BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0142.1
(142 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP030794 192 1e-50
AW428785 53 2e-08
TC15911 44 1e-05
TC15001 similar to UP|GLE1_YEAST (Q12315) RNA export factor GLE1... 33 0.019
TC19909 similar to GB|AAP21292.1|30102748|BT006484 At1g67530 {Ar... 30 0.16
CN824962 30 0.21
TC17245 similar to UP|V721_ARATH (Q9ZTW3) Vesicle-associated mem... 29 0.36
TC19478 similar to UP|Q8L869 (Q8L869) RanGAP1 interacting protei... 28 0.61
TC10196 homologue to UP|Q39804 (Q39804) BiP isoform B, partial (... 28 0.79
TC16907 similar to UP|Q9LJM3 (Q9LJM3) Genomic DNA, chromosome 3,... 27 1.4
TC10393 27 1.8
BP042574 26 3.0
AV428942 25 3.9
BP056026 25 3.9
TC10400 25 3.9
TC18869 similar to UP|Q9SIR1 (Q9SIR1) At2g25320 protein, partial... 25 5.2
TC8273 UP|BAD11134 (BAD11134) Glutamate-rich protein, complete 25 5.2
AV406612 25 6.7
TC10973 similar to UP|P1_ARATH (Q39172) Probable NADP-dependent ... 25 6.7
BP067368 25 6.7
>BP030794
Length = 513
Score = 192 bits (489), Expect = 1e-50
Identities = 95/135 (70%), Positives = 118/135 (87%)
Frame = +1
Query: 8 SVREKKMASYIVALEELRKKAFEEDLFLNIRHPEGTTTIAELIRTLLEEDLRPELERDLR 67
S+ K+MASYIVALEE R+KAFEED FLNIRHP+GT TI EL + LLEEDLRPELE+DL+
Sbjct: 82 SIH*KEMASYIVALEEFRRKAFEED*FLNIRHPDGTRTIHELTKALLEEDLRPELEKDLK 261
Query: 68 EFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEILERDELKDRLSNIQHALERLEKDR 127
EFLAFVEE+QELS+LEL LL+EKE +E ++KT E+ LE+ EL +L NI++ALERLEK+R
Sbjct: 262 EFLAFVEEIQELSQLELMLLEEKESVEGRIKTIEDSLEKTELSIKLGNIEYALERLEKER 441
Query: 128 SEQRQECRRMRRDPP 142
++QR+ECRRMR+DPP
Sbjct: 442 ADQRRECRRMRKDPP 486
>AW428785
Length = 214
Score = 52.8 bits (125), Expect = 2e-08
Identities = 24/28 (85%), Positives = 25/28 (88%)
Frame = +2
Query: 115 NIQHALERLEKDRSEQRQECRRMRRDPP 142
N ALERLEK+RSEQRQECRRMRRDPP
Sbjct: 2 NSARALERLEKERSEQRQECRRMRRDPP 85
>TC15911
Length = 774
Score = 43.5 bits (101), Expect = 1e-05
Identities = 18/37 (48%), Positives = 28/37 (75%)
Frame = -1
Query: 106 RDELKDRLSNIQHALERLEKDRSEQRQECRRMRRDPP 142
++EL +L N+++ RLE R+EQR+ECR M+R+PP
Sbjct: 159 QEELSRKLCNLEYRQYRLELKRTEQRKECREMKRNPP 49
>TC15001 similar to UP|GLE1_YEAST (Q12315) RNA export factor GLE1, partial
(4%)
Length = 988
Score = 33.1 bits (74), Expect = 0.019
Identities = 34/137 (24%), Positives = 59/137 (42%), Gaps = 7/137 (5%)
Frame = +2
Query: 10 REKKMASYIVALEELRKKAFEEDLFLNIRHPEGTTTIAELIRTLLEE-------DLRPEL 62
R +K A + +EE K EE + + + + I+ LEE ++ +L
Sbjct: 356 RRRKEAE-LKLIEEETAKRVEEAIRKRVEESLNSEEVQVEIQRRLEEGRKRLIVEVAVQL 532
Query: 63 ERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEILERDELKDRLSNIQHALER 122
E++ L E +E +R KEKE L+ L+ + +E + ++ L + ER
Sbjct: 533 EKEKEAALIEAREKEEQAR------KEKEDLDRMLEENRKKIEEAQRREALEQQRREEER 694
Query: 123 LEKDRSEQRQECRRMRR 139
+ QRQ+ MRR
Sbjct: 695 YRELEEIQRQKEEAMRR 745
Score = 27.7 bits (60), Expect = 0.79
Identities = 24/74 (32%), Positives = 33/74 (44%), Gaps = 3/74 (4%)
Frame = +2
Query: 55 EEDLRPE---LERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEILERDELKD 111
EE R E L+R L E +EE Q LE ++ R EE+ + EEI + E
Sbjct: 575 EEQARKEKEDLDRMLEENRKKIEEAQRREALE-----QQRREEERYRELEEIQRQKEEAM 739
Query: 112 RLSNIQHALERLEK 125
R + ERL +
Sbjct: 740 RRKKQEEEQERLNQ 781
>TC19909 similar to GB|AAP21292.1|30102748|BT006484 At1g67530 {Arabidopsis
thaliana;}, partial (18%)
Length = 617
Score = 30.0 bits (66), Expect = 0.16
Identities = 18/48 (37%), Positives = 28/48 (57%), Gaps = 6/48 (12%)
Frame = +2
Query: 84 LKLLKEKERLEEKLKTAEEILER------DELKDRLSNIQHALERLEK 125
LK K K LE+ LK E+I+ + DE+ + L+ ++ AL+ LEK
Sbjct: 452 LKFEKAKCALEDSLKQVEDIVPQSIGCQIDEIVNELTGLEFALDPLEK 595
>CN824962
Length = 673
Score = 29.6 bits (65), Expect = 0.21
Identities = 26/100 (26%), Positives = 50/100 (50%), Gaps = 7/100 (7%)
Frame = +1
Query: 48 ELIRTLLEEDLRPELERDLREFLAF-----VEEVQELSR--LELKLLKEKERLEEKLKTA 100
E+I TL + +LE+ ++E + ++ ++LS ELK+L+ + +KLK
Sbjct: 322 EVIVTLFHQI--KDLEKQVKEVKEWAHKKAMQAARKLSNDLTELKMLRMEREETQKLKKG 495
Query: 101 EEILERDELKDRLSNIQHALERLEKDRSEQRQECRRMRRD 140
++ LE +K RLS +++AL + RR+ +
Sbjct: 496 KQALEDTTMK-RLSEMENALRKASGQVDRANAAVRRLETE 612
>TC17245 similar to UP|V721_ARATH (Q9ZTW3) Vesicle-associated membrane
protein 721 (AtVAMP721) (v-SNARE synaptobrevin 7B)
(AtVAMP7B), partial (51%)
Length = 627
Score = 28.9 bits (63), Expect = 0.36
Identities = 18/79 (22%), Positives = 43/79 (53%), Gaps = 4/79 (5%)
Frame = +2
Query: 59 RPELERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEILERDE----LKDRLS 114
RP+L+ ++ + EEV +L++++ ++ + K + E + E++L+R E L D+
Sbjct: 113 RPKLKEHMQYCVDHPEEVSKLAKVKAQVSEVKGVMMENI---EKVLDRGEKIELLVDKTE 283
Query: 115 NIQHALERLEKDRSEQRQE 133
N+ H + ++ R++
Sbjct: 284 NLHHQAQDFRNSGTKIRRK 340
>TC19478 similar to UP|Q8L869 (Q8L869) RanGAP1 interacting protein, partial
(12%)
Length = 551
Score = 28.1 bits (61), Expect = 0.61
Identities = 21/51 (41%), Positives = 27/51 (52%), Gaps = 1/51 (1%)
Frame = +1
Query: 77 QELSRLELKLLKEKERLEEKLKTAEEILERDELKDRLSNIQHALE-RLEKD 126
QE+ + LKE E L EKL + E LE + LSN+ H+ RLE D
Sbjct: 25 QEMEKKMADTLKEMESLREKLGSLEFSLEE---ANHLSNLVHSDNVRLEHD 168
>TC10196 homologue to UP|Q39804 (Q39804) BiP isoform B, partial (17%)
Length = 562
Score = 27.7 bits (60), Expect = 0.79
Identities = 25/99 (25%), Positives = 45/99 (45%)
Frame = +2
Query: 5 EQFSVREKKMASYIVALEELRKKAFEEDLFLNIRHPEGTTTIAELIRTLLEEDLRPELER 64
E+F+ +KK+ I A L E N+++ + + + LE D + ++E
Sbjct: 2 EEFAEEDKKVKERIDARHGL------ETYIYNMKNQ---VSEKDKLADKLESDEKEKIET 154
Query: 65 DLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEEI 103
++E L ++++ Q EKE EEKLK E +
Sbjct: 155 AVKEALEWLDDNQSA---------EKEEYEEKLKEVEAV 244
>TC16907 similar to UP|Q9LJM3 (Q9LJM3) Genomic DNA, chromosome 3, P1 clone:
MMB12, partial (14%)
Length = 855
Score = 26.9 bits (58), Expect = 1.4
Identities = 29/108 (26%), Positives = 54/108 (49%), Gaps = 13/108 (12%)
Frame = +3
Query: 44 TTIAELI-RTLLEEDLRPELER-----------DLREFLAFVEEVQELSRLELKLL-KEK 90
T +A+L+ TL L P ER +RE+ E++ L + ++ K
Sbjct: 6 TKLADLLDSTLWNRRLAPSSERIVYGLVQQIFHGIREYFLVSTELKFNCFLLMPIVDKLP 185
Query: 91 ERLEEKLKTAEEILERDELKDRLSNIQHALERLEKDRSEQRQECRRMR 138
RL E L++A E + D + D ++N+QH+L + ++D + + +R+R
Sbjct: 186 ARLREDLESAFED-DLDNVFD-ITNLQHSLGQQKRDTEIELKRIKRLR 323
>TC10393
Length = 1063
Score = 26.6 bits (57), Expect = 1.8
Identities = 30/103 (29%), Positives = 45/103 (43%), Gaps = 8/103 (7%)
Frame = +1
Query: 5 EQFSVREKKMASYIVALEELRKKAFEEDLFLNIRHPEGTTTIAELIRTL-LEEDLRPELE 63
E S K + ++L K E L P T A LI + + LR E +
Sbjct: 409 ESCSATSSKACREKLRRDKLNDKFVELGSILEPGRPPKTDKAAILIDAVRMVTQLRGEAQ 588
Query: 64 RDLREFLAFVEEVQELS------RLELKLLK-EKERLEEKLKT 99
+ + E+++EL R E + LK EKERLE++LK+
Sbjct: 589 KMKDTNMGLQEKIKELKTEKNELRDEKQRLKTEKERLEQQLKS 717
>BP042574
Length = 505
Score = 25.8 bits (55), Expect = 3.0
Identities = 27/96 (28%), Positives = 41/96 (42%)
Frame = +3
Query: 18 IVALEELRKKAFEEDLFLNIRHPEGTTTIAELIRTLLEEDLRPELERDLREFLAFVEEVQ 77
+V +E +F +D EG+ +E+ ++EE L + DL V
Sbjct: 189 VVDIESASSGSFNDD-----SDDEGSLP-SEIDDKVIEEPLTEQEIEDL---------VS 323
Query: 78 ELSRLELKLLKEKERLEEKLKTAEEILERDELKDRL 113
EL +E K + +E LEE+ E R ELK L
Sbjct: 324 ELLEVESKAAEAQEALEEESLAKVESEVRQELKQTL 431
>AV428942
Length = 362
Score = 25.4 bits (54), Expect = 3.9
Identities = 17/52 (32%), Positives = 27/52 (51%)
Frame = +3
Query: 8 SVREKKMASYIVALEELRKKAFEEDLFLNIRHPEGTTTIAELIRTLLEEDLR 59
S R + ++S I A + +KK ++ + EG EL+ LLEEDL+
Sbjct: 102 SSRPRNLSSCIGAADSRKKKNKSQN-----QGKEGEEDAVELLLKLLEEDLK 242
>BP056026
Length = 405
Score = 25.4 bits (54), Expect = 3.9
Identities = 16/39 (41%), Positives = 23/39 (58%)
Frame = -3
Query: 55 EEDLRPELERDLREFLAFVEEVQELSRLELKLLKEKERL 93
+EDLR + ERDLR F SR+EL+ ++K R+
Sbjct: 184 DEDLRKKNERDLRNF-------NWDSRIELEHKEKKTRI 89
>TC10400
Length = 1004
Score = 25.4 bits (54), Expect = 3.9
Identities = 37/134 (27%), Positives = 53/134 (38%), Gaps = 16/134 (11%)
Frame = +2
Query: 22 EELRKKAFEEDLFLNIRHPEGTTTIAELIRTLLEEDLRPELERDLREFLAFVE-EVQELS 80
+E R+K + + PE T I L RTL + +LE L EFL+ + E+ E
Sbjct: 26 KEKREKMVKASEIDGVTVPEAT--IESLNRTLHSVE---QLEAQLPEFLSLSDPELLE-- 184
Query: 81 RLELKLLKEKERLEEKLKTAEEILE---------------RDELKDRLSNIQHALERLEK 125
++ LL L K + + EL DR+S Q LE L +
Sbjct: 185 --QMPLLHRAHSLFSLAKLTSTLFSLKLRCRGINPNDHPFKSEL-DRISKFQKKLELLPR 355
Query: 126 DRSEQRQECRRMRR 139
QRQ+ R R
Sbjct: 356 LSEAQRQDMRNTSR 397
>TC18869 similar to UP|Q9SIR1 (Q9SIR1) At2g25320 protein, partial (8%)
Length = 381
Score = 25.0 bits (53), Expect = 5.2
Identities = 17/58 (29%), Positives = 29/58 (49%), Gaps = 5/58 (8%)
Frame = +1
Query: 88 KEKERLEEKLKTAEEILE-----RDELKDRLSNIQHALERLEKDRSEQRQECRRMRRD 140
+EK+ L E+++ E LE RD+ K +LS + L+ D Q + + +RD
Sbjct: 7 REKKELTEQVQEIESQLEWLRSERDDEKVKLSAEKKVLQDRLHDADTQLSQLKSRKRD 180
>TC8273 UP|BAD11134 (BAD11134) Glutamate-rich protein, complete
Length = 923
Score = 25.0 bits (53), Expect = 5.2
Identities = 24/98 (24%), Positives = 47/98 (47%)
Frame = +1
Query: 43 TTTIAELIRTLLEEDLRPELERDLREFLAFVEEVQELSRLELKLLKEKERLEEKLKTAEE 102
T + E + E+ PE++ + + VEE +E + E + E+E+ E+ K AE
Sbjct: 310 TKEVTEETKPEAEKTEEPEVKPEEVKEEPIVEETKETAATEAAPV-EEEKSSEESKAAET 486
Query: 103 ILERDELKDRLSNIQHALERLEKDRSEQRQECRRMRRD 140
+E + + A+E+ E S Q EC +++++
Sbjct: 487 TVE--------ATTEVAIEKTEA*TSGQ-YEC*KLKKE 573
>AV406612
Length = 313
Score = 24.6 bits (52), Expect = 6.7
Identities = 9/25 (36%), Positives = 13/25 (52%)
Frame = -1
Query: 118 HALERLEKDRSEQRQECRRMRRDPP 142
H L + ++S EC+R R PP
Sbjct: 253 HLLPHSQNEQSSYLAECKRRNRSPP 179
>TC10973 similar to UP|P1_ARATH (Q39172) Probable NADP-dependent
oxidoreductase P1 , partial (28%)
Length = 663
Score = 24.6 bits (52), Expect = 6.7
Identities = 8/24 (33%), Positives = 15/24 (62%)
Frame = -1
Query: 104 LERDELKDRLSNIQHALERLEKDR 127
++ D+ DR QH+ ++L KD+
Sbjct: 402 MKEDDCNDRTKRAQHSTQKLTKDK 331
>BP067368
Length = 322
Score = 24.6 bits (52), Expect = 6.7
Identities = 18/68 (26%), Positives = 32/68 (46%)
Frame = -2
Query: 73 VEEVQELSRLELKLLKEKERLEEKLKTAEEILERDELKDRLSNIQHALERLEKDRSEQRQ 132
V+ +E SR +K K+ ER + ++++ +RD +DR ++DR+ R
Sbjct: 237 VDRDREKSRERVKD-KDGERERSRERSSDRARDRDHREDR--------HHRDRDRTRDRD 85
Query: 133 ECRRMRRD 140
R RD
Sbjct: 84 RDRERERD 61
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.135 0.349
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,451,673
Number of Sequences: 28460
Number of extensions: 11339
Number of successful extensions: 98
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 97
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 98
length of query: 142
length of database: 4,897,600
effective HSP length: 81
effective length of query: 61
effective length of database: 2,592,340
effective search space: 158132740
effective search space used: 158132740
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)
Lotus: description of TM0142.1