BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0166.4
(721 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF177549 34 0.072
TC12762 33 0.16
TC11734 similar to UP|WR49_ARATH (Q9FHR7) Probable WRKY transcri... 33 0.21
TC14393 similar to UP|Q93YA8 (Q93YA8) Calcium binding protein, p... 32 0.27
AV423371 32 0.36
TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (... 32 0.36
AV781265 31 0.61
TC15010 weakly similar to UP|SR40_YEAST (P32583) Suppressor prot... 31 0.79
TC19569 similar to UP|AAR11301 (AAR11301) Lectin-like receptor k... 30 1.0
TC15534 weakly similar to GB|AAO63420.1|28950993|BT005356 At5g28... 30 1.0
TC12483 weakly similar to UP|Q40552 (Q40552) Pistil extensin lik... 30 1.0
TC14083 similar to UP|CRTC_RICCO (P93508) Calreticulin precursor... 30 1.4
TC8582 similar to UP|Q8LJS2 (Q8LJS2) Nucleolar histone deacetyla... 30 1.4
BP048490 30 1.4
TC13398 weakly similar to UP|Q8AWB2 (Q8AWB2) Keratin gamma3, par... 30 1.4
TC8142 UP|BAD11133 (BAD11133) Glutamate-rich protein, complete 30 1.4
AV422838 30 1.8
TC19679 similar to UP|Q9SSX8 (Q9SSX8) WIZZ, partial (23%) 30 1.8
BP034655 30 1.8
TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%) 29 2.3
>BF177549
Length = 440
Score = 34.3 bits (77), Expect = 0.072
Identities = 14/24 (58%), Positives = 22/24 (91%)
Frame = +2
Query: 698 QESSRRMEDMMKFLIEHLPSSSKP 721
+ESS +ME +MK+L+++LPS+SKP
Sbjct: 11 RESSSKMEAIMKYLMDNLPSTSKP 82
>TC12762
Length = 865
Score = 33.1 bits (74), Expect = 0.16
Identities = 20/67 (29%), Positives = 36/67 (52%)
Frame = +3
Query: 201 LEYVRMLRRNGDKITLEEFVRTLPESPPEMPTRRSKKTTSKPSDPKGKEILIEEPKRKKA 260
+E+ +ML +G+ I L V PE + ++ SKP+DPK KE +E + +KA
Sbjct: 105 VEFAKMLDDSGNVIVLGNVVFLRPEQVAKSIESLINQSISKPNDPKRKE--LEHMENQKA 278
Query: 261 ASNEETE 267
+++ +
Sbjct: 279 MIDDKAK 299
>TC11734 similar to UP|WR49_ARATH (Q9FHR7) Probable WRKY transcription
factor 49 (WRKY DNA-binding protein 49), partial (28%)
Length = 1009
Score = 32.7 bits (73), Expect = 0.21
Identities = 34/153 (22%), Positives = 63/153 (40%), Gaps = 2/153 (1%)
Frame = +3
Query: 293 IQNVLQVIRESSEAEESTDSDEVPLVKRR--KLPLKGPLQQKEADSQQASDPASEAQRER 350
I+N L V +++ E + S V +++R K+ K L+ K + D R+
Sbjct: 192 IENALSVT--TNKTEHFSASARVSILERNLSKIENKYTLKIKCFGNGMGDD--GYKWRKY 359
Query: 351 RSKSTSDPARAAQLTRTTSIRSPSKVITESINSDSALVVIPEQPLHISTSMPTLTQTAAT 410
KS + R T+ R +K E N D ++I + LH+ + P
Sbjct: 360 GQKSIKNSPNPRSYYRCTNPRCSAKKQVERSNEDPETLIITYEGLHLHFAYPCFLVGQPQ 539
Query: 411 QPETQAETPDLSFTSLENTATSRQRQVTISEAE 443
Q ++ FTS + A ++ + ++EA+
Sbjct: 540 QSQSHPPVKKSKFTSSQVQAQAQAQDFIVNEAQ 638
>TC14393 similar to UP|Q93YA8 (Q93YA8) Calcium binding protein, partial
(85%)
Length = 786
Score = 32.3 bits (72), Expect = 0.27
Identities = 35/151 (23%), Positives = 61/151 (40%), Gaps = 9/151 (5%)
Frame = +1
Query: 297 LQVIRESSEAEESTDSDEVPLVKRRKLPLKGPLQQKEA-DSQQASDPASEAQRERRSKST 355
L+ +E + E T++++ P +K P+ PL + S PAS RS ST
Sbjct: 46 LKTKQEWQQTETETETEK-PTLK----PVSNPLSTSTTWKNSSESSPASTPTATARSPST 210
Query: 356 SDPARAAQLTRTTSIRSPSKVITESINSDSALVVIPEQPLHISTSMPTLTQTAATQPETQ 415
S +A RS S S + +A + P P + + T+ ++T P +
Sbjct: 211 SSTTSSAPSDPAFHRRSSSASWWTSTATTTASSISPSSPPSAAPTPRTVVPPSSTTPSSS 390
Query: 416 AETPDLSFTSLENTA--------TSRQRQVT 438
+ + L ++A T+R+R T
Sbjct: 391 TTRTRTASSPLRSSARC*TASE*TARRRNAT 483
>AV423371
Length = 437
Score = 32.0 bits (71), Expect = 0.36
Identities = 23/101 (22%), Positives = 48/101 (46%), Gaps = 3/101 (2%)
Frame = +3
Query: 222 TLPESPPEMPTRRSKKTTSKPSDPKGKEILIEEPKRKKAASNEETEDD---DVPLDKRRK 278
T+ E+ RS ++ + G+E++IE PK+ K A+ E + + + ++ R+
Sbjct: 51 TVSSLEAELDKTRSDIVLAQAKENDGREMIIELPKKLKEAAEEANQANLHAEAAREELRR 230
Query: 279 VIFEEEDEQDDHEIIQNVLQVIRESSEAEESTDSDEVPLVK 319
V E E + +Q+ L ++ EA +++ + VK
Sbjct: 231 VKEEAEQAKAGASTMQSKLLAAQKEIEAARASERLALSAVK 353
>TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (SPBC3D6.14C
protein) (Fragment), partial (15%)
Length = 543
Score = 32.0 bits (71), Expect = 0.36
Identities = 21/67 (31%), Positives = 31/67 (45%)
Frame = +3
Query: 249 EILIEEPKRKKAASNEETEDDDVPLDKRRKVIFEEEDEQDDHEIIQNVLQVIRESSEAEE 308
EI + P + NEE EDDD D E+ED+ DD + + + + + EE
Sbjct: 207 EIYPDGPPKPVQTDNEEEEDDDEEEDD------EDEDDDDDDDDDDDGEEEEDDDDDDEE 368
Query: 309 STDSDEV 315
S+EV
Sbjct: 369 EEGSEEV 389
>AV781265
Length = 494
Score = 31.2 bits (69), Expect = 0.61
Identities = 22/84 (26%), Positives = 41/84 (48%), Gaps = 7/84 (8%)
Frame = -1
Query: 224 PESPPEMPTRRSKKTTSKPSDPKGKEILIEEPKRKKAASNEETEDDDVPLDKRRKVIFEE 283
PE+ + R K TS+PS + ++I I + + + E E+D+ +D K I+EE
Sbjct: 320 PETLTKWQARLKNKETSRPSSSRARDI-IADAEEEVTYDVEIEEEDEAAID---KYIYEE 153
Query: 284 -------EDEQDDHEIIQNVLQVI 300
+EQ D+E + +++
Sbjct: 152 MYKDDESSEEQGDNEFY*GLRRIV 81
>TC15010 weakly similar to UP|SR40_YEAST (P32583) Suppressor protein SRP40,
partial (5%)
Length = 1439
Score = 30.8 bits (68), Expect = 0.79
Identities = 17/44 (38%), Positives = 24/44 (53%), Gaps = 2/44 (4%)
Frame = +2
Query: 542 GKAHELRKQLSREKRLRVHDASERAYEQRRRVLHRVCA--PLRR 583
G+A ELR++L + LR + R+ R LHR C PL+R
Sbjct: 203 GRAQELRRELRQRLELRPLE*GARSLRMRGETLHRRCCLLPLQR 334
>TC19569 similar to UP|AAR11301 (AAR11301) Lectin-like receptor kinase 1;1,
partial (16%)
Length = 486
Score = 30.4 bits (67), Expect = 1.0
Identities = 27/108 (25%), Positives = 43/108 (39%), Gaps = 14/108 (12%)
Frame = +1
Query: 329 LQQKEADSQQASDPASEAQRERRSKSTSD------PARAAQLTR------TTSIRSPSKV 376
L + S S P +Q+ S STS P ++T +TS +SP+
Sbjct: 37 LHTSSSSSSSYSPPPPPSQQPTHSPSTSPTSTTTPPPSPTKVTENPPTAPSTSTKSPTSS 216
Query: 377 ITESINSDSALVVIPEQPLHISTSMPTLTQTA--ATQPETQAETPDLS 422
+E + + +QP H TS P + +T+P T +P S
Sbjct: 217 ASEEPSPPNPSTSTTQQPKHSPTSPPASPSPSPPSTKPPTAMASPSTS 360
>TC15534 weakly similar to GB|AAO63420.1|28950993|BT005356 At5g28040
{Arabidopsis thaliana;}, partial (8%)
Length = 937
Score = 30.4 bits (67), Expect = 1.0
Identities = 29/124 (23%), Positives = 48/124 (38%)
Frame = -3
Query: 239 TSKPSDPKGKEILIEEPKRKKAASNEETEDDDVPLDKRRKVIFEEEDEQDDHEIIQNVLQ 298
T PS PK + K+K+ + E+ P EEED+Q ++ +
Sbjct: 932 TGPPSAPKP*SQITMAQKQKRPSPLED------PPTASSSSESEEEDDQPLSQVKHAAAE 771
Query: 299 VIRESSEAEESTDSDEVPLVKRRKLPLKGPLQQKEADSQQASDPASEAQRERRSKSTSDP 358
SSE E S++ +E P K + SD A+++ E + S P
Sbjct: 770 EEELSSEEEGSSEEEEEDETTAPAPPAATTSHTKPPQPEPESDSATQSDSESDTDSDQAP 591
Query: 359 ARAA 362
+ +A
Sbjct: 590 SASA 579
>TC12483 weakly similar to UP|Q40552 (Q40552) Pistil extensin like protein
(Fragment), partial (6%)
Length = 534
Score = 30.4 bits (67), Expect = 1.0
Identities = 23/93 (24%), Positives = 39/93 (41%), Gaps = 4/93 (4%)
Frame = +2
Query: 392 EQPLHISTSMPTLTQTAATQPETQAETPDLSFTSLENTATSRQRQVTISEAEHMDESDHS 451
+QP + +S+ +T + + P LS + + Q T + H ++H
Sbjct: 119 KQPFPLPSSLQRNPKTLPPPFSMKPQNPSLSIHEIRPPNNHHRHQTTTC-SHHRPPNNHP 295
Query: 452 GIEK----DHEIDSIHSEQTRPDSSNASSSNAT 480
K H + SEQ RPD S+ SS +A+
Sbjct: 296 RHHKASICPHMQADLRSEQKRPDRSSCSSLSAS 394
>TC14083 similar to UP|CRTC_RICCO (P93508) Calreticulin precursor, partial
(92%)
Length = 1693
Score = 30.0 bits (66), Expect = 1.4
Identities = 23/96 (23%), Positives = 38/96 (38%)
Frame = +1
Query: 219 FVRTLPESPPEMPTRRSKKTTSKPSDPKGKEILIEEPKRKKAASNEETEDDDVPLDKRRK 278
F L PE + +++T K D + E KR++ S E+ D D
Sbjct: 1099 FDNVLITDDPEYAKQVAEETWGKQKDAEKAAFEEAEKKREEEESKEDPADSDA------- 1257
Query: 279 VIFEEEDEQDDHEIIQNVLQVIRESSEAEESTDSDE 314
EDE+D E + ++ E+S D++E
Sbjct: 1258 -----EDEEDTDEASHDSDDAESKTEAGEDSADANE 1350
>TC8582 similar to UP|Q8LJS2 (Q8LJS2) Nucleolar histone deacetylase
HD2-P39, partial (65%)
Length = 1341
Score = 30.0 bits (66), Expect = 1.4
Identities = 31/134 (23%), Positives = 56/134 (41%), Gaps = 8/134 (5%)
Frame = +1
Query: 217 EEFVRTLPESPPEMPTRRSKKT-TSKPS---DPKGKEILIEEPKRKKAASNEETEDDDVP 272
EE + PE T +K T+KP+ P K++ I +PK+ + ++ + DV
Sbjct: 403 EEELMEQENGKPETKTEDAKVAKTAKPAAAAGPSAKQVKIVDPKKDEEEADLDVSSPDVS 582
Query: 273 LDKRRKVIFEEEDEQDDHEIIQNVLQVIRESSEAEESTDSDEVPLVKRRKLP----LKGP 328
+I +ED D+ + + S+ EE T + + + +K P K P
Sbjct: 583 -GYEDDLISADEDSDDESD----------DESDDEEETPTPAKKVDQGKKRPNESASKTP 729
Query: 329 LQQKEADSQQASDP 342
+ K+A + P
Sbjct: 730 VSGKKAKNATPEKP 771
>BP048490
Length = 452
Score = 30.0 bits (66), Expect = 1.4
Identities = 21/83 (25%), Positives = 37/83 (44%), Gaps = 5/83 (6%)
Frame = +3
Query: 302 ESSEAEESTDSDEVPLVKRRKLPLKGPLQQK-----EADSQQASDPASEAQRERRSKSTS 356
ESS+ E S SDE K + P K ++D + +SD SE ++++ S
Sbjct: 18 ESSDDESSESSDEENDSKPATTTVAKPSAVKKSASSDSDDEDSSDSDSEVKKDKMDVDES 197
Query: 357 DPARAAQLTRTTSIRSPSKVITE 379
D + + S ++P K + +
Sbjct: 198 DSSEDSDEEDEKSTKTPQKKVKD 266
>TC13398 weakly similar to UP|Q8AWB2 (Q8AWB2) Keratin gamma3, partial (4%)
Length = 526
Score = 30.0 bits (66), Expect = 1.4
Identities = 18/72 (25%), Positives = 35/72 (48%)
Frame = +3
Query: 538 RWSEGKAHELRKQLSREKRLRVHDASERAYEQRRRVLHRVCAPLRRAMAERQYVVSECTS 597
+W E K H L + + K+ +A ER YEQ++ ++ + A + +A ++ + +
Sbjct: 219 QWVEIKQHFLDLETALTKKHEELEAREREYEQKQSEMNTLLAERKEEVARKEQDLLDWLQ 398
Query: 598 EDAEMIPAEAAE 609
E + A AE
Sbjct: 399 ELKDAAVASIAE 434
>TC8142 UP|BAD11133 (BAD11133) Glutamate-rich protein, complete
Length = 999
Score = 30.0 bits (66), Expect = 1.4
Identities = 32/131 (24%), Positives = 54/131 (40%), Gaps = 3/131 (2%)
Frame = +1
Query: 222 TLPESPPEMPTRRSKKTTSKPSD--PKGKEILIEEPKRKKAASNEETE-DDDVPLDKRRK 278
T+PE PE P +P+ P E EEPK + +ETE + + P+ +
Sbjct: 169 TIPE--PEQPAATEVAAPEQPAAEVPAPAEPATEEPKEETTEEPKETETETEAPVAPETQ 342
Query: 279 VIFEEEDEQDDHEIIQNVLQVIRESSEAEESTDSDEVPLVKRRKLPLKGPLQQKEADSQQ 338
E E+++ E +V E+ + EE T+ + + P + A ++
Sbjct: 343 DPLEVENKEVTEEAKPE--EVKPETEKTEEKTEE------PKTEEPAATTETESAAPVEE 498
Query: 339 ASDPASEAQRE 349
+ PA EA E
Sbjct: 499 ENKPAEEAPVE 531
>AV422838
Length = 448
Score = 29.6 bits (65), Expect = 1.8
Identities = 17/47 (36%), Positives = 26/47 (55%)
Frame = +3
Query: 340 SDPASEAQRERRSKSTSDPARAAQLTRTTSIRSPSKVITESINSDSA 386
S+PAS + S + +P AA +TS RSP+ +T S +S S+
Sbjct: 195 SNPASSSSPPTASSGSPNPPTAALPPLSTSPRSPTSSLTRSPSSPSS 335
>TC19679 similar to UP|Q9SSX8 (Q9SSX8) WIZZ, partial (23%)
Length = 605
Score = 29.6 bits (65), Expect = 1.8
Identities = 24/116 (20%), Positives = 48/116 (40%)
Frame = +1
Query: 193 KKDNPASILEYVRMLRRNGDKITLEEFVRTLPESPPEMPTRRSKKTTSKPSDPKGKEILI 252
K+++ +++E ++ + K+T L E T RS T ++P KE++
Sbjct: 244 KEESNGALVEELKRVSAENKKLT-----EMLTEMCENYNTLRSNLTEYMRNNPDNKEVIS 408
Query: 253 EEPKRKKAASNEETEDDDVPLDKRRKVIFEEEDEQDDHEIIQNVLQVIRESSEAEE 308
KRK +SN + V + EE ++ E I+ + + +E +
Sbjct: 409 TSKKRKSESSNNNSNPLGVNGNSESSSTDEETCKKPREETIKAKVSKVYHRTEPSD 576
>BP034655
Length = 517
Score = 29.6 bits (65), Expect = 1.8
Identities = 16/68 (23%), Positives = 34/68 (49%)
Frame = +1
Query: 251 LIEEPKRKKAASNEETEDDDVPLDKRRKVIFEEEDEQDDHEIIQNVLQVIRESSEAEEST 310
L ++ + ++ EE E+++ ++ + EE+DE+D+ E V R S ++
Sbjct: 130 LSDDDEEEEEEEEEEEEEEEEEEEEEEEEEDEEDDEEDEDEDYIPVAPSHRVSPNVHQNP 309
Query: 311 DSDEVPLV 318
SD V ++
Sbjct: 310 TSDSVEVI 333
>TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%)
Length = 1008
Score = 29.3 bits (64), Expect = 2.3
Identities = 22/81 (27%), Positives = 34/81 (41%)
Frame = -2
Query: 242 PSDPKGKEILIEEPKRKKAASNEETEDDDVPLDKRRKVIFEEEDEQDDHEIIQNVLQVIR 301
P G E E+ + + E+ +DDD E++DE+DD + V
Sbjct: 464 PGAGAGPEENGEDEEDEDGEDQEDDDDDD-----------EDDDEEDDGGEDEEEEGVEE 318
Query: 302 ESSEAEESTDSDEVPLVKRRK 322
E +E EE + DE L +K
Sbjct: 317 EDNEDEEEDEEDEEALQPPKK 255
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.311 0.127 0.347
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,455,747
Number of Sequences: 28460
Number of extensions: 113288
Number of successful extensions: 760
Number of sequences better than 10.0: 57
Number of HSP's better than 10.0 without gapping: 746
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 756
length of query: 721
length of database: 4,897,600
effective HSP length: 97
effective length of query: 624
effective length of database: 2,136,980
effective search space: 1333475520
effective search space used: 1333475520
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0166.4