BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0112.15
(994 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF177509 65 4e-11
BP030148 52 4e-07
AV415611 42 5e-04
TC17747 homologue to UP|Q8WML4 (Q8WML4) MUC1 protein precursor, ... 35 0.077
AV422430 33 0.17
BP083536 33 0.22
TC11009 similar to GB|AAP21377.1|30102918|BT006569 At1g47970 {Ar... 33 0.29
TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%) 32 0.50
TC8192 similar to UP|Q8L7U1 (Q8L7U1) AT4g39970/T5J17_140, partia... 32 0.65
AV411437 31 0.85
BI419972 30 1.9
TC9068 similar to UP|SCWA_YEAST (Q04951) Probable family 17 gluc... 29 3.2
BP085370 29 3.2
TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly pr... 29 4.2
TC10053 similar to UP|CHLI_SOYBN (P93162) Magnesium-chelatase su... 29 4.2
TC15397 similar to UP|CALX_SOYBN (Q39817) Calnexin homolog precu... 29 4.2
AV406521 28 5.5
TC19936 homologue to UP|Q9SAK5 (Q9SAK5) T8K14.15 protein, partia... 28 7.2
TC18010 similar to UP|MFPA_BRANA (O49809) Glyoxysomal fatty acid... 28 7.2
TC8259 similar to UP|HMGL_SOYBN (P26585) HMG1/2-like protein (SB... 28 7.2
>BF177509
Length = 472
Score = 65.5 bits (158), Expect = 4e-11
Identities = 37/137 (27%), Positives = 70/137 (51%)
Frame = +1
Query: 675 LPPKMWTRSAYSRDTQCDLQVNNMCEAFNRAILEYRDKPIITLLERLKFYLNNRIVKQKQ 734
+PP++W +AY + N+ E+ N ILE PII ++E ++ L ++++
Sbjct: 31 IPPRLWA-TAYFEGQRFGHLTANIVESLNTWILEASGLPIIQMMECIRRQLMTWFNERRE 207
Query: 735 LMLRWRTALCPMIQQKLEDSKRNSDKWFANWMGDEYMSLFEVSRDNEKYSVNLESRSCAC 794
++W + L P ++ + ++ + + + + FEV V++ +R C C
Sbjct: 208 TSMQWASILVPSAERCVAEALERARTYQVLRANE---AEFEVISHEGSNIVDIRNRCCLC 378
Query: 795 RRWDLSGIPCAHAVASM 811
R W L G+PCAHAVA++
Sbjct: 379 RGWQLYGLPCAHAVAAL 429
>BP030148
Length = 398
Score = 52.4 bits (124), Expect = 4e-07
Identities = 27/102 (26%), Positives = 54/102 (52%)
Frame = +3
Query: 618 HRLCIKHLYGNFRKKYPGAEMKQALWRAARASTLPEWYAAMEVMKELNEAAFQDMMNLPP 677
H C++HL +FRK++ + LW AA T+ E+ + + ++E+++ A + +PP
Sbjct: 90 HGFCMRHLSDSFRKEFNNTMLVNLLWEAAHVLTVIEFESKILEIEEISQDAAYWIRRIPP 269
Query: 678 KMWTRSAYSRDTQCDLQVNNMCEAFNRAILEYRDKPIITLLE 719
++W +AY + N+ E+ + IL+ PI+ + E
Sbjct: 270 RLWA-TAYFEGQRFGHLTANIVESLDTWILDASGLPIVQMTE 392
>AV415611
Length = 219
Score = 42.0 bits (97), Expect = 5e-04
Identities = 15/38 (39%), Positives = 24/38 (62%)
Frame = +3
Query: 772 SLFEVSRDNEKYSVNLESRSCACRRWDLSGIPCAHAVA 809
S +EV D V+++ C+C+ W L+G+PC HA+A
Sbjct: 72 STYEVCGDTPTEVVDIDRWECSCKTWQLTGVPCCHAIA 185
>TC17747 homologue to UP|Q8WML4 (Q8WML4) MUC1 protein precursor, partial
(4%)
Length = 507
Score = 34.7 bits (78), Expect = 0.077
Identities = 19/54 (35%), Positives = 27/54 (49%)
Frame = +2
Query: 122 APNSAPVNDAPNSPEVVHEVPNSAPVNDALNSSSALVHEVPNSAPVNDALNSSS 175
AP S+PV +P +P P S+P + SSS + HE D+ +SSS
Sbjct: 146 APTSSPVASSPAAPTPQSWSPGSSPAPSPMGSSSLISHEGVEGGRFTDSDSSSS 307
>AV422430
Length = 415
Score = 33.5 bits (75), Expect = 0.17
Identities = 16/39 (41%), Positives = 20/39 (51%)
Frame = +2
Query: 269 FEDEDGDSSDLDSPSDDEEEGRSKRLPRFKAVQDGEEVK 307
F DED DLD SD ++E LP FKA+ + K
Sbjct: 293 FGDEDSPDDDLDESSDSDDETELDELPPFKALTKAQVEK 409
>BP083536
Length = 377
Score = 33.1 bits (74), Expect = 0.22
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = +1
Query: 789 SRSCACRRWDLSGIPCAHAVASMWF 813
S++C C W+L IPC HA+A + +
Sbjct: 298 SKACCCNLWELVEIPCRHALAGVHY 372
>TC11009 similar to GB|AAP21377.1|30102918|BT006569 At1g47970 {Arabidopsis
thaliana;}, partial (44%)
Length = 700
Score = 32.7 bits (73), Expect = 0.29
Identities = 26/114 (22%), Positives = 43/114 (36%)
Frame = +2
Query: 180 EVPNSAPVNEPLNESEDESYIPGSDEYEGESEDVSVDDSDYEENWDWAEVLPAESLINIA 239
+ P ++ + E+E + G G+ +D DD D +E + E L E L+
Sbjct: 83 DAPGGGDDDDDEEDEEEEGGVEGGRGGGGDPDDDDDDDDDDDEEEEEEEDLGTEYLVRRT 262
Query: 240 TADRDPPFVQGSVDVGNGARIEEPATFSDFEDEDGDSSDLDSPSDDEEEGRSKR 293
A E+ SDFE E+ + D D+ ++ SKR
Sbjct: 263 VA-----------------AAEDEEASSDFEPEEDEGDDNDNDDGEKAGVPSKR 373
>TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%)
Length = 1008
Score = 32.0 bits (71), Expect = 0.50
Identities = 28/110 (25%), Positives = 47/110 (42%), Gaps = 9/110 (8%)
Frame = -2
Query: 189 EPLNESEDESYIPGSDEYEGESEDVSVDDSDYEENWDWA-----EVLPAESLINIATADR 243
E ++SED+ +++ E ED + D+ D +++ D E L +E
Sbjct: 659 ETKSQSEDK-------QHDAEGEDGNDDEGDEDDDGDGPFGEGEEDLSSEDGAGYGNNSN 501
Query: 244 DPPFVQGSVDVGNGARIEEPATFSDFEDEDG----DSSDLDSPSDDEEEG 289
+ + + + G GA D EDEDG D D D D+E++G
Sbjct: 500 NKSNSKKAPEGGPGAGAGPEENGEDEEDEDGEDQEDDDDDDEDDDEEDDG 351
>TC8192 similar to UP|Q8L7U1 (Q8L7U1) AT4g39970/T5J17_140, partial (80%)
Length = 1098
Score = 31.6 bits (70), Expect = 0.65
Identities = 22/84 (26%), Positives = 41/84 (48%), Gaps = 5/84 (5%)
Frame = +2
Query: 433 KVKAVEMIQGETLDQYANLRKYAAELLRSNPGSTVIIKSTKGAKGPVFERIYVCFAATKT 492
+ K ++ +QG ++Y ++ K ++ PG ++ K A +++ VC AATK+
Sbjct: 296 QAKLIDTLQGWKTERYKDIIKSGT--VKPRPGVLRLMDEAKDAG----KKLAVCSAATKS 457
Query: 493 AFATTCRPLIGLD-----GCFLKG 511
+ LIG++ CFL G
Sbjct: 458 SVILCLENLIGIERFQSLDCFLAG 529
>AV411437
Length = 417
Score = 31.2 bits (69), Expect = 0.85
Identities = 27/97 (27%), Positives = 39/97 (39%), Gaps = 6/97 (6%)
Frame = +2
Query: 245 PPFVQGSVDVGNGARIEEPATFSDFEDEDGDSSDLDSPSDDEEEGRSKRLPRFKAVQDGE 304
PP S EE + E+EDG +L+ DDE + L + GE
Sbjct: 8 PPLAAVSGSSAQETIAEEEEEEEEEEEEDGVDGELEQFDDDETDSEDDEL---ETEGGGE 178
Query: 305 EVKFQVGHTFSS------KDLFKQAVKDYALQQKKDL 335
QVG + SS K+ K V +++KK+L
Sbjct: 179 LEDAQVGGSTSSPLVEKRKERLKLEVPSLTVKEKKEL 289
>BI419972
Length = 498
Score = 30.0 bits (66), Expect = 1.9
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = +1
Query: 270 EDEDGDSSDLDSPSDDEEEG 289
+DEDGD D D+ DD++EG
Sbjct: 358 DDEDGDDEDDDADDDDDDEG 417
>TC9068 similar to UP|SCWA_YEAST (Q04951) Probable family 17 glucosidase
SCW10 precursor (Soluble cell wall protein 10) ,
partial (6%)
Length = 1209
Score = 29.3 bits (64), Expect = 3.2
Identities = 12/37 (32%), Positives = 19/37 (50%)
Frame = +2
Query: 259 RIEEPATFSDFEDEDGDSSDLDSPSDDEEEGRSKRLP 295
R+ P D +D+D D D D DD+++ K+ P
Sbjct: 62 RLFSPKPDDDDDDDDDDDDDDDDDDDDDDDDGDKKQP 172
>BP085370
Length = 381
Score = 29.3 bits (64), Expect = 3.2
Identities = 16/47 (34%), Positives = 26/47 (55%)
Frame = -2
Query: 133 NSPEVVHEVPNSAPVNDALNSSSALVHEVPNSAPVNDALNSSSALVH 179
NSP V P ++PV+ LNS S ++ + +ND+L + S + H
Sbjct: 359 NSPSTVIFSPENSPVSK-LNSKSDVLRSEHSLEIINDSLENDSCVTH 222
>TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly protein
1-like protein 3, partial (81%)
Length = 1298
Score = 28.9 bits (63), Expect = 4.2
Identities = 14/29 (48%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Frame = +2
Query: 262 EPATFSDFE--DEDGDSSDLDSPSDDEEE 288
E + F D E DEDGD + + DDEEE
Sbjct: 749 EQSDFEDIEEDDEDGDEDEDEDDDDDEEE 835
Score = 27.7 bits (60), Expect = 9.4
Identities = 14/39 (35%), Positives = 20/39 (50%)
Frame = +2
Query: 261 EEPATFSDFEDEDGDSSDLDSPSDDEEEGRSKRLPRFKA 299
+E D +DE+ + D D D+E EG+SK KA
Sbjct: 794 DEDEDEDDDDDEEEEDDDDDDEDDEEGEGKSKSKSGSKA 910
>TC10053 similar to UP|CHLI_SOYBN (P93162) Magnesium-chelatase subunit chlI,
chloroplast precursor (Mg-protoporphyrin IX chelatase)
, partial (58%)
Length = 802
Score = 28.9 bits (63), Expect = 4.2
Identities = 18/82 (21%), Positives = 42/82 (50%), Gaps = 4/82 (4%)
Frame = -3
Query: 354 PFYLRLSKTDSRSYWLL----VSLEADHTCCRSASNRQAKTKYLARKFMPIIRHTPNITV 409
PFY LS ++ +W+L + + +++ R N++ ++ L +P +T N+ +
Sbjct: 560 PFYYTLSHFNTHEFWILWV*VIWVSSNNLDFRK*INQRPNSRGLPCSPVPHDHYTSNLGI 381
Query: 410 NALIEEARLRWGILLGKWKAYR 431
N + E+A+ ++L + + R
Sbjct: 380 NHIKEKAKFH--LILSNYSSKR 321
>TC15397 similar to UP|CALX_SOYBN (Q39817) Calnexin homolog precursor,
partial (58%)
Length = 1103
Score = 28.9 bits (63), Expect = 4.2
Identities = 23/97 (23%), Positives = 38/97 (38%), Gaps = 3/97 (3%)
Frame = +2
Query: 193 ESEDESYIPGSDEYEGE---SEDVSVDDSDYEENWDWAEVLPAESLINIATADRDPPFVQ 249
E + ++ I S+++E S+ + D E+WD +P + D D P
Sbjct: 722 EEKKKANILSSEDFEPSLIPSKTIPDPDDKKPEDWDERAKIPDPDAVKPEDWDEDAPMEI 901
Query: 250 GSVDVGNGARIEEPATFSDFEDEDGDSSDLDSPSDDE 286
+ E+P + D E E+ D D P D E
Sbjct: 902 EDEEA------EKPEGWLDDEPEEVDDPDATKPEDWE 994
>AV406521
Length = 267
Score = 28.5 bits (62), Expect = 5.5
Identities = 10/32 (31%), Positives = 19/32 (59%)
Frame = +3
Query: 618 HRLCIKHLYGNFRKKYPGAEMKQALWRAARAS 649
H C++HL + K++ + + LW+AA A+
Sbjct: 171 HTFCMRHLSESIGKEFKNSRLVHLLWKAAYAT 266
>TC19936 homologue to UP|Q9SAK5 (Q9SAK5) T8K14.15 protein, partial (23%)
Length = 444
Score = 28.1 bits (61), Expect = 7.2
Identities = 15/51 (29%), Positives = 28/51 (54%)
Frame = -2
Query: 436 AVEMIQGETLDQYANLRKYAAELLRSNPGSTVIIKSTKGAKGPVFERIYVC 486
A+++++GETL + + + L+R++ S I K+ GP R +VC
Sbjct: 377 ALKVVKGETLHTHHPHDGFRSSLIRASELSDSINKAFMELDGPAEARFWVC 225
>TC18010 similar to UP|MFPA_BRANA (O49809) Glyoxysomal fatty acid
beta-oxidation multifunctional protein MFP-a [Includes:
Enoyl-CoA hydratase ; 3-2-trans-enoyl-CoA isomerase ;
3-hydroxybutyryl-CoA epimerase ; 3-hydroxyacyl-CoA
dehydrogenase , partial (7%)
Length = 454
Score = 28.1 bits (61), Expect = 7.2
Identities = 17/38 (44%), Positives = 20/38 (51%), Gaps = 1/38 (2%)
Frame = -1
Query: 789 SRSCACRRWDLSGIPCAHAVASMWFG-RRVPEDYVHSS 825
+R CAC R LSGIP A A G + P + HSS
Sbjct: 148 NRDCACSREALSGIPLAALAAK*GHGLKNSPYCFDHSS 35
>TC8259 similar to UP|HMGL_SOYBN (P26585) HMG1/2-like protein (SB11
protein), partial (92%)
Length = 942
Score = 28.1 bits (61), Expect = 7.2
Identities = 18/70 (25%), Positives = 29/70 (40%), Gaps = 9/70 (12%)
Frame = +2
Query: 226 WAEVLPAESLINIATADRDPPFVQGSVDVGNGARIEEPA---------TFSDFEDEDGDS 276
W + AE +A A++ + ++ N + E PA + S+ D+D D
Sbjct: 422 WKSMSEAEKAPYVAKAEKRKADYEKTMKAYNKKQAEGPAAADEEESEKSVSEVNDQDEDD 601
Query: 277 SDLDSPSDDE 286
D D DDE
Sbjct: 602 DDSDEDDDDE 631
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.326 0.139 0.435
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,940,036
Number of Sequences: 28460
Number of extensions: 234660
Number of successful extensions: 1685
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 1583
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1657
length of query: 994
length of database: 4,897,600
effective HSP length: 99
effective length of query: 895
effective length of database: 2,080,060
effective search space: 1861653700
effective search space used: 1861653700
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0112.15