BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0154.9
(839 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18710 125 3e-29
AV767121 97 1e-20
TC18198 weakly similar to UP|Q9XGZ5 (Q9XGZ5) T1N24.20 protein, p... 88 5e-18
AV780221 62 3e-10
CB828232 57 9e-09
TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%) 49 3e-06
TC19704 weakly similar to UP|Q9SZ87 (Q9SZ87) RNA-directed DNA po... 47 2e-05
BP083121 34 0.013
BP085058 34 0.11
TC12829 30 1.2
AV426437 30 1.2
TC18895 28 4.6
BP060613 28 4.6
TC8141 weakly similar to UP|C79B_ARATH (O81346) Cytochrome P450 ... 28 6.0
TC17916 weakly similar to PIR|B97853|B97853 NADH2 dehydrogenase ... 28 6.0
>TC18710
Length = 843
Score = 125 bits (314), Expect = 3e-29
Identities = 72/228 (31%), Positives = 113/228 (48%), Gaps = 3/228 (1%)
Frame = +2
Query: 92 EEIRTAVWDCEGDKSPGPDGYNFHFIKSFWHVLKDDIVRVLEDFHTNGVWPKEGNSSFIT 151
+++R + E + G DG N F + V+KD + + F P E N + +T
Sbjct: 140 KKLRLLFFLLETSQPRGMDGMNGLFYQKNGDVVKDSVT*AILAFLERAEIPNEINETLVT 319
Query: 152 LIPKVNNPMGLNDYIPISLIGCMYKIVSKVLTTRLSKVMGHIIDENQSAFLEGRQLLDSV 211
LIPKV +P +N + PIS +YK++SK+ RL M +I QS F++GRQ+ D++
Sbjct: 320 LIPKVPHPESINQFRPISGCTFLYKVISKIFVARLKDAMPDLISPMQSGFIQGRQIQDNL 499
Query: 212 LVANE---VIDEAKVKKRGFLVFKVDYEKVYDSVNWNFLFYMLRRLGFYAKWIGWIKGCI 268
L+ E I+ R + K+D K YD V W FL L GF W+ I +
Sbjct: 500 LIVQEAFHAINRPGALGRNHSIIKLDMNKAYDRVEWKFLESSLLAFGFSTNWVKMIMILV 679
Query: 269 QSASVSVLVNGSPTDEF*MEKRLMLGDPLAPFLFLIVAECLSGMMRQA 316
S + +NG + ++ L GDP +P+LFL E LS +++ +
Sbjct: 680 SGVSYNYKINGVVGPKLLPQRGLRQGDPFSPYLFLFTMEVLSLLIQNS 823
>AV767121
Length = 525
Score = 96.7 bits (239), Expect = 1e-20
Identities = 43/71 (60%), Positives = 56/71 (78%)
Frame = +2
Query: 433 LSLWKRKTLSFGGKVCLIRSVLSSIPLFYLSFFKLPKGVASLCNRIEKQFLWGGEEGTRK 492
LS WK+KT+SFGG++ LI+SVL+++PLF+LSFFKLP GV C R+ + FLWGG E K
Sbjct: 2 LSKWKQKTVSFGGRIFLIQSVLTALPLFFLSFFKLPIGVGKSCVRLMRNFLWGGSENENK 181
Query: 493 IAWVKWSKVCR 503
IA VKW+ VC+
Sbjct: 182 IA*VKWTDVCK 214
>TC18198 weakly similar to UP|Q9XGZ5 (Q9XGZ5) T1N24.20 protein, partial
(14%)
Length = 592
Score = 88.2 bits (217), Expect = 5e-18
Identities = 61/190 (32%), Positives = 96/190 (50%), Gaps = 3/190 (1%)
Frame = -3
Query: 8 VKDGDSNTKFFHNSINWRRRTNAIRGLV-VDGGWVEDPKVVKSKMKEFFEARFTNISGDG 66
+K GD NT+FFH S RR N I + G WVE K V +F +++ S
Sbjct: 578 LK*GDHNTRFFHASTIQRRDFNRILKIKDAHGIWVEGQKKVNEAAVNYFSDIYSS-SPLQ 402
Query: 67 VLLE--GTTFRSVSEEDNLALTRTFNLEEIRTAVWDCEGDKSPGPDGYNFHFIKSFWHVL 124
L E + V++ N L + +EI+ AV+ K+PG DG N F + ++
Sbjct: 401 YLRECLAPIPKLVTDRLNHKLCAQVSTDEIKEAVFSMGDLKAPGMDGINGLFYQQNREIV 222
Query: 125 KDDIVRVLEDFHTNGVWPKEGNSSFITLIPKVNNPMGLNDYIPISLIGCMYKIVSKVLTT 184
K+D+ + DF +G P E N + +TLIPK+ + + + PIS +YK++SK++
Sbjct: 221 KNDVNNAILDFFDHGELPVELNETLVTLIPKIPHAEAIQQFRPISCCSFIYKVISKIIVA 42
Query: 185 RLSKVMGHII 194
RL M ++I
Sbjct: 41 RLKPDMVNLI 12
>AV780221
Length = 461
Score = 62.4 bits (150), Expect = 3e-10
Identities = 34/63 (53%), Positives = 41/63 (64%), Gaps = 1/63 (1%)
Frame = +3
Query: 447 VCLIRSVLSSIPLFYLSFFKLPK-GVASLCNRIEKQFLWGGEEGTRKIAWVKWSKVCRLR 505
+CLIRSVL ++PLF+L FFKLP G + N F EG +KIAWVKWS VCR +
Sbjct: 3 LCLIRSVLIALPLFHLPFFKLPTGGFYTNVNN**DPFFGREVEGGKKIAWVKWSTVCRPK 182
Query: 506 MEG 508
EG
Sbjct: 183 DEG 191
>CB828232
Length = 532
Score = 57.4 bits (137), Expect = 9e-09
Identities = 28/50 (56%), Positives = 37/50 (74%)
Frame = +1
Query: 436 WKRKTLSFGGKVCLIRSVLSSIPLFYLSFFKLPKGVASLCNRIEKQFLWG 485
WK LS G +VC + VLSS+PLF+LSFFK+ K VA +C +I+ +FLWG
Sbjct: 388 WKTLCLS-GARVCCFKMVLSSLPLFFLSFFKI-KCVAKVCKQIQSRFLWG 531
>TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%)
Length = 912
Score = 48.9 bits (115), Expect = 3e-06
Identities = 34/125 (27%), Positives = 63/125 (50%)
Frame = -3
Query: 333 EVSLLQFADDTLFIYEPSTHNVLAMKSMLRCFELMSGLKVNFFKSKLAGVSVAEDVLLRY 392
E+S L FAD+ + ++ + ++L F+ +SGL N KS++ + V E +
Sbjct: 376 EISHLCFADNLMVFSNGYLESIAIINNVLHIFQHLSGLTPNPAKSEVF-ILVKETTKNQI 200
Query: 393 ANLLHCKTTEIPFVYLGIHVGANPRKKTTWDPLLSKLRKRLSLWKRKTLSFGGKVCLIRS 452
N+L K ++P LG+ K + L+ + +L W ++LS+ G++ LI S
Sbjct: 199 TNMLGYKEGKLPVR*LGVPQLTTKLKASDCKVLVDRKPSKLQHWTGRSLSYAGRLQLINS 20
Query: 453 VLSSI 457
+L S+
Sbjct: 19 ILFSM 5
>TC19704 weakly similar to UP|Q9SZ87 (Q9SZ87) RNA-directed DNA
polymerase-like protein, partial (3%)
Length = 530
Score = 46.6 bits (109), Expect = 2e-05
Identities = 22/56 (39%), Positives = 31/56 (55%), Gaps = 1/56 (1%)
Frame = -3
Query: 1 QKSRDRWVKDGDSNTKFFHNSINWRRRTNAIRGLV-VDGGWVEDPKVVKSKMKEFF 55
Q+SR +W+K GD NTKFFH S RR N I + + G WV + + +F+
Sbjct: 252 QRSRIKWLKSGDKNTKFFHASTVQRRERNRIERIKDMQGTWVRSQLAINRDVPDFY 85
>BP083121
Length = 527
Score = 34.3 bits (77), Expect(2) = 0.013
Identities = 21/58 (36%), Positives = 32/58 (54%)
Frame = +3
Query: 299 PFLFLIVAECLSGMMRQARRLNLYKGFKVGREGVEVSLLQFADDTLFIYEPSTHNVLA 356
P FLIVAE L+G+++Q +N +KGF G ++ AD F + S N+L+
Sbjct: 312 PSFFLIVAEGLNGLLKQVATINKFKGFTFHLRGFPSK*VK-ADLHFFTFFESFPNLLS 482
Score = 21.6 bits (44), Expect(2) = 0.013
Identities = 11/19 (57%), Positives = 12/19 (62%)
Frame = +2
Query: 284 EF*MEKRLMLGDPLAPFLF 302
EF M K L DP+A FLF
Sbjct: 266 EFKMWKSLHQVDPMAAFLF 322
>BP085058
Length = 452
Score = 33.9 bits (76), Expect = 0.11
Identities = 17/70 (24%), Positives = 34/70 (48%)
Frame = +2
Query: 426 LSKLRKRLSLWKRKTLSFGGKVCLIRSVLSSIPLFYLSFFKLPKGVASLCNRIEKQFLWG 485
L ++ RL+ K L+ ++ L + VLS+I ++ + LP+ + + + F+W
Sbjct: 194 LDRVISRLAC*KAYLLNKPARLTLAKPVLSAISMYVMQLNWLPQSICDQICTVVRHFVWE 373
Query: 486 GEEGTRKIAW 495
G +AW
Sbjct: 374 NVNGIPLVAW 403
>TC12829
Length = 448
Score = 30.4 bits (67), Expect = 1.2
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = +1
Query: 482 FLWGGEEGTRKIAWVKWSKVC 502
F+W G+ R I W+ W K+C
Sbjct: 385 FIWSGDVSRRSIHWLGWKKLC 447
>AV426437
Length = 303
Score = 30.4 bits (67), Expect = 1.2
Identities = 13/42 (30%), Positives = 18/42 (41%)
Frame = +1
Query: 487 EEGTRKIAWVKWSKVCRLRMEGSWESRT*SCLFLPCWLNGIG 528
+E RKIAW+ W +C+ P WL G+G
Sbjct: 211 DENQRKIAWINWETICK-----------------PKWLRGLG 285
>TC18895
Length = 416
Score = 28.5 bits (62), Expect = 4.6
Identities = 17/37 (45%), Positives = 23/37 (61%)
Frame = +2
Query: 447 VCLIRSVLSSIPLFYLSFFKLPKGVASLCNRIEKQFL 483
VCLIR++LSS+ +S LPK + SLC+ K L
Sbjct: 287 VCLIRNILSSV----ISVCCLPKLLESLCDLNSKSTL 385
>BP060613
Length = 378
Score = 28.5 bits (62), Expect = 4.6
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = +1
Query: 483 LWGGEEGTRKIAWVKWSKVCRLRMEG 508
+W +GTR + W KW + L+ EG
Sbjct: 43 VWWSSKGTRGVHWRKWDLLTELKDEG 120
>TC8141 weakly similar to UP|C79B_ARATH (O81346) Cytochrome P450 79B2 ,
partial (60%)
Length = 1919
Score = 28.1 bits (61), Expect = 6.0
Identities = 23/81 (28%), Positives = 38/81 (46%), Gaps = 8/81 (9%)
Frame = -3
Query: 342 DTLFIY---EP-STHNVLAMKSMLRCFELMSGLKVN----FFKSKLAGVSVAEDVLLRYA 393
+TLFI+ P T V+ S+ +++ GL FFK+ A V + + +A
Sbjct: 468 NTLFIFFHCSP*GTRTVVMKPSLAISVDMIFGLDAKEASCFFKNSQAIVGHVTGITMVFA 289
Query: 394 NLLHCKTTEIPFVYLGIHVGA 414
+H + I F+ L IH+ A
Sbjct: 288 RRIHAISVFISFISLCIHIVA 226
>TC17916 weakly similar to PIR|B97853|B97853 NADH2 dehydrogenase
(ubiquinone) - Rickettsia conorii
(strain Malish 7) {Rickettsia conorii;} , partial (3%)
Length = 520
Score = 28.1 bits (61), Expect = 6.0
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = -2
Query: 460 FYLSFFKLPKGVASLCNRIEKQFLWGGEEGTRKIAWV 496
F+LSF L GV + K +WG E+ + AW+
Sbjct: 357 FFLSFKFLLSGVEITLVSLIKTSIWGKEKNEEQFAWI 247
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.337 0.148 0.491
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,433,557
Number of Sequences: 28460
Number of extensions: 233236
Number of successful extensions: 1676
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 1655
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1672
length of query: 839
length of database: 4,897,600
effective HSP length: 98
effective length of query: 741
effective length of database: 2,108,520
effective search space: 1562413320
effective search space used: 1562413320
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.7 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0154.9