BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0166.5
(1017 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP066094 261 8e-75
BP084332 188 4e-48
AV780009 126 2e-29
BP052632 96 3e-20
BP057233 75 5e-14
BP075943 74 9e-14
BP043850 69 3e-12
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 69 5e-12
BP085942 68 6e-12
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 67 2e-11
AV766665 67 2e-11
AU088980 66 2e-11
TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprot... 50 3e-11
BP063694 62 5e-10
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 58 9e-09
TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%) 57 1e-08
AV427921 54 1e-07
TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, p... 53 3e-07
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 50 1e-06
TC19474 weakly similar to UP|Q9FJA1 (Q9FJA1) Similarity to retro... 38 7e-06
>BP066094
Length = 532
Score = 261 bits (666), Expect(3) = 8e-75
Identities = 128/149 (85%), Positives = 138/149 (91%)
Frame = +2
Query: 591 ARLEAIRLLISFSVNHNITLHQMDVKSAFLNGYISEEVYVKQPPGFEDDKYPDHVYKLKK 650
++ EAIRLLISFSVNHNI LHQM+VKSAFLNGYISEEVYV QPPG ED+K DH++KLKK
Sbjct: 86 SKTEAIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPGXEDEKNSDHIFKLKK 265
Query: 651 SLYGLKQAPRAWYERLSSFLLQNEFVRGKGDNTLFCITYKNDILIVQIYVDDIIFGSANP 710
SLYGLKQAPRAWYERLSSFLL+NE VRGK D TLFC TYK+DILIVQIYVDDIIFGSANP
Sbjct: 266 SLYGLKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANP 445
Query: 711 SLCKEFSKLMQAEFEMSMMGELKYFLGIQ 739
SLCKEFS++MQAEFEM MMGELKYFLGIQ
Sbjct: 446 SLCKEFSEMMQAEFEMRMMGELKYFLGIQ 532
Score = 36.2 bits (82), Expect(3) = 8e-75
Identities = 16/18 (88%), Positives = 17/18 (93%)
Frame = +3
Query: 575 YSQQEGIDYTETFSPVAR 592
YSQQ GIDYTETF+PVAR
Sbjct: 39 YSQQ*GIDYTETFAPVAR 92
Score = 23.1 bits (48), Expect(3) = 8e-75
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +1
Query: 565 RNKARLVAQG 574
RNKARLVAQG
Sbjct: 10 RNKARLVAQG 39
>BP084332
Length = 368
Score = 188 bits (477), Expect = 4e-48
Identities = 89/112 (79%), Positives = 100/112 (88%)
Frame = +2
Query: 895 QNTIALSTAEAEYVSAATCCTQTIWMKNHLEDYGLSLKKVPIYCDNTAAISLSKNPILHS 954
Q+TIALSTAEAEY+SAA C TQ +WMK+ LEDY + +PIYCDNTAAISLSKNPILHS
Sbjct: 32 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILESNIPIYCDNTAAISLSKNPILHS 211
Query: 955 RAKHIEVKYHYIRDHVQKGTLSLEYVDTDHQWADIFTKPLAEDRFLFILENL 1006
RAKHIEVKYH+IRD+VQKG L L++VDTDHQWADIFTKPLAEDRF FIL+NL
Sbjct: 212 RAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFNFILKNL 367
>AV780009
Length = 529
Score = 126 bits (316), Expect = 2e-29
Identities = 62/163 (38%), Positives = 98/163 (60%), Gaps = 1/163 (0%)
Frame = -1
Query: 832 AVKRILKYLKGTTNLGLMYRKTSEYTLSGFCDADFAGDRVERKSTSGSCHFLGSNLVTWS 891
A R+L+Y+KG GL + S L + D+D+AG R+S +G FLG++L++W
Sbjct: 526 AATRVLRYVKGAPAQGLFFSADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSLISWR 347
Query: 892 SKRQNTIALSTAEAEYVSAATCCTQTIWMKNHLEDYGLSLK-KVPIYCDNTAAISLSKNP 950
+K+Q T++ S++EAEY + A + W+ + L++ VP++CDN +A+ ++ NP
Sbjct: 346 TKKQTTVSRSSSEAEYRALAATVCEVQWLSYLFQFLKLNVPLPVPLFCDNQSALHIAHNP 167
Query: 951 ILHSRAKHIEVKYHYIRDHVQKGTLSLEYVDTDHQWADIFTKP 993
H R KHIE+ H +R +Q G + L + T HQ ADIFTKP
Sbjct: 166 TFHERTKHIELDCHVVRAKLQAGLIHLLPISTHHQLADIFTKP 38
>BP052632
Length = 489
Score = 95.9 bits (237), Expect = 3e-20
Identities = 49/126 (38%), Positives = 70/126 (54%)
Frame = -3
Query: 334 KTPYELCKGRQPDISYFHPFGSTCYMLNTKEQLGKFDSKALKCYFLGYSERSKGFRIYNI 393
KTPYEL +GR+P +SYFHPFG ++LNT + +GKFD+K K LGYS SK + ++N
Sbjct: 487 KTPYELWRGRRPTVSYFHPFGCK*FILNTNDNIGKFDNKFDKRILLGYSSNSKAYIVFNS 308
Query: 394 IHQTVEESIQIRFDGKLGSEKSKLFERFADLSIDCSEANQPKNSSEDVAPEAEASEAAPT 453
Q VEESI ++FD +L + F +L + + P N + E P
Sbjct: 307 RTQVVEESINVKFDD*SIEATYRLEKDFLNLDLSDQDEEVPTNEGQPSETSHEEPSTQPQ 128
Query: 454 TSDQLQ 459
T++ Q
Sbjct: 127 TTNPWQ 110
>BP057233
Length = 473
Score = 75.1 bits (183), Expect = 5e-14
Identities = 35/81 (43%), Positives = 52/81 (63%)
Frame = -2
Query: 932 KKVPIYCDNTAAISLSKNPILHSRAKHIEVKYHYIRDHVQKGTLSLEYVDTDHQWADIFT 991
+K ++CDN +A +L+ NP+LH+R+KHIE+ HYIRD V + + + YV T Q AD T
Sbjct: 457 RKPILWCDNLSAKALASNPVLHARSKHIEIDVHYIRDQVLQNEVVVAYVPTTDQIADCLT 278
Query: 992 KPLAEDRFLFILENLNMDFCP 1012
KPL+ RF + + L + P
Sbjct: 277 KPLSHTRFSQLRDKLGVIHSP 215
>BP075943
Length = 547
Score = 74.3 bits (181), Expect = 9e-14
Identities = 46/162 (28%), Positives = 79/162 (48%), Gaps = 5/162 (3%)
Frame = -3
Query: 698 IYVDDIIFGSANPSLCKEFSKLMQAEFEMSMMGELKYFLGIQIDQRPGVTYIHQKKYTLE 757
+YVDD++ + + + +F + +GE KYFLG++I + ++Q+KY L+
Sbjct: 515 LYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEIARSTSGIVLNQRKYALQ 336
Query: 758 LLKKFNMSDCNISKTPMHPTCILEKEEVSSKVCQKL-----YRGMIGSLLYLTASRPDIL 812
L+ + P + + + + L YR ++G LLYL +RPDI
Sbjct: 335 LISDSG----HFGFQPRFYSHGTNSQTLGTNTGTPLTDIGSYRRIVGRLLYLNTTRPDIT 168
Query: 813 FSVHLCARFQSDPRETHLTAVKRILKYLKGTTNLGLMYRKTS 854
F+V+ ++F S P + H + LKYL G+ GL Y +S
Sbjct: 167 FAVNQLSQFLSAPTDIHEQQLTGFLKYL*GSPGSGLFYPASS 42
>BP043850
Length = 515
Score = 69.3 bits (168), Expect = 3e-12
Identities = 33/93 (35%), Positives = 54/93 (57%), Gaps = 1/93 (1%)
Frame = -1
Query: 915 TQTIWMKNHLEDYG-LSLKKVPIYCDNTAAISLSKNPILHSRAKHIEVKYHYIRDHVQKG 973
++ IW++ L + G L + P++ DNT+AI ++ NP+ H +HIEV H +R+ +
Sbjct: 515 SEIIWLRGLLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYDRR 336
Query: 974 TLSLEYVDTDHQWADIFTKPLAEDRFLFILENL 1006
++L +V T Q ADI TK L R F++ L
Sbjct: 335 VITLPHVSTSVQIADILTKSLTRQRHNFLVSKL 237
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 68.6 bits (166), Expect = 5e-12
Identities = 43/146 (29%), Positives = 71/146 (48%), Gaps = 15/146 (10%)
Frame = +2
Query: 673 NEFVRGKGDNTL---FCITYK----NDILIVQIYVDDIIFGSANPSLCKEFSKLMQAEFE 725
+ F+ G N L C +K ND +I+ +YVDD++ N +E + EF+
Sbjct: 5 DSFIMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREFD 184
Query: 726 MSMMGELKYFLGIQI--DQRPGVTYIHQKKYTLELLKKFNMSDCNISKTPMHPTCILEKE 783
M +G LG+QI D++ ++ QK Y ++L++FNM DCN TP+ L
Sbjct: 185 MKDLGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSSS 364
Query: 784 EVSSKVCQKL------YRGMIGSLLY 803
+ S +++ Y +GSL+Y
Sbjct: 365 MIPSSEAERMEMSRVPYASAVGSLMY 442
>BP085942
Length = 208
Score = 68.2 bits (165), Expect = 6e-12
Identities = 35/60 (58%), Positives = 43/60 (71%)
Frame = +3
Query: 203 KYGLVIVDDYSRWTWVKFLRHKDETHTIFTNFITQVQKEFQTSVITVRSDHGGEFENKAF 262
K+ +V++D ++ HKDETHT FTNFITQVQKEFQTSVITVRSDHG ++F
Sbjct: 9 KHRVVLLDQ-------NWILHKDETHTRFTNFITQVQKEFQTSVITVRSDHGWRI*EQSF 167
Score = 34.7 bits (78), Expect = 0.078
Identities = 15/17 (88%), Positives = 15/17 (88%)
Frame = +1
Query: 254 GGEFENKAFEELFNSQG 270
GGEFENKA EE FNSQG
Sbjct: 142 GGEFENKALEEWFNSQG 192
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 66.6 bits (161), Expect = 2e-11
Identities = 31/91 (34%), Positives = 51/91 (55%), Gaps = 1/91 (1%)
Frame = +1
Query: 919 WMKNHLEDYGLSLKKVP-IYCDNTAAISLSKNPILHSRAKHIEVKYHYIRDHVQKGTLSL 977
W+ L+D + ++ +YCDN +A ++ NP+ H R KHIE+ H +R+ +QKG + L
Sbjct: 4 WLTYLLQDLKVPFEQPALVYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGLIHL 183
Query: 978 EYVDTDHQWADIFTKPLAEDRFLFILENLNM 1008
+ + ADI+TK L+ F I L +
Sbjct: 184 LPISSSEPLADIYTKALSPQNFHQICAKLGL 276
>AV766665
Length = 601
Score = 66.6 bits (161), Expect = 2e-11
Identities = 35/76 (46%), Positives = 46/76 (60%)
Frame = +3
Query: 831 TAVKRILKYLKGTTNLGLMYRKTSEYTLSGFCDADFAGDRVERKSTSGSCHFLGSNLVTW 890
T RI YLK + G +++K + ++ GF AD+ G V+R ST G FL NLVTW
Sbjct: 360 TFTTRIF*YLKANSRRGPLFQKEGKSSMDGFTYADYLGSIVDRLSTMGYYMFLSGNLVTW 539
Query: 891 SSKRQNTIALSTAEAE 906
SK+QN IA S+ EAE
Sbjct: 540 RSKQQNIIARSSGEAE 587
>AU088980
Length = 360
Score = 66.2 bits (160), Expect = 2e-11
Identities = 32/110 (29%), Positives = 54/110 (49%)
Frame = +2
Query: 284 NGVVERKNRTLQEMARTMMQESSMAKHLWAEAINTAYYIHNIISIRPILEKTPYELCKGR 343
N +VERK+R + + R +M S + K+LW A+ A Y+ N + + + PY+
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAYLINXLPSPLLKGQCPYQFLNND 181
Query: 344 QPDISYFHPFGSTCYMLNTKEQLGKFDSKALKCYFLGYSERSKGFRIYNI 393
P + FG+ CY K D +A KC FLG+ + G+ + ++
Sbjct: 182 SPTLLDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKTGTXGYIVMDL 331
>TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprotein, partial
(4%)
Length = 528
Score = 50.4 bits (119), Expect(2) = 3e-11
Identities = 25/51 (49%), Positives = 34/51 (66%)
Frame = +3
Query: 958 HIEVKYHYIRDHVQKGTLSLEYVDTDHQWADIFTKPLAEDRFLFILENLNM 1008
+IE KYH++RD V KG +SL++ TD Q ADI TK L +RF + LN+
Sbjct: 69 NIETKYHFLRDQVTKGKISLKHCGTDLQVADIMTKGLKTERFRNMRAMLNV 221
Score = 35.4 bits (80), Expect(2) = 3e-11
Identities = 14/22 (63%), Positives = 18/22 (81%)
Frame = +2
Query: 939 DNTAAISLSKNPILHSRAKHIE 960
DN +AI L+KNP+ H R+KHIE
Sbjct: 5 DNKSAIDLAKNPVSHGRSKHIE 70
>BP063694
Length = 511
Score = 62.0 bits (149), Expect = 5e-10
Identities = 40/124 (32%), Positives = 60/124 (48%), Gaps = 3/124 (2%)
Frame = -2
Query: 122 IWLRRLGHASLRKISQLSK---LNLIRGLPRLKYSSEALCEACQKGKFTKKPFKAKNVVS 178
+W RLGH + I QL K L++ LP+ C +CQ K + F N +
Sbjct: 360 LWHSRLGHVNFDIIKQLHKHGCLDVSSILPK-----PICCTSCQMAKSKRLVFHDNNKRA 196
Query: 179 TTRPLELLHIDLFGPVKTESIGGKKYGLVIVDDYSRWTWVKFLRHKDETHTIFTNFITQV 238
+ L+L+H DL GP SI G Y ++ VDD+SR+TW L+ K + + F +
Sbjct: 195 SA-VLDLIHCDLRGPSPVASIDGFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLLRFKVFM 19
Query: 239 QKEF 242
+ F
Sbjct: 18 ENRF 7
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 57.8 bits (138), Expect = 9e-09
Identities = 36/114 (31%), Positives = 56/114 (48%), Gaps = 3/114 (2%)
Frame = +3
Query: 888 VTWSSKRQNTIALSTAEAEYVSAATCCTQTIWMKNHLEDYGLSLKKVPIYC---DNTAAI 944
V W S+ Q +ALSTAEAE+++A C + +WMKN L++ PI C A
Sbjct: 27 VAWPSRLQKCVALSTAEAEFIAATEACHELLWMKNFLQN--AWFHSHPILCCIVITKALF 200
Query: 945 SLSKNPILHSRAKHIEVKYHYIRDHVQKGTLSLEYVDTDHQWADIFTKPLAEDR 998
+L++ + + +RD + L LE + TD AD+ TK L ++
Sbjct: 201 TLARILLFIQDPSTLMFVIIGLRDVLNSKLLELEKIHTDDDGADMMTKSLPREK 362
>TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%)
Length = 550
Score = 57.4 bits (137), Expect = 1e-08
Identities = 30/82 (36%), Positives = 48/82 (57%), Gaps = 1/82 (1%)
Frame = +3
Query: 916 QTIWMKNHLEDYGL-SLKKVPIYCDNTAAISLSKNPILHSRAKHIEVKYHYIRDHVQKGT 974
+ +W+ L D + SL V IYCDN +A+ L+ N + H R ++IE+ H + V G
Sbjct: 21 EALWLTYALADLRIASLLLVVIYCDNRSALHLAANSVFHKRTENIEIDCHIV*VKVLFGI 200
Query: 975 LSLEYVDTDHQWADIFTKPLAE 996
L L +V + Q AD+FTK +++
Sbjct: 201 LHLLHVPSSDQVADVFTKTISQ 266
>AV427921
Length = 284
Score = 54.3 bits (129), Expect = 1e-07
Identities = 28/71 (39%), Positives = 39/71 (54%)
Frame = +1
Query: 280 TPQQNGVVERKNRTLQEMARTMMQESSMAKHLWAEAINTAYYIHNIISIRPILEKTPYEL 339
TPQQNGV ERKNRT+ M R+++ S + K EA+ + +I N + P E
Sbjct: 22 TPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQNMMPEEA 201
Query: 340 CKGRQPDISYF 350
GRQP + +F
Sbjct: 202 WSGRQPAVDHF 234
>TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, partial (4%)
Length = 670
Score = 52.8 bits (125), Expect = 3e-07
Identities = 23/73 (31%), Positives = 40/73 (54%)
Frame = +1
Query: 936 IYCDNTAAISLSKNPILHSRAKHIEVKYHYIRDHVQKGTLSLEYVDTDHQWADIFTKPLA 995
+YCD+ +A ++ N + H R KH+++ H +R+ +Q L + + Q ADI TKPL
Sbjct: 67 LYCDSQSARHIATNAVFHERTKHLDIDCHVVREKLQAKLFHLLPISSVDQTADILTKPLE 246
Query: 996 EDRFLFILENLNM 1008
F ++ L +
Sbjct: 247 SGPFSHLVSKLGV 285
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 50.4 bits (119), Expect = 1e-06
Identities = 34/99 (34%), Positives = 51/99 (51%), Gaps = 1/99 (1%)
Frame = -1
Query: 751 QKKYTLELLKKFNMSDCN-ISKTPMHPTCILEKEEVSSKVCQKLYRGMIGSLLYLTASRP 809
QK Y ++LKKF M++ IS T + +V Y+ +IGS+ YL R
Sbjct: 739 QKIYVDDILKKFKMTNSKYISTTIGGKEIEAGRRNGGKRVDSTYYKSLIGSVRYLNTVRS 560
Query: 810 DILFSVHLCARFQSDPRETHLTAVKRILKYLKGTTNLGL 848
DI+ V L +RF +P + H +R L+Y+KGT G+
Sbjct: 559 DIVCGVGLRSRFM-EP*DCH*QGAQRSLRYIKGTLKDGI 446
>TC19474 weakly similar to UP|Q9FJA1 (Q9FJA1) Similarity to retroelement pol
polyprotein, partial (3%)
Length = 517
Score = 37.7 bits (86), Expect(2) = 7e-06
Identities = 20/54 (37%), Positives = 32/54 (59%)
Frame = -2
Query: 939 DNTAAISLSKNPILHSRAKHIEVKYHYIRDHVQKGTLSLEYVDTDHQWADIFTK 992
DN AA+ + K RAKHIE+ +H++ D +S +V+++ Q D+FTK
Sbjct: 354 DNKAALHMPKI*F-SMRAKHIEIDFHFL*DRRLYLDISTRFVNSNDQLTDVFTK 196
Score = 29.6 bits (65), Expect(2) = 7e-06
Identities = 14/30 (46%), Positives = 18/30 (59%)
Frame = -1
Query: 883 LGSNLVTWSSKRQNTIALSTAEAEYVSAAT 912
L NL++W SK +A S AEAE+ AT
Sbjct: 517 LSDNLISWKSKETIIVARSRAEAEFRVMAT 428
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.135 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,342,478
Number of Sequences: 28460
Number of extensions: 239112
Number of successful extensions: 1114
Number of sequences better than 10.0: 61
Number of HSP's better than 10.0 without gapping: 1104
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1111
length of query: 1017
length of database: 4,897,600
effective HSP length: 99
effective length of query: 918
effective length of database: 2,080,060
effective search space: 1909495080
effective search space used: 1909495080
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0166.5