BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0137.2
(210 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC16696 similar to UP|Q9AR81 (Q9AR81) Germin-like protein precur... 394 e-111
TC8987 similar to UP|ABPA_PRUPE (Q9ZRA4) Auxin-binding protein A... 280 1e-76
TC11913 weakly similar to UP|ABPA_PRUPE (Q9ZRA4) Auxin-binding p... 170 2e-43
TC9089 similar to UP|RHRE_PEA (Q9S8P4) Rhicadhesin receptor prec... 131 8e-32
BI418455 100 2e-22
TC11836 similar to GB|AAB51577.1|1755176|ATU75199 germin-like pr... 85 1e-17
BP079498 57 3e-09
AV415918 56 4e-09
TC9088 similar to UP|RHRE_PEA (Q9S8P4) Rhicadhesin receptor prec... 45 9e-06
TC16753 similar to UP|Q9SIA7 (Q9SIA7) Legumin-like protein, part... 34 0.016
TC14962 similar to GB|AAP37696.1|30725348|BT008337 At5g15120 {Ar... 27 2.0
TC8251 similar to GB|AAO24559.1|27808558|BT003127 At5g39890 {Ara... 27 3.4
TC8316 similar to GB|AAO24559.1|27808558|BT003127 At5g39890 {Ara... 27 3.4
TC14498 26 4.4
BU494110 25 7.6
TC11114 similar to UP|ELS_MOUSE (P54320) Elastin precursor (Trop... 25 9.9
>TC16696 similar to UP|Q9AR81 (Q9AR81) Germin-like protein precursor,
partial (96%)
Length = 834
Score = 394 bits (1013), Expect = e-111
Identities = 200/205 (97%), Positives = 203/205 (98%)
Frame = +1
Query: 6 TILFILSLLSLSHASVVDFCVGDLTFPNGPAGYACKKPSKVTADDFAYSGLGIAGNTSNI 65
TILFILSLLSLSHASVVDFCVGDLTFPNGPAGYACKKPSKVTADDFAYSGLGIAGNTS I
Sbjct: 4 TILFILSLLSLSHASVVDFCVGDLTFPNGPAGYACKKPSKVTADDFAYSGLGIAGNTSYI 183
Query: 66 IKAAVTPAFDAQFPGVNGLGISLARLDLASGGVIPLHTHPGASEALVVVQGTICAGFVAS 125
IKAAVTPAFDAQFPGVNGLGISLARLDLASGGVIPLHTHPGASEALVVVQGTICAGFVAS
Sbjct: 184 IKAAVTPAFDAQFPGVNGLGISLARLDLASGGVIPLHTHPGASEALVVVQGTICAGFVAS 363
Query: 126 DNTVYLKTLKKGDVMVFPQGLLHFQINDGGSSALAFVSFSSANPGLQILDFALFKSDFPT 185
DNTVYLKTLKKGDVMVFPQGL+HFQI+DGGSSALAFVSFSSANPGLQILDFALFKSDFP+
Sbjct: 364 DNTVYLKTLKKGDVMVFPQGLVHFQIHDGGSSALAFVSFSSANPGLQILDFALFKSDFPS 543
Query: 186 DLIAATTFLDAAQIKKLKGVLGGSG 210
DLIAAT FLDAAQIKKLKGVLGGSG
Sbjct: 544 DLIAATAFLDAAQIKKLKGVLGGSG 618
>TC8987 similar to UP|ABPA_PRUPE (Q9ZRA4) Auxin-binding protein ABP19a
precursor, partial (97%)
Length = 985
Score = 280 bits (716), Expect = 1e-76
Identities = 141/210 (67%), Positives = 167/210 (79%), Gaps = 1/210 (0%)
Frame = +1
Query: 2 KMVLTILFILSLL-SLSHASVVDFCVGDLTFPNGPAGYACKKPSKVTADDFAYSGLGIAG 60
KM+ I+F+ SLL S SHA+V DFCV DL +GPAGY CK P+ VT+DDF ++GL G
Sbjct: 22 KMLPHIVFLFSLLLSTSHAAVQDFCVADLKGSDGPAGYPCKTPATVTSDDFVFAGLSAPG 201
Query: 61 NTSNIIKAAVTPAFDAQFPGVNGLGISLARLDLASGGVIPLHTHPGASEALVVVQGTICA 120
N +NII AAVTPAF QFPGVNGLG+S ARLD+ GGVIPLHTHPGA+E L+V QG I A
Sbjct: 202 NITNIIGAAVTPAFVGQFPGVNGLGLSAARLDIGPGGVIPLHTHPGANELLIVTQGHITA 381
Query: 121 GFVASDNTVYLKTLKKGDVMVFPQGLLHFQINDGGSSALAFVSFSSANPGLQILDFALFK 180
GF++S +TVY+KTLKKG++MVFPQGLLHFQ+ G + A+ F FSSA+PGLQILDFALF
Sbjct: 382 GFISSASTVYIKTLKKGELMVFPQGLLHFQVAAGKTKAIGFAIFSSASPGLQILDFALFA 561
Query: 181 SDFPTDLIAATTFLDAAQIKKLKGVLGGSG 210
S+F T LI TTFLD A +KKLKGVLGGSG
Sbjct: 562 SNFSTPLITKTTFLDPALVKKLKGVLGGSG 651
>TC11913 weakly similar to UP|ABPA_PRUPE (Q9ZRA4) Auxin-binding protein
ABP19a precursor, partial (61%)
Length = 530
Score = 170 bits (430), Expect = 2e-43
Identities = 78/130 (60%), Positives = 104/130 (80%)
Frame = +1
Query: 81 VNGLGISLARLDLASGGVIPLHTHPGASEALVVVQGTICAGFVASDNTVYLKTLKKGDVM 140
+NGLG+S ARLDL +GGV+P+H+HPGASE ++++QG I GF+++DNTVY KTL KGD++
Sbjct: 16 INGLGLSAARLDLDAGGVVPIHSHPGASELVIILQGRITVGFISTDNTVYQKTLMKGDII 195
Query: 141 VFPQGLLHFQINDGGSSALAFVSFSSANPGLQILDFALFKSDFPTDLIAATTFLDAAQIK 200
V PQGLLHFQ+N GG+ A A ++FSS NP Q++D ALF ++ + L+A TTFLD AQ+K
Sbjct: 196 VIPQGLLHFQLNAGGNRASAVLTFSSTNPVAQLVDVALFGNNLDSGLVARTTFLDLAQVK 375
Query: 201 KLKGVLGGSG 210
KLK V GG G
Sbjct: 376 KLKAVFGGRG 405
>TC9089 similar to UP|RHRE_PEA (Q9S8P4) Rhicadhesin receptor precursor
(Germin-like protein), partial (93%)
Length = 981
Score = 131 bits (330), Expect = 8e-32
Identities = 75/186 (40%), Positives = 105/186 (56%), Gaps = 2/186 (1%)
Frame = +1
Query: 23 DFCVGDLTFPNGPAGYACKKPSKVTADDFAYSGLGIAGNTSNIIKAAVTPAFDAQFPGVN 82
D CV D G+ CK+ SKV A DF + L G T+N + VT A + PG+N
Sbjct: 244 DLCVADPASGVPVNGFTCKEASKVNASDFFSNILAKPGATNNTFGSLVTGANVQKVPGLN 423
Query: 83 GLGISLARLDLASGGVIPLHTHPGASEALVVVQGTICAGFVASDNTVYLKTLKKGDVMVF 142
LG+SLAR+D A G+ P HTHP A+E + V++G + GF+ + N + K + KGD+ VF
Sbjct: 424 TLGVSLARIDYAPDGLNPPHTHPRATEVVFVLEGELDVGFITTGNVLISKHIVKGDIFVF 603
Query: 143 PQGLLHFQINDGGSSALAFVSFSSANPGLQILDFALFKS--DFPTDLIAATTFLDAAQIK 200
P+GLLHFQ N G A +F+S PG Q + LF S P +++ T + QI+
Sbjct: 604 PKGLLHFQKNXGKVPAAVLSAFNSQLPGTQSIATTLFASTPTVPDNVLTKTFQVGTKQIE 783
Query: 201 KLKGVL 206
K+K L
Sbjct: 784 KIKSRL 801
>BI418455
Length = 477
Score = 100 bits (249), Expect = 2e-22
Identities = 69/156 (44%), Positives = 90/156 (57%), Gaps = 13/156 (8%)
Frame = +1
Query: 1 MKMVLTILFILSLLSLSHASVVD------FCVG--DLTFPNGPAGYACKKPSKVTADDFA 52
M++V ++ IL+L S S AS D FCVG D + G CK P+ V A+DF
Sbjct: 16 MEVVYFLVTILALAS-SFASAYDPSPLQDFCVGINDPKYGVFVNGKFCKDPALVKAEDFF 192
Query: 53 YSGLGIAGNTSNIIKAAVTPAFDAQFPGVNGLGISLARLDLASGGVIPLHTHPGASEALV 112
GNTSN + + VTP Q G+N LGISLAR+D A G+ P HTHP A+E L+
Sbjct: 193 KHVE--PGNTSNPLGSKVTPVTVEQLFGLNTLGISLARIDFAPKGLNPPHTHPRATEILI 366
Query: 113 VVQGTICAGFVASDNT-----VYLKTLKKGDVMVFP 143
V++GT+ GFV S+N ++ K L KGDV VFP
Sbjct: 367 VLEGTLYVGFVTSNNANGGNRLFTKVLNKGDVFVFP 474
>TC11836 similar to GB|AAB51577.1|1755176|ATU75199 germin-like protein
{Arabidopsis thaliana;} , partial (56%)
Length = 434
Score = 84.7 bits (208), Expect = 1e-17
Identities = 47/127 (37%), Positives = 72/127 (56%), Gaps = 4/127 (3%)
Frame = +3
Query: 3 MVLTILFILSLLSLSHAS----VVDFCVGDLTFPNGPAGYACKKPSKVTADDFAYSGLGI 58
M L +L +L+L + + AS + D CV DL+ G+ CK S V DF + L
Sbjct: 54 MKLVVLLLLTLTTFTAASDPDSLQDICVADLSSAIKVNGFVCKSASAVKETDFFSAILAK 233
Query: 59 AGNTSNIIKAAVTPAFDAQFPGVNGLGISLARLDLASGGVIPLHTHPGASEALVVVQGTI 118
G T+ + VT A + PG+N LG+S++R+D A GG+ P HTHP A+E + V++G +
Sbjct: 234 PGATNTTFGSVVTGANVEKIPGLNTLGVSISRIDYAPGGLNPPHTHPRATEVVFVLEGQL 413
Query: 119 CAGFVAS 125
GF+ +
Sbjct: 414 DVGFITT 434
>BP079498
Length = 373
Score = 56.6 bits (135), Expect = 3e-09
Identities = 31/70 (44%), Positives = 40/70 (56%)
Frame = -2
Query: 132 KTLKKGDVMVFPQGLLHFQINDGGSSALAFVSFSSANPGLQILDFALFKSDFPTDLIAAT 191
K L KGDV VFP L+HFQ+N G +A+A SS NPG+ + ALFK+ P I+
Sbjct: 369 KVLNKGDVFVFPISLIHFQLNVGSGNAVAIAGLSSQNPGVITIANALFKASPP---ISQE 199
Query: 192 TFLDAAQIKK 201
A Q+ K
Sbjct: 198 VLTKALQVDK 169
>AV415918
Length = 189
Score = 56.2 bits (134), Expect = 4e-09
Identities = 29/59 (49%), Positives = 40/59 (67%), Gaps = 1/59 (1%)
Frame = +3
Query: 7 ILFILSLLSL-SHASVVDFCVGDLTFPNGPAGYACKKPSKVTADDFAYSGLGIAGNTSN 64
ILF+ + LS S+ASV D CV D P+ P+GY C + +T+DDF + GL +AGNT+N
Sbjct: 6 ILFLFTFLSTTSYASVNDLCVADFKGPSTPSGYHCMPLANLTSDDFVFHGL-VAGNTTN 179
>TC9088 similar to UP|RHRE_PEA (Q9S8P4) Rhicadhesin receptor precursor
(Germin-like protein), partial (35%)
Length = 475
Score = 45.1 bits (105), Expect = 9e-06
Identities = 24/73 (32%), Positives = 40/73 (53%), Gaps = 2/73 (2%)
Frame = +3
Query: 136 KGDVMVFPQGLLHFQINDGGSSALAFVSFSSANPGLQILDFALF--KSDFPTDLIAATTF 193
KG++ VFP+GL+H+Q N+G A +F+S PG + ALF K P +++
Sbjct: 3 KGEIFVFPKGLVHYQKNNGYWPASVISAFNSQLPGTVSIPLALFAAKPPVPDNVLTKAFQ 182
Query: 194 LDAAQIKKLKGVL 206
+++K+K L
Sbjct: 183 TSTEEVEKIKANL 221
>TC16753 similar to UP|Q9SIA7 (Q9SIA7) Legumin-like protein, partial (42%)
Length = 534
Score = 34.3 bits (77), Expect = 0.016
Identities = 28/109 (25%), Positives = 48/109 (43%), Gaps = 2/109 (1%)
Frame = +1
Query: 76 AQFPGVNGLGISLARLDLASGGVIPLHTHPGASEALVVVQGTICAGFVASDNTVYLKTLK 135
++ P + I ++L L G L + +S+ V+QGT G V ++ + +K
Sbjct: 157 SELPMLREGNIGASKLALQKNG-FALPRYSDSSKVAYVLQGTGVVGIVLPESEEKVVAIK 333
Query: 136 KGDVMVFPQGLLHFQIN--DGGSSALAFVSFSSANPGLQILDFALFKSD 182
KGD + P G++ + N D L S A+ + DF L S+
Sbjct: 334 KGDALALPLGVVTWWYNKEDTELVVLCLGDTSKAHKAGEFTDFYLTGSN 480
>TC14962 similar to GB|AAP37696.1|30725348|BT008337 At5g15120 {Arabidopsis
thaliana;}, partial (36%)
Length = 632
Score = 27.3 bits (59), Expect = 2.0
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = +1
Query: 93 LASGGVIPLHTHPGASEALVVVQGTI 118
L GVIPLH HPG + ++ GT+
Sbjct: 499 LPPSGVIPLHNHPGMTVFSKLLFGTM 576
>TC8251 similar to GB|AAO24559.1|27808558|BT003127 At5g39890 {Arabidopsis
thaliana;}, partial (41%)
Length = 909
Score = 26.6 bits (57), Expect = 3.4
Identities = 16/43 (37%), Positives = 19/43 (43%)
Frame = +3
Query: 64 NIIKAAVTPAFDAQFPGVNGLGISLARLDLASGGVIPLHTHPG 106
NIIK + + N SL + GVIPLH HPG
Sbjct: 174 NIIKENQRVTYTTVYKCAN---FSLCIFFIPEKGVIPLHNHPG 293
>TC8316 similar to GB|AAO24559.1|27808558|BT003127 At5g39890 {Arabidopsis
thaliana;}, partial (63%)
Length = 1796
Score = 26.6 bits (57), Expect = 3.4
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = +2
Query: 93 LASGGVIPLHTHPGASEALVVVQGTI 118
L GGVIPLH HP + ++ GT+
Sbjct: 593 LPPGGVIPLHNHPEMTVFSKLLFGTM 670
>TC14498
Length = 1136
Score = 26.2 bits (56), Expect = 4.4
Identities = 16/58 (27%), Positives = 27/58 (45%)
Frame = +2
Query: 113 VVQGTICAGFVASDNTVYLKTLKKGDVMVFPQGLLHFQINDGGSSALAFVSFSSANPG 170
+V T+ G S ++ +L+ K + + HF + SS ++ V FSS N G
Sbjct: 38 LVTSTLQTGDKESKSSSFLRPFKSLSTLCKFRNKNHFSLQPFSSSLISGVGFSSPNHG 211
>BU494110
Length = 143
Score = 25.4 bits (54), Expect = 7.6
Identities = 13/29 (44%), Positives = 17/29 (57%)
Frame = +2
Query: 1 MKMVLTILFILSLLSLSHASVVDFCVGDL 29
+ ++L IL S +HA V DFCV DL
Sbjct: 56 INILLLILAAFLSSSNTHAFVSDFCVADL 142
>TC11114 similar to UP|ELS_MOUSE (P54320) Elastin precursor (Tropoelastin),
partial (3%)
Length = 517
Score = 25.0 bits (53), Expect = 9.9
Identities = 12/28 (42%), Positives = 16/28 (56%)
Frame = -3
Query: 72 PAFDAQFPGVNGLGISLARLDLASGGVI 99
PA D G GLG+ L + ++ GGVI
Sbjct: 452 PANDLNGEGARGLGVLLGVVIVSGGGVI 369
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.139 0.402
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,277,130
Number of Sequences: 28460
Number of extensions: 40747
Number of successful extensions: 175
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 171
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of query: 210
length of database: 4,897,600
effective HSP length: 86
effective length of query: 124
effective length of database: 2,450,040
effective search space: 303804960
effective search space used: 303804960
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)
Lotus: description of TM0137.2