDNRSALHLAANSVFHKRTENIEIDCHIV*VKVLFGI 200
Query: 1175 VTLEHVSTEKQLADIFTKAL 1194
+ L HV + Q+AD+FTK +
Sbjct: 201 LHLLHVPSSDQVADVFTKTI 260
>TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprotein, partial
(4%)
Length = 528
Score = 51.6 bits (122), Expect = 7e-07
Identities = 22/62 (35%), Positives = 38/62 (60%)
Frame = +3
Query: 1150 PIQHSRTKHIDIRHHFIRELVEDGTVTLEHVSTEKQLADIFTKALDATQFEKLRQLLGIC 1209
P+ T +I+ ++HF+R+ V G ++L+H T+ Q+ADI TK L +F +R +L +
Sbjct: 45 PMDVVNTLNIETKYHFLRDQVTKGKISLKHCGTDLQVADIMTKGLKTERFRNMRAMLNVV 224
Query: 1210 LF 1211
F
Sbjct: 225 SF 230
Score = 35.4 bits (80), Expect = 0.054
Identities = 13/22 (59%), Positives = 19/22 (86%)
Frame = +2
Query: 1139 DNLSAINISKNPIQHSRTKHID 1160
DN SAI+++KNP+ H R+KHI+
Sbjct: 5 DNKSAIDLAKNPVSHGRSKHIE 70
>AV766088
Length = 501
Score = 47.8 bits (112), Expect = 1e-05
Identities = 30/107 (28%), Positives = 50/107 (46%)
Frame = -3
Query: 331 IWHQKLGHLNLKSMKRIVAEEAVRGIPKLKIQEGKVCGECQIGKQVKMSHQKLQHLTTSK 390
+WH +LGH + +++ + + + I + VC C GK V++ ++T
Sbjct: 307 LWHSRLGHPSSSALRYLRSNKF---ISYELLNYSPVCESCVFGKHVRLPFVSSNNVTVMP 137
Query: 391 VLELLHMDLMGPMQVESLGGKRYVFVCVDDFSRFTWVEFLKEKSDTF 437
++LH DL + S G + YV +DDF+ F W L KS F
Sbjct: 136 -FDILHSDLWTSPVLSSAGHRFYVLF-LDDFTDFLWTFPLSNKSQVF 2
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.134 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,949,737
Number of Sequences: 28460
Number of extensions: 267903
Number of successful extensions: 1300
Number of sequences better than 10.0: 69
Number of HSP's better than 10.0 without gapping: 1288
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1294
length of query: 1213
length of database: 4,897,600
effective HSP length: 101
effective length of query: 1112
effective length of database: 2,023,140
effective search space: 2249731680
effective search space used: 2249731680
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0172.5