BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0147a.4
(354 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV427422 161 2e-40
BP055130 104 6e-25
AU089582 59 7e-13
BF177840 68 3e-12
BP054462 51 3e-12
TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase regi... 49 1e-06
BP046863 47 6e-06
CB829142 30 0.47
TC15372 similar to UP|Q8W206 (Q8W206) CSN complex subunit 6A, pa... 30 0.80
TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%) 29 1.4
TC19252 similar to UP|Q9FMS6 (Q9FMS6) CCR4-associated factor-lik... 28 1.8
TC14688 28 2.3
BP049555 28 2.3
CB828459 28 3.0
TC12227 28 3.0
BP051766 28 3.0
TC16971 weakly similar to UP|O23161 (O23161) Receptor kinase-lik... 28 3.0
AV777366 27 5.2
TC13517 similar to UP|MJDL_ARATH (Q9M391) Machado-Joseph disease... 27 6.8
TC12365 similar to UP|Q39892 (Q39892) Nucleosome assembly protei... 27 6.8
>AV427422
Length = 417
Score = 161 bits (407), Expect = 2e-40
Identities = 76/137 (55%), Positives = 103/137 (74%), Gaps = 1/137 (0%)
Frame = +1
Query: 18 GLPKSKGKETILVVVDRLTKFAHFLPLCHLFSASEVAAGFIQEIIRLHGFPSTIVSDHDS 77
GLPKSKG E +LVVVDRL+KF+HF+PL H ++A +A F++E++RLHG P +IVSD D
Sbjct: 7 GLPKSKGYEAVLVVVDRLSKFSHFVPLKHPYTAKVIADIFVREVVRLHGVPLSIVSDRDP 186
Query: 78 -FPE*FLEVLIQGAGTQLKFSSAYHPQTDGQTEVVNRCLEAYLRRLIWTCPKNWSNCLAW 136
F F + L + GT+LK S+AYHP++DGQTEVVNRCLE YLR I PK+W++ + W
Sbjct: 187 LFMSNFWKELFKMQGTKLKMSTAYHPESDGQTEVVNRCLETYLRCFIADQPKSWAHWVPW 366
Query: 137 AEFWFNTNYNTSRNMSP 153
AE+W+NT+Y+ S +P
Sbjct: 367 AEYWYNTSYHVSTGQTP 417
>BP055130
Length = 567
Score = 104 bits (259), Expect(2) = 6e-25
Identities = 53/112 (47%), Positives = 72/112 (63%), Gaps = 1/112 (0%)
Frame = +2
Query: 24 GKETILVVVDRLTKFAHFLPLCHLFSASEVAAGFIQEIIRLHGFPSTIVSDHD-SFPE*F 82
G I+VVVDRL+K+ HF P +++S+VA F+ I++LHG P IVSD D +F F
Sbjct: 179 GFTVIIVVVDRLSKYGHFAPHRANYTSSQVAETFVSTIVKLHGMPRAIVSDRDKAFTSAF 358
Query: 83 LEVLIQGAGTQLKFSSAYHPQTDGQTEVVNRCLEAYLRRLIWTCPKNWSNCL 134
+ + GT L SS+YHPQTDGQTE +N+CLE YLR + P+ W + L
Sbjct: 359 WKHFFKLHGTTLNMSSSYHPQTDGQTEALNKCLELYLRCFVHETPRLWVSYL 514
Score = 26.2 bits (56), Expect(2) = 6e-25
Identities = 8/16 (50%), Positives = 13/16 (81%)
Frame = +3
Query: 133 CLAWAEFWFNTNYNTS 148
CL WAE+ +N+++ TS
Sbjct: 513 CLPWAEYCYNSSFQTS 560
>AU089582
Length = 383
Score = 59.3 bits (142), Expect(2) = 7e-13
Identities = 32/76 (42%), Positives = 46/76 (60%), Gaps = 1/76 (1%)
Frame = +2
Query: 49 SASEVAAGFIQEIIRLHGFPSTIVSDHDS-FPE*FLEVLIQGAGTQLKFSSAYHPQTDGQ 107
+AS+ A ++ EI+ LHG P +I+SD + F F GT+LK S+A+HPQTDGQ
Sbjct: 8 AASQYAKIYLDEIVSLHGVPVSIISDRGAQFTSHFWRSFQTALGTRLKMSTAFHPQTDGQ 187
Query: 108 TEVVNRCLEAYLRRLI 123
+E + LE LR +
Sbjct: 188 SERTIQILEDMLRACV 235
Score = 30.4 bits (67), Expect(2) = 7e-13
Identities = 13/32 (40%), Positives = 20/32 (61%)
Frame = +3
Query: 129 NWSNCLAWAEFWFNTNYNTSRNMSPFNALSGQ 160
+W L+ EF +N +Y +S M+PF AL G+
Sbjct: 252 SWDQYLSLMEFAYNNSYRSSI*MAPFEALYGR 347
>BF177840
Length = 410
Score = 67.8 bits (164), Expect = 3e-12
Identities = 40/99 (40%), Positives = 56/99 (56%), Gaps = 1/99 (1%)
Frame = +2
Query: 69 STIVSDHDS-FPE*FLEVLIQGAGTQLKFSSAYHPQTDGQTEVVNRCLEAYLRRLIWTCP 127
++IVSD D+ F F L GT+L +S+ HPQTDGQTEVVN+ L LR ++
Sbjct: 11 TSIVSDRDTKFISHFWRTLWGKVGTKLLYSTTCHPQTDGQTEVVNKTLSTLLRSVLERNL 190
Query: 128 KNWSNCLAWAEFWFNTNYNTSRNMSPFNALSGQDPLSLL 166
K W L EF +N +++ SPF + G +PL+ L
Sbjct: 191 KMWETWLPHIEFAYNRVVHSTTKHSPFEIVYGYNPLTPL 307
>BP054462
Length = 422
Score = 50.8 bits (120), Expect(3) = 3e-12
Identities = 22/28 (78%), Positives = 26/28 (92%)
Frame = +1
Query: 218 VFLKLQPYFRRCLARRINEKLSPRFYGP 245
VFLKLQPY RR LA+++NEKLSPR+YGP
Sbjct: 241 VFLKLQPYRRRSLAKKMNEKLSPRYYGP 324
Score = 36.2 bits (82), Expect(3) = 3e-12
Identities = 16/27 (59%), Positives = 20/27 (73%)
Frame = +1
Query: 142 NTNYNTSRNMSPFNALSGQDPLSLLKG 168
N+NYN S MSPF AL G++P LL+G
Sbjct: 1 NSNYNRSAKMSPFQALYGREPPVLLQG 81
Score = 20.0 bits (40), Expect(3) = 3e-12
Identities = 10/23 (43%), Positives = 12/23 (51%)
Frame = +3
Query: 194 KIC*HDAFFC**A*ARCGVCSW* 216
KI HD C * CG+ +W*
Sbjct: 168 KISGHDEDICK*EEEGCGLSNW* 236
>TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase region
(Fragment), partial (10%)
Length = 572
Score = 48.9 bits (115), Expect = 1e-06
Identities = 25/75 (33%), Positives = 40/75 (53%)
Frame = +2
Query: 89 GAGTQLKFSSAYHPQTDGQTEVVNRCLEAYLRRLIWTCPKNWSNCLAWAEFWFNTNYNTS 148
G G Q++FSS HPQT+GQTE N+ + ++R ++ W + L + +NT +S
Sbjct: 56 GMGIQMRFSSVKHPQTNGQTEAANKVILKGIKRRLYEAEGRWIDELPIVLWSYNTMPQSS 235
Query: 149 RNMSPFNALSGQDPL 163
+PF G D +
Sbjct: 236 IKETPF*LTYGADTM 280
>BP046863
Length = 580
Score = 46.6 bits (109), Expect = 6e-06
Identities = 23/34 (67%), Positives = 27/34 (78%)
Frame = +1
Query: 218 VFLKLQPYFRRCLARRINEKLSPRFYGPLQG*ER 251
VFLKLQPY + LA+R N+KLSPRFYGP + ER
Sbjct: 397 VFLKLQPYKLQNLAQRKNQKLSPRFYGPFKVLER 498
>CB829142
Length = 534
Score = 30.4 bits (67), Expect = 0.47
Identities = 23/57 (40%), Positives = 27/57 (47%), Gaps = 1/57 (1%)
Frame = +2
Query: 273 LRRWWELIS-RFSSCLKCCLRITSS*FSLQMFWTLGKQLRGILRC*FNGRIFLLVRL 328
LRRWW S R SS CL +TSS* G L I C + R LL+R+
Sbjct: 212 LRRWWITESTRMSSTSLACLIVTSS*---------GSHLTLITMCMISERCMLLLRI 355
>TC15372 similar to UP|Q8W206 (Q8W206) CSN complex subunit 6A, partial (16%)
Length = 604
Score = 29.6 bits (65), Expect = 0.80
Identities = 17/43 (39%), Positives = 27/43 (62%), Gaps = 3/43 (6%)
Frame = +1
Query: 95 KFSSAY--HPQTDGQTEVV-NRCLEAYLRRLIWTCPKNWSNCL 134
KF++AY H + G+T + N ++L L+WT K++SNCL
Sbjct: 97 KFNTAYDRHSRRGGRTAFM*NPLGFSFLVFLLWTASKHFSNCL 225
>TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%)
Length = 1008
Score = 28.9 bits (63), Expect = 1.4
Identities = 23/56 (41%), Positives = 31/56 (55%), Gaps = 8/56 (14%)
Frame = -3
Query: 280 ISRFSSCLKCCLRI---TSS*F-----SLQMFWTLGKQLRGILRC*FNGRIFLLVR 327
+SR SSCL CC+RI TS F S+ +F + G G+ R FN +FL +R
Sbjct: 232 LSRASSCLYCCVRIWILTSMFFFLC*TSMLLFTS*G----GLCRTLFNCYLFLPLR 77
>TC19252 similar to UP|Q9FMS6 (Q9FMS6) CCR4-associated factor-like protein,
partial (50%)
Length = 629
Score = 28.5 bits (62), Expect = 1.8
Identities = 11/42 (26%), Positives = 18/42 (42%)
Frame = +1
Query: 114 CLEAYLRRLIWTCPKNWSNCLAWAEFWFNTNYNTSRNMSPFN 155
C Y L+W P W++ + W WF N ++P +
Sbjct: 343 CFSEYQGSLLW--PSRWASQVCWCLVWFRGFLNFLMELNPIS 462
>TC14688
Length = 633
Score = 28.1 bits (61), Expect = 2.3
Identities = 20/65 (30%), Positives = 29/65 (43%), Gaps = 5/65 (7%)
Frame = -3
Query: 116 EAYLRRLIWTCPKNWSNCLAWAEFWFNTNYNTSRNMSPFNA-----LSGQDPLSLLKGLS 170
E L+R IW KN W + N + ++ + FNA LS +P + LK
Sbjct: 490 EEALKREIWDIYKNTVLHKTWKKPSVNNSTKNTQQVGRFNAQDHYGLS*INPPNHLKQNG 311
Query: 171 YHQGW 175
+H GW
Sbjct: 310 HHLGW 296
>BP049555
Length = 555
Score = 28.1 bits (61), Expect = 2.3
Identities = 24/68 (35%), Positives = 31/68 (45%), Gaps = 5/68 (7%)
Frame = -3
Query: 254 TSHSYLPEVRYTWDF----MFPLLRRWWELISRFSSC-LKCCLRITSS*FSLQMFWTLGK 308
TSH+ ++ TW + F LL W L+ F SC L C + F L +F LG
Sbjct: 430 TSHTMQVHIKNTWSWWFRLFFLLLLFWLFLLLLFLSCRLFLCFFLC---FFLCLFLWLGF 260
Query: 309 QLRGILRC 316
LR L C
Sbjct: 259 SLRLFLFC 236
>CB828459
Length = 549
Score = 27.7 bits (60), Expect = 3.0
Identities = 10/30 (33%), Positives = 16/30 (53%)
Frame = -2
Query: 258 YLPEVRYTWDFMFPLLRRWWELISRFSSCL 287
+L +++ W F+F L RWW + CL
Sbjct: 506 FLNDLKNPWSFLFDLSDRWW*KMHWTKKCL 417
>TC12227
Length = 686
Score = 27.7 bits (60), Expect = 3.0
Identities = 14/26 (53%), Positives = 16/26 (60%)
Frame = -1
Query: 77 SFPE*FLEVLIQGAGTQLKFSSAYHP 102
SFPE* L V I A T+ +SA HP
Sbjct: 281 SFPE*LLRVRIDSAKTEADSNSARHP 204
>BP051766
Length = 457
Score = 27.7 bits (60), Expect = 3.0
Identities = 9/18 (50%), Positives = 14/18 (77%)
Frame = +2
Query: 276 WWELISRFSSCLKCCLRI 293
WW+ I +SSCL+C L++
Sbjct: 44 WWKPIRCWSSCLRCSLQV 97
>TC16971 weakly similar to UP|O23161 (O23161) Receptor kinase-like protein
, partial (12%)
Length = 573
Score = 27.7 bits (60), Expect = 3.0
Identities = 11/28 (39%), Positives = 13/28 (46%)
Frame = -3
Query: 120 RRLIWTCPKNWSNCLAWAEFWFNTNYNT 147
RR W KNW + W FW N + T
Sbjct: 355 RRRRWGLFKNW*HLFHWWSFWRNATHTT 272
>AV777366
Length = 637
Score = 26.9 bits (58), Expect = 5.2
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = -2
Query: 127 PKNWSNCLAWAEFWF 141
P+NWS CL EFW+
Sbjct: 282 PENWSLCLC*VEFWW 238
>TC13517 similar to UP|MJDL_ARATH (Q9M391) Machado-Joseph disease-like
protein (MJD1a-like) (Ataxin-3 homolog), partial (37%)
Length = 430
Score = 26.6 bits (57), Expect = 6.8
Identities = 13/28 (46%), Positives = 18/28 (63%)
Frame = -1
Query: 274 RRWWELISRFSSCLKCCLRITSS*FSLQ 301
RR W L+ R ++CL C L +S * S+Q
Sbjct: 166 RRAWTLVPRGTTCLHCSLLPSS**CSIQ 83
>TC12365 similar to UP|Q39892 (Q39892) Nucleosome assembly protein 1,
partial (47%)
Length = 621
Score = 26.6 bits (57), Expect = 6.8
Identities = 16/46 (34%), Positives = 21/46 (44%)
Frame = +1
Query: 71 IVSDHDSFPE*FLEVLIQGAGTQLKFSSAYHPQTDGQTEVVNRCLE 116
I HD F FLE + A + K+ S Y P + E+VN E
Sbjct: 259 IQGQHDEFEAKFLE---ERAALEAKYQSLYQPLYTKRFEIVNGVTE 387
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.349 0.154 0.574
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,843,388
Number of Sequences: 28460
Number of extensions: 130913
Number of successful extensions: 1316
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 1302
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1312
length of query: 354
length of database: 4,897,600
effective HSP length: 91
effective length of query: 263
effective length of database: 2,307,740
effective search space: 606935620
effective search space used: 606935620
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.8 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0147a.4