BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0154.7
(495 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV408392 236 6e-63
TC19615 similar to UP|IF39_TOBAC (P56821) Eukaryotic translation... 166 7e-45
BP053577 50 1e-06
TC15356 weakly similar to UP|Q7QCA3 (Q7QCA3) AgCP1462 (Fragment)... 44 5e-05
BP032481 44 8e-05
BP080049 42 2e-04
AV779667 39 0.002
TC16779 similar to UP|Q9LEY9 (Q9LEY9) Nhp2-like protein (AT5g081... 35 0.037
BP069359 35 0.037
AV776853 33 0.14
AV426581 33 0.14
TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, ... 32 0.31
TC20198 similar to UP|Q9ZPU0 (Q9ZPU0) At2g13980 protein, partial... 31 0.53
CN825379 31 0.53
TC19735 similar to UP|Q8NJM5 (Q8NJM5) Probable ATP-dependent RNA... 30 0.69
TC16026 similar to UP|Q9SGZ3 (Q9SGZ3) F28K19.29, partial (32%) 30 0.69
BP031757 30 0.90
BP034655 30 0.90
TC8358 similar to UP|Q84T14 (Q84T14) Vacuolar ATPase subunit E (... 30 1.2
TC14320 similar to UP|Q41111 (Q41111) Dehydrin, partial (56%) 30 1.2
>AV408392
Length = 425
Score = 236 bits (602), Expect = 6e-63
Identities = 114/129 (88%), Positives = 122/129 (94%)
Frame = +3
Query: 205 LNRHTKTKKSTYTGFELFRIKERDIPIEVLELENKNDKIIAFAWEPKGHRFVVIHGDDSK 264
++R+TKTKKSTYTGFELFRIKERDIPIEVLELENKNDKIIAFAWEPKGHRFVVIHG++ K
Sbjct: 39 VDRYTKTKKSTYTGFELFRIKERDIPIEVLELENKNDKIIAFAWEPKGHRFVVIHGENPK 218
Query: 265 PDVSIYSMRTAQKAGWVSKLTALKGKQANALFWSPAGRFIVVAGLKGFNGQLEFYNVDEL 324
PD+SIYSMRTAQ G VSKLT LKGKQANALFWSPAGRFIV+AGLKG NGQLEFYNVDEL
Sbjct: 219 PDISIYSMRTAQHPGRVSKLTTLKGKQANALFWSPAGRFIVLAGLKGLNGQLEFYNVDEL 398
Query: 325 ETMVTAEHF 333
ETM TA+HF
Sbjct: 399 ETMATADHF 425
>TC19615 similar to UP|IF39_TOBAC (P56821) Eukaryotic translation initiation
factor 3 subunit 9 (eIF-3 eta) (eIF3 p110) (eIF3b),
partial (14%)
Length = 553
Score = 166 bits (419), Expect(3) = 7e-45
Identities = 77/94 (81%), Positives = 85/94 (89%)
Frame = +3
Query: 372 YRIMKDHFFQFSWRARPPSFLTPEKEEEIANNLKKYSKKYEAEDQDESMLLSEQEQEKCR 431
YRI+KDHFFQF WR RPPSFLTPE+EEEIA +LKKYSKKYEAEDQD S+LLSEQE+EK R
Sbjct: 3 YRILKDHFFQFLWRPRPPSFLTPEQEEEIAKSLKKYSKKYEAEDQDVSLLLSEQEREKRR 182
Query: 432 MLKEEWDMWVNEWKWMHEEEKLQREQHRDGETSD 465
MLKE+WD WV+EWK MHEEEKLQRE+ RDGE SD
Sbjct: 183 MLKEDWDKWVSEWKRMHEEEKLQREELRDGEASD 284
Score = 28.5 bits (62), Expect(3) = 7e-45
Identities = 14/21 (66%), Positives = 16/21 (75%)
Frame = +2
Query: 475 IEVEEVIDVSEEILHFEYGQE 495
+ VEEVIDV EE+L FE G E
Sbjct: 314 LNVEEVIDVCEEVLPFE*G*E 376
Score = 24.3 bits (51), Expect(3) = 7e-45
Identities = 9/11 (81%), Positives = 11/11 (99%)
Frame = +1
Query: 466 EEEEYEAKDIE 476
EEEEYEA+D+E
Sbjct: 286 EEEEYEARDLE 318
>BP053577
Length = 463
Score = 49.7 bits (117), Expect = 1e-06
Identities = 22/42 (52%), Positives = 32/42 (75%)
Frame = +3
Query: 86 LSFVRRSGNSVADFLAKNSSKFPNVVWLEEILPKFDLLVSSD 127
+SFVRR+GN+VADFLA+NS + N+VW+EE+ L+ +D
Sbjct: 288 VSFVRRTGNTVADFLARNSDTYANMVWVEEVPLTVVSLIDAD 413
>TC15356 weakly similar to UP|Q7QCA3 (Q7QCA3) AgCP1462 (Fragment), partial
(5%)
Length = 806
Score = 44.3 bits (103), Expect = 5e-05
Identities = 21/78 (26%), Positives = 38/78 (47%), Gaps = 1/78 (1%)
Frame = +2
Query: 314 GQLEFYNVDELETMVTAEHFMTTDIEWDPTGRYVTTSVTSVH-DVENGFNIWSFSGKHLY 372
G + F++ + + + + + EW P G Y T+ T+ V+NG I+ ++G +
Sbjct: 47 GDMVFWDYADKKQLAATKAEWSVTSEWSPDGCYFMTATTAPRLQVDNGIKIFHYNGSLYF 226
Query: 373 RIMKDHFFQFSWRARPPS 390
+ M D FQ W+ PS
Sbjct: 227 KKMFDKLFQADWKPESPS 280
>BP032481
Length = 447
Score = 43.5 bits (101), Expect = 8e-05
Identities = 18/31 (58%), Positives = 25/31 (80%)
Frame = -1
Query: 86 LSFVRRSGNSVADFLAKNSSKFPNVVWLEEI 116
LSFVRR+GN+VA F A+N+ + N+VW EE+
Sbjct: 159 LSFVRRTGNTVAGFFARNAETYANLVWPEEV 67
>BP080049
Length = 356
Score = 42.4 bits (98), Expect = 2e-04
Identities = 22/48 (45%), Positives = 29/48 (59%)
Frame = -1
Query: 83 IHDLSFVRRSGNSVADFLAKNSSKFPNVVWLEEILPKFDLLVSSDAFI 130
+ LSFVRR+ N V D LAK + NVVW+EE+ + LV SD +
Sbjct: 191 VFQLSFVRRTSNCVVDALAKLCFQLGNVVWVEELPHELYGLVQSDVMV 48
>AV779667
Length = 455
Score = 38.9 bits (89), Expect = 0.002
Identities = 18/41 (43%), Positives = 26/41 (62%)
Frame = -3
Query: 87 SFVRRSGNSVADFLAKNSSKFPNVVWLEEILPKFDLLVSSD 127
S + R GNS AD+LA+N+S +P+ VW+ E P L+ D
Sbjct: 171 SHMGREGNSCADYLARNASSYPDSVWI*EGPPGLSSLLRDD 49
>TC16779 similar to UP|Q9LEY9 (Q9LEY9) Nhp2-like protein
(AT5g08180/T22D6_120), partial (17%)
Length = 850
Score = 34.7 bits (78), Expect = 0.037
Identities = 13/19 (68%), Positives = 18/19 (94%)
Frame = +3
Query: 345 RYVTTSVTSVHDVENGFNI 363
+YV T+VTSVH++ENGFN+
Sbjct: 171 KYVATAVTSVHEMENGFNL 227
>BP069359
Length = 510
Score = 34.7 bits (78), Expect = 0.037
Identities = 15/22 (68%), Positives = 18/22 (81%)
Frame = -1
Query: 82 LIHDLSFVRRSGNSVADFLAKN 103
+++ LSFVRRSGN VADFL N
Sbjct: 69 MLYSLSFVRRSGNCVADFLTMN 4
>AV776853
Length = 625
Score = 32.7 bits (73), Expect = 0.14
Identities = 14/40 (35%), Positives = 25/40 (62%), Gaps = 1/40 (2%)
Frame = +1
Query: 457 QHRDGETSDEEEEYEAKDIEVEEVIDVS-EEILHFEYGQE 495
+H D DE E+ +AKD + ++D+S ++I H ++G E
Sbjct: 136 EHNDLGDKDEAEDVDAKDDHYKRILDLSADDIRHLDFGSE 255
>AV426581
Length = 429
Score = 32.7 bits (73), Expect = 0.14
Identities = 21/62 (33%), Positives = 36/62 (57%), Gaps = 5/62 (8%)
Frame = -2
Query: 429 KCRMLKEEWDMWVNEWKWMHEEEKLQREQHRDGETSDEEEEYEAKDIEV-----EEVIDV 483
+CR + +W +W +WKW K +R++ + +T +EEEE E I++ +EV D+
Sbjct: 242 RCRRRRGKW-IW--KWKW-----KWKRKRDSNLKTVEEEEEEEEDPIKLYKKMQKEVCDI 87
Query: 484 SE 485
SE
Sbjct: 86 SE 81
>TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, partial
(31%)
Length = 727
Score = 31.6 bits (70), Expect = 0.31
Identities = 26/90 (28%), Positives = 37/90 (40%)
Frame = +1
Query: 406 KYSKKYEAEDQDESMLLSEQEQEKCRMLKEEWDMWVNEWKWMHEEEKLQREQHRDGETSD 465
K K EAE Q+ + E E+E + EEE+ + E+ E +
Sbjct: 160 KLQNKLEAEVQEYEEVEEEVEEE------------------VEEEEEEEEEEEEGEEEEE 285
Query: 466 EEEEYEAKDIEVEEVIDVSEEILHFEYGQE 495
EEEE E + E EE D + L G+E
Sbjct: 286 EEEEEEEVEAEAEEEDDEPIQKLLEPMGKE 375
Score = 31.2 bits (69), Expect = 0.41
Identities = 25/93 (26%), Positives = 41/93 (43%)
Frame = +1
Query: 395 EKEEEIANNLKKYSKKYEAEDQDESMLLSEQEQEKCRMLKEEWDMWVNEWKWMHEEEKLQ 454
E E N K+ +S L + Q K +E++ E + EEE+ +
Sbjct: 70 ESPNEAMVNESITKKRKLVPKSSQSAELPFKLQNKLEAEVQEYEEVEEEVEEEVEEEEEE 249
Query: 455 REQHRDGETSDEEEEYEAKDIEVEEVIDVSEEI 487
E+ +GE +EEEE E +++E E + E I
Sbjct: 250 EEEEEEGEEEEEEEE-EEEEVEAEAEEEDDEPI 345
>TC20198 similar to UP|Q9ZPU0 (Q9ZPU0) At2g13980 protein, partial (13%)
Length = 630
Score = 30.8 bits (68), Expect = 0.53
Identities = 17/31 (54%), Positives = 21/31 (66%)
Frame = +3
Query: 85 DLSFVRRSGNSVADFLAKNSSKFPNVVWLEE 115
DLSFVRR GN VA+ LAK + VW+E+
Sbjct: 417 DLSFVRR-GNKVANDLAKLAFSSHGYVWIED 506
>CN825379
Length = 646
Score = 30.8 bits (68), Expect = 0.53
Identities = 24/93 (25%), Positives = 50/93 (52%), Gaps = 2/93 (2%)
Frame = +3
Query: 397 EEEIANNLKKYSKKYEAEDQ--DESMLLSEQEQEKCRMLKEEWDMWVNEWKWMHEEEKLQ 454
E+ + L + + + ED+ + L ++Q++++ RM +E++ E + EE++ +
Sbjct: 108 EKMVTRRLAQIATSDDDEDEAPKQESLEAKQQRKRKRMKLQEYEDEEAEVV-VEEEDEEK 284
Query: 455 REQHRDGETSDEEEEYEAKDIEVEEVIDVSEEI 487
++ R+ TSD EEE +D +E+ V E I
Sbjct: 285 KKNQRNPPTSDAEEE---QDAPLEDAKPVGESI 374
>TC19735 similar to UP|Q8NJM5 (Q8NJM5) Probable ATP-dependent RNA helicase,
partial (5%)
Length = 565
Score = 30.4 bits (67), Expect = 0.69
Identities = 25/94 (26%), Positives = 41/94 (43%)
Frame = +3
Query: 386 ARPPSFLTPEKEEEIANNLKKYSKKYEAEDQDESMLLSEQEQEKCRMLKEEWDMWVNEWK 445
A P L E NN KK KK + + + + +E+ E+
Sbjct: 120 AEPEDNLQSSPMVETENNKKKKEKKNKHDKKRPRDVANEEHAEE---------------- 251
Query: 446 WMHEEEKLQREQHRDGETSDEEEEYEAKDIEVEE 479
EEE++ RDGE+S ++++ + K +EVEE
Sbjct: 252 ---EEEQIGNNSDRDGESSKKKKKKKHK-VEVEE 341
>TC16026 similar to UP|Q9SGZ3 (Q9SGZ3) F28K19.29, partial (32%)
Length = 1183
Score = 30.4 bits (67), Expect = 0.69
Identities = 25/66 (37%), Positives = 35/66 (52%), Gaps = 1/66 (1%)
Frame = -2
Query: 424 EQEQEKCRMLKEEWDMWVNEWKWMHEEEKLQREQHRDGETSDEEEEYEAKDIE-VEEVID 482
EQ EK + + E E K + EE+ + E DGE +EEEE E ++ E VE +
Sbjct: 774 EQPSEKKGVTEGE------E*KELGGEEQGRDEFDEDGEGEEEEEEEEEEEGEKVEVEVG 613
Query: 483 VSEEIL 488
V EE+L
Sbjct: 612 VGEELL 595
>BP031757
Length = 507
Score = 30.0 bits (66), Expect = 0.90
Identities = 17/76 (22%), Positives = 41/76 (53%)
Frame = +2
Query: 396 KEEEIANNLKKYSKKYEAEDQDESMLLSEQEQEKCRMLKEEWDMWVNEWKWMHEEEKLQR 455
KE+ + + + + E++ L +++++ ++L+E+ + E + EEEK ++
Sbjct: 50 KEKMVNRRFTQVATSDDEEEETPPQQLKPRKRKRMKLLEEDNEDKDGE-DYEEEEEKKKK 226
Query: 456 EQHRDGETSDEEEEYE 471
++ R + +EEEE E
Sbjct: 227 KKSRKKKEEEEEEEQE 274
>BP034655
Length = 517
Score = 30.0 bits (66), Expect = 0.90
Identities = 26/102 (25%), Positives = 44/102 (42%), Gaps = 9/102 (8%)
Frame = +1
Query: 378 HFFQF--SWRARPPSFLTP-------EKEEEIANNLKKYSKKYEAEDQDESMLLSEQEQE 428
HF F +++ PP F TP + E A +L +D++E E+E+E
Sbjct: 4 HFLYFLAAFKRPPPLFATPMASPSEFHRNEIAAAHLTAADFGLSDDDEEEEEEEEEEEEE 183
Query: 429 KCRMLKEEWDMWVNEWKWMHEEEKLQREQHRDGETSDEEEEY 470
+ EE EEE+ + E+ + + DE+E+Y
Sbjct: 184 E-----EE------------EEEEEEEEEDEEDDEEDEDEDY 258
Score = 28.9 bits (63), Expect = 2.0
Identities = 13/31 (41%), Positives = 20/31 (63%)
Frame = +1
Query: 449 EEEKLQREQHRDGETSDEEEEYEAKDIEVEE 479
EEE+ + E+ + E +EEEE E +D E +E
Sbjct: 145 EEEEEEEEEEEEEEEEEEEEEEEEEDEEDDE 237
Score = 28.1 bits (61), Expect = 3.4
Identities = 12/31 (38%), Positives = 20/31 (63%)
Frame = +1
Query: 449 EEEKLQREQHRDGETSDEEEEYEAKDIEVEE 479
EEE+ + E+ + E +EEEE + +D E +E
Sbjct: 154 EEEEEEEEEEEEEEEEEEEEEEDEEDDEEDE 246
Score = 27.7 bits (60), Expect = 4.5
Identities = 13/31 (41%), Positives = 19/31 (60%)
Frame = +1
Query: 449 EEEKLQREQHRDGETSDEEEEYEAKDIEVEE 479
EEE+ + E+ + E +EEEE E D E E+
Sbjct: 157 EEEEEEEEEEEEEEEEEEEEEDEEDDEEDED 249
Score = 26.6 bits (57), Expect = 10.0
Identities = 12/31 (38%), Positives = 19/31 (60%)
Frame = +1
Query: 449 EEEKLQREQHRDGETSDEEEEYEAKDIEVEE 479
EEE+ + E+ + E +EEEE E + + EE
Sbjct: 148 EEEEEEEEEEEEEEEEEEEEEEEEDEEDDEE 240
>TC8358 similar to UP|Q84T14 (Q84T14) Vacuolar ATPase subunit E (Fragment),
complete
Length = 1321
Score = 29.6 bits (65), Expect = 1.2
Identities = 13/47 (27%), Positives = 28/47 (58%)
Frame = +3
Query: 81 YLIHDLSFVRRSGNSVADFLAKNSSKFPNVVWLEEILPKFDLLVSSD 127
++ LS++ ++GN ADF+ + + N VW+E+ + L+++D
Sbjct: 1134 FISFHLSYICQNGNKAADFM*RLAYSHNNSVWIEDDHVGLEPLLAND 1274
>TC14320 similar to UP|Q41111 (Q41111) Dehydrin, partial (56%)
Length = 1231
Score = 29.6 bits (65), Expect = 1.2
Identities = 27/88 (30%), Positives = 39/88 (43%), Gaps = 6/88 (6%)
Frame = +3
Query: 393 TPEKEEEIANNLKKYSKKYEAEDQDESMLLSEQEQ----EKCRMLKEEWDMWVNEWKWMH 448
T E E + KK E E E ++++E E+ E+ KEE D +E + H
Sbjct: 393 TTEVETQDRGVFDFLGKKKEEEKPQEEVIVTEFEKVKVSEEPEKKKEEVD---HEGEKKH 563
Query: 449 EE--EKLQREQHRDGETSDEEEEYEAKD 474
EKL R +SDEEE E ++
Sbjct: 564 STLLEKLHRSDSSSSSSSDEEEGKEGEE 647
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.135 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,433,792
Number of Sequences: 28460
Number of extensions: 107939
Number of successful extensions: 780
Number of sequences better than 10.0: 63
Number of HSP's better than 10.0 without gapping: 723
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 759
length of query: 495
length of database: 4,897,600
effective HSP length: 94
effective length of query: 401
effective length of database: 2,222,360
effective search space: 891166360
effective search space used: 891166360
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0154.7