BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0118a.5
(351 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV780445 83 1e-30
BP068806 91 3e-19
BP076564 91 4e-19
BE122611 53 5e-11
AV411435 44 5e-05
AV428268 40 8e-04
AU089037 37 0.006
AV412895 36 0.008
AV426428 36 0.011
TC10037 34 0.032
TC10886 similar to UP|Q8GSM8 (Q8GSM8) Squalene monooxygenase 1 ... 33 0.072
AV418280 32 0.16
TC9544 UP|AAO92441 (AAO92441) Homoglutathione synthetase, complete 28 2.3
AV427022 28 3.0
TC11605 28 3.0
TC12100 similar to UP|DER7_MOUSE (Q60774) Dermal papilla derived... 28 3.0
TC11191 similar to UP|Q9LQZ3 (Q9LQZ3) F10A5.28, partial (9%) 27 3.9
TC13043 similar to GB|BAB21544.1|12641847|AB022782 hydroxyprolin... 27 3.9
TC12426 27 6.7
AV769907 27 6.7
>AV780445
Length = 504
Score = 83.2 bits (204), Expect(2) = 1e-30
Identities = 35/71 (49%), Positives = 47/71 (65%)
Frame = +1
Query: 122 RSNHAISFLSGIWSVWLWRNNMCFEETPWNLAEAWRRLSHVHDEMLQTSQDWSPGDLNSL 181
R +++ L+G+W VW WRNNM FE++PW++ EAWR+L H HDE + T D GD S
Sbjct: 202 RIRNSVKCLAGVW*VWKWRNNMVFEDSPWSVDEAWRKLGHEHDEFI-TFSDGDAGDPGSW 378
Query: 182 LCVRWHPPARG 192
L RW PP +G
Sbjct: 379 LSSRWQPPRQG 411
Score = 66.6 bits (161), Expect(2) = 1e-30
Identities = 28/46 (60%), Positives = 33/46 (70%)
Frame = +2
Query: 75 RCSAAEETILHCLRDCPHSRELWLRLGATTWRSFATMDVEAWITSL 120
RC A EE I HCLRDCPHS+E+WLRLGA W + D +WI+SL
Sbjct: 65 RCPAPEEDI*HCLRDCPHSQEIWLRLGAWAWTNLGVGDHRSWISSL 202
>BP068806
Length = 418
Score = 90.9 bits (224), Expect = 3e-19
Identities = 41/88 (46%), Positives = 56/88 (63%)
Frame = +3
Query: 39 KVPEKM*LFVWLILNKALQTNGNRFRCHLAQSPSRSRCSAAEETILHCLRDCPHSRELWL 98
+VPEK+ ++VW I+ A+Q N RFRC LA SP+ RC E I HCLRDC +R++W+
Sbjct: 147 EVPEKIRVWVWFIMQGAIQVNSFRFRCKLATSPAGPRCQHQFEDIWHCLRDCRIARDVWV 326
Query: 99 RLGATTWRSFATMDVEAWITSLARSNHA 126
RL A W F + +AW+ S A+ HA
Sbjct: 327 RLNALGWPGFFVSNAQAWVKSQAQGPHA 410
>BP076564
Length = 371
Score = 90.5 bits (223), Expect = 4e-19
Identities = 44/105 (41%), Positives = 63/105 (59%)
Frame = +2
Query: 38 LKVPEKM*LFVWLILNKALQTNGNRFRCHLAQSPSRSRCSAAEETILHCLRDCPHSRELW 97
LKV EK V L L++AL N R C +A + RCSAA + ILHCL+DCPHSRE+W
Sbjct: 56 LKVVEKCKFMVGLGLHQALPINARRHACSMAATGGCGRCSAAVKDILHCLQDCPHSREVW 235
Query: 98 LRLGATTWRSFATMDVEAWITSLARSNHAISFLSGIWSVWLWRNN 142
RLG +++ F + D +W+ S+ + + + +W +W RNN
Sbjct: 236 NRLGCSSFGGFLSSDGWSWLLSIMKQPNVEKLIYILWWIWR*RNN 370
>BE122611
Length = 357
Score = 53.1 bits (126), Expect(2) = 5e-11
Identities = 23/62 (37%), Positives = 32/62 (51%)
Frame = -3
Query: 1 IWGPSPTGCYTAREAYGWLNNLDHEGQNGRCWQWVWKLKVPEKM*LFVWLILNKALQTNG 60
+W GCY+A+ Y L ++ G R W VWK PEK+ F+WL+ AL +N
Sbjct: 322 LWTEESNGCYSAKSTYNLL--IEDGGNESRFWSLVWKANAPEKVRFFLWLVGRNALPSNA 149
Query: 61 NR 62
R
Sbjct: 148 RR 143
Score = 30.4 bits (67), Expect(2) = 5e-11
Identities = 14/32 (43%), Positives = 16/32 (49%)
Frame = -2
Query: 66 HLAQSPSRSRCSAAEETILHCLRDCPHSRELW 97
HLA S + RC A E H R CP S +W
Sbjct: 134 HLASSAACIRCGAPSEDANHVFRWCPGSAWMW 39
>AV411435
Length = 426
Score = 43.5 bits (101), Expect = 5e-05
Identities = 27/105 (25%), Positives = 45/105 (42%), Gaps = 9/105 (8%)
Frame = +3
Query: 2 WGPSPTGCYTAREAYGW-------LNNLDHEGQNGRCWQW--VWKLKVPEKM*LFVWLIL 52
W P+G Y+ + Y L+NL + + W +W +VP+K+ F+W
Sbjct: 51 WPFIPSGEYSVKSGYRAVKATQFNLSNLASSSSHPSSFLWHTIWGAQVPKKVRSFLWRAA 230
Query: 53 NKALQTNGNRFRCHLAQSPSRSRCSAAEETILHCLRDCPHSRELW 97
N A+ N R ++ + C+ E I H L C +R +W
Sbjct: 231 NNAIPVKRNLKRRNMGRDDFCPICNKGPEDINHALLACEWTRAVW 365
>AV428268
Length = 429
Score = 39.7 bits (91), Expect = 8e-04
Identities = 25/91 (27%), Positives = 41/91 (44%)
Frame = +3
Query: 10 YTAREAYGWLNNLDHEGQNGRCWQWVWKLKVPEKM*LFVWLILNKALQTNGNRFRCHLAQ 69
Y+ + AY +L+ + Q ++ +W +KVP W +L +QT N R +
Sbjct: 6 YSVKSAYDFLHGCAN-AQWDNIFKLLWSVKVPSNAIALSWKVLINRVQTKVNLNRRGVVI 182
Query: 70 SPSRSRCSAAEETILHCLRDCPHSRELWLRL 100
S CS EE+ H L CP +W ++
Sbjct: 183 SNVCPLCSLDEESTDHLLFSCPIV*RIWSKI 275
>AU089037
Length = 245
Score = 36.6 bits (83), Expect = 0.006
Identities = 19/63 (30%), Positives = 28/63 (44%), Gaps = 3/63 (4%)
Frame = -1
Query: 32 WQWVWKLKVPEKM*LFVWLILNKALQTNGNRFRCHLAQSPSRS---RCSAAEETILHCLR 88
+Q +WK+ P F W ++ +QT GN R + Q + CS EE+ H
Sbjct: 200 FQKLWKVPAPSNAVSFAWRLILDRVQTRGNLRRRQVIQQSEEALCPMCSQCEESSSHLFF 21
Query: 89 DCP 91
CP
Sbjct: 20 SCP 12
>AV412895
Length = 405
Score = 36.2 bits (82), Expect = 0.008
Identities = 24/82 (29%), Positives = 33/82 (39%), Gaps = 3/82 (3%)
Frame = +1
Query: 35 VWKLKVPEKM*LFVWLILNKALQTNGNRFR---CHLAQSPSRSRCSAAEETILHCLRDCP 91
+W + P + FVW +L +QT N + H A C AEE+ H L C
Sbjct: 82 IWMVPAPLNIKAFVWRVLLGRIQTRDNLLKRQVIHNALDAICPLCGLAEESGSHLLFSCA 261
Query: 92 HSRELWLRLGATTWRSFATMDV 113
S +W A W +T V
Sbjct: 262 ESMLIWYECFA--WLGVSTAQV 321
>AV426428
Length = 413
Score = 35.8 bits (81), Expect = 0.011
Identities = 23/84 (27%), Positives = 38/84 (44%), Gaps = 2/84 (2%)
Frame = +3
Query: 67 LAQSPSRSRCSAAEETILHCLRDCPHSRELWL--RLGATTWRSFATMDVEAWITSLARSN 124
LA+ P RC AA +T H + CP +W LG + + A +A +
Sbjct: 150 LAEDPICPRCLAAPKTADHAVLFCP*VHPIWFASSLGFRLNQECKMHEFVADFMQVAD*D 329
Query: 125 HAISFLSGIWSVWLWRNNMCFEET 148
FL+ ++++W +N +CF T
Sbjct: 330 TCGEFLTILYAIWTAQNELCFRYT 401
>TC10037
Length = 560
Score = 34.3 bits (77), Expect = 0.032
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = -3
Query: 75 RCSAAEETILHCLRDCPHSRELW 97
RC EET+ H LRDCP +R +W
Sbjct: 381 RCDRLEETMDHALRDCP*ARRVW 313
>TC10886 similar to UP|Q8GSM8 (Q8GSM8) Squalene monooxygenase 1 , partial
(27%)
Length = 1135
Score = 33.1 bits (74), Expect = 0.072
Identities = 11/24 (45%), Positives = 18/24 (74%)
Frame = -1
Query: 77 SAAEETILHCLRDCPHSRELWLRL 100
S + E +LH LR+ PH+ E+W+R+
Sbjct: 97 STSREDVLHSLREFPHAMEMWMRM 26
Score = 29.3 bits (64), Expect = 1.0
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = -3
Query: 8 GCYTAREAYGWLNNLD 23
GCY RE Y W N+LD
Sbjct: 161 GCYMVREFYSWFNSLD 114
>AV418280
Length = 398
Score = 32.0 bits (71), Expect = 0.16
Identities = 21/80 (26%), Positives = 36/80 (44%), Gaps = 10/80 (12%)
Frame = -2
Query: 76 CSAAEETILHCLRDCPHSRELW----------LRLGATTWRSFATMDVEAWITSLARSNH 125
C+ EE H C S +W + L A+T +FA + A +
Sbjct: 379 CTTVEECSGHLFFTCVFSMGVWQALHRWLGISVALPASTLANFAQFSITARNKNQRLGEL 200
Query: 126 AISFLSGIWSVWLWRNNMCF 145
AI +++ +WS+W+ RN++ F
Sbjct: 199 AI-WIATVWSLWIQRNSIIF 143
>TC9544 UP|AAO92441 (AAO92441) Homoglutathione synthetase, complete
Length = 1860
Score = 28.1 bits (61), Expect = 2.3
Identities = 9/14 (64%), Positives = 10/14 (71%)
Frame = +2
Query: 25 EGQNGRCWQWVWKL 38
EGQNG W W+W L
Sbjct: 2 EGQNGGRWMWIWLL 43
>AV427022
Length = 429
Score = 27.7 bits (60), Expect = 3.0
Identities = 33/129 (25%), Positives = 47/129 (35%), Gaps = 20/129 (15%)
Frame = +1
Query: 76 CSAAEETILHCLRDCPHSRELWLRLGATTWRSFATMDVE------------AWITSLARS 123
C AEE+ H L C +S + A W +T V W +
Sbjct: 49 CCLAEESGSHLLFSCSNSMLI*YECHA--WLGVSTAQVSDPKVHLLQFSSIGWSKAQKLG 222
Query: 124 NHAISFLSGIWSVWLWRNNMCF---EETPWNLAE-----AWRRLSHVHDEMLQTSQDWSP 175
AI ++S +WS+W RN + F E L E AWR L D+ + +W
Sbjct: 223 ESAI-WMSVLWSIWCLRNMVVFNGGELDKDQLLEQIQVTAWRWLRSRLDDF*YSWYEWKS 399
Query: 176 GDLNSLLCV 184
LL +
Sbjct: 400 NPRICLLSI 426
>TC11605
Length = 546
Score = 27.7 bits (60), Expect = 3.0
Identities = 12/47 (25%), Positives = 25/47 (52%), Gaps = 3/47 (6%)
Frame = +1
Query: 113 VEAWITSLARSNHAISFL---SGIWSVWLWRNNMCFEETPWNLAEAW 156
++ W + A+S+ + L + +WS+WL RN++ F + + W
Sbjct: 73 LKEWPSLRAKSDRVLWELVPCAVVWSIWLARNDLVFNQKEFIYENVW 213
>TC12100 similar to UP|DER7_MOUSE (Q60774) Dermal papilla derived protein 7
homolog (19.5), partial (7%)
Length = 573
Score = 27.7 bits (60), Expect = 3.0
Identities = 13/40 (32%), Positives = 18/40 (44%), Gaps = 6/40 (15%)
Frame = +2
Query: 129 FLSGIWSVW-----LWRNNMCFEETPWNLAEAWR-RLSHV 162
F+ G+W +W WRN F WN + RL H+
Sbjct: 386 FVVGVWHIWCSVVRYWRNPKTFRVRVWNPVPGFNGRLKHL 505
>TC11191 similar to UP|Q9LQZ3 (Q9LQZ3) F10A5.28, partial (9%)
Length = 544
Score = 27.3 bits (59), Expect = 3.9
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = -2
Query: 122 RSNHAISFLSGIWSVWLWRN 141
+SNH +++ S IW+ WLW N
Sbjct: 537 KSNHNLAYKS-IWNFWLWPN 481
>TC13043 similar to GB|BAB21544.1|12641847|AB022782 hydroxyproline-rich
glycoprotein {Arabidopsis thaliana;} , partial (6%)
Length = 678
Score = 27.3 bits (59), Expect = 3.9
Identities = 10/21 (47%), Positives = 15/21 (70%)
Frame = +3
Query: 127 ISFLSGIWSVWLWRNNMCFEE 147
+ +L+ IW+VW RNN+ F E
Sbjct: 588 VVWLAVIWTVWNGRNNLVFRE 650
>TC12426
Length = 539
Score = 26.6 bits (57), Expect = 6.7
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = -2
Query: 83 ILHCLRDCPHSRELWL 98
+LHCLR H++ELW+
Sbjct: 514 LLHCLR*RKHAKELWI 467
>AV769907
Length = 352
Score = 26.6 bits (57), Expect = 6.7
Identities = 12/19 (63%), Positives = 15/19 (78%), Gaps = 1/19 (5%)
Frame = +3
Query: 56 LQTNGNRFRCHL-AQSPSR 73
L T+ NRFRCHL +SPS+
Sbjct: 225 LTTHNNRFRCHLPPRSPSK 281
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.342 0.147 0.572
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,121,443
Number of Sequences: 28460
Number of extensions: 162013
Number of successful extensions: 1992
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 1950
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1987
length of query: 351
length of database: 4,897,600
effective HSP length: 91
effective length of query: 260
effective length of database: 2,307,740
effective search space: 600012400
effective search space used: 600012400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.5 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0118a.5