BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0128.9
(1596 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP066094 278 3e-80
BP084332 227 2e-59
AV780009 135 5e-32
BP052632 105 4e-23
BP075943 75 1e-13
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 72 9e-13
BP043850 71 1e-12
BP057233 71 2e-12
BP063694 68 1e-11
AV427921 67 2e-11
TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprot... 48 3e-10
AU088980 63 4e-10
AV766665 62 5e-10
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 59 8e-09
TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%) 57 2e-08
TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, p... 56 4e-08
AV766088 54 1e-07
BP033602 54 3e-07
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 53 3e-07
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 51 1e-06
>BP066094
Length = 532
Score = 278 bits (712), Expect(3) = 3e-80
Identities = 136/149 (91%), Positives = 142/149 (95%)
Frame = +2
Query: 1174 ARLEAIRLLISFSVNHNIVLHQMDVKSAFLNGYISEEVYVHQPPGFEDEKKPDHVFKLKK 1233
++ EAIRLLISFSVNHNI+LHQM+VKSAFLNGYISEEVYVHQPPG EDEK DH+FKLKK
Sbjct: 86 SKTEAIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPGXEDEKNSDHIFKLKK 265
Query: 1234 SLYGLKQAPRAWYERLSSFLLENEFVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANQ 1293
SLYGLKQAPRAWYERLSSFLLENE VRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSAN
Sbjct: 266 SLYGLKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANP 445
Query: 1294 SLCKEFSEMMQAEFEMSMMGELKYFMGIQ 1322
SLCKEFSEMMQAEFEM MMGELKYF+GIQ
Sbjct: 446 SLCKEFSEMMQAEFEMRMMGELKYFLGIQ 532
Score = 37.4 bits (85), Expect(3) = 3e-80
Identities = 17/18 (94%), Positives = 17/18 (94%)
Frame = +3
Query: 1158 YSQQEGIDYTETFAPVAR 1175
YSQQ GIDYTETFAPVAR
Sbjct: 39 YSQQ*GIDYTETFAPVAR 92
Score = 23.1 bits (48), Expect(3) = 3e-80
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +1
Query: 1148 RNKARLVAQG 1157
RNKARLVAQG
Sbjct: 10 RNKARLVAQG 39
>BP084332
Length = 368
Score = 227 bits (578), Expect(2) = 2e-59
Identities = 111/112 (99%), Positives = 112/112 (99%)
Frame = +2
Query: 1478 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILESNIPIYCDNTAAISLSKNPILHS 1537
QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILESNIPIYCDNTAAISLSKNPILHS
Sbjct: 32 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILESNIPIYCDNTAAISLSKNPILHS 211
Query: 1538 RAKHIEVKHHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFNFILKNL 1589
RAKHIEVK+HFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFNFILKNL
Sbjct: 212 RAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFNFILKNL 367
Score = 21.6 bits (44), Expect(2) = 2e-59
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +1
Query: 1463 CQFLGSNL 1470
CQFLGSNL
Sbjct: 4 CQFLGSNL 27
>AV780009
Length = 529
Score = 135 bits (340), Expect = 5e-32
Identities = 69/168 (41%), Positives = 106/168 (63%), Gaps = 6/168 (3%)
Frame = -1
Query: 1415 AVKRILRYLKGTTNLGLMYKKTSEYKLSGYCDADYAGDRTERKSTSGNCQFLGSNLVSWA 1474
A R+LRY+KG GL + S KL Y D+D+AG R+S +G FLG++L+SW
Sbjct: 526 AATRVLRYVKGAPAQGLFFSADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSLISWR 347
Query: 1475 SKRQSTIALSTAEAEY--ISAAICSTQMLWMKHQLEDYQILESN----IPIYCDNTAAIS 1528
+K+Q+T++ S++EAEY ++A +C Q W+ + +Q L+ N +P++CDN +A+
Sbjct: 346 TKKQTTVSRSSSEAEYRALAATVCEVQ--WLSYL---FQFLKLNVPLPVPLFCDNQSALH 182
Query: 1529 LSKNPILHSRAKHIEVKHHFIRDYVQKGVLLLKFVDTDHQWADIFTKP 1576
++ NP H R KHIE+ H +R +Q G++ L + T HQ ADIFTKP
Sbjct: 181 IAHNPTFHERTKHIELDCHVVRAKLQAGLIHLLPISTHHQLADIFTKP 38
>BP052632
Length = 489
Score = 105 bits (263), Expect = 4e-23
Identities = 50/114 (43%), Positives = 71/114 (61%)
Frame = -3
Query: 919 KTPYELWKNIKPNISYFHPFGCVCYVLNTKDRLHKFDAKSSKCLLLGYSERSKGFRFYNT 978
KTPYELW+ +P +SYFHPFGC ++LNT D + KFD K K +LLGYS SK + +N+
Sbjct: 487 KTPYELWRGRRPTVSYFHPFGCK*FILNTNDNIGKFDNKFDKRILLGYSSNSKAYIVFNS 308
Query: 979 DAKTIEESIHVRFDDKLDSDQSKLVEKFADLSINVSDKGKAPEEVEPEEDEPEE 1032
+ +EESI+V+FDD +L + F +L ++ D+ E +P E EE
Sbjct: 307 RTQVVEESINVKFDD*SIEATYRLEKDFLNLDLSDQDEEVPTNEGQPSETSHEE 146
>BP075943
Length = 547
Score = 74.7 bits (182), Expect = 1e-13
Identities = 44/159 (27%), Positives = 78/159 (48%), Gaps = 2/159 (1%)
Frame = -3
Query: 1281 IYVDDIIFGSANQSLCKEFSEMMQAEFEMSMMGELKYFMGIQVDQTPEGTYIHQSKYTKE 1340
+YVDD++ + + + +F + +GE KYF+G+++ ++ G ++Q KY +
Sbjct: 515 LYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEIARSTSGIVLNQRKYALQ 336
Query: 1341 LLKKFNMLESTVAKTPMHPTCILEKEYKSGKVCQKL--YRGMIGSLLYLTASRPDILFSV 1398
L+ + H T +G + YR ++G LLYL +RPDI F+V
Sbjct: 335 LISDSGHF-GFQPRFYSHGTNSQTLGTNTGTPLTDIGSYRRIVGRLLYLNTTRPDITFAV 159
Query: 1399 HLCARFQSDPRETHLTAVKRILRYLKGTTNLGLMYKKTS 1437
+ ++F S P + H + L+YL G+ GL Y +S
Sbjct: 158 NQLSQFLSAPTDIHEQQLTGFLKYL*GSPGSGLFYPASS 42
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 71.6 bits (174), Expect = 9e-13
Identities = 32/91 (35%), Positives = 54/91 (59%), Gaps = 1/91 (1%)
Frame = +1
Query: 1502 WMKHQLEDYQI-LESNIPIYCDNTAAISLSKNPILHSRAKHIEVKHHFIRDYVQKGVLLL 1560
W+ + L+D ++ E +YCDN +A ++ NP+ H R KHIE+ H +R+ +QKG++ L
Sbjct: 4 WLTYLLQDLKVPFEQPALVYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGLIHL 183
Query: 1561 KFVDTDHQWADIFTKPLAEDRFNFILKNLNM 1591
+ + ADI+TK L+ F+ I L +
Sbjct: 184 LPISSSEPLADIYTKALSPQNFHQICAKLGL 276
>BP043850
Length = 515
Score = 71.2 bits (173), Expect = 1e-12
Identities = 34/93 (36%), Positives = 55/93 (58%), Gaps = 1/93 (1%)
Frame = -1
Query: 1498 TQMLWMKHQLEDYQILESN-IPIYCDNTAAISLSKNPILHSRAKHIEVKHHFIRDYVQKG 1556
++++W++ L + L+S P++ DNT+AI ++ NP+ H +HIEV H +R+ +
Sbjct: 515 SEIIWLRGLLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYDRR 336
Query: 1557 VLLLKFVDTDHQWADIFTKPLAEDRFNFILKNL 1589
V+ L V T Q ADI TK L R NF++ L
Sbjct: 335 VITLPHVSTSVQIADILTKSLTRQRHNFLVSKL 237
>BP057233
Length = 473
Score = 70.9 bits (172), Expect = 2e-12
Identities = 32/77 (41%), Positives = 51/77 (65%)
Frame = -2
Query: 1519 IYCDNTAAISLSKNPILHSRAKHIEVKHHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLA 1578
++CDN +A +L+ NP+LH+R+KHIE+ H+IRD V + +++ +V T Q AD TKPL+
Sbjct: 445 LWCDNLSAKALASNPVLHARSKHIEIDVHYIRDQVLQNEVVVAYVPTTDQIADCLTKPLS 266
Query: 1579 EDRFNFILKNLNMDFCP 1595
RF+ + L + P
Sbjct: 265 HTRFSQLRDKLGVIHSP 215
>BP063694
Length = 511
Score = 67.8 bits (164), Expect = 1e-11
Identities = 44/122 (36%), Positives = 61/122 (49%), Gaps = 3/122 (2%)
Frame = -2
Query: 707 VWHRRLGHASMRKISQLSK---LNLVRGLPNLKFASDALCEACQKGKFTKVPFKAKNVVS 763
+WH RLGH + I QL K L++ LP C +CQ K ++ F N
Sbjct: 360 LWHSRLGHVNFDIIKQLHKHGCLDVSSILPK-----PICCTSCQMAKSKRLVFHDNNK-R 199
Query: 764 TSRPLELLHIDLFGPVKIESIGGKRYGMVIVDDYSRWTWVKFLTRKDESHVVFSTFIAQV 823
S L+L+H DL GP + SI G Y ++ VDD+SR+TW L RK + V F +
Sbjct: 198 ASAVLDLIHCDLRGPSPVASIDGFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLLRFKVFM 19
Query: 824 QN 825
+N
Sbjct: 18 EN 13
>AV427921
Length = 284
Score = 67.0 bits (162), Expect = 2e-11
Identities = 33/79 (41%), Positives = 45/79 (56%)
Frame = +1
Query: 865 TPQQNGVVERKNRTLQEMARTMLQETGMAKHFWAEAVNTACYIQNRISVRPILNKTPYEL 924
TPQQNGV ERKNRT+ M R++L +G+ K F EAV + +I NR + N P E
Sbjct: 22 TPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQNMMPEEA 201
Query: 925 WKNIKPNISYFHPFGCVCY 943
W +P + +F C+ Y
Sbjct: 202 WSGRQPAVDHFRISRCLAY 258
>TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprotein, partial
(4%)
Length = 528
Score = 47.8 bits (112), Expect(2) = 3e-10
Identities = 25/51 (49%), Positives = 32/51 (62%)
Frame = +3
Query: 1541 HIEVKHHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFNFILKNLNM 1591
+IE K+HF+RD V KG + LK TD Q ADI TK L +RF + LN+
Sbjct: 69 NIETKYHFLRDQVTKGKISLKHCGTDLQVADIMTKGLKTERFRNMRAMLNV 221
Score = 35.4 bits (80), Expect(2) = 3e-10
Identities = 14/22 (63%), Positives = 18/22 (81%)
Frame = +2
Query: 1522 DNTAAISLSKNPILHSRAKHIE 1543
DN +AI L+KNP+ H R+KHIE
Sbjct: 5 DNKSAIDLAKNPVSHGRSKHIE 70
>AU088980
Length = 360
Score = 62.8 bits (151), Expect = 4e-10
Identities = 29/105 (27%), Positives = 51/105 (47%)
Frame = +2
Query: 869 NGVVERKNRTLQEMARTMLQETGMAKHFWAEAVNTACYIQNRISVRPILNKTPYELWKNI 928
N +VERK+R + + R ++ + + K+ W AV A Y+ N + + + PY+ N
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAYLINXLPSPLLKGQCPYQFLNND 181
Query: 929 KPNISYFHPFGCVCYVLNTKDRLHKFDAKSSKCLLLGYSERSKGF 973
P + FG +CY K D ++ KC+ LG+ + G+
Sbjct: 182 SPTLLDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKTGTXGY 316
>AV766665
Length = 601
Score = 62.4 bits (150), Expect = 5e-10
Identities = 32/76 (42%), Positives = 44/76 (57%)
Frame = +3
Query: 1414 TAVKRILRYLKGTTNLGLMYKKTSEYKLSGYCDADYAGDRTERKSTSGNCQFLGSNLVSW 1473
T RI YLK + G +++K + + G+ ADY G +R ST G FL NLV+W
Sbjct: 360 TFTTRIF*YLKANSRRGPLFQKEGKSSMDGFTYADYLGSIVDRLSTMGYYMFLSGNLVTW 539
Query: 1474 ASKRQSTIALSTAEAE 1489
SK+Q+ IA S+ EAE
Sbjct: 540 RSKQQNIIARSSGEAE 587
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 58.5 bits (140), Expect = 8e-09
Identities = 36/145 (24%), Positives = 71/145 (48%), Gaps = 9/145 (6%)
Frame = +2
Query: 1251 SFLLENEFVRGKVDTTLFCKTYKD-DILIVQIYVDDIIFGSANQSLCKEFSEMMQAEFEM 1309
SF++ + R D + K + D D +I+ +YVDD++ N+ +E + EF+M
Sbjct: 8 SFIMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREFDM 187
Query: 1310 SMMGELKYFMGIQV--DQTPEGTYIHQSKYTKELLKKFNMLESTVAKTP------MHPTC 1361
+G +G+Q+ D+ ++ Q Y +++L++FNM + TP + +
Sbjct: 188 KDLGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSSSM 367
Query: 1362 ILEKEYKSGKVCQKLYRGMIGSLLY 1386
I E + ++ + Y +GSL+Y
Sbjct: 368 IPSSEAERMEMSRVPYASAVGSLMY 442
>TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%)
Length = 550
Score = 57.0 bits (136), Expect = 2e-08
Identities = 29/82 (35%), Positives = 48/82 (58%), Gaps = 1/82 (1%)
Frame = +3
Query: 1499 QMLWMKHQLEDYQILESN-IPIYCDNTAAISLSKNPILHSRAKHIEVKHHFIRDYVQKGV 1557
+ LW+ + L D +I + IYCDN +A+ L+ N + H R ++IE+ H + V G+
Sbjct: 21 EALWLTYALADLRIASLLLVVIYCDNRSALHLAANSVFHKRTENIEIDCHIV*VKVLFGI 200
Query: 1558 LLLKFVDTDHQWADIFTKPLAE 1579
L L V + Q AD+FTK +++
Sbjct: 201 LHLLHVPSSDQVADVFTKTISQ 266
>TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, partial (4%)
Length = 670
Score = 56.2 bits (134), Expect = 4e-08
Identities = 27/91 (29%), Positives = 51/91 (55%), Gaps = 1/91 (1%)
Frame = +1
Query: 1502 WMKHQLEDYQI-LESNIPIYCDNTAAISLSKNPILHSRAKHIEVKHHFIRDYVQKGVLLL 1560
W+ + L+D ++ S +YCD+ +A ++ N + H R KH+++ H +R+ +Q + L
Sbjct: 13 WLTYILQDLRVPFISPSLLYCDSQSARHIATNAVFHERTKHLDIDCHVVREKLQAKLFHL 192
Query: 1561 KFVDTDHQWADIFTKPLAEDRFNFILKNLNM 1591
+ + Q ADI TKPL F+ ++ L +
Sbjct: 193 LPISSVDQTADILTKPLESGPFSHLVSKLGV 285
>AV766088
Length = 501
Score = 54.3 bits (129), Expect = 1e-07
Identities = 34/107 (31%), Positives = 57/107 (52%)
Frame = -3
Query: 705 QWVWHRRLGHASMRKISQLSKLNLVRGLPNLKFASDALCEACQKGKFTKVPFKAKNVVST 764
Q +WH RLGH S + L + N L ++ +CE+C GK ++PF + N V T
Sbjct: 313 QSLWHSRLGHPSSSALRYL-RSNKFISYELLNYSP--VCESCVFGKHVRLPFVSSNNV-T 146
Query: 765 SRPLELLHIDLFGPVKIESIGGKRYGMVIVDDYSRWTWVKFLTRKDE 811
P ++LH DL+ + S G R+ ++ +DD++ + W L+ K +
Sbjct: 145 VMPFDILHSDLWTSPVLSS-AGHRFYVLFLDDFTDFLWTFPLSNKSQ 8
>BP033602
Length = 533
Score = 53.5 bits (127), Expect = 3e-07
Identities = 31/99 (31%), Positives = 54/99 (54%)
Frame = +3
Query: 700 SVDEEQWVWHRRLGHASMRKISQLSKLNLVRGLPNLKFASDALCEACQKGKFTKVPFKAK 759
SV ++ +WH RLGH + + + L +L + + S CE+C K + P+ ++
Sbjct: 255 SVRDQIMLWHNRLGHPNFQYLRHLFP-DLFKNVN----CSSLECESCVLAKNQRAPYYSQ 419
Query: 760 NVVSTSRPLELLHIDLFGPVKIESIGGKRYGMVIVDDYS 798
SRP L+H D++GP KI + GKR+ + +DD++
Sbjct: 420 PY-HASRPFYLIHSDVWGPSKITTQFGKRWFVTFIDDHT 533
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 53.1 bits (126), Expect = 3e-07
Identities = 36/99 (36%), Positives = 49/99 (49%), Gaps = 1/99 (1%)
Frame = -1
Query: 1334 QSKYTKELLKKFNMLESTVAKTPMHPTCILEKEYKSGK-VCQKLYRGMIGSLLYLTASRP 1392
Q Y ++LKKF M S T + I GK V Y+ +IGS+ YL R
Sbjct: 739 QKIYVDDILKKFKMTNSKYISTTIGGKEIEAGRRNGGKRVDSTYYKSLIGSVRYLNTVRS 560
Query: 1393 DILFSVHLCARFQSDPRETHLTAVKRILRYLKGTTNLGL 1431
DI+ V L +RF +P + H +R LRY+KGT G+
Sbjct: 559 DIVCGVGLRSRFM-EP*DCH*QGAQRSLRYIKGTLKDGI 446
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 51.2 bits (121), Expect = 1e-06
Identities = 35/114 (30%), Positives = 59/114 (51%), Gaps = 3/114 (2%)
Frame = +3
Query: 1471 VSWASKRQSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILESNIPIYC---DNTAAI 1527
V+W S+ Q +ALSTAEAE+I+A ++LWMK+ L++ + PI C A
Sbjct: 27 VAWPSRLQKCVALSTAEAEFIAATEACHELLWMKNFLQNAWF--HSHPILCCIVITKALF 200
Query: 1528 SLSKNPILHSRAKHIEVKHHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDR 1581
+L++ + + +RD + +L L+ + TD AD+ TK L ++
Sbjct: 201 TLARILLFIQDPSTLMFVIIGLRDVLNSKLLELEKIHTDDDGADMMTKSLPREK 362
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.332 0.142 0.437
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,523,444
Number of Sequences: 28460
Number of extensions: 363711
Number of successful extensions: 2976
Number of sequences better than 10.0: 64
Number of HSP's better than 10.0 without gapping: 2918
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2967
length of query: 1596
length of database: 4,897,600
effective HSP length: 103
effective length of query: 1493
effective length of database: 1,966,220
effective search space: 2935566460
effective search space used: 2935566460
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.5 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0128.9