BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0134.20
(1583 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP066094 270 9e-76
BP084332 215 4e-56
AV780009 123 2e-28
BP052632 103 2e-22
BP057233 69 8e-12
BP075943 68 1e-11
BP043850 66 4e-11
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 65 6e-11
TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprot... 47 6e-10
AV766665 62 7e-10
AV427921 60 4e-09
AU088980 60 4e-09
AV766088 59 6e-09
BP063694 58 1e-08
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 56 4e-08
TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%) 53 4e-07
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 51 1e-06
TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, p... 50 3e-06
BP033602 49 6e-06
TC19474 weakly similar to UP|Q9FJA1 (Q9FJA1) Similarity to retro... 34 4e-05
>BP066094
Length = 532
Score = 270 bits (690), Expect(3) = 9e-76
Identities = 133/149 (89%), Positives = 139/149 (93%)
Frame = +2
Query: 1162 ARLEAIRLLISFSVNHNIIVHQMDVKSAFLNGYISEEVYVHQPPGFEDEKNPDHVFKLKK 1221
++ EAIRLLISFSVNHNII+HQM+VKSAFLNGYISEEVYVHQPPG EDEKN DH+FKLKK
Sbjct: 86 SKTEAIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPGXEDEKNSDHIFKLKK 265
Query: 1222 SLYGLKQAPRAWYGRLSSFLLDNEFVRGKVDTTLFCKTYKDDI*IKQIYVDDIIFGSANP 1281
SLYGLKQAPRAWY RLSSFLL+NE VRGKVDTTLFCKTYKDDI I QIYVDDIIFGSANP
Sbjct: 266 SLYGLKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANP 445
Query: 1282 SLCKEFSEMMQAELEMSTMGELKYFLGIQ 1310
SLCKEFSEMMQAE EM MGELKYFLGIQ
Sbjct: 446 SLCKEFSEMMQAEFEMRMMGELKYFLGIQ 532
Score = 30.8 bits (68), Expect(3) = 9e-76
Identities = 15/17 (88%), Positives = 15/17 (88%)
Frame = +3
Query: 1147 SQQEGIDYTETFALVAR 1163
SQQ GIDYTETFA VAR
Sbjct: 42 SQQ*GIDYTETFAPVAR 92
Score = 23.1 bits (48), Expect(3) = 9e-76
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +1
Query: 1136 RNKARLVAQG 1145
RNKARLVAQG
Sbjct: 10 RNKARLVAQG 39
>BP084332
Length = 368
Score = 215 bits (548), Expect(2) = 4e-56
Identities = 106/112 (94%), Positives = 108/112 (95%)
Frame = +2
Query: 1466 QSIIALSLAEAEYISAAICSTQMLWTKHQLEDYQILESNIPIYCDNTAAISLSKNPILHS 1525
QS IALS AEAEYISAAICSTQMLW KHQLEDYQILESNIPIYCDNTAAISLSKNPILHS
Sbjct: 32 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILESNIPIYCDNTAAISLSKNPILHS 211
Query: 1526 RAKHIEVKYHFIRDYV*KGVLLLKFVDTDHQWANIFTKPLADDRFNFILKNL 1577
RAKHIEVKYHFIRDYV KGVLLLKFVDTDHQWA+IFTKPLA+DRFNFILKNL
Sbjct: 212 RAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFNFILKNL 367
Score = 21.6 bits (44), Expect(2) = 4e-56
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +1
Query: 1451 CQFLGSNL 1458
CQFLGSNL
Sbjct: 4 CQFLGSNL 27
>AV780009
Length = 529
Score = 123 bits (309), Expect = 2e-28
Identities = 65/168 (38%), Positives = 101/168 (59%), Gaps = 6/168 (3%)
Frame = -1
Query: 1403 AVKRIRRYLKGTTNLGLMYKKTSEYKLSGYCDADYAGDRTERKSTSGNCQFLGSNLVSWA 1462
A R+ RY+KG GL + S KL Y D+D+AG R+S +G FLG++L+SW
Sbjct: 526 AATRVLRYVKGAPAQGLFFSADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSLISWR 347
Query: 1463 SKRQSIIALSLAEAEY--ISAAICSTQMLWTKHQLEDYQILESN----IPIYCDNTAAIS 1516
+K+Q+ ++ S +EAEY ++A +C Q W + +Q L+ N +P++CDN +A+
Sbjct: 346 TKKQTTVSRSSSEAEYRALAATVCEVQ--WLSYL---FQFLKLNVPLPVPLFCDNQSALH 182
Query: 1517 LSKNPILHSRAKHIEVKYHFIRDYV*KGVLLLKFVDTDHQWANIFTKP 1564
++ NP H R KHIE+ H +R + G++ L + T HQ A+IFTKP
Sbjct: 181 IAHNPTFHERTKHIELDCHVVRAKLQAGLIHLLPISTHHQLADIFTKP 38
>BP052632
Length = 489
Score = 103 bits (257), Expect = 2e-22
Identities = 51/123 (41%), Positives = 74/123 (59%)
Frame = -3
Query: 908 KTPYELWKKIKPNFSYFHPFGCVCYALNNKDRLHKFDSKSSKCLLLGYSDRSKGFRIYNT 967
KTPYELW+ +P SYFHPFGC + LN D + KFD+K K +LLGYS SK + ++N+
Sbjct: 487 KTPYELWRGRRPTVSYFHPFGCK*FILNTNDNIGKFDNKFDKRILLGYSSNSKAYIVFNS 308
Query: 968 DAKTIEECIHVRFDDKLDSDQSKLVEKFADMSINVSDKGKAPEEAEPEEDSLEEVGLSDS 1027
+ +EE I+V+FDD +L + F ++ ++ D+ E +P E S EE +
Sbjct: 307 RTQVVEESINVKFDD*SIEATYRLEKDFLNLDLSDQDEEVPTNEGQPSETSHEE---PST 137
Query: 1028 QPQ 1030
QPQ
Sbjct: 136 QPQ 128
>BP057233
Length = 473
Score = 68.6 bits (166), Expect = 8e-12
Identities = 31/77 (40%), Positives = 51/77 (65%)
Frame = -2
Query: 1507 IYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYV*KGVLLLKFVDTDHQWANIFTKPLA 1566
++CDN +A +L+ NP+LH+R+KHIE+ H+IRD V + +++ +V T Q A+ TKPL+
Sbjct: 445 LWCDNLSAKALASNPVLHARSKHIEIDVHYIRDQVLQNEVVVAYVPTTDQIADCLTKPLS 266
Query: 1567 DDRFNFILKNLNMDFCP 1583
RF+ + L + P
Sbjct: 265 HTRFSQLRDKLGVIHSP 215
>BP075943
Length = 547
Score = 67.8 bits (164), Expect = 1e-11
Identities = 44/162 (27%), Positives = 76/162 (46%), Gaps = 5/162 (3%)
Frame = -3
Query: 1269 IYVDDIIFGSANPSLCKEFSEMMQAELEMSTMGELKYFLGIQVDQKPEATYIHQSKYTKE 1328
+YVDD++ + + + + + +GE KYFLG+++ + ++Q KY +
Sbjct: 515 LYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEIARSTSGIVLNQRKYALQ 336
Query: 1329 LLKK---FNMKKSTIAKTPMHPTCILEKEDVSGKVCQKI--YRGMIGSLLYLTASRPDIL 1383
L+ F + + H T +G I YR ++G LLYL +RPDI
Sbjct: 335 LISDSGHFGFQPRFYS----HGTNSQTLGTNTGTPLTDIGSYRRIVGRLLYLNTTRPDIT 168
Query: 1384 FSVHLCARFQSDPRETHLTAVKRIRRYLKGTTNLGLMYKKTS 1425
F+V+ ++F S P + H + +YL G+ GL Y +S
Sbjct: 167 FAVNQLSQFLSAPTDIHEQQLTGFLKYL*GSPGSGLFYPASS 42
>BP043850
Length = 515
Score = 66.2 bits (160), Expect = 4e-11
Identities = 33/93 (35%), Positives = 54/93 (57%), Gaps = 1/93 (1%)
Frame = -1
Query: 1486 TQMLWTKHQLEDYQILESN-IPIYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYV*KG 1544
++++W + L + L+S P++ DNT+AI ++ NP+ H +HIEV H +R+ +
Sbjct: 515 SEIIWLRGLLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYDRR 336
Query: 1545 VLLLKFVDTDHQWANIFTKPLADDRFNFILKNL 1577
V+ L V T Q A+I TK L R NF++ L
Sbjct: 335 VITLPHVSTSVQIADILTKSLTRQRHNFLVSKL 237
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 65.5 bits (158), Expect = 6e-11
Identities = 30/91 (32%), Positives = 52/91 (56%), Gaps = 1/91 (1%)
Frame = +1
Query: 1490 WTKHQLEDYQI-LESNIPIYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYV*KGVLLL 1548
W + L+D ++ E +YCDN +A ++ NP+ H R KHIE+ H +R+ + KG++ L
Sbjct: 4 WLTYLLQDLKVPFEQPALVYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGLIHL 183
Query: 1549 KFVDTDHQWANIFTKPLADDRFNFILKNLNM 1579
+ + A+I+TK L+ F+ I L +
Sbjct: 184 LPISSSEPLADIYTKALSPQNFHQICAKLGL 276
>TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprotein, partial
(4%)
Length = 528
Score = 46.6 bits (109), Expect(2) = 6e-10
Identities = 25/51 (49%), Positives = 32/51 (62%)
Frame = +3
Query: 1529 HIEVKYHFIRDYV*KGVLLLKFVDTDHQWANIFTKPLADDRFNFILKNLNM 1579
+IE KYHF+RD V KG + LK TD Q A+I TK L +RF + LN+
Sbjct: 69 NIETKYHFLRDQVTKGKISLKHCGTDLQVADIMTKGLKTERFRNMRAMLNV 221
Score = 35.4 bits (80), Expect(2) = 6e-10
Identities = 14/22 (63%), Positives = 18/22 (81%)
Frame = +2
Query: 1510 DNTAAISLSKNPILHSRAKHIE 1531
DN +AI L+KNP+ H R+KHIE
Sbjct: 5 DNKSAIDLAKNPVSHGRSKHIE 70
>AV766665
Length = 601
Score = 62.0 bits (149), Expect = 7e-10
Identities = 33/76 (43%), Positives = 44/76 (57%)
Frame = +3
Query: 1402 TAVKRIRRYLKGTTNLGLMYKKTSEYKLSGYCDADYAGDRTERKSTSGNCQFLGSNLVSW 1461
T RI YLK + G +++K + + G+ ADY G +R ST G FL NLV+W
Sbjct: 360 TFTTRIF*YLKANSRRGPLFQKEGKSSMDGFTYADYLGSIVDRLSTMGYYMFLSGNLVTW 539
Query: 1462 ASKRQSIIALSLAEAE 1477
SK+Q+IIA S EAE
Sbjct: 540 RSKQQNIIARSSGEAE 587
>AV427921
Length = 284
Score = 59.7 bits (143), Expect = 4e-09
Identities = 32/80 (40%), Positives = 43/80 (53%)
Frame = +1
Query: 854 THQQNGVIERKNRTLQEMARTMLQETDMAKHFWAEAVNTTCYIQNRISIRPILNKTPYEL 913
T QQNGV ERKNRT+ M R++L + + K F EAV + +I NR + N P E
Sbjct: 22 TPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQNMMPEEA 201
Query: 914 WKKIKPNFSYFHPFGCVCYA 933
W +P +F C+ YA
Sbjct: 202 WSGRQPAVDHFRISRCLAYA 261
>AU088980
Length = 360
Score = 59.7 bits (143), Expect = 4e-09
Identities = 29/120 (24%), Positives = 54/120 (44%)
Frame = +2
Query: 858 NGVIERKNRTLQEMARTMLQETDMAKHFWAEAVNTTCYIQNRISIRPILNKTPYELWKKI 917
N ++ERK+R + + R ++ + + K+ W AV Y+ N + + + PY+
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAYLINXLPSPLLKGQCPYQFLNND 181
Query: 918 KPNFSYFHPFGCVCYALNNKDRLHKFDSKSSKCLLLGYSDRSKGFRIYNTDAKTIEECIH 977
P FG +CY+ K D ++ KC+ LG+ + G+ + + I C H
Sbjct: 182 SPTLLDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKTGTXGYIVMDLPTTXI-XCXH 358
>AV766088
Length = 501
Score = 58.9 bits (141), Expect = 6e-09
Identities = 35/104 (33%), Positives = 56/104 (53%), Gaps = 3/104 (2%)
Frame = -3
Query: 700 QWVWHRRLGHASMSKLDLVRGLPTLKF---SSDALCEACQKGKFTKVPFKAKNVVSTSRP 756
Q +WH RLGH S S L +R + + + +CE+C GK ++PF + N V T P
Sbjct: 313 QSLWHSRLGHPSSSALRYLRSNKFISYELLNYSPVCESCVFGKHVRLPFVSSNNV-TVMP 137
Query: 757 LELLHIDLFGLVKTESIGGKRYGMVIVDDYSCWTWVKFLSRKDE 800
++LH DL+ S G R+ ++ +DD++ + W LS K +
Sbjct: 136 FDILHSDLW-TSPVLSSAGHRFYVLFLDDFTDFLWTFPLSNKSQ 8
>BP063694
Length = 511
Score = 58.2 bits (139), Expect = 1e-08
Identities = 38/117 (32%), Positives = 54/117 (45%), Gaps = 4/117 (3%)
Frame = -2
Query: 702 VWHRRLGHASMSKLDLVRGLPTLKFSS----DALCEACQKGKFTKVPFKAKNVVSTSRPL 757
+WH RLGH + + + L SS C +CQ K ++ F N S L
Sbjct: 360 LWHSRLGHVNFDIIKQLHKHGCLDVSSILPKPICCTSCQMAKSKRLVFHDNNK-RASAVL 184
Query: 758 ELLHIDLFGLVKTESIGGKRYGMVIVDDYSCWTWVKFLSRKDESHSVFSTFIAQVQN 814
+L+H DL G SI G Y ++ VDD+S +TW L RK + V F ++N
Sbjct: 183 DLIHCDLRGPSPVASIDGFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLLRFKVFMEN 13
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 56.2 bits (134), Expect = 4e-08
Identities = 36/145 (24%), Positives = 66/145 (44%), Gaps = 9/145 (6%)
Frame = +2
Query: 1239 SFLLDNEFVRGKVDTTLFCKTYKD-DI*IKQIYVDDIIFGSANPSLCKEFSEMMQAELEM 1297
SF++ + R D + K + D D I +YVDD++ N +E + E +M
Sbjct: 8 SFIMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREFDM 187
Query: 1298 STMGELKYFLGIQV--DQKPEATYIHQSKYTKELLKKFNMKKSTIAKTPMHPTCILEKED 1355
+G LG+Q+ D+K ++ Q Y +++L++FNM+ TP+ L
Sbjct: 188 KDLGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSSSM 367
Query: 1356 VSGKVCQKI------YRGMIGSLLY 1374
+ +++ Y +GSL+Y
Sbjct: 368 IPSSEAERMEMSRVPYASAVGSLMY 442
>TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%)
Length = 550
Score = 52.8 bits (125), Expect = 4e-07
Identities = 28/81 (34%), Positives = 46/81 (56%), Gaps = 1/81 (1%)
Frame = +3
Query: 1487 QMLWTKHQLEDYQILESN-IPIYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYV*KGV 1545
+ LW + L D +I + IYCDN +A+ L+ N + H R ++IE+ H + V G+
Sbjct: 21 EALWLTYALADLRIASLLLVVIYCDNRSALHLAANSVFHKRTENIEIDCHIV*VKVLFGI 200
Query: 1546 LLLKFVDTDHQWANIFTKPLA 1566
L L V + Q A++FTK ++
Sbjct: 201 LHLLHVPSSDQVADVFTKTIS 263
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 51.2 bits (121), Expect = 1e-06
Identities = 53/180 (29%), Positives = 77/180 (42%), Gaps = 4/180 (2%)
Frame = -1
Query: 1322 QSKYTKELLKKFNMKKSTIAKTPMHPTCILEKEDVSGK-VCQKIYRGMIGSLLYLTASRP 1380
Q Y ++LKKF M S T + I GK V Y+ +IGS+ YL R
Sbjct: 739 QKIYVDDILKKFKMTNSKYISTTIGGKEIEAGRRNGGKRVDSTYYKSLIGSVRYLNTVRS 560
Query: 1381 DILFSVHLCARFQSDPRETHLTAVKRIRRYLKGTTNLGL-MYKKTSEYKLSGYCDADYAG 1439
DI+ V L +RF +P + H +R RY+KGT G+ M T L
Sbjct: 559 DIVCGVGLRSRFM-EP*DCH*QGAQRSLRYIKGTLKDGIFMLVITM*NSLD--------- 410
Query: 1440 DRTERKSTSGNCQFLGSNLVSWASKRQSIIALSLAEAEYISAA--ICSTQMLWTKHQLED 1497
T+ + G LG +SW+S +I +L E I ++ +C T + K+ D
Sbjct: 409 --TQIVTRPGYAFHLGRGAISWSS---TISCCTLCE*SIIHSS*ELCYTSNMTNKNS*SD 245
>TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, partial (4%)
Length = 670
Score = 50.1 bits (118), Expect = 3e-06
Identities = 25/91 (27%), Positives = 49/91 (53%), Gaps = 1/91 (1%)
Frame = +1
Query: 1490 WTKHQLEDYQI-LESNIPIYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYV*KGVLLL 1548
W + L+D ++ S +YCD+ +A ++ N + H R KH+++ H +R+ + + L
Sbjct: 13 WLTYILQDLRVPFISPSLLYCDSQSARHIATNAVFHERTKHLDIDCHVVREKLQAKLFHL 192
Query: 1549 KFVDTDHQWANIFTKPLADDRFNFILKNLNM 1579
+ + Q A+I TKPL F+ ++ L +
Sbjct: 193 LPISSVDQTADILTKPLESGPFSHLVSKLGV 285
>BP033602
Length = 533
Score = 48.9 bits (115), Expect = 6e-06
Identities = 30/98 (30%), Positives = 50/98 (50%), Gaps = 5/98 (5%)
Frame = +3
Query: 695 SVNEEQWVWHRRLGHASMSKL-----DLVRGLPTLKFSSDALCEACQKGKFTKVPFKAKN 749
SV ++ +WH RLGH + L DL + + S CE+C K + P+ ++
Sbjct: 255 SVRDQIMLWHNRLGHPNFQYLRHLFPDLFKNVNC----SSLECESCVLAKNQRAPYYSQP 422
Query: 750 VVSTSRPLELLHIDLFGLVKTESIGGKRYGMVIVDDYS 787
SRP L+H D++G K + GKR+ + +DD++
Sbjct: 423 Y-HASRPFYLIHSDVWGPSKITTQFGKRWFVTFIDDHT 533
>TC19474 weakly similar to UP|Q9FJA1 (Q9FJA1) Similarity to retroelement pol
polyprotein, partial (3%)
Length = 517
Score = 34.3 bits (77), Expect(2) = 4e-05
Identities = 20/54 (37%), Positives = 32/54 (59%)
Frame = -2
Query: 1510 DNTAAISLSKNPILHSRAKHIEVKYHFIRDYV*KGVLLLKFVDTDHQWANIFTK 1563
DN AA+ + K RAKHIE+ +HF+ D + +FV+++ Q ++FTK
Sbjct: 354 DNKAALHMPKI*F-SMRAKHIEIDFHFL*DRRLYLDISTRFVNSNDQLTDVFTK 196
Score = 31.2 bits (69), Expect(2) = 4e-05
Identities = 14/25 (56%), Positives = 17/25 (68%)
Frame = -1
Query: 1454 LGSNLVSWASKRQSIIALSLAEAEY 1478
L NL+SW SK I+A S AEAE+
Sbjct: 517 LSDNLISWKSKETIIVARSRAEAEF 443
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.351 0.155 0.541
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,888,665
Number of Sequences: 28460
Number of extensions: 369049
Number of successful extensions: 3326
Number of sequences better than 10.0: 60
Number of HSP's better than 10.0 without gapping: 3261
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3318
length of query: 1583
length of database: 4,897,600
effective HSP length: 102
effective length of query: 1481
effective length of database: 1,994,680
effective search space: 2954121080
effective search space used: 2954121080
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.9 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0134.20