BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0125.16
(229 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI418488 131 1e-31
BP041027 91 1e-19
TC12987 similar to UP|Q9SM91 (Q9SM91) Zwh18.1, partial (5%) 75 7e-15
BP069013 44 3e-05
AW719736 39 0.001
AV769974 37 0.003
AW164143 35 0.008
AV766851 35 0.011
BP052706 34 0.018
TC19458 weakly similar to UP|Q9STE8 (Q9STE8) Chloroplast import-... 34 0.018
TC8142 UP|BAD11133 (BAD11133) Glutamate-rich protein, complete 34 0.024
TC14103 similar to UP|Q84XC6 (Q84XC6) Plasma intrinsic protein 2... 32 0.092
TC12588 32 0.092
TC14463 similar to UP|Q9SLF1 (Q9SLF1) Nodulin-like protein (At2g... 32 0.12
BI419523 32 0.12
TC8970 31 0.20
BI419261 31 0.20
TC8309 UP|O22306 (O22306) Nlj21, complete 31 0.20
AV427017 30 0.27
AV419448 30 0.35
>BI418488
Length = 475
Score = 131 bits (329), Expect = 1e-31
Identities = 69/94 (73%), Positives = 73/94 (77%), Gaps = 6/94 (6%)
Frame = +2
Query: 2 FVFGRSACC------QMSMADWFSMVSPSGGIRFKLVDRHAPTAWVIDPVDHRATYRGRV 55
FVF +C QMSMADW SMVSPSGGIR++LVDR APTAWVID V HRATYRGRV
Sbjct: 86 FVFSSESCSSRIIPLQMSMADWCSMVSPSGGIRYELVDRDAPTAWVIDVVGHRATYRGRV 265
Query: 56 MEDRTDERGRLTRGVLRRMELSFNLPPSVRCGPG 89
MEDRTDE LT+ VLRRMELSFNLP SVR G G
Sbjct: 266 MEDRTDECEHLTKEVLRRMELSFNLPSSVRRGLG 367
>BP041027
Length = 527
Score = 91.3 bits (225), Expect = 1e-19
Identities = 48/62 (77%), Positives = 54/62 (86%), Gaps = 2/62 (3%)
Frame = -2
Query: 170 VEEDVLSEEVDVEMGSTRVVRRIVRKEVMDLDTEMITV--DSDSDTDVDNYSPSDEEEEE 227
VEEDVL+ EVDVE G TR VRR VR+EV+DLDTEMIT+ DSDSD+DVD+YSPSDE EEE
Sbjct: 526 VEEDVLTGEVDVETGLTRTVRRTVRREVVDLDTEMITMDSDSDSDSDVDSYSPSDEGEEE 347
Query: 228 EL 229
EL
Sbjct: 346 EL 341
>TC12987 similar to UP|Q9SM91 (Q9SM91) Zwh18.1, partial (5%)
Length = 552
Score = 75.5 bits (184), Expect = 7e-15
Identities = 39/54 (72%), Positives = 46/54 (84%), Gaps = 2/54 (3%)
Frame = +1
Query: 178 EVDVEMGSTRVVRRIVRKEVMDLDTEMITVDSD--SDTDVDNYSPSDEEEEEEL 229
EVDVE GSTR VRR VR+EV+DLDTEMITVDSD S++ ++YSPS +EEEEEL
Sbjct: 4 EVDVETGSTRAVRRTVRREVVDLDTEMITVDSDSASESSGESYSPSSDEEEEEL 165
>BP069013
Length = 470
Score = 43.5 bits (101), Expect = 3e-05
Identities = 20/41 (48%), Positives = 28/41 (67%)
Frame = -1
Query: 11 QMSMADWFSMVSPSGGIRFKLVDRHAPTAWVIDPVDHRATY 51
Q++MAD F+MV PSG R K +D+ + ++DP HRATY
Sbjct: 455 QLTMADRFTMVHPSGESRLKKLDQEDYGSGLVDPAGHRATY 333
>AW719736
Length = 379
Score = 38.5 bits (88), Expect = 0.001
Identities = 16/22 (72%), Positives = 19/22 (85%)
Frame = +3
Query: 11 QMSMADWFSMVSPSGGIRFKLV 32
QMSMA+WF MV PSGG RF+L+
Sbjct: 312 QMSMAEWFEMVVPSGGTRFELL 377
>AV769974
Length = 406
Score = 37.0 bits (84), Expect = 0.003
Identities = 17/20 (85%), Positives = 17/20 (85%)
Frame = -2
Query: 73 RMELSFNLPPSVRCGPGTGR 92
RMELSFNLP S RCG GTGR
Sbjct: 402 RMELSFNLPTSDRCGFGTGR 343
>AW164143
Length = 412
Score = 35.4 bits (80), Expect = 0.008
Identities = 18/46 (39%), Positives = 25/46 (54%)
Frame = +2
Query: 95 PSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASSSRLVEP 140
PSPP S+ P+ A V A +AP PA+A + +ASS + P
Sbjct: 47 PSPPRSTVSAAAPAPAPAPVSASIAPAPASARTVRKMASSKTITIP 184
>AV766851
Length = 445
Score = 35.0 bits (79), Expect = 0.011
Identities = 21/45 (46%), Positives = 23/45 (50%), Gaps = 6/45 (13%)
Frame = +2
Query: 87 GPGTGRGPPSPPSSSSDEEDPSEIEADVD------APVAPLPATA 125
GPGT + PPSPP E S +E D P APLPATA
Sbjct: 272 GPGTHQPPPSPPPPPLLLEPLSAVERDTGEQGSR*RPPAPLPATA 406
>BP052706
Length = 503
Score = 34.3 bits (77), Expect = 0.018
Identities = 16/46 (34%), Positives = 28/46 (60%)
Frame = -2
Query: 1 MFVFGRSACCQMSMADWFSMVSPSGGIRFKLVDRHAPTAWVIDPVD 46
+FVFGRS C+M + +WF MV SG +R ++++ ++ + D
Sbjct: 358 LFVFGRSXNCKM-VNNWFVMVRHSGQVRSEMIEHSDEDSYFMSEKD 224
>TC19458 weakly similar to UP|Q9STE8 (Q9STE8) Chloroplast import-associated
channel homolog (AT3g46740/T6H20_230), partial (10%)
Length = 469
Score = 34.3 bits (77), Expect = 0.018
Identities = 24/72 (33%), Positives = 35/72 (48%), Gaps = 7/72 (9%)
Frame = +1
Query: 88 PGTGRGPPSPPSSSSDEEDPSE---IEADVDAPVAP----LPATAVDLTDVASSSRLVEP 140
P + PP PPSSS+ P EA+V+AP A + + A + T++ +S P
Sbjct: 142 PPSHSPPPPPPSSSTPPLSPPSSPRAEAEVEAPPAEEEVEVGSAAAEATEMEASGPDSSP 321
Query: 141 KREPVVPSASRR 152
+R P S S R
Sbjct: 322 RRRPTPTSHSPR 357
>TC8142 UP|BAD11133 (BAD11133) Glutamate-rich protein, complete
Length = 999
Score = 33.9 bits (76), Expect = 0.024
Identities = 29/102 (28%), Positives = 43/102 (41%), Gaps = 13/102 (12%)
Frame = +1
Query: 95 PSPPSSSSDE------EDPSEIEADVDAPVAPLPATAVDLTDVASSSRLVEPKREPVVPS 148
P+P +++E E+P E E + +APVAP D +V + E K E V P
Sbjct: 238 PAPAEPATEEPKEETTEEPKETETETEAPVAP---ETQDPLEVENKEVTEEAKPEEVKPE 408
Query: 149 ASRRFSFT-------PSRGYRVELLVRVVEEDVLSEEVDVEM 183
+ T P+ E V EE+ +EE VE+
Sbjct: 409 TEKTEEKTEEPKTEEPAATTETESAAPVEEENKPAEEAPVEV 534
>TC14103 similar to UP|Q84XC6 (Q84XC6) Plasma intrinsic protein 2,2, partial
(94%)
Length = 1199
Score = 32.0 bits (71), Expect = 0.092
Identities = 24/70 (34%), Positives = 29/70 (41%), Gaps = 3/70 (4%)
Frame = +1
Query: 67 TRGVLRRMELSFNLPPSVRCGPGTGRGPPS---PPSSSSDEEDPSEIEADVDAPVAPLPA 123
TR + + + PPS GP T PS P SSS+ PS A P+ P P
Sbjct: 133 TRTTMTHHQHRSSTPPSSPSGPSTEPSSPSSSPPCSSSTSPSSPSSATAAKPTPLTP-PT 309
Query: 124 TAVDLTDVAS 133
A L AS
Sbjct: 310 LAAALASSAS 339
>TC12588
Length = 878
Score = 32.0 bits (71), Expect = 0.092
Identities = 17/30 (56%), Positives = 21/30 (69%), Gaps = 1/30 (3%)
Frame = +1
Query: 3 VFGRS-ACCQMSMADWFSMVSPSGGIRFKL 31
VF R+ A CQMS+A F MV PSGG R ++
Sbjct: 295 VFWRTNALCQMSLAG*FMMVHPSGGTRVEM 384
>TC14463 similar to UP|Q9SLF1 (Q9SLF1) Nodulin-like protein
(At2g16660/T24I21.7), partial (40%)
Length = 1122
Score = 31.6 bits (70), Expect = 0.12
Identities = 20/64 (31%), Positives = 29/64 (45%)
Frame = -3
Query: 94 PPSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASSSRLVEPKREPVVPSASRRF 153
PPSPP S + A V +P AP ++ ++ + S SR P +P P+ R
Sbjct: 1006 PPSPPRSPCRHSE----SATVRSPAAPQHPGSISISFL*SKSRPESPPAKPTEPAKPPRT 839
Query: 154 SFTP 157
TP
Sbjct: 838 LATP 827
>BI419523
Length = 548
Score = 31.6 bits (70), Expect = 0.12
Identities = 13/13 (100%), Positives = 13/13 (100%)
Frame = +3
Query: 11 QMSMADWFSMVSP 23
QMSMADWFSMVSP
Sbjct: 510 QMSMADWFSMVSP 548
>TC8970
Length = 609
Score = 30.8 bits (68), Expect = 0.20
Identities = 18/40 (45%), Positives = 22/40 (55%)
Frame = +2
Query: 1 MFVFGRSACCQMSMADWFSMVSPSGGIRFKLVDRHAPTAW 40
MFVFGRSACCQ + F V SG R ++ P+ W
Sbjct: 44 MFVFGRSACCQ-TFNR*FKKVRRSGRTRSEMTTTE-PSTW 157
>BI419261
Length = 591
Score = 30.8 bits (68), Expect = 0.20
Identities = 24/73 (32%), Positives = 30/73 (40%), Gaps = 7/73 (9%)
Frame = +1
Query: 94 PPSPPSSSSDEEDPSEIEADVDAP-------VAPLPATAVDLTDVASSSRLVEPKREPVV 146
PPSPP SS PS +P P P T L+ + +S L PKR
Sbjct: 154 PPSPPQSSQTSTPPSTSVVAPPSPSSSRATATPPPPTTPC*LSGGSGTSSLTAPKR---- 321
Query: 147 PSASRRFSFTPSR 159
PS+ R + P R
Sbjct: 322 PSSWGRSTVRPRR 360
>TC8309 UP|O22306 (O22306) Nlj21, complete
Length = 1063
Score = 30.8 bits (68), Expect = 0.20
Identities = 29/136 (21%), Positives = 50/136 (36%), Gaps = 1/136 (0%)
Frame = +3
Query: 95 PSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVASSSRLVEPKREPVVPSASRRFS 154
P P S + EED + EA P P+ + T+V V EP V
Sbjct: 306 PEPVSEKTKEEDSAVTEASEPEPTEDNPSIEAETTEVVEEVVTVTVTDEPKVEE------ 467
Query: 155 FTPSRGYRVELLVRVVEEDVLSEEVDVEMGST-RVVRRIVRKEVMDLDTEMITVDSDSDT 213
+ G + E ++ V+V+ + KE + E + +
Sbjct: 468 --KTDGEAKKEATETKETKESADPVEVQAPEAGEEPETVTLKEDASKEEEKVAEPEAKEE 641
Query: 214 DVDNYSPSDEEEEEEL 229
V+ +P +++EEE+L
Sbjct: 642 VVNTEAPDEKKEEEKL 689
>AV427017
Length = 429
Score = 30.4 bits (67), Expect = 0.27
Identities = 23/79 (29%), Positives = 29/79 (36%), Gaps = 5/79 (6%)
Frame = +3
Query: 83 SVRCGPGTGRGPPSPPSSSSDEEDPSEIEADVDAPVAPLPATAVDLTDVAS-----SSRL 137
S R P + PPSP SSS P+ + P + T L+ S S L
Sbjct: 165 SARSSPPSSSSPPSPASSSG*SSAPTSSNSASPKPHSLNSPTPTTLSTTISPSTSPSETL 344
Query: 138 VEPKREPVVPSASRRFSFT 156
PS RRF+ T
Sbjct: 345 TAESASTTTPSKRRRFTTT 401
>AV419448
Length = 382
Score = 30.0 bits (66), Expect = 0.35
Identities = 28/73 (38%), Positives = 34/73 (46%), Gaps = 4/73 (5%)
Frame = +2
Query: 82 PSVRCGPGTGRGPPSPPSS---SSDEEDPSEIEADVDAPVAPLPATAVDLTDVASSSRLV 138
PS+ P PPSP +S S+ PS A AP PL A A+ L A+SS
Sbjct: 68 PSLPSPPLHPPTPPSPTASSWTSASAPPPSSPTATPSAPTPPLSA-ALSLASTATSS--- 235
Query: 139 EPKREPVV-PSAS 150
P P+ PSAS
Sbjct: 236 -PSPSPISNPSAS 271
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.315 0.133 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,539,535
Number of Sequences: 28460
Number of extensions: 54147
Number of successful extensions: 704
Number of sequences better than 10.0: 116
Number of HSP's better than 10.0 without gapping: 627
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 680
length of query: 229
length of database: 4,897,600
effective HSP length: 87
effective length of query: 142
effective length of database: 2,421,580
effective search space: 343864360
effective search space used: 343864360
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0125.16