BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0166.7
(1602 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP066094 280 1e-80
BP084332 227 1e-59
AV780009 134 1e-31
BP052632 105 4e-23
BP075943 76 5e-14
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 74 1e-13
BP057233 72 9e-13
BP043850 70 3e-12
AV427921 67 2e-11
BP063694 67 2e-11
AU088980 66 5e-11
TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprot... 50 8e-11
AV766665 62 5e-10
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 59 5e-09
TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, p... 59 6e-09
TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%) 57 2e-08
AV766088 54 2e-07
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 53 3e-07
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 53 3e-07
BP033602 52 6e-07
>BP066094
Length = 532
Score = 280 bits (715), Expect(3) = 1e-80
Identities = 138/149 (92%), Positives = 142/149 (94%)
Frame = +2
Query: 1180 ARLEAIRLLISFSVNHNIILHQMDVKSAFLNGYISEEVYVHQPPGFEDEKKPDHVFKLKK 1239
++ EAIRLLISFSVNHNIILHQM+VKSAFLNGYISEEVYVHQPPG EDEK DH+FKLKK
Sbjct: 86 SKTEAIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPGXEDEKNSDHIFKLKK 265
Query: 1240 SLYGLKQAPRAWYERLSSFLLENEFVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANQ 1299
SLYGLKQAPRAWYERLSSFLLENE VRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSAN
Sbjct: 266 SLYGLKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANP 445
Query: 1300 SLCKEFSEMMQAEFEMSMMGELKYFLGIQ 1328
SLCKEFSEMMQAEFEM MMGELKYFLGIQ
Sbjct: 446 SLCKEFSEMMQAEFEMRMMGELKYFLGIQ 532
Score = 37.4 bits (85), Expect(3) = 1e-80
Identities = 17/18 (94%), Positives = 17/18 (94%)
Frame = +3
Query: 1164 YSQQEGIDYTETFAPVAR 1181
YSQQ GIDYTETFAPVAR
Sbjct: 39 YSQQ*GIDYTETFAPVAR 92
Score = 23.1 bits (48), Expect(3) = 1e-80
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +1
Query: 1154 RNKARLVAQG 1163
RNKARLVAQG
Sbjct: 10 RNKARLVAQG 39
>BP084332
Length = 368
Score = 227 bits (579), Expect(2) = 1e-59
Identities = 111/112 (99%), Positives = 111/112 (99%)
Frame = +2
Query: 1484 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQIFESNIPIYCDNTAAISLSKNPILHS 1543
QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQI ESNIPIYCDNTAAISLSKNPILHS
Sbjct: 32 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILESNIPIYCDNTAAISLSKNPILHS 211
Query: 1544 RAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFNFILKNL 1595
RAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFNFILKNL
Sbjct: 212 RAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFNFILKNL 367
Score = 21.6 bits (44), Expect(2) = 1e-59
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +1
Query: 1469 CQFLGSNL 1476
CQFLGSNL
Sbjct: 4 CQFLGSNL 27
>AV780009
Length = 529
Score = 134 bits (337), Expect = 1e-31
Identities = 68/168 (40%), Positives = 105/168 (62%), Gaps = 6/168 (3%)
Frame = -1
Query: 1421 AVKRILRYLKGTTNLGLMYKKTSEYKLSGYCDADYAGDRTERKSTSGNCQFLGSNLVSWA 1480
A R+LRY+KG GL + S KL Y D+D+AG R+S +G FLG++L+SW
Sbjct: 526 AATRVLRYVKGAPAQGLFFSADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSLISWR 347
Query: 1481 SKRQSTIALSTAEAEY--ISAAICSTQMLWMKHQLEDYQIFESN----IPIYCDNTAAIS 1534
+K+Q+T++ S++EAEY ++A +C Q W+ + +Q + N +P++CDN +A+
Sbjct: 346 TKKQTTVSRSSSEAEYRALAATVCEVQ--WLSYL---FQFLKLNVPLPVPLFCDNQSALH 182
Query: 1535 LSKNPILHSRAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKP 1582
++ NP H R KHIE+ H +R +Q G++ L + T HQ ADIFTKP
Sbjct: 181 IAHNPTFHERTKHIELDCHVVRAKLQAGLIHLLPISTHHQLADIFTKP 38
>BP052632
Length = 489
Score = 105 bits (263), Expect = 4e-23
Identities = 50/114 (43%), Positives = 71/114 (61%)
Frame = -3
Query: 925 KTPYELWKNIKPNISYFHPFGCVCYVLNTKDRLHKFDPKSSKCLLLGYSDRSKGFRFYNT 984
KTPYELW+ +P +SYFHPFGC ++LNT D + KFD K K +LLGYS SK + +N+
Sbjct: 487 KTPYELWRGRRPTVSYFHPFGCK*FILNTNDNIGKFDNKFDKRILLGYSSNSKAYIVFNS 308
Query: 985 DAKTIEESIHVRFDDKLDSDQSKLVEKFADLSINVSDKGKAPEEVEPEEDEPEE 1038
+ +EESI+V+FDD +L + F +L ++ D+ E +P E EE
Sbjct: 307 RTQVVEESINVKFDD*SIEATYRLEKDFLNLDLSDQDEEVPTNEGQPSETSHEE 146
>BP075943
Length = 547
Score = 75.9 bits (185), Expect = 5e-14
Identities = 45/159 (28%), Positives = 78/159 (48%), Gaps = 2/159 (1%)
Frame = -3
Query: 1287 IYVDDIIFGSANQSLCKEFSEMMQAEFEMSMMGELKYFLGIQVDQTPEGTYIHQSKYTKE 1346
+YVDD++ + + + +F + +GE KYFLG+++ ++ G ++Q KY +
Sbjct: 515 LYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEIARSTSGIVLNQRKYALQ 336
Query: 1347 LLKKFNMLESTVAKTPMHPTCILEKEDKSGKVCQKL--YRGMIGSLLYLTASRPDILFSV 1404
L+ + H T +G + YR ++G LLYL +RPDI F+V
Sbjct: 335 LISDSGHF-GFQPRFYSHGTNSQTLGTNTGTPLTDIGSYRRIVGRLLYLNTTRPDITFAV 159
Query: 1405 HLCARFQSDPRETHLTAVKRILRYLKGTTNLGLMYKKTS 1443
+ ++F S P + H + L+YL G+ GL Y +S
Sbjct: 158 NQLSQFLSAPTDIHEQQLTGFLKYL*GSPGSGLFYPASS 42
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 74.3 bits (181), Expect = 1e-13
Identities = 33/91 (36%), Positives = 55/91 (60%), Gaps = 1/91 (1%)
Frame = +1
Query: 1508 WMKHQLEDYQI-FESNIPIYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYVQKGVLLL 1566
W+ + L+D ++ FE +YCDN +A ++ NP+ H R KHIE+ H +R+ +QKG++ L
Sbjct: 4 WLTYLLQDLKVPFEQPALVYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGLIHL 183
Query: 1567 KFVDTDHQWADIFTKPLAEDRFNFILKNLNM 1597
+ + ADI+TK L+ F+ I L +
Sbjct: 184 LPISSSEPLADIYTKALSPQNFHQICAKLGL 276
>BP057233
Length = 473
Score = 71.6 bits (174), Expect = 9e-13
Identities = 32/77 (41%), Positives = 51/77 (65%)
Frame = -2
Query: 1525 IYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLA 1584
++CDN +A +L+ NP+LH+R+KHIE+ H+IRD V + +++ +V T Q AD TKPL+
Sbjct: 445 LWCDNLSAKALASNPVLHARSKHIEIDVHYIRDQVLQNEVVVAYVPTTDQIADCLTKPLS 266
Query: 1585 EDRFNFILKNLNMDFCP 1601
RF+ + L + P
Sbjct: 265 HTRFSQLRDKLGVIHSP 215
>BP043850
Length = 515
Score = 70.1 bits (170), Expect = 3e-12
Identities = 33/93 (35%), Positives = 54/93 (57%), Gaps = 1/93 (1%)
Frame = -1
Query: 1504 TQMLWMKHQLEDYQIFESN-IPIYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYVQKG 1562
++++W++ L + +S P++ DNT+AI ++ NP+ H +HIEV H +R+ +
Sbjct: 515 SEIIWLRGLLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYDRR 336
Query: 1563 VLLLKFVDTDHQWADIFTKPLAEDRFNFILKNL 1595
V+ L V T Q ADI TK L R NF++ L
Sbjct: 335 VITLPHVSTSVQIADILTKSLTRQRHNFLVSKL 237
>AV427921
Length = 284
Score = 67.0 bits (162), Expect = 2e-11
Identities = 33/79 (41%), Positives = 45/79 (56%)
Frame = +1
Query: 871 TPQQNGVVERKNRTLQEMARTMLQETGMAKHFWAEAVNTACYIQNRISVRPILNKTPYEL 930
TPQQNGV ERKNRT+ M R++L +G+ K F EAV + +I NR + N P E
Sbjct: 22 TPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQNMMPEEA 201
Query: 931 WKNIKPNISYFHPFGCVCY 949
W +P + +F C+ Y
Sbjct: 202 WSGRQPAVDHFRISRCLAY 258
>BP063694
Length = 511
Score = 67.0 bits (162), Expect = 2e-11
Identities = 44/122 (36%), Positives = 60/122 (49%), Gaps = 3/122 (2%)
Frame = -2
Query: 713 VWHRRLGHASMRKISQLSK---LNLVRGLPNLKFASDALCETCQKGKFTKVPFKAKNVVS 769
+WH RLGH + I QL K L++ LP C +CQ K ++ F N
Sbjct: 360 LWHSRLGHVNFDIIKQLHKHGCLDVSSILPK-----PICCTSCQMAKSKRLVFHDNNK-R 199
Query: 770 TSRPLELLHIDLFGPVKTESIGGKRYGMVIVDDYSRWTWVKFLTRKDESHAVFSTFIAQV 829
S L+L+H DL GP SI G Y ++ VDD+SR+TW L RK + V F +
Sbjct: 198 ASAVLDLIHCDLRGPSPVASIDGFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLLRFKVFM 19
Query: 830 QN 831
+N
Sbjct: 18 EN 13
>AU088980
Length = 360
Score = 65.9 bits (159), Expect = 5e-11
Identities = 30/105 (28%), Positives = 52/105 (48%)
Frame = +2
Query: 875 NGVVERKNRTLQEMARTMLQETGMAKHFWAEAVNTACYIQNRISVRPILNKTPYELWKNI 934
N +VERK+R + + R ++ + + K+ W AV A Y+ N + + + PY+ N
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAYLINXLPSPLLKGQCPYQFLNND 181
Query: 935 KPNISYFHPFGCVCYVLNTKDRLHKFDPKSSKCLLLGYSDRSKGF 979
P + FG +CY K DP++ KC+ LG+ + G+
Sbjct: 182 SPTLLDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKTGTXGY 316
>TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprotein, partial
(4%)
Length = 528
Score = 49.7 bits (117), Expect(2) = 8e-11
Identities = 26/51 (50%), Positives = 32/51 (61%)
Frame = +3
Query: 1547 HIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFNFILKNLNM 1597
+IE KYHF+RD V KG + LK TD Q ADI TK L +RF + LN+
Sbjct: 69 NIETKYHFLRDQVTKGKISLKHCGTDLQVADIMTKGLKTERFRNMRAMLNV 221
Score = 35.4 bits (80), Expect(2) = 8e-11
Identities = 14/22 (63%), Positives = 18/22 (81%)
Frame = +2
Query: 1528 DNTAAISLSKNPILHSRAKHIE 1549
DN +AI L+KNP+ H R+KHIE
Sbjct: 5 DNKSAIDLAKNPVSHGRSKHIE 70
>AV766665
Length = 601
Score = 62.4 bits (150), Expect = 5e-10
Identities = 32/76 (42%), Positives = 44/76 (57%)
Frame = +3
Query: 1420 TAVKRILRYLKGTTNLGLMYKKTSEYKLSGYCDADYAGDRTERKSTSGNCQFLGSNLVSW 1479
T RI YLK + G +++K + + G+ ADY G +R ST G FL NLV+W
Sbjct: 360 TFTTRIF*YLKANSRRGPLFQKEGKSSMDGFTYADYLGSIVDRLSTMGYYMFLSGNLVTW 539
Query: 1480 ASKRQSTIALSTAEAE 1495
SK+Q+ IA S+ EAE
Sbjct: 540 RSKQQNIIARSSGEAE 587
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 59.3 bits (142), Expect = 5e-09
Identities = 37/145 (25%), Positives = 71/145 (48%), Gaps = 9/145 (6%)
Frame = +2
Query: 1257 SFLLENEFVRGKVDTTLFCKTYKD-DILIVQIYVDDIIFGSANQSLCKEFSEMMQAEFEM 1315
SF++ + R D + K + D D +I+ +YVDD++ N+ +E + EF+M
Sbjct: 8 SFIMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREFDM 187
Query: 1316 SMMGELKYFLGIQV--DQTPEGTYIHQSKYTKELLKKFNMLESTVAKTP------MHPTC 1367
+G LG+Q+ D+ ++ Q Y +++L++FNM + TP + +
Sbjct: 188 KDLGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSSSM 367
Query: 1368 ILEKEDKSGKVCQKLYRGMIGSLLY 1392
I E + ++ + Y +GSL+Y
Sbjct: 368 IPSSEAERMEMSRVPYASAVGSLMY 442
>TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, partial (4%)
Length = 670
Score = 58.9 bits (141), Expect = 6e-09
Identities = 28/91 (30%), Positives = 52/91 (56%), Gaps = 1/91 (1%)
Frame = +1
Query: 1508 WMKHQLEDYQI-FESNIPIYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYVQKGVLLL 1566
W+ + L+D ++ F S +YCD+ +A ++ N + H R KH+++ H +R+ +Q + L
Sbjct: 13 WLTYILQDLRVPFISPSLLYCDSQSARHIATNAVFHERTKHLDIDCHVVREKLQAKLFHL 192
Query: 1567 KFVDTDHQWADIFTKPLAEDRFNFILKNLNM 1597
+ + Q ADI TKPL F+ ++ L +
Sbjct: 193 LPISSVDQTADILTKPLESGPFSHLVSKLGV 285
>TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%)
Length = 550
Score = 57.0 bits (136), Expect = 2e-08
Identities = 29/82 (35%), Positives = 48/82 (58%), Gaps = 1/82 (1%)
Frame = +3
Query: 1505 QMLWMKHQLEDYQIFESN-IPIYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYVQKGV 1563
+ LW+ + L D +I + IYCDN +A+ L+ N + H R ++IE+ H + V G+
Sbjct: 21 EALWLTYALADLRIASLLLVVIYCDNRSALHLAANSVFHKRTENIEIDCHIV*VKVLFGI 200
Query: 1564 LLLKFVDTDHQWADIFTKPLAE 1585
L L V + Q AD+FTK +++
Sbjct: 201 LHLLHVPSSDQVADVFTKTISQ 266
>AV766088
Length = 501
Score = 53.9 bits (128), Expect = 2e-07
Identities = 36/108 (33%), Positives = 58/108 (53%), Gaps = 1/108 (0%)
Frame = -3
Query: 711 QWVWHRRLGHASMRKISQLSKLNLVRGLPNLKFASDALCETCQKGKFTKVPFKAKNVVST 770
Q +WH RLGH S + L + N L ++ +CE+C GK ++PF + N V T
Sbjct: 313 QSLWHSRLGHPSSSALRYL-RSNKFISYELLNYSP--VCESCVFGKHVRLPFVSSNNV-T 146
Query: 771 SRPLELLHIDLF-GPVKTESIGGKRYGMVIVDDYSRWTWVKFLTRKDE 817
P ++LH DL+ PV S G R+ ++ +DD++ + W L+ K +
Sbjct: 145 VMPFDILHSDLWTSPVL--SSAGHRFYVLFLDDFTDFLWTFPLSNKSQ 8
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 53.1 bits (126), Expect = 3e-07
Identities = 37/114 (32%), Positives = 61/114 (53%), Gaps = 3/114 (2%)
Frame = +3
Query: 1477 VSWASKRQSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQIFESNIPIYC---DNTAAI 1533
V+W S+ Q +ALSTAEAE+I+A ++LWMK+ L++ F S+ PI C A
Sbjct: 27 VAWPSRLQKCVALSTAEAEFIAATEACHELLWMKNFLQN-AWFHSH-PILCCIVITKALF 200
Query: 1534 SLSKNPILHSRAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDR 1587
+L++ + + +RD + +L L+ + TD AD+ TK L ++
Sbjct: 201 TLARILLFIQDPSTLMFVIIGLRDVLNSKLLELEKIHTDDDGADMMTKSLPREK 362
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 53.1 bits (126), Expect = 3e-07
Identities = 36/99 (36%), Positives = 49/99 (49%), Gaps = 1/99 (1%)
Frame = -1
Query: 1340 QSKYTKELLKKFNMLESTVAKTPMHPTCILEKEDKSGK-VCQKLYRGMIGSLLYLTASRP 1398
Q Y ++LKKF M S T + I GK V Y+ +IGS+ YL R
Sbjct: 739 QKIYVDDILKKFKMTNSKYISTTIGGKEIEAGRRNGGKRVDSTYYKSLIGSVRYLNTVRS 560
Query: 1399 DILFSVHLCARFQSDPRETHLTAVKRILRYLKGTTNLGL 1437
DI+ V L +RF +P + H +R LRY+KGT G+
Sbjct: 559 DIVCGVGLRSRFM-EP*DCH*QGAQRSLRYIKGTLKDGI 446
>BP033602
Length = 533
Score = 52.4 bits (124), Expect = 6e-07
Identities = 30/99 (30%), Positives = 53/99 (53%)
Frame = +3
Query: 706 SVNEEQWVWHRRLGHASMRKISQLSKLNLVRGLPNLKFASDALCETCQKGKFTKVPFKAK 765
SV ++ +WH RLGH + + + L +L + + S CE+C K + P+ ++
Sbjct: 255 SVRDQIMLWHNRLGHPNFQYLRHLFP-DLFKNVN----CSSLECESCVLAKNQRAPYYSQ 419
Query: 766 NVVSTSRPLELLHIDLFGPVKTESIGGKRYGMVIVDDYS 804
SRP L+H D++GP K + GKR+ + +DD++
Sbjct: 420 PY-HASRPFYLIHSDVWGPSKITTQFGKRWFVTFIDDHT 533
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.332 0.142 0.437
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,592,699
Number of Sequences: 28460
Number of extensions: 365753
Number of successful extensions: 2228
Number of sequences better than 10.0: 69
Number of HSP's better than 10.0 without gapping: 2187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2217
length of query: 1602
length of database: 4,897,600
effective HSP length: 103
effective length of query: 1499
effective length of database: 1,966,220
effective search space: 2947363780
effective search space used: 2947363780
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (22.0 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0166.7