Lotus
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0115.1
         (204 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC8460 similar to GB|AAN73294.1|25141199|BT002297 At5g12010/F14F...    29  0.50
NP840539 hypothetical protein [Lotus corniculatus var. japonicus]      27  1.9
NP566648 hypothetical protein [Lotus japonicus]                        27  1.9
TC12207 similar to PIR|T06789|T06789 hydroxyproline-rich glycopr...    27  2.5
BP039178                                                               27  3.2
TC15520 similar to UP|Q8L5E1 (Q8L5E1) Acid phosphatase  , partia...    25  7.2
TC8361 homologue to UP|Q9ST36 (Q9ST36) JPR ORF1 protein, partial...    25  9.4

>TC8460 similar to GB|AAN73294.1|25141199|BT002297 At5g12010/F14F18_180
           {Arabidopsis thaliana;}, partial (36%)
          Length = 880

 Score = 29.3 bits (64), Expect = 0.50
 Identities = 23/103 (22%), Positives = 43/103 (41%)
 Frame = +3

Query: 93  NEPTYDDAMFRRRYRMQKHLFLRIVDDLSSSDNYFTQRVDAANKQGISPLAKCTTAMRML 152
           + P + +  FRR +RM K  F  I  +L S+      + +   ++ I    +    +  L
Sbjct: 357 SHPDFPEEEFRRSFRMSKATFEMICRELDSA----VTKKNTMLREAIPVRQRVAVCIWRL 524

Query: 153 AYGVAADAVDEYIKIGGTTALECLRRFCKGIIQLYEQQYLRAP 195
           A G     V +   +G +T  + +   C  I  +   ++LR P
Sbjct: 525 ATGDPLRLVAKRFGLGISTCHKLVLEVCSAIKTVLMPKFLRWP 653


>NP840539 hypothetical protein [Lotus corniculatus var. japonicus]
          Length = 3654

 Score = 27.3 bits (59), Expect = 1.9
 Identities = 17/42 (40%), Positives = 25/42 (59%), Gaps = 1/42 (2%)
 Frame = +2

Query: 37  DLAAYIQNSQIEEAYVLNRF-RERRRKIREDTAPRSRKYLGR 77
           DLA + ++ ++EE YVL RF R  ++K+ E  A  S   L R
Sbjct: 749 DLAIFQKHLEVEELYVLERF*RLMKKKL*EKCARSSYLLLTR 874


>NP566648 hypothetical protein [Lotus japonicus]
          Length = 1101

 Score = 27.3 bits (59), Expect = 1.9
 Identities = 17/42 (40%), Positives = 25/42 (59%), Gaps = 1/42 (2%)
 Frame = +2

Query: 37  DLAAYIQNSQIEEAYVLNRF-RERRRKIREDTAPRSRKYLGR 77
           DLA + ++ ++EE YVL RF R  ++K+ E  A  S   L R
Sbjct: 581 DLAIFQKHLEVEELYVLERF*RLMKKKL*EKCARSSYLLLTR 706


>TC12207 similar to PIR|T06789|T06789 hydroxyproline-rich glycoprotein 1 -
           garden pea (fragment) {Pisum sativum;}, partial (51%)
          Length = 638

 Score = 26.9 bits (58), Expect = 2.5
 Identities = 12/26 (46%), Positives = 17/26 (65%)
 Frame = -1

Query: 16  SQISSNLLQISQKMDPSECPFDLAAY 41
           S +SS LLQ+S+ +  S CP DL  +
Sbjct: 461 SCLSSTLLQLSKLLPYSSCPLDLCQH 384


>BP039178 
          Length = 589

 Score = 26.6 bits (57), Expect = 3.2
 Identities = 12/21 (57%), Positives = 15/21 (71%)
 Frame = -1

Query: 52  VLNRFRERRRKIREDTAPRSR 72
           +LNR RER+RKIR    P S+
Sbjct: 103 ILNRERERKRKIRAGQRPWSK 41


>TC15520 similar to UP|Q8L5E1 (Q8L5E1) Acid phosphatase  , partial (17%)
          Length = 474

 Score = 25.4 bits (54), Expect = 7.2
 Identities = 9/20 (45%), Positives = 15/20 (75%)
 Frame = +2

Query: 103 RRRYRMQKHLFLRIVDDLSS 122
           RRR ++ KHL L ++D++ S
Sbjct: 182 RRRRKLNKHLLLTVIDEVVS 241


>TC8361 homologue to UP|Q9ST36 (Q9ST36) JPR ORF1 protein, partial (92%)
          Length = 1523

 Score = 25.0 bits (53), Expect = 9.4
 Identities = 12/30 (40%), Positives = 15/30 (50%)
 Frame = +3

Query: 108  MQKHLFLRIVDDLSSSDNYFTQRVDAANKQ 137
            M+KH F  I D   +S  YFT   D   +Q
Sbjct: 1149 MKKHNFTSIEDFRGTSLQYFTTHTDLVQRQ 1238


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.324    0.137    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,995,342
Number of Sequences: 28460
Number of extensions: 31720
Number of successful extensions: 194
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 194
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 194
length of query: 204
length of database: 4,897,600
effective HSP length: 86
effective length of query: 118
effective length of database: 2,450,040
effective search space: 289104720
effective search space used: 289104720
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 53 (25.0 bits)


Lotus: description of TM0115.1