BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0150.12
(266 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC11548 similar to UP|Q9FG37 (Q9FG37) Gamma-tubulin interacting ... 36 0.008
AV409241 34 0.022
AV423678 32 0.085
TC18562 homologue to UP|Q9FVA6 (Q9FVA6) Glu-tRNA(Gln) amidotrans... 32 0.085
TC9650 similar to UP|O49485 (O49485) Phosphoglycerate dehydrogen... 32 0.11
TC7796 homologue to gb|AF036495.1|AF036495 Hamamelis virginiana ... 32 0.11
TC8131 similar to UP|Q9XFM6 (Q9XFM6) Progesterone-binding protei... 32 0.15
TC8484 similar to UP|Q9XGP1 (Q9XGP1) ESTs AU070372(S13446), part... 32 0.15
TC16186 weakly similar to UP|Q9LTB9 (Q9LTB9) Genomic DNA, chromo... 31 0.19
AV769326 31 0.25
TC14852 similar to UP|Q9LZI2 (Q9LZI2) DTDP-glucose 4-6-dehydrata... 31 0.25
AV410706 30 0.32
TC15357 similar to UP|Q9ZV06 (Q9ZV06) At2g39020 protein, partial... 30 0.32
TC9027 weakly similar to UP|AAQ82447 (AAQ82447) SufB, partial (51%) 30 0.32
TC16458 similar to UP|O48679 (O48679) F3I6.5 protein, partial (20%) 30 0.32
TC14816 30 0.42
TC12323 30 0.42
TC15655 similar to UP|Q8LE99 (Q8LE99) Transketolase-like protein... 30 0.42
AV408621 30 0.55
BP063442 30 0.55
>TC11548 similar to UP|Q9FG37 (Q9FG37) Gamma-tubulin interacting
protein-like, partial (6%)
Length = 480
Score = 35.8 bits (81), Expect = 0.008
Identities = 28/76 (36%), Positives = 38/76 (49%), Gaps = 3/76 (3%)
Frame = +3
Query: 41 KNNINDPLALSLSVEPVHT-TPTYMNPFHVSLSPPSPPSTFEPPLASTND--SIDSSSSR 97
K+N PL + P H+ +PTY +P SPP EPPL +TN S S SS+
Sbjct: 204 KSNAVSPLT---AAPPTHSRSPTY--------TPSSPP---EPPLLTTNGPCSTSSRSSQ 341
Query: 98 RVRRRVEKSEIIPPPF 113
++ R + IPP F
Sbjct: 342 KIARHWLRPSSIPPSF 389
>AV409241
Length = 282
Score = 34.3 bits (77), Expect = 0.022
Identities = 20/66 (30%), Positives = 28/66 (42%), Gaps = 5/66 (7%)
Frame = +2
Query: 52 LSVEPVHTTPTYMNPFHVSLSPP-----SPPSTFEPPLASTNDSIDSSSSRRVRRRVEKS 106
+ V+P +PT P PP SPP T PP A+T + + SR++
Sbjct: 65 VQVQPPSHSPTKTPPPPTPPPPPPPATVSPPPTPSPPAAATPSPLSAKPSRKIPTSTTSP 244
Query: 107 EIIPPP 112
PPP
Sbjct: 245 RSAPPP 262
>AV423678
Length = 426
Score = 32.3 bits (72), Expect = 0.085
Identities = 22/69 (31%), Positives = 30/69 (42%), Gaps = 11/69 (15%)
Frame = +3
Query: 39 NVKNNINDPLALSLSVEPVHTTPTYMNPFH----------VSLSPPSPPS-TFEPPLAST 87
+VK P S P + P ++PFH +S PPSPP T PP +S
Sbjct: 12 SVKRRRTWPFQASTVFSPSNAVPIPISPFHPPPLTTSAAAISSPPPSPPRFTAPPPSSSV 191
Query: 88 NDSIDSSSS 96
+ S+SS
Sbjct: 192SHPTSSASS 218
>TC18562 homologue to UP|Q9FVA6 (Q9FVA6) Glu-tRNA(Gln) amidotransferase
subunit A, partial (4%)
Length = 239
Score = 32.3 bits (72), Expect = 0.085
Identities = 20/39 (51%), Positives = 25/39 (63%)
Frame = +3
Query: 47 PLALSLSVEPVHTTPTYMNPFHVSLSPPSPPSTFEPPLA 85
PLA SLS H +P+++ P SLS P PPST PPL+
Sbjct: 57 PLAPSLS----HASPSFLQPQTPSLSLP-PPSTSLPPLS 158
>TC9650 similar to UP|O49485 (O49485) Phosphoglycerate dehydrogenase-like
protein , partial (47%)
Length = 1041
Score = 32.0 bits (71), Expect = 0.11
Identities = 21/67 (31%), Positives = 32/67 (47%)
Frame = +2
Query: 45 NDPLALSLSVEPVHTTPTYMNPFHVSLSPPSPPSTFEPPLASTNDSIDSSSSRRVRRRVE 104
+ PL LS + H+ P +P + + P+P S+ PP ++ N SSS R +RV
Sbjct: 62 DSPLPHFLSHQNSHSPPPSPSPSAATATVPAPSSSSSPPPSTPNP--PSSSPRSSAKRVS 235
Query: 105 KSEIIPP 111
S P
Sbjct: 236 NSSRTSP 256
>TC7796 homologue to gb|AF036495.1|AF036495 Hamamelis virginiana large
subunit 26S ribosomal RNA gene, partial sequence,
complete
Length = 6006
Score = 32.0 bits (71), Expect = 0.11
Identities = 18/57 (31%), Positives = 27/57 (46%), Gaps = 7/57 (12%)
Frame = -3
Query: 74 PSPPSTFEPPLASTNDSIDSSSSRRVRRRVEKSEIIPP-------PFPWATDRRARV 123
P P PP + ++ + ++R VRR+ SE+ PP PFP TD R+
Sbjct: 4663 PKVPFPIRPPTGTRRPALAAEAARAVRRQPTGSELGPPCPALRANPFPEVTDPFCRL 4493
Score = 25.8 bits (55), Expect(2) = 0.95
Identities = 23/69 (33%), Positives = 31/69 (44%), Gaps = 3/69 (4%)
Frame = -1
Query: 15 CSSISSSTKSHPFFLS--QMEMKKAENVKNNINDPLALSLSVEPVHTTPTYMNPFH-VSL 71
C S++KS P Q + N NN+ +SLS+ P T PT + FH V L
Sbjct: 5190 CLPPGSTSKS*P*VQKEGQRPASDSRNK*NNVKSS-GISLSLFPAPTYPTPLKSFHKVGL 5014
Query: 72 SPPSPPSTF 80
S S+F
Sbjct: 5013 ESSSTGSSF 4987
Score = 21.2 bits (43), Expect(2) = 0.95
Identities = 6/8 (75%), Positives = 7/8 (87%)
Frame = -2
Query: 108 IIPPPFPW 115
I+P PFPW
Sbjct: 4979 ILPSPFPW 4956
>TC8131 similar to UP|Q9XFM6 (Q9XFM6) Progesterone-binding protein-like,
partial (67%)
Length = 1227
Score = 31.6 bits (70), Expect = 0.15
Identities = 20/68 (29%), Positives = 28/68 (40%), Gaps = 6/68 (8%)
Frame = +2
Query: 63 YMNPFHVSLSPPSPPSTFEPPLAST------NDSIDSSSSRRVRRRVEKSEIIPPPFPWA 116
Y F SL+PP+P + EPP S+ S S R R + I PF W
Sbjct: 143 YTTSFQASLAPPTPTTAMEPPGTSSRRWSLFGRRFRSGKSPRKSLRATMALIPRSPFSWR 322
Query: 117 TDRRARVY 124
R+ ++
Sbjct: 323 LRVRSMMF 346
>TC8484 similar to UP|Q9XGP1 (Q9XGP1) ESTs AU070372(S13446), partial (76%)
Length = 1121
Score = 31.6 bits (70), Expect = 0.15
Identities = 26/79 (32%), Positives = 38/79 (47%), Gaps = 3/79 (3%)
Frame = +1
Query: 39 NVKNNINDPLALSLSVEP-VHTTPTYM-NPFHVS-LSPPSPPSTFEPPLASTNDSIDSSS 95
++ ++ +P SLS P HT ++ +P+ S L PPSPP + P +S +S S S
Sbjct: 22 SITSSTQNPFLHSLSSAPFTHTL*WHLQSPYPGSFLPPPSPPLSSHPNPSSPPNSPPSPS 201
Query: 96 SRRVRRRVEKSEIIPPPFP 114
S V PPP P
Sbjct: 202 SSTVATPNPSDSHPPPPSP 258
>TC16186 weakly similar to UP|Q9LTB9 (Q9LTB9) Genomic DNA, chromosome 5, TAC
clone:K14A3 (AT5g47090/K14A3_4), partial (54%)
Length = 549
Score = 31.2 bits (69), Expect = 0.19
Identities = 20/65 (30%), Positives = 30/65 (45%)
Frame = -3
Query: 58 HTTPTYMNPFHVSLSPPSPPSTFEPPLASTNDSIDSSSSRRVRRRVEKSEIIPPPFPWAT 117
H +PTY H + S PSP S+ PP + ++ S +S + R R + P P
Sbjct: 367 HPSPTYPGK-HAAGSSPSPWSSSAPPPSDSSRSAAASRANSPRNRPSSNRNRPAPCT*VR 191
Query: 118 DRRAR 122
+R R
Sbjct: 190 NRSVR 176
>AV769326
Length = 262
Score = 30.8 bits (68), Expect = 0.25
Identities = 19/53 (35%), Positives = 26/53 (48%)
Frame = +2
Query: 45 NDPLALSLSVEPVHTTPTYMNPFHVSLSPPSPPSTFEPPLASTNDSIDSSSSR 97
N P A S P T P+ P PS P++ EPP +S+ S S+S+R
Sbjct: 116 NPPSAASDPNSPSLTAPSLQKP-------PSSPTSTEPPSSSSTPSAPSASTR 253
>TC14852 similar to UP|Q9LZI2 (Q9LZI2) DTDP-glucose 4-6-dehydratase homolog
D18 (At3g62830/F26K9_260), partial (70%)
Length = 1470
Score = 30.8 bits (68), Expect = 0.25
Identities = 20/56 (35%), Positives = 27/56 (47%)
Frame = +1
Query: 51 SLSVEPVHTTPTYMNPFHVSLSPPSPPSTFEPPLASTNDSIDSSSSRRVRRRVEKS 106
S S P P +P + L PP PP + PPL S + S+ S++S RR S
Sbjct: 553 SSSSSPASP*PPSSSPSSLPL-PPHPPPSPPPPLMSRSRSLTSTTSTSSRRSTTAS 717
>AV410706
Length = 427
Score = 30.4 bits (67), Expect = 0.32
Identities = 18/39 (46%), Positives = 23/39 (58%)
Frame = +3
Query: 58 HTTPTYMNPFHVSLSPPSPPSTFEPPLASTNDSIDSSSS 96
HTT TY +P SPP PP +F+ L + S+ SSSS
Sbjct: 24 HTTLTYPSPLS---SPPPPPPSFKGFLFTRPISVVSSSS 131
>TC15357 similar to UP|Q9ZV06 (Q9ZV06) At2g39020 protein, partial (69%)
Length = 1578
Score = 30.4 bits (67), Expect = 0.32
Identities = 22/60 (36%), Positives = 25/60 (41%)
Frame = +1
Query: 58 HTTPTYMNPFHVSLSPPSPPSTFEPPLASTNDSIDSSSSRRVRRRVEKSEIIPPPFPWAT 117
HT N S PP+PPS L +TN S S SS KS P P P +T
Sbjct: 502 HTKWPSSNASPTSSPPPNPPSPPRSSLRTTNPSTPSPSSS------SKSPRTPSPTPTST 663
>TC9027 weakly similar to UP|AAQ82447 (AAQ82447) SufB, partial (51%)
Length = 1016
Score = 30.4 bits (67), Expect = 0.32
Identities = 20/74 (27%), Positives = 30/74 (40%), Gaps = 5/74 (6%)
Frame = +2
Query: 46 DPLALSLSVEPVHTTPTYMNPFHVSLSP-----PSPPSTFEPPLASTNDSIDSSSSRRVR 100
+PLA S S P T + P + +P P+P PP +T + S+++ R
Sbjct: 92 NPLATSTSANPTSTQTSQSPPPATTTNPSSESAPTPLPPPHPPPPTTKSARSSATATTTR 271
Query: 101 RRVEKSEIIPPPFP 114
S P P P
Sbjct: 272 NSASPSTSTPSPSP 313
>TC16458 similar to UP|O48679 (O48679) F3I6.5 protein, partial (20%)
Length = 539
Score = 30.4 bits (67), Expect = 0.32
Identities = 15/45 (33%), Positives = 26/45 (57%), Gaps = 1/45 (2%)
Frame = -3
Query: 42 NNINDPLALSLSVEPVHTTPT-YMNPFHVSLSPPSPPSTFEPPLA 85
+++ P + S +P+ +TP+ Y P H + +PP PP+T P A
Sbjct: 330 DDLPTPPHMHNSSQPLSSTPSHYYPPNH*TPNPPPPPNTISPSCA 196
>TC14816
Length = 952
Score = 30.0 bits (66), Expect = 0.42
Identities = 14/53 (26%), Positives = 27/53 (50%), Gaps = 4/53 (7%)
Frame = +2
Query: 56 PVHTTPTYMNPFHVSLS----PPSPPSTFEPPLASTNDSIDSSSSRRVRRRVE 104
PV + P N H++ S PP PP + P AS++ S+ ++ + + ++
Sbjct: 53 PVFSLPLTRNSHHIASSSPTPPPIPPQQSQSPTASSSQSVQGTAKAQPQNPIK 211
>TC12323
Length = 834
Score = 30.0 bits (66), Expect = 0.42
Identities = 15/27 (55%), Positives = 19/27 (69%), Gaps = 2/27 (7%)
Frame = +2
Query: 58 HTTP-TYMNPFH-VSLSPPSPPSTFEP 82
HT+P Y +PFH +S SPPSPP + P
Sbjct: 101 HTSPFLYPSPFHSLSSSPPSPPPRYCP 181
>TC15655 similar to UP|Q8LE99 (Q8LE99) Transketolase-like protein, partial
(17%)
Length = 575
Score = 30.0 bits (66), Expect = 0.42
Identities = 21/63 (33%), Positives = 28/63 (44%), Gaps = 11/63 (17%)
Frame = +3
Query: 51 SLSVEPVHTTPTYMNPFHVSLSPPSPPSTFEPPLASTNDSIDSSSS-----------RRV 99
SLS +P P P S SPPSP S PP S + ++ ++ RR+
Sbjct: 60 SLSPKPSSRAPLPSPPPRASPSPPSPASANPPPSPSPSPTLQPLTAAFPPPSKPPLRRRL 239
Query: 100 RRR 102
RRR
Sbjct: 240 RRR 248
>AV408621
Length = 426
Score = 29.6 bits (65), Expect = 0.55
Identities = 17/49 (34%), Positives = 22/49 (44%), Gaps = 6/49 (12%)
Frame = +1
Query: 55 EPVHTT----PTYMNPFHVSLSPPSPPST--FEPPLASTNDSIDSSSSR 97
+P+H T T+ NP H L P PP T F + N S+D R
Sbjct: 43 QPLHKTHLFIKTHQNPMHPFLHQPQPPKTDPFSSQFSVRNLSVDPEGRR 189
>BP063442
Length = 445
Score = 29.6 bits (65), Expect = 0.55
Identities = 16/61 (26%), Positives = 31/61 (50%), Gaps = 3/61 (4%)
Frame = +1
Query: 16 SSISSSTKSHPFFLSQMEMKKAENVKNNINDPLALSLS---VEPVHTTPTYMNPFHVSLS 72
S I KS+P F +++ + + +N D + + ++P +T+P NPF+ S +
Sbjct: 259 SKILFPCKSNPLFNNKIPSPATQGISSNELDEIKWDIEDEVIKPNNTSPXPSNPFNSSKT 438
Query: 73 P 73
P
Sbjct: 439 P 441
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.133 0.416
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,400,369
Number of Sequences: 28460
Number of extensions: 120712
Number of successful extensions: 1544
Number of sequences better than 10.0: 172
Number of HSP's better than 10.0 without gapping: 1381
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1499
length of query: 266
length of database: 4,897,600
effective HSP length: 89
effective length of query: 177
effective length of database: 2,364,660
effective search space: 418544820
effective search space used: 418544820
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0150.12