BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0166.9
(746 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP066990 347 4e-96
AW164028 142 2e-34
TC17621 similar to UP|BAD07830 (BAD07830) Kinesin light chain-li... 123 1e-28
BP039459 95 4e-20
BP038767 51 8e-07
TC8917 similar to UP|AAH64262 (AAH64262) MGC76273 protein, parti... 35 0.057
NP459594 Krm protein [Lotus japonicus] 32 0.28
TC8412 weakly similar to UP|Q9LW00 (Q9LW00) Genomic DNA, chromos... 32 0.28
TC19524 weakly similar to UP|SNAA_SOLTU (Q9M5P8) Alpha-soluble N... 32 0.37
TC11521 similar to UP|O23144 (O23144) Proton pump interactor (AT... 31 0.63
TC9301 similar to UP|Q7Y0Z0 (Q7Y0Z0) TPR1, complete 31 0.82
TC10836 similar to GB|AAL60196.1|18139887|AF441079 O-linked N-ac... 31 0.82
TC15102 similar to UP|Q7X9B3 (Q7X9B3) 9/13 hydroperoxide lyase, ... 30 1.1
TC9030 similar to GB|AAM63479.1|21554372|AY086477 phospholipase-... 30 1.4
TC18795 30 1.4
TC19662 homologue to GB|CAA65987.2|6015604|PSRPL9PRT ribosomal p... 30 1.8
TC13537 similar to GB|AAP21222.1|30102608|BT006414 At3g06035 {Ar... 29 3.1
TC8405 weakly similar to UP|O04083 (O04083) Lysophospholipase is... 29 3.1
TC8719 similar to UP|Q8H0I0 (Q8H0I0) Beta-1,3-glucanase-like pro... 28 4.1
BP032533 28 5.3
>BP066990
Length = 528
Score = 347 bits (890), Expect = 4e-96
Identities = 176/176 (100%), Positives = 176/176 (100%)
Frame = +1
Query: 445 GQEVEVASVDCSIGDTYLSMSRYDEAVFAYEKALTVFKTGKGENHPAVGSVFVRLADLHN 504
GQEVEVASVDCSIGDTYLSMSRYDEAVFAYEKALTVFKTGKGENHPAVGSVFVRLADLHN
Sbjct: 1 GQEVEVASVDCSIGDTYLSMSRYDEAVFAYEKALTVFKTGKGENHPAVGSVFVRLADLHN 180
Query: 505 RTGKIKESKSYCDSALRIYENPMPGVNPEEIASGLTNVSAIYESMNELEKALKLLQKALL 564
RTGKIKESKSYCDSALRIYENPMPGVNPEEIASGLTNVSAIYESMNELEKALKLLQKALL
Sbjct: 181 RTGKIKESKSYCDSALRIYENPMPGVNPEEIASGLTNVSAIYESMNELEKALKLLQKALL 360
Query: 565 IYNDSPGQQSSIAGIEAQMGVMYYMLGNYTESYNTLKNAITKLRAIGEKKSSFIGI 620
IYNDSPGQQSSIAGIEAQMGVMYYMLGNYTESYNTLKNAITKLRAIGEKKSSFIGI
Sbjct: 361 IYNDSPGQQSSIAGIEAQMGVMYYMLGNYTESYNTLKNAITKLRAIGEKKSSFIGI 528
>AW164028
Length = 313
Score = 142 bits (358), Expect = 2e-34
Identities = 66/99 (66%), Positives = 85/99 (85%)
Frame = +2
Query: 482 KTGKGENHPAVGSVFVRLADLHNRTGKIKESKSYCDSALRIYENPMPGVNPEEIASGLTN 541
K KGENHP+V SVFVRLADL++RTGK++ESKSYC++A+RIY P+PG PEEIA GLT
Sbjct: 17 KASKGENHPSVASVFVRLADLYHRTGKLRESKSYCENAMRIYSKPVPGTTPEEIAGGLTE 196
Query: 542 VSAIYESMNELEKALKLLQKALLIYNDSPGQQSSIAGIE 580
VSAI+ES++E E+ALKLLQ+A+ + + PGQQS++AGIE
Sbjct: 197 VSAIFESVDEPEEALKLLQRAMNLLEEKPGQQSTVAGIE 313
Score = 28.9 bits (63), Expect = 3.1
Identities = 15/37 (40%), Positives = 22/37 (58%)
Frame = +2
Query: 237 PERAPILLKQARDLISSGDNPQKALELALQAMNLLEK 273
PE L + + S D P++AL+L +AMNLLE+
Sbjct: 167 PEEIAGGLTEVSAIFESVDEPEEALKLLQRAMNLLEE 277
>TC17621 similar to UP|BAD07830 (BAD07830) Kinesin light chain-like protein,
partial (17%)
Length = 547
Score = 123 bits (308), Expect = 1e-28
Identities = 58/83 (69%), Positives = 71/83 (84%)
Frame = +3
Query: 654 GPYHPETLGVYSNLAGTYDAIGRLDEAIQILEYVVSVREEKLGTANPDVDDEKRRLSELL 713
GP H +TLGVYSNLA TYDA+GR+ +AI+ILEYV+ +REEKLG ANPD +DEKRRL+ELL
Sbjct: 6 GPCHQDTLGVYSNLAATYDAMGRVGDAIEILEYVLKLREEKLGIANPDFEDEKRRLAELL 185
Query: 714 KEAGRVRSRKVMSLENLLDANAR 736
KEAG+ R RK SLENL+D N++
Sbjct: 186 KEAGKTRDRKAKSLENLIDPNSK 254
>BP039459
Length = 535
Score = 95.1 bits (235), Expect = 4e-20
Identities = 54/115 (46%), Positives = 75/115 (64%), Gaps = 1/115 (0%)
Frame = +1
Query: 18 NGNHSPSKETLAPVKSPRGSLSPQRGQNGGP-NFPVDGVIEPSIEQLYENVCDMQSSDQS 76
+G+++P K+ SPR +LSP+ Q+ + +DGV++ SIEQLY NVC+M+SSDQS
Sbjct: 160 SGSYTPHKDNFNQQASPRSTLSPRSIQSSDSIDLAIDGVVDTSIEQLYYNVCEMRSSDQS 339
Query: 77 PSRKSFGSDGDESRIDSELRQLVGGRMREVEIMEEEVEVEVEKERGGSSSGEISS 131
PSR SF S G+ESRIDSEL LVGG + EI +E V E+ G ++ + S
Sbjct: 340 PSRASFYSYGEESRIDSELGHLVGGIL---EITKEVVTENKEESNGNAAEKDXVS 495
>BP038767
Length = 561
Score = 50.8 bits (120), Expect = 8e-07
Identities = 43/157 (27%), Positives = 67/157 (42%), Gaps = 23/157 (14%)
Frame = +2
Query: 147 HEIQSATTSSVSTEKSVKALNSQLDASPKSKPKG---------KSPPAKAPLERKNDKPL 197
H IQS T S NS +S +S P K+PP + + +
Sbjct: 98 HHIQSGTQFLSHPCSSYNIFNSLTSSSSQSNPLSLSSTMPGLVKTPPLRITMPQTQTPT- 274
Query: 198 LRKQTKGVASGVKSLKSSPLGKSV-------------SLNRVENSAESALDKPERAPILL 244
++++ A+ K+ SPL K + +++LD P+ P LL
Sbjct: 275 --RRSEPAATNNKTPSPSPLPKKPPSPSPSTRSSAKKKTPETPTNPDASLDNPDLGPFLL 448
Query: 245 KQARDLISSGDNPQKALELALQAMNLLE-KLGNGKPS 280
K ARD I+SG+ P KAL+ A++A E +G+PS
Sbjct: 449 KLARDTIASGEGPTKALDYAIRASKSFEICAADGEPS 559
>TC8917 similar to UP|AAH64262 (AAH64262) MGC76273 protein, partial (3%)
Length = 676
Score = 34.7 bits (78), Expect = 0.057
Identities = 26/94 (27%), Positives = 46/94 (48%), Gaps = 9/94 (9%)
Frame = +2
Query: 107 EIMEEEVEVE--------VEKERGGSSSGEISSGVGGLSSNEKKLDKVHE-IQSATTSSV 157
EI EEV+VE VEK+ + + + S L+ NE++ K E +Q+
Sbjct: 179 EIKNEEVKVEQVQESEANVEKKTEETPNEDNKSLSTLLNENEEEAKKPEEEVQAEPKEEK 358
Query: 158 STEKSVKALNSQLDASPKSKPKGKSPPAKAPLER 191
+T + VK +++ P+ +PK + P A+ E+
Sbjct: 359 ATTEEVKVETKEVNPKPEEEPKEEEPKAQVQEEK 460
>NP459594 Krm protein [Lotus japonicus]
Length = 632
Score = 32.3 bits (72), Expect = 0.28
Identities = 17/62 (27%), Positives = 32/62 (51%)
Frame = +3
Query: 337 YAMLGQLENSIMCYSSGFEVQRQVLGETDPRVGETCRYVAEANVQALQFDEAERLCQMAL 396
+ + G+L+ + + S + ++ G+ DP V C +AE +FD+AE L A+
Sbjct: 411 FFLQGKLDEAEKLFLSAVQEAKEGFGKQDPHVASACNNLAEFYRIRKEFDKAEPLYLEAI 590
Query: 397 DI 398
+I
Sbjct: 591 NI 596
>TC8412 weakly similar to UP|Q9LW00 (Q9LW00) Genomic DNA, chromosome 3, P1
clone: MSJ11, partial (39%)
Length = 1403
Score = 32.3 bits (72), Expect = 0.28
Identities = 39/150 (26%), Positives = 63/150 (42%), Gaps = 5/150 (3%)
Frame = +3
Query: 18 NGNHSPSKETLAPVKSPRGSLSPQRGQN-GGPNFPVDGVIEPSIEQLYENVCDMQSSDQS 76
+ N + A VK P G+N GPN +G + + E+L S ++
Sbjct: 570 DANQDEKRAEDADVKEPT-----HPGENTDGPND--EGKLNTADEEL------QASKEKV 710
Query: 77 PSRKSFGSDGDESRIDSELRQLVGGRMREVEIMEEEVEVEVEK----ERGGSSSGEISSG 132
+ + GSD +++ D + E+++EVE E +RG SGEI
Sbjct: 711 DDKGAEGSDAVQNKDDGSGEP---EKSEAAPTTEQKIEVEGENKEEHDRGIHESGEIKDK 881
Query: 133 VGGLSSNEKKLDKVHEIQSATTSSVSTEKS 162
G ++E+ KVH+I T S V+ S
Sbjct: 882 EGAKVNDEEHF-KVHDINQKTESGVTVNGS 968
>TC19524 weakly similar to UP|SNAA_SOLTU (Q9M5P8) Alpha-soluble NSF
attachment protein (Alpha-SNAP)
(N-ethylmaleimide-sensitive factor attachment protein,
alpha), partial (34%)
Length = 600
Score = 32.0 bits (71), Expect = 0.37
Identities = 19/56 (33%), Positives = 30/56 (52%), Gaps = 1/56 (1%)
Frame = +3
Query: 465 SRYDEAVFAYEKALTVFKTGKGENHPAVGSVFVRLADLHNRT-GKIKESKSYCDSA 519
S ++A + K+ FK GK + GSVF+R A+ H +T K + +Y D+A
Sbjct: 240 SNNEDAAELFRKSANSFKLGKSWDK--AGSVFIRAAECHMKTDSKYDAANAYVDAA 401
>TC11521 similar to UP|O23144 (O23144) Proton pump interactor
(AT4g27500/F27G19_100), partial (21%)
Length = 1005
Score = 31.2 bits (69), Expect = 0.63
Identities = 33/167 (19%), Positives = 74/167 (43%), Gaps = 6/167 (3%)
Frame = +2
Query: 53 DGVIEPSIEQLYENVCDMQSSDQSPSRKSFGSDGDESRIDSELRQLVGGRMREVEIMEEE 112
D I+ I+QL + + + + D + + S+++ L + +++E+
Sbjct: 443 DPTIKTKIDQLDKEISKINQGRFQITEALKAKRSDRAELISQIKSLRDNSRQFQSMVDEK 622
Query: 113 V-EVEVEKERGGSSSGEISSGVGGLSSNEKKLDKV-----HEIQSATTSSVSTEKSVKAL 166
+ E+E ++ G ++G GGL S+E++L+ V + IQ + S++ EK +
Sbjct: 623 IKEIEPLQQALGKLRTATNAGRGGLCSSEEELNDVIYSFQYRIQHESI-SLAEEKQILRD 799
Query: 167 NSQLDASPKSKPKGKSPPAKAPLERKNDKPLLRKQTKGVASGVKSLK 213
QL+ + + + AK + K ++ Q K + + +K
Sbjct: 800 IKQLEGTREKVVANAAMRAKLQ-DSMGQKDTIQDQVKLIGGDLDGVK 937
>TC9301 similar to UP|Q7Y0Z0 (Q7Y0Z0) TPR1, complete
Length = 1288
Score = 30.8 bits (68), Expect = 0.82
Identities = 45/211 (21%), Positives = 78/211 (36%), Gaps = 20/211 (9%)
Frame = +3
Query: 500 ADLHNRTGKIKESKSYCDSALRIYENPMPGVNPEEIASGLTNVSAIYESMNELEKALKLL 559
A G K D+A++ Y + ++ E+I S LTN +A+Y M + E +K
Sbjct: 114 AQKEKEAGNAAYKKKQFDTAIQHYSKALE-LDDEDI-SFLTNRAAVYLEMGKYEDCIKDC 287
Query: 560 QKALLIYNDSPGQQSSIAGIEAQMGVMYYMLGNYTESYNTLKNAITKLRAIGEKKSSFIG 619
KA+ + +A + G + + ++ K A+ E ++
Sbjct: 288 DKAVERGRELRADYKMVARALTRKGTALVKMAKCSADFDPAIETFQK--ALTEHRNPDTL 461
Query: 620 IALNQMGLACVQLYAFGEATELFEEAKSILEHEYG------PYHPETLG----------- 662
LN+ A L E E F+ + E E G +PE +G
Sbjct: 462 KKLNEAQKAKKDL----EQKEYFDPKLADEEREKGNEFFKQQKYPEAVGHYTESIRRNPN 629
Query: 663 ---VYSNLAGTYDAIGRLDEAIQILEYVVSV 690
YSN A Y +G + E ++ E + +
Sbjct: 630 DPRAYSNRAACYTKLGAMPEGLKDAEKCIEL 722
>TC10836 similar to GB|AAL60196.1|18139887|AF441079 O-linked N-acetyl
glucosamine transferase {Arabidopsis thaliana;} ,
partial (16%)
Length = 833
Score = 30.8 bits (68), Expect = 0.82
Identities = 20/65 (30%), Positives = 28/65 (42%)
Frame = +2
Query: 656 YHPETLGVYSNLAGTYDAIGRLDEAIQILEYVVSVREEKLGTANPDVDDEKRRLSELLKE 715
+ P +SNLA Y GRL EA Q +++ NP + D L L+K
Sbjct: 656 FRPNFADAWSNLASAYMRKGRLTEAAQCCRQALAI--------NPLMVDAHSNLGNLMKA 811
Query: 716 AGRVR 720
G V+
Sbjct: 812 QGLVQ 826
>TC15102 similar to UP|Q7X9B3 (Q7X9B3) 9/13 hydroperoxide lyase, partial
(68%)
Length = 1097
Score = 30.4 bits (67), Expect = 1.1
Identities = 23/80 (28%), Positives = 34/80 (41%)
Frame = +1
Query: 151 SATTSSVSTEKSVKALNSQLDASPKSKPKGKSPPAKAPLERKNDKPLLRKQTKGVASGVK 210
S TTS +ST S + P + P+ S + +P+ KP +S K
Sbjct: 535 SPTTSPISTPSSPENQEKPASTPPSAPPRSTSSSSSSPINTPR-KP---------SSATK 684
Query: 211 SLKSSPLGKSVSLNRVENSA 230
+ SSP G +SL+ SA
Sbjct: 685 APASSPPGSELSLHHSPPSA 744
>TC9030 similar to GB|AAM63479.1|21554372|AY086477 phospholipase-like
protein {Arabidopsis thaliana;}, partial (53%)
Length = 596
Score = 30.0 bits (66), Expect = 1.4
Identities = 30/91 (32%), Positives = 38/91 (40%), Gaps = 1/91 (1%)
Frame = +1
Query: 172 ASPKSKP-KGKSPPAKAPLERKNDKPLLRKQTKGVASGVKSLKSSPLGKSVSLNRVENSA 230
ASP S+ SPP +P K D P T+ AS S ++SP+ S+ +S
Sbjct: 247 ASPASQAGSSSSPPYTSP---KPDSPPAPSTTRDTASPTGSSRTSPMSTPSSMTASRSSI 417
Query: 231 ESALDKPERAPILLKQARDLISSGDNPQKAL 261
S P P R L SS NP AL
Sbjct: 418 PSV---PASIP------RFLPSSTPNPSAAL 483
>TC18795
Length = 755
Score = 30.0 bits (66), Expect = 1.4
Identities = 27/69 (39%), Positives = 34/69 (49%), Gaps = 9/69 (13%)
Frame = -1
Query: 120 ERGGSSSGEISSGVGGLSSNEKKLDKVHEI--------QSATTSSVSTEKS-VKALNSQL 170
E G SSG G G SSN+KKL K E +SA+ SS+S+ S K L
Sbjct: 599 EAGKKSSG----GTSGGSSNQKKLLKDVETINKVMYLDRSASRSSISSANSRSKPAGQSL 432
Query: 171 DASPKSKPK 179
+ PKS+ K
Sbjct: 431 FSDPKSRAK 405
>TC19662 homologue to GB|CAA65987.2|6015604|PSRPL9PRT ribosomal protein L9
{Pisum sativum;} , partial (75%)
Length = 507
Score = 29.6 bits (65), Expect = 1.8
Identities = 16/44 (36%), Positives = 24/44 (54%)
Frame = +2
Query: 145 KVHEIQSATTSSVSTEKSVKALNSQLDASPKSKPKGKSPPAKAP 188
K H S+ TSS+ST S+ + + A S+ + SPPA+ P
Sbjct: 140 KAHAASSSVTSSIST--SISSSSPMRTARRSSRSRPGSPPARPP 265
>TC13537 similar to GB|AAP21222.1|30102608|BT006414 At3g06035 {Arabidopsis
thaliana;} , partial (19%)
Length = 765
Score = 28.9 bits (63), Expect = 3.1
Identities = 21/73 (28%), Positives = 32/73 (43%)
Frame = -1
Query: 226 VENSAESALDKPERAPILLKQARDLISSGDNPQKALELALQAMNLLEKLGNGKPSLELVM 285
V S + +P AP+ L+ R L + + LE NL + G PSL +VM
Sbjct: 459 VRTSTIQSSSEPSPAPVYLELLRYLAYTSE*V*--LESTTVGSNLATQTGRMTPSLVVVM 286
Query: 286 CLHVTAAIYCNLG 298
+ + +CN G
Sbjct: 285 LMSHLSMCFCNFG 247
>TC8405 weakly similar to UP|O04083 (O04083) Lysophospholipase isolog,
25331-24357 (At1g11090), partial (18%)
Length = 552
Score = 28.9 bits (63), Expect = 3.1
Identities = 25/93 (26%), Positives = 35/93 (36%), Gaps = 1/93 (1%)
Frame = +3
Query: 150 QSATTSSVSTEKSVKALNSQLDAS-PKSKPKGKSPPAKAPLERKNDKPLLRKQTKGVASG 208
+SAT + ST + ++ S S PKSKP P A AP + + G
Sbjct: 114 ESATPNPTSTLPTARSSPSPSSPSTPKSKPPSS*PTATAPTPAGSSRRSASTSPPGATPS 293
Query: 209 VKSLKSSPLGKSVSLNRVENSAESALDKPERAP 241
S+ + S SA S P R+P
Sbjct: 294 SPPTSSATAAPTAS---AATSATSTASPPPRSP 383
>TC8719 similar to UP|Q8H0I0 (Q8H0I0) Beta-1,3-glucanase-like protein,
partial (48%)
Length = 643
Score = 28.5 bits (62), Expect = 4.1
Identities = 17/58 (29%), Positives = 29/58 (49%)
Frame = +2
Query: 21 HSPSKETLAPVKSPRGSLSPQRGQNGGPNFPVDGVIEPSIEQLYENVCDMQSSDQSPS 78
HSP++ + P KSP S SP ++ P P +P++ N C + S+ + P+
Sbjct: 479 HSPNRASTPPSKSPLPSRSP---RSKTPTLPPPVRSKPNLSNR*SNPC*IFSAKRGPT 643
>BP032533
Length = 441
Score = 28.1 bits (61), Expect = 5.3
Identities = 20/87 (22%), Positives = 32/87 (35%)
Frame = +3
Query: 171 DASPKSKPKGKSPPAKAPLERKNDKPLLRKQTKGVASGVKSLKSSPLGKSVSLNRVENSA 230
D S K + ++ P + PLLR + A+ SSP S S N
Sbjct: 66 DGSQNQK*RTQTTPVAVAVAIAEPNPLLRTSSNSSATSASHSSSSPFSSSPSSPSHTNHR 245
Query: 231 ESALDKPERAPILLKQARDLISSGDNP 257
L+ +P ++ + L S+ P
Sbjct: 246 THGLNLHRHSPTSSQKQKTLPSTSTPP 326
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.311 0.130 0.353
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,763,837
Number of Sequences: 28460
Number of extensions: 114591
Number of successful extensions: 575
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 565
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 574
length of query: 746
length of database: 4,897,600
effective HSP length: 97
effective length of query: 649
effective length of database: 2,136,980
effective search space: 1386900020
effective search space used: 1386900020
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0166.9