BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0162b.5
(220 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC12852 similar to UP|Q9FQD9 (Q9FQD9) Glutathione S-transferase ... 173 2e-44
TC14695 similar to UP|Q9FQE5 (Q9FQE5) Glutathione S-transferase ... 168 1e-43
TC15244 similar to UP|O49235 (O49235) 2,4-D inducible glutathion... 162 5e-41
TC11872 similar to UP|GTX6_SOYBN (P32110) Probable glutathione S... 149 5e-37
TC9339 similar to UP|Q9FQE3 (Q9FQE3) Glutathione S-transferase G... 141 8e-35
BG662417 140 2e-34
TC11978 similar to UP|GTXA_TOBAC (P25317) Probable glutathione S... 134 2e-32
BF177888 130 2e-31
BP073118 120 2e-28
AU251792 119 5e-28
AV423845 96 4e-21
TC10564 similar to UP|Q9FQD3 (Q9FQD3) Glutathione S-transferase ... 59 7e-10
TC12677 similar to UP|Q9FQD6 (Q9FQD6) Glutathione S-transferase ... 39 7e-04
TC11227 similar to UP|Q9FQ95 (Q9FQ95) In2-1 protein, partial (54%) 36 0.006
AW163994 33 0.050
TC10184 similar to UP|DCAM_PHANI (Q96471) S-adenosylmethionine d... 28 1.2
AV421359 28 1.6
TC10843 26 6.2
TC13762 similar to UP|Q9LDE2 (Q9LDE2) F10B6.5 (T5E21.17) (At1g14... 25 8.0
>TC12852 similar to UP|Q9FQD9 (Q9FQD9) Glutathione S-transferase GST 19 ,
partial (97%)
Length = 909
Score = 173 bits (439), Expect = 2e-44
Identities = 85/152 (55%), Positives = 111/152 (72%)
Frame = +2
Query: 5 EVKLVSFWASSYGKKVEWALKLKGVEYEYVEEDIFNKSNLLLELNPVHKKVPVLVHGNKP 64
EVKL++ S ++EWAL++KGVEYEY++ED+ NKS LLL+ NPVHKKVPVL+H KP
Sbjct: 65 EVKLIATPQSFPCARIEWALRIKGVEYEYIQEDLANKSPLLLQSNPVHKKVPVLLHDGKP 244
Query: 65 IAESLIILEYIDETWKHQYPLFPQDPYERALARFWAASIEPKLLKAAWVELFTSGDEQEN 124
IAESL+ILEYIDETWK + PL P DPYERALARFWA I+ K + W G+E+
Sbjct: 245 IAESLVILEYIDETWK-ENPLLPLDPYERALARFWARFIDEKCVIGVWGACVAQGEEKGK 421
Query: 125 AMKEATEGLEMVEEQIKGKKFFGGITLATLTL 156
A++ A + LE +E+QI+G K+FGG + L +
Sbjct: 422 AIETALDSLEFLEKQIQGNKYFGGERIGYLDI 517
>TC14695 similar to UP|Q9FQE5 (Q9FQE5) Glutathione S-transferase GST 13 ,
partial (97%)
Length = 1338
Score = 168 bits (426), Expect(2) = 1e-43
Identities = 80/155 (51%), Positives = 112/155 (71%)
Frame = +3
Query: 2 ESKEVKLVSFWASSYGKKVEWALKLKGVEYEYVEEDIFNKSNLLLELNPVHKKVPVLVHG 61
E EV+L+ WAS + +V+ ALKLKGV Y Y EED+ NKS LL+ NPV+KKVPVLVH
Sbjct: 84 EHGEVRLLGKWASPFSNRVDLALKLKGVPYNYSEEDLANKSADLLKYNPVYKKVPVLVHN 263
Query: 62 NKPIAESLIILEYIDETWKHQYPLFPQDPYERALARFWAASIEPKLLKAAWVELFTSGDE 121
P+AESL+ILEYIDETWK+ P PQDPYERA+ARFW+ +++ K+L W ++
Sbjct: 264 GNPLAESLVILEYIDETWKNS-PFLPQDPYERAVARFWSKTLDDKILPPIWNACWSDEKG 440
Query: 122 QENAMKEATEGLEMVEEQIKGKKFFGGITLATLTL 156
+E A++EA E L+++++Q+K +FFGG T+ + +
Sbjct: 441 REKAVEEALEALKLLQDQLKDNRFFGGETIGLVDI 545
Score = 23.5 bits (49), Expect(2) = 1e-43
Identities = 18/46 (39%), Positives = 23/46 (49%)
Frame = +1
Query: 164 GFLFGRKLGLLRYLIH*NIQPPLHG*PIFSSTL*SRTLGPQETKCL 209
GFL+ +KL L+ N L+G S+TL*SR E CL
Sbjct: 568 GFLYYKKLQG*SCLLLRNFPSCLNGAKNLSTTL*SRKAFLPEMNCL 705
>TC15244 similar to UP|O49235 (O49235) 2,4-D inducible glutathione
S-transferase , complete
Length = 1309
Score = 162 bits (409), Expect = 5e-41
Identities = 82/168 (48%), Positives = 109/168 (64%)
Frame = +3
Query: 5 EVKLVSFWASSYGKKVEWALKLKGVEYEYVEEDIFNKSNLLLELNPVHKKVPVLVHGNKP 64
EV L+ FWAS +G +V AL KG+ YEY EED+ NKS LLL+ NPVHKK+PVL+H KP
Sbjct: 54 EVVLLDFWASPFGMRVRIALAEKGINYEYKEEDLRNKSPLLLQSNPVHKKIPVLIHNGKP 233
Query: 65 IAESLIILEYIDETWKHQYPLFPQDPYERALARFWAASIEPKLLKAAWVELFTSGDEQEN 124
IAESLI ++YIDE W PL P DPY+RA ARFWA ++ K+ + ++ +E+E
Sbjct: 234 IAESLIAVQYIDEVWNDASPLLPSDPYQRAQARFWADYVDKKIYEIGRNIIWKKNEEREA 413
Query: 125 AMKEATEGLEMVEEQIKGKKFFGGITLATLTLQLVGSLTGFLFGRKLG 172
A KE + L+++EEQ+ K +FGG L + + LV T F LG
Sbjct: 414 AKKEFIDCLKLLEEQLGDKTYFGGDKLGYVDIALVPFYTWFKGYETLG 557
>TC11872 similar to UP|GTX6_SOYBN (P32110) Probable glutathione
S-transferase (Heat shock protein 26A) (G2-4) ,
complete
Length = 968
Score = 149 bits (375), Expect = 5e-37
Identities = 76/146 (52%), Positives = 105/146 (71%), Gaps = 1/146 (0%)
Frame = +2
Query: 4 KEVKLVSFWASSYGKKVEWALKLKGVEYEYVEEDIFNKSNLLLELNPVHKKVPVLVHGNK 63
+ V+L+ S + +V+ AL LKGVEYE+VE+ KS+LLL+ NPVHKKVPV VH +K
Sbjct: 38 ENVQLLGIVGSPFVCRVQIALNLKGVEYEFVEQIFGQKSDLLLKYNPVHKKVPVFVHNDK 217
Query: 64 PIAESLIILEYIDETWKHQYPLFPQDPYERALARFWAASIEPKLLKAAWVELFTSGD-EQ 122
PIAESL+ILEYIDETWK P+ P DPY+RALARFW+ I+ K++ W +F + E+
Sbjct: 218 PIAESLVILEYIDETWKSN-PILPSDPYKRALARFWSKFIDDKVIDTTWKAVFILEEAER 394
Query: 123 ENAMKEATEGLEMVEEQIKGKKFFGG 148
E ++E +E L+ +E ++K +KFFGG
Sbjct: 395 EKIIEELSEALQYLENELK-EKFFGG 469
>TC9339 similar to UP|Q9FQE3 (Q9FQE3) Glutathione S-transferase GST 15
(Fragment) , partial (44%)
Length = 981
Score = 141 bits (356), Expect = 8e-35
Identities = 72/152 (47%), Positives = 100/152 (65%), Gaps = 4/152 (2%)
Frame = +3
Query: 4 KEVKLVSFWASSYGKKVEWALKLKGVEYEYVEEDIFNKSNLLLELNPVHKKVPVLVHGNK 63
+++++ FW S + +V W LKLKG+ +EY EED +NKS LL+ NPVHKK PVLVH K
Sbjct: 12 EQIEVHGFWYSPFTFRVLWTLKLKGIAFEYFEEDRYNKSPQLLQYNPVHKKTPVLVHDGK 191
Query: 64 PIAESLIILEYIDETWKHQYPLFPQDPYERALARFWAASIEPKLLKAAWVELF--TSGDE 121
P+ ES+II+EYIDE W Q PL P DPYE+A+ARFW ++ ++ + F +E
Sbjct: 192 PLCESMIIVEYIDELWP-QNPLVPSDPYEKAVARFWVRYVD-DMMSPVLPQFFGRFGSEE 365
Query: 122 QENAMKEATEGLEMVEEQIKG--KKFFGGITL 151
Q+ +K+ E E++E+Q G KKF GG TL
Sbjct: 366 QDKVIKDIWERFEVIEDQYLGDHKKFLGGDTL 461
>BG662417
Length = 484
Score = 140 bits (352), Expect = 2e-34
Identities = 79/157 (50%), Positives = 100/157 (63%), Gaps = 3/157 (1%)
Frame = +2
Query: 7 KLVSFWASSYGKKVEWALKLKGVEYEYVEEDIFNKSNLLLELNPVHKKVPVLVHGNKPIA 66
KL FW S + +V W LK+KG+ YEY+EED NK+ LLE +PVHKKVPV VH KP+
Sbjct: 17 KLHGFWFSPFVLRVVWTLKVKGIPYEYIEEDPANKTPQLLEYHPVHKKVPVFVHDGKPVC 196
Query: 67 ESLIILEYIDETWKHQYPLFPQDPYERALARFWAASIEPKLLKAAWVELFTSGD--EQEN 124
ES+II+EYIDE W Q PL P DPYERA+ARFW I+ ++ A + L S D E+E
Sbjct: 197 ESIIIVEYIDEIWS-QNPLVPADPYERAIARFWVRYID-DMISAVLLPLCRSNDIAEREE 370
Query: 125 AMKEATEGLEMVEEQIKG-KKFFGGITLATLTLQLVG 160
+KE ++EEQ G +K F TL TL L+G
Sbjct: 371 IIKEIWARFRVIEEQCSGDQKNFLEETLLTLWT*LLG 481
>TC11978 similar to UP|GTXA_TOBAC (P25317) Probable glutathione
S-transferase parA (Auxin-regulated protein parA)
(STR246C protein) , partial (65%)
Length = 454
Score = 134 bits (336), Expect = 2e-32
Identities = 66/145 (45%), Positives = 91/145 (62%)
Frame = +2
Query: 1 MESKEVKLVSFWASSYGKKVEWALKLKGVEYEYVEEDIFNKSNLLLELNPVHKKVPVLVH 60
M +V L+ FW SSYG +V+ AL KG+ YE +ED KS+LLLE+NPVHK +PVL+H
Sbjct: 20 MAENKVVLLDFWPSSYGMRVKIALAEKGISYECKQEDFQAKSSLLLEMNPVHKMIPVLIH 199
Query: 61 GNKPIAESLIILEYIDETWKHQYPLFPQDPYERALARFWAASIEPKLLKAAWVELFTSGD 120
KPI ESL I+EYIDE W H+ L P DPY+R+ ARFW I+ + G+
Sbjct: 200 NGKPICESLNIVEYIDEAWNHKPSLLPADPYKRSQARFWGDYIDKNVYSIGKRVWTGKGE 379
Query: 121 EQENAMKEATEGLEMVEEQIKGKKF 145
+QE K+ E L+++E ++ K +
Sbjct: 380 DQEEGKKKFIECLKILEGELGDKPY 454
>BF177888
Length = 420
Score = 130 bits (326), Expect = 2e-31
Identities = 67/136 (49%), Positives = 89/136 (65%), Gaps = 2/136 (1%)
Frame = +2
Query: 5 EVKLVSFWASSYGKKVEWALKLKGVEYEYVEEDIFNKSNLLLELNPVHKKVPVLVHGNKP 64
++KL FW S + +V W LKLKG+ +EY+EED FNKS LL+ NPVHKK PV VH KP
Sbjct: 17 DLKLHGFWYSPFTFRVLWTLKLKGIPFEYIEEDRFNKSPQLLQYNPVHKKTPVFVHDGKP 196
Query: 65 IAESLIILEYIDETWKHQYPLFPQDPYERALARFWAASIEPKLLKAAWVELFTSGD--EQ 122
I ES+II+EYIDE W Q PL P DP+ +A+ARFW +E ++ A LF S E+
Sbjct: 197 ICESMIIVEYIDELWT-QNPLVPSDPHVKAVARFWVRYVE-DMISAVASPLFRSNGSAER 370
Query: 123 ENAMKEATEGLEMVEE 138
E +K+ E ++E+
Sbjct: 371 EKVIKDIWERFGVIED 418
>BP073118
Length = 495
Score = 120 bits (301), Expect = 2e-28
Identities = 55/93 (59%), Positives = 69/93 (74%)
Frame = +1
Query: 12 WASSYGKKVEWALKLKGVEYEYVEEDIFNKSNLLLELNPVHKKVPVLVHGNKPIAESLII 71
W S + +V W LKLKG+ +EY+EED +NKS LL+ NPVHKK PVLVH KP+ ES+II
Sbjct: 25 WYSPFTCRVLWTLKLKGIAFEYLEEDRYNKSPQLLQYNPVHKKTPVLVHDGKPLCESMII 204
Query: 72 LEYIDETWKHQYPLFPQDPYERALARFWAASIE 104
+EYIDE W Q PL P DPYE+A+ARFW ++
Sbjct: 205 VEYIDELWP-QNPLVPSDPYEKAVARFWVRYVD 300
>AU251792
Length = 363
Score = 119 bits (297), Expect = 5e-28
Identities = 54/105 (51%), Positives = 72/105 (68%)
Frame = +2
Query: 2 ESKEVKLVSFWASSYGKKVEWALKLKGVEYEYVEEDIFNKSNLLLELNPVHKKVPVLVHG 61
+S +VKL+ W S + AL +K V+YE++ E KS LLL+ NPVH+KVPV H
Sbjct: 26 KSDDVKLLGAWQSPVTLRARIALNIKSVDYEFIAESFEPKSQLLLQSNPVHQKVPVFFHK 205
Query: 62 NKPIAESLIILEYIDETWKHQYPLFPQDPYERALARFWAASIEPK 106
+KPI ESL+I++Y+DE W P+ P DPYERA+ARFWAA I+ K
Sbjct: 206 DKPICESLVIVQYVDEVWSSASPILPSDPYERAIARFWAAFIDDK 340
>AV423845
Length = 259
Score = 96.3 bits (238), Expect = 4e-21
Identities = 45/77 (58%), Positives = 59/77 (76%)
Frame = +3
Query: 3 SKEVKLVSFWASSYGKKVEWALKLKGVEYEYVEEDIFNKSNLLLELNPVHKKVPVLVHGN 62
S E+KL+S W S + +V AL LKG++YE +EE + KS+LLL+ NPVHKK+PVL+H +
Sbjct: 9 SNELKLLSGWFSPFAMRVRIALNLKGLDYEVLEETLNPKSDLLLQSNPVHKKIPVLIHKD 188
Query: 63 KPIAESLIILEYIDETW 79
KPI ES II+EYIDE W
Sbjct: 189KPICESAIIVEYIDEVW 239
>TC10564 similar to UP|Q9FQD3 (Q9FQD3) Glutathione S-transferase GST 25 ,
partial (53%)
Length = 500
Score = 58.9 bits (141), Expect = 7e-10
Identities = 41/105 (39%), Positives = 64/105 (60%), Gaps = 3/105 (2%)
Frame = +1
Query: 2 ESKEVKLVSFWASSYGKKVEWALKLKGVEYEYVEEDIF--NKSN-LLLELNPVHKKVPVL 58
+ ++KL S+W SS +V AL LKG++YEY ++ +SN L+LNPV VPVL
Sbjct: 139 QDNKLKLYSYWRSSCSFRVRIALNLKGLKYEYKPVNLLKGEQSNPEFLKLNPV-GCVPVL 315
Query: 59 VHGNKPIAESLIILEYIDETWKHQYPLFPQDPYERALARFWAASI 103
V G I +S I+ Y+++ + + +PL P+D +RA+ F A +I
Sbjct: 316 VDGPAVIFDSFAIIMYLEDKYPN-HPLLPRDINQRAI-NFQATNI 444
>TC12677 similar to UP|Q9FQD6 (Q9FQD6) Glutathione S-transferase GST 22
(Fragment) , complete
Length = 876
Score = 38.9 bits (89), Expect = 7e-04
Identities = 44/161 (27%), Positives = 74/161 (45%), Gaps = 17/161 (10%)
Frame = +3
Query: 5 EVKLVSFWASSYG--KKVEWALKLKGVEYEYVEEDIF---NKSNLLLELNPVHKKVPVLV 59
E +V + +Y K+V L K +E+E V DIF +K L++ P ++PV+
Sbjct: 24 ENMVVKVYGPAYASPKRVMVCLIEKEIEFETVPVDIFKREHKDPEYLKIQP-FGELPVIQ 200
Query: 60 HGNKPIAESLIILEYIDETWKHQ-YPLFPQDPYERALARFW----AASIEPKLLKAAWVE 114
G+ + ES I+ Y E +K+Q L + ER L W A S +P +
Sbjct: 201 DGDYTLYESRAIIRYYAEKYKNQGTELLGKTIEERGLVEQWLDVEAHSFQPAIHNLVNHV 380
Query: 115 LF-----TSGDEQ--ENAMKEATEGLEMVEEQIKGKKFFGG 148
LF T D + E + ++ + L++ EE++ K+ G
Sbjct: 381 LFAPILGTPSDPKVIEESDEKLVKVLDIYEERLSKSKYLAG 503
>TC11227 similar to UP|Q9FQ95 (Q9FQ95) In2-1 protein, partial (54%)
Length = 446
Score = 35.8 bits (81), Expect = 0.006
Identities = 24/89 (26%), Positives = 44/89 (48%), Gaps = 2/89 (2%)
Frame = +1
Query: 7 KLVSFWASSYGKKVEWALKLKGVE--YEYVEEDIFNKSNLLLELNPVHKKVPVLVHGNKP 64
+L + + Y ++V KG++ + V D+ N+ E KVP L H K
Sbjct: 118 RLYTNYNCPYAQRVWITRNYKGLQDKIQLVPIDLQNRPAWYKEKVYPENKVPSLEHNGKV 297
Query: 65 IAESLIILEYIDETWKHQYPLFPQDPYER 93
+ ESL +++Y+D ++ +FP DP ++
Sbjct: 298 LGESLDLIKYVDANFEGP-SIFPNDPSKK 381
>AW163994
Length = 392
Score = 32.7 bits (73), Expect = 0.050
Identities = 21/61 (34%), Positives = 30/61 (48%)
Frame = +3
Query: 30 EYEYVEEDIFNKSNLLLELNPVHKKVPVLVHGNKPIAESLIILEYIDETWKHQYPLFPQD 89
E + V D+ N+ E KVP L H K + ESL +++YID ++ L P D
Sbjct: 207 EVKLVPLDLSNRPAWYKEKVYPENKVPSLEHNGKVLGESLDLIKYIDANFEGP-SLVPTD 383
Query: 90 P 90
P
Sbjct: 384 P 386
>TC10184 similar to UP|DCAM_PHANI (Q96471) S-adenosylmethionine
decarboxylase proenzyme (AdoMetDC) (SamDC) [Contains:
S-adenosylmethionine decarboxylase alpha chain;
S-adenosylmethionine decarboxylase beta chain] , partial
(47%)
Length = 991
Score = 28.1 bits (61), Expect = 1.2
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = -1
Query: 42 SNLLLELNPVHKKVPVLVHGNKPIAESLIILEYIDETW 79
S+L L+ H K PV++ +K A+ ++L Y D+ W
Sbjct: 172 SSLHLQTPLAHSKPPVIIENSKN-ADQCMVLRYHDQAW 62
>AV421359
Length = 225
Score = 27.7 bits (60), Expect = 1.6
Identities = 17/50 (34%), Positives = 22/50 (44%)
Frame = +1
Query: 122 QENAMKEATEGLEMVEEQIKGKKFFGGITLATLTLQLVGSLTGFLFGRKL 171
Q K T + + G KF GG+ LAT L GSL F ++L
Sbjct: 37 QSTGPKTHTTSPAVTGTSVVGVKFNGGVALATDNLASYGSLARFTDTKRL 186
>TC10843
Length = 600
Score = 25.8 bits (55), Expect = 6.2
Identities = 20/85 (23%), Positives = 36/85 (41%), Gaps = 5/85 (5%)
Frame = -3
Query: 84 PLFPQDPYERALARFWAASIEPKLLKAAWVELFTSGDEQENAMKEATEGLEMVEEQ---- 139
PL+P D + F+ + +PK+ W E + + +GL +++++
Sbjct: 460 PLYPHDASPSS*EHFYQVAYDPKI--CLWT------GEASKLLTTSNQGLSIIKKELFLI 305
Query: 140 -IKGKKFFGGITLATLTLQLVGSLT 163
+K K F T + L LV LT
Sbjct: 304 WVKNKTGFSNGTTSKFFLYLVLFLT 230
>TC13762 similar to UP|Q9LDE2 (Q9LDE2) F10B6.5 (T5E21.17)
(At1g14680/F10B6_22), partial (42%)
Length = 568
Score = 25.4 bits (54), Expect = 8.0
Identities = 13/22 (59%), Positives = 14/22 (63%)
Frame = -1
Query: 145 FFGGITLATLTLQLVGSLTGFL 166
FFGG+T T TL L L GFL
Sbjct: 316 FFGGLTR*TATLLLSFGLRGFL 251
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.328 0.144 0.443
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,739,960
Number of Sequences: 28460
Number of extensions: 48610
Number of successful extensions: 270
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 263
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 263
length of query: 220
length of database: 4,897,600
effective HSP length: 87
effective length of query: 133
effective length of database: 2,421,580
effective search space: 322070140
effective search space used: 322070140
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 53 (25.0 bits)
Lotus: description of TM0162b.5