BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0114.12
(1414 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG662087 199 3e-51
TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase regi... 152 5e-37
BP030435 119 3e-27
BI417841 82 4e-16
TC18698 79 4e-15
BF177840 66 4e-11
TC16320 weakly similar to UP|Q84TE0 (Q84TE0) At5g51080, partial ... 62 8e-10
AV427422 59 5e-09
AU089582 52 3e-08
BP055130 55 1e-07
AV410603 53 3e-07
BP054462 42 9e-04
BG662122 40 0.002
BP046863 29 4.5
TC12003 homologue to UP|AAQ55798 (AAQ55798) Actin, partial (50%) 29 5.9
TC8037 similar to UP|AAR14273 (AAR14273) Predicted protein, part... 29 5.9
BP041751 28 7.7
TC16027 28 7.7
>BG662087
Length = 373
Score = 199 bits (505), Expect = 3e-51
Identities = 91/120 (75%), Positives = 105/120 (86%)
Frame = +1
Query: 465 NGKWRMCTDYTSLNKVCPKDSYPLPNVDKLVDGASGNELLSLMDAYSGYNQIMMHPSDEE 524
+GKWRM DYT LNK CPKDSYPLP++DKLVDGAS NELLSLMDAYSGY+QI MHPSDE+
Sbjct: 13 SGKWRMWVDYTDLNKACPKDSYPLPSIDKLVDGASDNELLSLMDAYSGYHQIKMHPSDED 192
Query: 525 STAFMTNQANYCYKTMPFGLKNAGATYQRLMDKIFSKQVGRNMEVYVDDMIVKSARASDH 584
TAFMT + NYCY+T+PFGLKNAGATYQ LMD++F VGRNMEVY+D+MIVKSA ++H
Sbjct: 193 KTAFMTARVNYCYQTIPFGLKNAGATYQXLMDRVFXDXVGRNMEVYLDNMIVKSALRANH 372
>TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase region
(Fragment), partial (10%)
Length = 572
Score = 152 bits (383), Expect = 5e-37
Identities = 74/124 (59%), Positives = 91/124 (72%)
Frame = +2
Query: 1201 DNGTQFTSSVTREFCAEMGIEMRFASVEHPQTNGQAESANKVILKGLKKKLDEAKGLWAE 1260
+NG QFTS T++FC MGI+MRF+SV+HPQTNGQ E+ANKVILKG+K++L EA+G W +
Sbjct: 8 ENGIQFTSKQTQDFCDGMGIQMRFSSVKHPQTNGQTEAANKVILKGIKRRLYEAEGRWID 187
Query: 1261 ELPGVLWAYNTTEQSSTKETPYRLTYGTDAMLSVEIENQSWRVARFNENDNGENLIANLI 1320
ELP VLW+YNT QSS KETP+ LTYG D ML VEI N SW +E +N N+
Sbjct: 188 ELPIVLWSYNTMPQSSIKETPF*LTYGADTMLPVEISNFSW*TKARHEEENRMNMAVGWE 367
Query: 1321 MLPE 1324
LPE
Sbjct: 368 KLPE 379
>BP030435
Length = 533
Score = 119 bits (298), Expect = 3e-27
Identities = 72/169 (42%), Positives = 95/169 (55%)
Frame = +1
Query: 48 CEYHKALGHTTDNCWNLRREIDRLIKAGHLANFVKDTATPEVAKITQEDKGKGKEIVEEL 107
C YH+A+GH T+ C L+RE+ +LI G K T+ G
Sbjct: 103 CVYHQAMGHITEECRTLQREVGKLIATG---------------KPTRIQGGNAPV----- 222
Query: 108 GDPVGECSSIAGGFGGGAISSRARKRYVATVHSVQESNEGECWVNHSPIIFTPQDFAHVI 167
P G +IAGGFGGG I+S ARKRY V++V E G +H I F+ DF +
Sbjct: 223 --PKG-VHTIAGGFGGGGITSAARKRYARAVNTVTEVLFG---FSHPVITFSSDDFIRIK 384
Query: 168 PYDNDPIVVTIRVNNYVTKKVFLDQGSSADIIYGDAFDRLGLKESNLKP 216
P+ ++PIV+ +RVN ++V LDQGSSADIIYG FDRLGL E++L P
Sbjct: 385 PHLDNPIVILLRVNQLNVQRVLLDQGSSADIIYGGVFDRLGLNEADLTP 531
>BI417841
Length = 617
Score = 82.4 bits (202), Expect = 4e-16
Identities = 47/125 (37%), Positives = 70/125 (55%), Gaps = 4/125 (3%)
Frame = +1
Query: 847 LSVDGSSN----NTGSGAGITIESPDKMIIEQSLKFEFKASNNQSEYEALIAGLRLAIEL 902
L DGSS + G+GA + E K+ + + + + +NNQ+EY LI GL+ A E
Sbjct: 142 LEFDGSSKGNPGSAGAGAVLRAEDGSKVYLREGVGNQ---TNNQAEYRGLILGLKHAHEQ 312
Query: 903 GVQKLFIKGDSQLVVKQVKGEYQVKDPQLSKYLEVVRRLMMEVKEIKIEHVPRGQNERAD 962
G Q + +KGDSQLV KQV+G ++ ++P ++ + L + + I HVPR N AD
Sbjct: 313 GYQHINVKGDSQLVCKQVEGSWKARNPNIASLCNEAKELKSKFQSFDINHVPRQYNSEAD 492
Query: 963 VLAKL 967
V A L
Sbjct: 493 VQANL 507
>TC18698
Length = 808
Score = 79.3 bits (194), Expect = 4e-15
Identities = 38/66 (57%), Positives = 48/66 (72%)
Frame = -2
Query: 523 EESTAFMTNQANYCYKTMPFGLKNAGATYQRLMDKIFSKQVGRNMEVYVDDMIVKSARAS 582
++ T N+ NY Y+ MP GLKN TYQRLMDKIF KQ+ +N+EVYV+DMIVKS++
Sbjct: 807 KKKTTLKINRVNYYYQVMPLGLKNI*TTYQRLMDKIFHKQI*KNVEVYVEDMIVKSSQE* 628
Query: 583 DHGGDL 588
H GDL
Sbjct: 627 FHRGDL 610
>BF177840
Length = 410
Score = 65.9 bits (159), Expect = 4e-11
Identities = 30/100 (30%), Positives = 56/100 (56%)
Frame = +2
Query: 1197 ALVMDNGTQFTSSVTREFCAEMGIEMRFASVEHPQTNGQAESANKVILKGLKKKLDEAKG 1256
++V D T+F S R ++G ++ +++ HPQT+GQ E NK + L+ L+
Sbjct: 14 SIVSDRDTKFISHFWRTLWGKVGTKLLYSTTCHPQTDGQTEVVNKTLSTLLRSVLERNLK 193
Query: 1257 LWAEELPGVLWAYNTTEQSSTKETPYRLTYGTDAMLSVEI 1296
+W LP + +AYN S+TK +P+ + YG + + +++
Sbjct: 194 MWETWLPHIEFAYNRVVHSTTKHSPFEIVYGYNPLTPLDL 313
>TC16320 weakly similar to UP|Q84TE0 (Q84TE0) At5g51080, partial (11%)
Length = 632
Score = 61.6 bits (148), Expect = 8e-10
Identities = 32/91 (35%), Positives = 49/91 (53%)
Frame = +2
Query: 889 YEALIAGLRLAIELGVQKLFIKGDSQLVVKQVKGEYQVKDPQLSKYLEVVRRLMMEVKEI 948
Y LI GL+ AI+ G + + +KGDS LV QV+G +++K+ ++ + L +
Sbjct: 2 YRGLILGLKHAIKEGYKHIQVKGDSMLVCNQVQGLWKIKNQNIASLCSEAKELKNKFLSF 181
Query: 949 KIEHVPRGQNERADVLAKLASTGRLGNYQTV 979
KI H+PR N ADV A + R G + V
Sbjct: 182 KINHIPREYNSEADVQANFGISLRAGQVEEV 274
>AV427422
Length = 417
Score = 58.9 bits (141), Expect = 5e-09
Identities = 36/127 (28%), Positives = 65/127 (50%), Gaps = 1/127 (0%)
Frame = +1
Query: 1156 IIVAVDYFTKWIEAEAVATITAAKVRNFLWQRIVCRF-GVPMALVMDNGTQFTSSVTREF 1214
++V VD +K+ + AKV ++ R V R GVP+++V D F S+ +E
Sbjct: 37 VLVVVDRLSKFSHFVPLKHPYTAKVIADIFVREVVRLHGVPLSIVSDRDPLFMSNFWKEL 216
Query: 1215 CAEMGIEMRFASVEHPQTNGQAESANKVILKGLKKKLDEAKGLWAEELPGVLWAYNTTEQ 1274
G +++ ++ HP+++GQ E N+ + L+ + + WA +P + YNT+
Sbjct: 217 FKMQGTKLKMSTAYHPESDGQTEVVNRCLETYLRCFIADQPKSWAHWVPWAEYWYNTSYH 396
Query: 1275 SSTKETP 1281
ST +TP
Sbjct: 397 VSTGQTP 417
>AU089582
Length = 383
Score = 51.6 bits (122), Expect(2) = 3e-08
Identities = 24/80 (30%), Positives = 44/80 (55%)
Frame = +2
Query: 1177 AAKVRNFLWQRIVCRFGVPMALVMDNGTQFTSSVTREFCAEMGIEMRFASVEHPQTNGQA 1236
A++ IV GVP++++ D G QFTS R F +G ++ ++ HPQT+GQ+
Sbjct: 11 ASQYAKIYLDEIVSLHGVPVSIISDRGAQFTSHFWRSFQTALGTRLKMSTAFHPQTDGQS 190
Query: 1237 ESANKVILKGLKKKLDEAKG 1256
E +++ L+ + + +G
Sbjct: 191 ERTIQILEDMLRACVXDLRG 250
Score = 24.3 bits (51), Expect(2) = 3e-08
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = +3
Query: 1255 KGLWAEELPGVLWAYNTTEQSSTKETPYRLTYG 1287
+G W + L + +AYN + +SS P+ YG
Sbjct: 246 EGSWDQYLSLMEFAYNNSYRSSI*MAPFEALYG 344
>BP055130
Length = 567
Score = 54.7 bits (130), Expect = 1e-07
Identities = 37/108 (34%), Positives = 53/108 (48%), Gaps = 1/108 (0%)
Frame = +2
Query: 1156 IIVAVDYFTKWIE-AEAVATITAAKVRNFLWQRIVCRFGVPMALVMDNGTQFTSSVTREF 1214
IIV VD +K+ A A T+++V IV G+P A+V D FTS+ + F
Sbjct: 191 IIVVVDRLSKYGHFAPHRANYTSSQVAETFVSTIVKLHGMPRAIVSDRDKAFTSAFWKHF 370
Query: 1215 CAEMGIEMRFASVEHPQTNGQAESANKVILKGLKKKLDEAKGLWAEEL 1262
G + +S HPQT+GQ E+ NK + L+ + E LW L
Sbjct: 371 FKLHGTTLNMSSSYHPQTDGQTEALNKCLELYLRCFVHETPRLWVSYL 514
>AV410603
Length = 162
Score = 53.1 bits (126), Expect = 3e-07
Identities = 22/49 (44%), Positives = 33/49 (66%)
Frame = +1
Query: 483 KDSYPLPNVDKLVDGASGNELLSLMDAYSGYNQIMMHPSDEESTAFMTN 531
KDS+P+P VD+L+D G++ S +D SGY+QI++ P D T F T+
Sbjct: 10 KDSFPMPTVDELLDELRGSQFFSKLDLRSGYHQILVKPEDRHKTVFRTH 156
>BP054462
Length = 422
Score = 41.6 bits (96), Expect = 9e-04
Identities = 37/149 (24%), Positives = 68/149 (44%), Gaps = 7/149 (4%)
Frame = +1
Query: 1270 NTTEQSSTKETPYRLTYGTDAMLSVEIENQSWRVARFNENDNGEN-----LIANLIMLPE 1324
N+ S K +P++ YG + + ++ ++A N+ G + L ANL+ +
Sbjct: 1 NSNYNRSAKMSPFQALYGREPPVLLQGTTIPSKIAAVNDLQVGRDELLSDLRANLLKSQD 180
Query: 1325 EQRE-AHIRNEAGKVKVARKFSTKVVPRKMRVGDLVL*KNTIPDKHN-KLSPNWGGPYRI 1382
R A+ + ++ + K+ P + R ++ K N KLSP + GPY I
Sbjct: 181 MMRTYANKKRRDVDYQIGDEVFLKLQPYRRR---------SLAKKMNEKLSPRYYGPYPI 333
Query: 1383 IGDVGGEAYKLEQLSGQKVPRTWNASHLK 1411
+ +G AY+LE + +V ++ S LK
Sbjct: 334 VAKIGAVAYRLELPAHSRVHPVFHVSLLK 420
>BG662122
Length = 386
Score = 40.4 bits (93), Expect = 0.002
Identities = 15/33 (45%), Positives = 24/33 (72%)
Frame = +2
Query: 48 CEYHKALGHTTDNCWNLRREIDRLIKAGHLANF 80
CEY +++ H D+C+ L+REI++LIK G L +
Sbjct: 212 CEYRRSVVHDIDDCFTLKREIEKLIKMGRLKQY 310
>BP046863
Length = 580
Score = 29.3 bits (64), Expect = 4.5
Identities = 16/51 (31%), Positives = 29/51 (56%)
Frame = +1
Query: 1362 KNTIPDKHNKLSPNWGGPYRIIGDVGGEAYKLEQLSGQKVPRTWNASHLKQ 1412
+N K+ KLSP + GP++++ V AY L+ S +V ++ S L++
Sbjct: 427 QNLAQRKNQKLSPRFYGPFKVLERVVQVAY*LDLXSESRVHPVFHLSLLEK 579
>TC12003 homologue to UP|AAQ55798 (AAQ55798) Actin, partial (50%)
Length = 612
Score = 28.9 bits (63), Expect = 5.9
Identities = 9/19 (47%), Positives = 11/19 (57%)
Frame = +3
Query: 35 WGMLIYCRRIWRWCEYHKA 53
W YC +WRWC H+A
Sbjct: 477 WPYHRYCAGLWRWCVAHRA 533
>TC8037 similar to UP|AAR14273 (AAR14273) Predicted protein, partial (5%)
Length = 895
Score = 28.9 bits (63), Expect = 5.9
Identities = 19/62 (30%), Positives = 29/62 (46%)
Frame = +1
Query: 32 FMRWGMLIYCRRIWRWCEYHKALGHTTDNCWNLRREIDRLIKAGHLANFVKDTATPEVAK 91
F W CR RW ++H +C + E+D+ K+GH+ N + TAT +
Sbjct: 70 FRPWNYWDVCRH--RWADWHLV------SCGS-GIELDQTNKSGHITNL*RITATAAITI 222
Query: 92 IT 93
IT
Sbjct: 223 IT 228
>BP041751
Length = 471
Score = 28.5 bits (62), Expect = 7.7
Identities = 11/29 (37%), Positives = 15/29 (50%)
Frame = -1
Query: 33 MRWGMLIYCRRIWRWCEYHKALGHTTDNC 61
+R G + RR+WRWC + LG C
Sbjct: 285 LRGGPDLRFRRLWRWCGCRRRLGSLCRRC 199
>TC16027
Length = 653
Score = 28.5 bits (62), Expect = 7.7
Identities = 21/60 (35%), Positives = 30/60 (50%)
Frame = +2
Query: 1283 RLTYGTDAMLSVEIENQSWRVARFNENDNGENLIANLIMLPEEQREAHIRNEAGKVKVAR 1342
RL Y TDA L +E ++W V + ++ E L PEE R A + +E KV+R
Sbjct: 299 RLKYCTDACLRRYLEARNWNVDK-SKKMLEETLKWRSTYKPEEIRWAEVAHEGETGKVSR 475
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.135 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,600,346
Number of Sequences: 28460
Number of extensions: 271607
Number of successful extensions: 1277
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 1263
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1276
length of query: 1414
length of database: 4,897,600
effective HSP length: 102
effective length of query: 1312
effective length of database: 1,994,680
effective search space: 2617020160
effective search space used: 2617020160
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0114.12