BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0122.9
(460 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV781067 191 3e-49
AU088785 143 5e-35
BP032748 76 1e-14
TC9821 similar to UP|Q84LI7 (Q84LI7) Polygalacturonase-like prot... 74 7e-14
AV770305 55 7e-14
AV422277 70 7e-13
AV775483 65 2e-11
TC12747 weakly similar to UP|Q8LAH2 (Q8LAH2) Polygalacturonase-l... 64 5e-11
AV776486 61 4e-10
BP030183 60 6e-10
AV423118 55 3e-08
TC18673 similar to UP|Q9LWY0 (Q9LWY0) ESTs AU029388(E30287), par... 53 1e-07
AU088950 47 5e-06
BP060510 45 2e-05
AV780084 45 3e-05
TC19633 similar to UP|Q7NET9 (Q7NET9) Glr3789 protein, partial (4%) 44 6e-05
BP042395 41 5e-04
TC11359 37 0.009
BP034892 33 0.076
TC11304 similar to PIR|T04669|T04669 serine O-acetyltransferase ... 33 0.076
>AV781067
Length = 538
Score = 191 bits (484), Expect = 3e-49
Identities = 82/143 (57%), Positives = 113/143 (78%)
Frame = -2
Query: 318 KGTDNGVRIKTWQGGSGSVSGVKFSNIRMVNVRNPIIIDQFYCITEGCTNKTSAVFVSNI 377
K +DNG+RIKTWQGG+GSV+ ++F NI+M NVRN I IDQ+YC+T+ C N+TSAV V+++
Sbjct: 537 KESDNGLRIKTWQGGTGSVTNLRFENIQMENVRNCINIDQYYCLTKECRNQTSAVHVNDV 358
Query: 378 LYTNIKGTYDVRSLPMRFACSDSIPCTHLALSDIELLPAEGDIAHDPFCWNAYGDLQTLT 437
Y NIKGTYDVR+ P+ FACSDS+ CT++ LSD+ELLP EG++ D FCWNAYG +T+T
Sbjct: 357 SYINIKGTYDVRTPPIHFACSDSVACTNITLSDVELLPYEGELLEDSFCWNAYGTQETIT 178
Query: 438 IPPVSCLMQGTPQSWLDHDINGC 460
+PP+ CL +G P+ ++ + C
Sbjct: 177 VPPIDCLREGEPERVIELSLYEC 109
>AU088785
Length = 773
Score = 143 bits (361), Expect = 5e-35
Identities = 90/251 (35%), Positives = 125/251 (48%), Gaps = 4/251 (1%)
Frame = +2
Query: 195 SPVAIRFFMSSNLTVQGLRVKNSPQFNFRFDGCIKVHVESIYITAPKLSPNTDGIHIENT 254
+P ++ F SN+ VQ ++ N F+ C V + + + AP+ SPNTDGIHI ++
Sbjct: 11 NPSSLFFMDXSNVVVQNIKSLNPKGFHMFVTKCTNVRLRKLKLIAPETSPNTDGIHISSS 190
Query: 255 NDVKIYNSMISNGDDCVSIGSGCYDVDIKNITCGPGHGISIGSLGIHNSRACVSNITVRD 314
+V I + I GDDC+S+ G +V I + CGPGHGISIGSLG + V I + +
Sbjct: 191 INVIIARNTIQTGDDCISMIXGSENVFINRLKCGPGHGISIGSLGKYAEXREVKGIXIXN 370
Query: 315 SMMKGTDNGVRIKTWQGG-SGSVSGVKFSNIRMVNVRNPIIIDQFYCITEGCTNKTSAVF 373
S + GT NG+RIK+W G+ S + FSNI M NV+NPIIIDQ Y + C K
Sbjct: 371 SALIGTTNGLRIKSWPDKYGGAASXISFSNITMENVKNPIIIDQXYQCSPNC-QKNHXW* 547
Query: 374 VSNILYTNIKGTYDVRSLPMRFACSDSIPCTHLALSDIELLPAEGDIAHD--PFCWN-AY 430
I + + F C I+L P G+ H P WN +Y
Sbjct: 548 GLXIFTLQMXXELQLHQXAXDFXC-------------IKLYPCHGNYXHKH*PEXWNCSY 688
Query: 431 GDLQTLTIPPV 441
+ I PV
Sbjct: 689 NPXKCSHIKPV 721
>BP032748
Length = 453
Score = 76.3 bits (186), Expect = 1e-14
Identities = 41/93 (44%), Positives = 55/93 (59%), Gaps = 1/93 (1%)
Frame = -2
Query: 322 NGVRIKTWQGG-SGSVSGVKFSNIRMVNVRNPIIIDQFYCITEGCTNKTSAVFVSNILYT 380
NG+RIK+W G+ S + FSNI M NV+NPIIIDQ Y + C K S V + +I +
Sbjct: 452 NGLRIKSWPDKYGGAASEISFSNITMENVKNPIIIDQEYQCSPNCQKKPSLVRIRDIHFA 273
Query: 381 NIKGTYDVRSLPMRFACSDSIPCTHLALSDIEL 413
N+KGT + + F CS PC + L DI+L
Sbjct: 272 NVKGT-TTSPIAVDFRCSKLYPCMGITLRDIDL 177
>TC9821 similar to UP|Q84LI7 (Q84LI7) Polygalacturonase-like protein,
partial (61%)
Length = 1080
Score = 73.6 bits (179), Expect = 7e-14
Identities = 57/195 (29%), Positives = 89/195 (45%), Gaps = 13/195 (6%)
Frame = +3
Query: 169 GEKWWDLPCKPHRQGPHWTTLPGPCDSPVAIRFFMSSNLTVQGLRVKNSPQFNFRFDGCI 228
G WW K H + +T P I S N+ + L + NSP +N C
Sbjct: 15 GALWWK---KFHNKQLQYTR-------PYLIEIMYSDNVQISNLTLVNSPSWNIHPVYCS 164
Query: 229 KVHVESIYITAPKLSPNTDGIHIENTNDVKIYNSMISNGDDCVSIGSGC--YDVD----- 281
V V+ I I AP SPNTDGI+ ++ + +I + I +GDDCV++ SG Y +
Sbjct: 165 NVIVQGITILAPVTSPNTDGINPDSCTNTRIEDCYIVSGDDCVAVKSGWDEYGISYGMPT 344
Query: 282 ----IKNITC-GPGHG-ISIGSLGIHNSRACVSNITVRDSMMKGTDNGVRIKTWQGGSGS 335
I+ +TC P I++GS + ++ D + +++GVRIKT G G
Sbjct: 345 KHLVIRRLTCISPTSAVIALGS----EMSGGIEDVRAEDILAINSESGVRIKTAVGRGGY 512
Query: 336 VSGVKFSNIRMVNVR 350
V + + M ++
Sbjct: 513 VRDIYVRRMTMKTMK 557
>AV770305
Length = 442
Score = 54.7 bits (130), Expect(2) = 7e-14
Identities = 32/120 (26%), Positives = 60/120 (49%), Gaps = 2/120 (1%)
Frame = +2
Query: 101 VPKGFSFIIQS-TIFTGPCQGGIV-FKVEGTLMPPDGPESWPKNISRHQWLVFYRINGMS 158
+P G +F++ S T GPC+ + +++G ++ P ++W + S+ W+ + +N ++
Sbjct: 134 IPGGKTFLVTSITTLPGPCKSSSINIQLQGNIVAPSF-DAWGADKSK--WISIHSVNHLT 304
Query: 159 LEGSGLIDGRGEKWWDLPCKPHRQGPHWTTLPGPCDSPVAIRFFMSSNLTVQGLRVKNSP 218
+ G G IDG G W+ C+ C P ++ F+ + LTV L + NSP
Sbjct: 305 MNGGGKIDGLGSSGWE-RCR-------------TCARPTSLSFYSGNGLTVSNLHLANSP 442
Score = 38.9 bits (89), Expect(2) = 7e-14
Identities = 17/44 (38%), Positives = 26/44 (58%)
Frame = +1
Query: 56 CYNATGIFDVTTFGAVGDGIADDTVSFKMAWDTACQSELPGNVI 99
C I++V +GA GDG +DD+ +F AW AC+S G ++
Sbjct: 1 CLCQNTIYNVMHYGAKGDGKSDDSHAFVSAWKVACESAEVGTLV 132
>AV422277
Length = 454
Score = 70.1 bits (170), Expect = 7e-13
Identities = 39/115 (33%), Positives = 61/115 (52%)
Frame = +2
Query: 162 SGLIDGRGEKWWDLPCKPHRQGPHWTTLPGPCDSPVAIRFFMSSNLTVQGLRVKNSPQFN 221
+G IDG+G+ WW++ W P + F S N+ + + K+SP +N
Sbjct: 140 NGTIDGQGDVWWNM----------WRQRTLQFTRPNLVEFVNSRNIIISNVIFKDSPFWN 289
Query: 222 FRFDGCIKVHVESIYITAPKLSPNTDGIHIENTNDVKIYNSMISNGDDCVSIGSG 276
C V V + I AP+ SPNTDG+ +++++V I +S IS GDD V++ SG
Sbjct: 290 IHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSSNVCIEDSYISTGDDLVAVKSG 454
>AV775483
Length = 503
Score = 65.5 bits (158), Expect = 2e-11
Identities = 44/140 (31%), Positives = 66/140 (46%)
Frame = -2
Query: 307 VSNITVRDSMMKGTDNGVRIKTWQGGSGSVSGVKFSNIRMVNVRNPIIIDQFYCITEGCT 366
V + V D GT N RIKT GGSG + F I + +PIIID +Y
Sbjct: 484 VEEVYVYDCSFTGTQNPARIKTLSGGSGYARKITFEKITLTKAGDPIIIDPYY-----AP 320
Query: 367 NKTSAVFVSNILYTNIKGTYDVRSLPMRFACSDSIPCTHLALSDIELLPAEGDIAHDPFC 426
S+V VS++ + I+GT + + CS C +L L I ++ + C
Sbjct: 319 EDDSSVPVSDVTFRGIRGT-SSDDIAINLHCSPP-GCFYLVLDQINIVSSVSGKKTSASC 146
Query: 427 WNAYGDLQTLTIPPVSCLMQ 446
NA+G + T ++P V CL++
Sbjct: 145 QNAHGRV-TSSVPSVPCLLR 89
>TC12747 weakly similar to UP|Q8LAH2 (Q8LAH2) Polygalacturonase-like
protein, partial (43%)
Length = 624
Score = 63.9 bits (154), Expect = 5e-11
Identities = 51/185 (27%), Positives = 79/185 (42%), Gaps = 13/185 (7%)
Frame = +2
Query: 163 GLIDGRGEKWWDLPCKPHRQGPHWTTLPGPCDSPVAIRFFMSSNLTVQGLRVKNSPQFNF 222
G IDG+G WWDL +T P I S N+T+ L + NSP +
Sbjct: 110 GTIDGQGSVWWDLI----------STGDLNYSRPHIIELIGSDNITISNLTILNSPSWGI 259
Query: 223 RFDGCIKVHVESIYITAPKLSPNTDGIHIENTNDVKIYNSMISNGDDCVSIGSG------ 276
C V + +I + AP SP+T GI +++ + I S IS G D +++ SG
Sbjct: 260 HPVYCRYVQIHNITVHAPPESPHTSGIVPDSSEYICIDKSNISTGHDAIALKSGWDEYGV 439
Query: 277 -----CYDVDIKNI--TCGPGHGISIGSLGIHNSRACVSNITVRDSMMKGTDNGVRIKTW 329
+V I ++ G G++ GS +SNI + + G+ +KT
Sbjct: 440 AYGKPTLNVHISSVYLQSSSGAGLAFGS----EMSGGISNIIAEQVHIINSKIGIELKTT 607
Query: 330 QGGSG 334
+G G
Sbjct: 608 KGRGG 622
>AV776486
Length = 409
Score = 60.8 bits (146), Expect = 4e-10
Identities = 35/88 (39%), Positives = 48/88 (53%), Gaps = 18/88 (20%)
Frame = +1
Query: 24 SAQARHHSHTKHNKHSHSHKS--------TKTSPP----------PPPHNCYNATGIFDV 65
+ + R+H H K NK S + KS + TSPP P P++ ++ +FDV
Sbjct: 142 NVEGRNHYHKKQNKKSPAPKSPAHSPAPASDTSPPSSTSPSVPSDPYPNDPGDSGCVFDV 321
Query: 66 TTFGAVGDGIADDTVSFKMAWDTACQSE 93
+FGAVGDG ADDT +F+ AW AC E
Sbjct: 322 MSFGAVGDGSADDTAAFREAWKAACAVE 405
>BP030183
Length = 483
Score = 60.5 bits (145), Expect = 6e-10
Identities = 32/72 (44%), Positives = 43/72 (59%), Gaps = 2/72 (2%)
Frame = +3
Query: 62 IFDVTTFGAVGDGIADDTVSFKMAWDTACQSELPGNVILVPKGFSFIIQSTIFTGPCQG- 120
I++V +FGA DG+ D T +F AW T C S PG ILVP G F++ S F GPC
Sbjct: 222 IYNVMSFGAKPDGVFDSTQAFMTAWQTVCHSPAPGR-ILVPAG-RFLVSSMFFQGPCLSP 395
Query: 121 -GIVFKVEGTLM 131
+ +VEGT++
Sbjct: 396 HPVTLQVEGTVL 431
>AV423118
Length = 472
Score = 54.7 bits (130), Expect = 3e-08
Identities = 35/151 (23%), Positives = 73/151 (48%), Gaps = 13/151 (8%)
Frame = +2
Query: 216 NSPQFNFRFDGCIKVHVESIYITAPKLSPNTDGIHIENTNDVKIYNSMISNGDDCVSIGS 275
N+P ++ C VH+ ++ I AP SP+T G+ ++++ V I + +I+ G D +++ S
Sbjct: 5 NAPAYSIHPVYCSYVHIHNLTIFAPPESPDTVGLVPDSSDHVCIEDCVIATGYDAIALKS 184
Query: 276 GCYDVDIK-------------NITCGPGHGISIGSLGIHNSRACVSNITVRDSMMKGTDN 322
G + I ++ G ++ GS + +SN+ V ++ + ++
Sbjct: 185 GWDEYGIAYGRPTENVHIRRVDLQASSGSALAFGS----DMSGGISNVLVENAHLHNSNG 352
Query: 323 GVRIKTWQGGSGSVSGVKFSNIRMVNVRNPI 353
G+ +T +G G + + S++ M NV I
Sbjct: 353 GIEFRTTRGRGGYMKDIIISDVEMKNVYTAI 445
>TC18673 similar to UP|Q9LWY0 (Q9LWY0) ESTs AU029388(E30287), partial (23%)
Length = 560
Score = 52.8 bits (125), Expect = 1e-07
Identities = 41/127 (32%), Positives = 58/127 (45%), Gaps = 12/127 (9%)
Frame = +2
Query: 245 NTDGIHIENTNDVKIYNSMISNGDDCVSIGSG------CYDVDIKNITC------GPGHG 292
NTDGI ++++DV I + IS GDD ++I SG Y NI G
Sbjct: 17 NTDGIDPDSSDDVCIEDCYISTGDDLIAIKSGWDEYGIAYGRPSTNIVIRRLVGKTQTSG 196
Query: 293 ISIGSLGIHNSRACVSNITVRDSMMKGTDNGVRIKTWQGGSGSVSGVKFSNIRMVNVRNP 352
I+IGS VS + D + N +RIKT +G G V + SN+ +VN+
Sbjct: 197 IAIGS----EMSGGVSEVHAEDIHFYDSYNAIRIKTSRGRGGYVRNIYISNMTLVNIEYA 364
Query: 353 IIIDQFY 359
I + Y
Sbjct: 365 ITFNGLY 385
>AU088950
Length = 423
Score = 47.4 bits (111), Expect = 5e-06
Identities = 26/108 (24%), Positives = 49/108 (45%)
Frame = +2
Query: 60 TGIFDVTTFGAVGDGIADDTVSFKMAWDTACQSELPGNVILVPKGFSFIIQSTIFTGPCQ 119
+G+ D++ FG + +D T +F AW+ AC S P +++ ++ + + GPC+
Sbjct: 107 SGVIDISKFGVKPN--SDITQAFTSAWNEACASPTPSKIVI--PSXTYXMGALELKGPCK 274
Query: 120 GGIVFKVEGTLMPPDGPESWPKNISRHQWLVFYRINGMSLEGSGLIDG 167
I +V+GT+ P QW+ + + G + DG
Sbjct: 275 APIEVQVDGTIQSSGNPSDL---XGAXQWVTVSYVPXLPYPGXSVFDG 409
>BP060510
Length = 462
Score = 45.4 bits (106), Expect = 2e-05
Identities = 31/89 (34%), Positives = 43/89 (47%), Gaps = 17/89 (19%)
Frame = +1
Query: 12 LAFCIFSLTLF----LSAQARHHSHTKHNKHSHSHKSTKTSPPPP------PHNCY---- 57
L I +LTLF ++ + R+H T S + P PP P + Y
Sbjct: 196 LILWIITLTLFNHNLVNVEGRYHHLTNQKNISPAPDDPPNDPSPPSISPSFPSDPYPNDP 375
Query: 58 ---NATGIFDVTTFGAVGDGIADDTVSFK 83
++ GIFDV + GAVGDG ADDT +F+
Sbjct: 376 QDSSSNGIFDVRSXGAVGDGSADDTPAFR 462
>AV780084
Length = 527
Score = 44.7 bits (104), Expect = 3e-05
Identities = 27/64 (42%), Positives = 37/64 (57%)
Frame = -3
Query: 368 KTSAVFVSNILYTNIKGTYDVRSLPMRFACSDSIPCTHLALSDIELLPAEGDIAHDPFCW 427
+TSAV V NI +T+I+GT ++FACSD+ PC L L +I L G +CW
Sbjct: 519 QTSAVQVRNISFTDIQGTSATEEA-IKFACSDASPCEGLYL*NIFLESCFGGNTRS-YCW 346
Query: 428 NAYG 431
A+G
Sbjct: 345 QAHG 334
>TC19633 similar to UP|Q7NET9 (Q7NET9) Glr3789 protein, partial (4%)
Length = 459
Score = 43.9 bits (102), Expect = 6e-05
Identities = 37/124 (29%), Positives = 57/124 (45%), Gaps = 2/124 (1%)
Frame = +3
Query: 47 TSPPPPPHNCYNATGIFDVTTFGAVGDGIADDTVSFKMAWDTACQSELPGNVILVPKGFS 106
TS PP Y V+ FGA GDG+ DT S + A + +C P VI G
Sbjct: 120 TSHPP-----YPIPLTVSVSDFGAAGDGVRYDTTSIQSAIN-SCPGSSPCRVIFPAPG-- 275
Query: 107 FIIQSTIFTGPCQGGIVFKVEGTLMPPDGP--ESWPKNISRHQWLVFYRINGMSLEGSGL 164
+ +T+F + G+V VE GP E +P+ SR ++ + ++G G+
Sbjct: 276 KYLTATVF---LKSGVVLFVEPGATILGGPRLEDYPREPSRWYVVLAENATDVGIDGGGV 446
Query: 165 IDGR 168
+DG+
Sbjct: 447 VDGQ 458
>BP042395
Length = 480
Score = 40.8 bits (94), Expect = 5e-04
Identities = 24/82 (29%), Positives = 38/82 (46%)
Frame = -1
Query: 363 EGCTNKTSAVFVSNILYTNIKGTYDVRSLPMRFACSDSIPCTHLALSDIELLPAEGDIAH 422
+GC + S V +S++ Y +I GT + ++F CS PC + L +++L D
Sbjct: 468 KGCPRQASGVKISDVTYKDIHGT-SATPVAIKFDCSSKYPCNGIKLQEVKLTYKSQDAQA 292
Query: 423 DPFCWNAYGDLQTLTIPPVSCL 444
N G T+ P SCL
Sbjct: 291 S---CNHAGGATMGTVQPESCL 235
>TC11359
Length = 544
Score = 36.6 bits (83), Expect = 0.009
Identities = 32/143 (22%), Positives = 52/143 (35%), Gaps = 26/143 (18%)
Frame = -3
Query: 328 TWQGGSGSVSGVKFSNIRMVNVRNPIIIDQFYCITEGCTN-------------------- 367
T +GG G + + NI + P+ IDQ Y T N
Sbjct: 443 TMKGGKGYAKAIIYENIIVNQTNYPVYIDQHYMRTPEKVNILSYCLLFVDLFGDNLHHSF 264
Query: 368 ------KTSAVFVSNILYTNIKGTYDVRSLPMRFACSDSIPCTHLALSDIELLPAEGDIA 421
+ A+ V+++ + NI GT + C+ I C ++ L I + + D
Sbjct: 263 PLLFWLQKEALKVTDVTFRNIHGTC-TNEHAVVLDCA-KIGCDNIKLQQINITSIDLDKP 90
Query: 422 HDPFCWNAYGDLQTLTIPPVSCL 444
C N +G + P V+CL
Sbjct: 89 ASAICHNVHGTATDVISPRVTCL 21
>BP034892
Length = 483
Score = 33.5 bits (75), Expect = 0.076
Identities = 22/81 (27%), Positives = 33/81 (40%)
Frame = +1
Query: 19 LTLFLSAQARHHSHTKHNKHSHSHKSTKTSPPPPPHNCYNATGIFDVTTFGAVGDGIADD 78
L F ++ RH S + S SP P P N ++ VT++GA G +D
Sbjct: 145 LQSFKASLTRHDSIASAPSSFSASSSPSPSPSPQPFQGVNNPRVYHVTSYGADPSGNSDS 324
Query: 79 TVSFKMAWDTACQSELPGNVI 99
T + A A + G +I
Sbjct: 325 TEALLAAIADAAKGPSEGRLI 387
>TC11304 similar to PIR|T04669|T04669 serine O-acetyltransferase F8D20.150
- Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (24%)
Length = 775
Score = 33.5 bits (75), Expect = 0.076
Identities = 16/43 (37%), Positives = 23/43 (53%)
Frame = +3
Query: 27 ARHHSHTKHNKHSHSHKSTKTSPPPPPHNCYNATGIFDVTTFG 69
A+H K N H +T T PPPPP + +N+ F+ T+ G
Sbjct: 213 AKHRGQCKKNHH---FATTTTPPPPPPPHHHNSLPYFNPTSHG 332
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.138 0.449
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,580,772
Number of Sequences: 28460
Number of extensions: 189262
Number of successful extensions: 2511
Number of sequences better than 10.0: 145
Number of HSP's better than 10.0 without gapping: 2150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2379
length of query: 460
length of database: 4,897,600
effective HSP length: 93
effective length of query: 367
effective length of database: 2,250,820
effective search space: 826050940
effective search space used: 826050940
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0122.9