BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0112.1
(547 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14463 similar to UP|Q9SLF1 (Q9SLF1) Nodulin-like protein (At2g... 671 0.0
TC14658 similar to UP|Q9SLF1 (Q9SLF1) Nodulin-like protein (At2g... 394 e-110
TC12806 UP|O81120 (O81120) Nodule-specific protein Nlj70, complete 300 4e-82
TC18479 similar to UP|O80960 (O80960) Nodulin-like protein (At2g... 136 8e-33
AV426407 120 7e-28
AV417881 94 6e-20
TC18780 weakly similar to UP|Q9LMD4 (Q9LMD4) F14D16.8, partial (... 82 2e-16
AU252186 66 1e-11
AV778887 54 5e-08
BP074800 42 3e-04
TC14163 30 1.0
BP052416 29 1.7
BP078898 28 2.9
BI420256 28 2.9
TC14388 similar to UP|CHMT_MEDSA (P93324) Isoliquiritigenin 2'-O... 28 3.8
TC10398 weakly similar to UP|Q9FII5 (Q9FII5) Receptor protein ki... 27 6.5
TC8075 similar to UP|E13B_PEA (Q03467) Glucan endo-1,3-beta-gluc... 27 8.5
>TC14463 similar to UP|Q9SLF1 (Q9SLF1) Nodulin-like protein
(At2g16660/T24I21.7), partial (40%)
Length = 1122
Score = 671 bits (1731), Expect = 0.0
Identities = 338/338 (100%), Positives = 338/338 (100%)
Frame = +1
Query: 1 MGATEFSVSTAGKWLGLVSAIWIQSISGNNYTFSNYSHALKSLMHLTQLQLNNLSVAKDV 60
MGATEFSVSTAGKWLGLVSAIWIQSISGNNYTFSNYSHALKSLMHLTQLQLNNLSVAKDV
Sbjct: 109 MGATEFSVSTAGKWLGLVSAIWIQSISGNNYTFSNYSHALKSLMHLTQLQLNNLSVAKDV 288
Query: 61 GKAFGLLAGLSSDFLPTWAILLIGSVEGLIGYGVQWLVVGQFINPLPYWQMCVFLCLGGN 120
GKAFGLLAGLSSDFLPTWAILLIGSVEGLIGYGVQWLVVGQFINPLPYWQMCVFLCLGGN
Sbjct: 289 GKAFGLLAGLSSDFLPTWAILLIGSVEGLIGYGVQWLVVGQFINPLPYWQMCVFLCLGGN 468
Query: 121 STTWMNTAVLVTSIRNFRRNRGPVSGILKGFVGLSTAIFTNLCSALFADDPASFLLMLAL 180
STTWMNTAVLVTSIRNFRRNRGPVSGILKGFVGLSTAIFTNLCSALFADDPASFLLMLAL
Sbjct: 469 STTWMNTAVLVTSIRNFRRNRGPVSGILKGFVGLSTAIFTNLCSALFADDPASFLLMLAL 648
Query: 181 IPFAVCIAGVFFLREVPPAKTVTAEDDSEEASYFGVCNAVAVVVAVYLLAYGFVPSPSSL 240
IPFAVCIAGVFFLREVPPAKTVTAEDDSEEASYFGVCNAVAVVVAVYLLAYGFVPSPSSL
Sbjct: 649 IPFAVCIAGVFFLREVPPAKTVTAEDDSEEASYFGVCNAVAVVVAVYLLAYGFVPSPSSL 828
Query: 241 VSRVFVAVLLVLLASPVGIPVYSYFKGRKSKSNRDVEGQRVTEPLLIQSDDKENGAVTVA 300
VSRVFVAVLLVLLASPVGIPVYSYFKGRKSKSNRDVEGQRVTEPLLIQSDDKENGAVTVA
Sbjct: 829 VSRVFVAVLLVLLASPVGIPVYSYFKGRKSKSNRDVEGQRVTEPLLIQSDDKENGAVTVA 1008
Query: 301 AEEEVVVVKGRPAVGEEHTIWEAMRSVDFWILFTSFLC 338
AEEEVVVVKGRPAVGEEHTIWEAMRSVDFWILFTSFLC
Sbjct: 1009AEEEVVVVKGRPAVGEEHTIWEAMRSVDFWILFTSFLC 1122
>TC14658 similar to UP|Q9SLF1 (Q9SLF1) Nodulin-like protein
(At2g16660/T24I21.7), partial (36%)
Length = 976
Score = 394 bits (1013), Expect = e-110
Identities = 201/201 (100%), Positives = 201/201 (100%)
Frame = +3
Query: 347 MNNMGQIGLALGYTDVSLFVSLTSIWGFFGRIISGSVSELFIKKAATPRPLWNAASQILM 406
MNNMGQIGLALGYTDVSLFVSLTSIWGFFGRIISGSVSELFIKKAATPRPLWNAASQILM
Sbjct: 3 MNNMGQIGLALGYTDVSLFVSLTSIWGFFGRIISGSVSELFIKKAATPRPLWNAASQILM 182
Query: 407 AVGYILLAMALPGSLYIGSIVVGICYGVRLAITVPTASELFGLKYYGLIYNILILNLPLG 466
AVGYILLAMALPGSLYIGSIVVGICYGVRLAITVPTASELFGLKYYGLIYNILILNLPLG
Sbjct: 183 AVGYILLAMALPGSLYIGSIVVGICYGVRLAITVPTASELFGLKYYGLIYNILILNLPLG 362
Query: 467 SFLFSGLLAGILYDREATATEGGGNTCVGGHCYRLVFIVMAAACIVGFFLDILLSIRTKN 526
SFLFSGLLAGILYDREATATEGGGNTCVGGHCYRLVFIVMAAACIVGFFLDILLSIRTKN
Sbjct: 363 SFLFSGLLAGILYDREATATEGGGNTCVGGHCYRLVFIVMAAACIVGFFLDILLSIRTKN 542
Query: 527 VYNKISMSKKSKKSLGTSSSQ 547
VYNKISMSKKSKKSLGTSSSQ
Sbjct: 543 VYNKISMSKKSKKSLGTSSSQ 605
>TC12806 UP|O81120 (O81120) Nodule-specific protein Nlj70, complete
Length = 2069
Score = 300 bits (768), Expect = 4e-82
Identities = 186/555 (33%), Positives = 293/555 (52%), Gaps = 19/555 (3%)
Frame = +1
Query: 12 GKWLGLVSAIWIQSISGNNYTFSNYSHALKSLMHLTQLQLNNLSVAKDVGKAFGLLAGLS 71
G+W ++++ I ++SG +Y FS YS +K + Q LN LS KDVG G+L+GL
Sbjct: 118 GRWFMMLASFMIMAVSGASYMFSLYSREIKFALGYDQSTLNLLSFFKDVGSNIGILSGLI 297
Query: 72 SDFLPTWAILLIGSVEGLIGYGVQWLVVGQFINPLPYWQMCVFLCLGGNSTTWMNTAVLV 131
++ P W +L +G+V G+ + WL V + I W +C+++ +G NS + NTAV+V
Sbjct: 298 NEVTPPWVVLSMGAVLNFFGFFMIWLAVAKKIANPRVWHLCLYIVIGSNSHCFTNTAVMV 477
Query: 132 TSIRNFRRNRGPVSGILKGFVGLSTAIFTNLCSALFADDPASFLLMLALIPFAVCIAGVF 191
TS++NF RG V GIL G++ LS I T L A F +D S +L++A +P A +
Sbjct: 478 TSVKNFPGIRGIVLGILGGYLSLSAIIITQLYYAFFINDSQSMILIMACLPTATAL---I 648
Query: 192 FLREVPPAKTVTAEDDSE---EASYFGVCNAVAVVVAVYL-LAYGFVPSPSSLVSRVFVA 247
L + K++ ++DS+ Y + A +++ + L +++ F S +
Sbjct: 649 LLPVIKNHKSIQQKNDSKVFYRFIYLVLALAGFLMIMIILQISFNFTQSEYYATT----T 816
Query: 248 VLLVLLASPVGIPVYSYFKGRKSKSNRDVEGQRVTEPLLIQSDDKENGAVTVAAEEEVVV 307
V+L+LL P+ + + K KSK + + P + + K N + E +
Sbjct: 817 VMLLLLTLPLAVVIVEDCKIWKSKQEL-INCENPPRP--VDTTTKSNELKSEQTIPEGLS 987
Query: 308 ----VKGRPAVGEEHTIWEAMRSVDFWILFTSFLCGVGTGLAVMNNMGQIGLALGYTD-- 361
+ P GE+HT+ +A+ S+D ILF + +CG G+ L V NN+ QIG +LGY
Sbjct: 988 CWQNILRHPERGEDHTVLQAIFSLDMVILFFATVCGFGSNLTVYNNLSQIGKSLGYPSYT 1167
Query: 362 VSLFVSLTSIWGFFGRIISGSVSELFIKKAATPRPLWNAASQILMAVGYILLAMALPGSL 421
++ FVSL SIW F G+I G +SE I K PRPL +L +G++L+A +P L
Sbjct: 1168ITTFVSLMSIWIFLGKIAQGVLSEFMITKLKLPRPLMFTIVHVLSCIGHLLIAFNVPNGL 1347
Query: 422 YIGSIVVGICYGVRLAITVPTASELFGLKYYGLIYNILILNLPLGSFLFSGLLAGILYDR 481
Y SI +G C G I SELFGLK+Y +YN+ + P+GS+L + +AG LYDR
Sbjct: 1348YAASIFIGFCLGASWPIINSLISELFGLKHYSTLYNVGTVASPIGSYLLNVKVAGYLYDR 1527
Query: 482 EA---------TATEGGGNTCVGGHCYRLVFIVMAAACIVGFFLDILLSIRTKNVYNKIS 532
EA G C G CY+L +I++ A C+ G + +L +RT+ Y K
Sbjct: 1528EARRQMAALGLQRKPGEELNCNGSDCYKLAYIIITAVCLFGALVSFILVLRTRQFY-KTD 1704
Query: 533 MSKKSKKSLGTSSSQ 547
+ KK + T+ ++
Sbjct: 1705IYKKFTEEPRTAETK 1749
>TC18479 similar to UP|O80960 (O80960) Nodulin-like protein
(At2g39210/T16B24.15), partial (27%)
Length = 524
Score = 136 bits (343), Expect = 8e-33
Identities = 68/159 (42%), Positives = 99/159 (61%), Gaps = 2/159 (1%)
Frame = +2
Query: 312 PAVGEEHTIWEAMRSVDFWILFTSFLCGVGTGLAVMNNMGQIGLALGY--TDVSLFVSLT 369
P GE++TI +A+ S+D +LF + CGVG L ++N+ QIG +LGY + +S FVSL
Sbjct: 47 PERGEDYTILQALFSIDMILLFIAGTCGVGGTLTAIDNLSQIGTSLGYQKSSISTFVSLV 226
Query: 370 SIWGFFGRIISGSVSELFIKKAATPRPLWNAASQILMAVGYILLAMALPGSLYIGSIVVG 429
SIW + GR+ SG VSE + K PRPL + +L VG+IL+A +P LY S+++G
Sbjct: 227 SIWNYAGRVFSGFVSEHVLTKYNFPRPLMLTLTLLLSCVGHILIAFNVPNGLYAASVIIG 406
Query: 430 ICYGVRLAITVPTASELFGLKYYGLIYNILILNLPLGSF 468
C+G + + SELFGLKYY +YN + P+G +
Sbjct: 407 FCFGAQWPLLFAIISELFGLKYYSTLYNFGGVTSPIGLY 523
>AV426407
Length = 415
Score = 120 bits (300), Expect = 7e-28
Identities = 54/123 (43%), Positives = 87/123 (69%)
Frame = +2
Query: 14 WLGLVSAIWIQSISGNNYTFSNYSHALKSLMHLTQLQLNNLSVAKDVGKAFGLLAGLSSD 73
W+GL +A+W+Q SGN +TF+ YSH+LKS++ Q QL+ L VA D+G+ FG+L GL+ +
Sbjct: 50 WVGLGAAVWVQIASGNTFTFALYSHSLKSVLGFNQRQLSLLGVANDLGENFGILPGLACN 229
Query: 74 FLPTWAILLIGSVEGLIGYGVQWLVVGQFINPLPYWQMCVFLCLGGNSTTWMNTAVLVTS 133
P W +L +GS+ +GYGV +L + + ++ +PY+ + + L + N+T W+ TAVLVT+
Sbjct: 230 KFPPWLLLCVGSLTSFLGYGVLYLALTKTVS-IPYFVLWLALVIAANNTAWLTTAVLVTN 406
Query: 134 IRN 136
+RN
Sbjct: 407 MRN 415
>AV417881
Length = 431
Score = 94.0 bits (232), Expect = 6e-20
Identities = 52/140 (37%), Positives = 73/140 (52%), Gaps = 16/140 (11%)
Frame = +2
Query: 14 WLGLVSAIWIQSISGNNYTFSNYSHALKSLMHLTQLQLNNLSVAKDVGKAFGLLAGL--- 70
W G+ +AIWIQ G +YTFS YS LKS Q L+ +SV KD+G FG+L+GL
Sbjct: 2 WTGVAAAIWIQWSCGASYTFSIYSPILKSTQGYDQSTLDTVSVFKDIGANFGVLSGLLYS 181
Query: 71 -------------SSDFLPTWAILLIGSVEGLIGYGVQWLVVGQFINPLPYWQMCVFLCL 117
+S W ++ +G+V+ +G+ W V I+P P MC F L
Sbjct: 182 AVTPYFNRRDTKWTSSLAGPWIVVAVGAVQCFVGFFFIWAAVVGLIDPPPVPVMCFFAWL 361
Query: 118 GGNSTTWMNTAVLVTSIRNF 137
N T++NT +VT +RNF
Sbjct: 362 ASNGQTFLNTTNVVTGLRNF 421
>TC18780 weakly similar to UP|Q9LMD4 (Q9LMD4) F14D16.8, partial (18%)
Length = 393
Score = 82.0 bits (201), Expect = 2e-16
Identities = 37/113 (32%), Positives = 64/113 (55%)
Frame = +3
Query: 423 IGSIVVGICYGVRLAITVPTASELFGLKYYGLIYNILILNLPLGSFLFSGLLAGILYDRE 482
+G ++VGI YG ++ SE+FG+K+ G I+N + P+GS++ S + G +YDRE
Sbjct: 3 LGPVLVGISYGAHWSLMPTITSEIFGVKHMGTIFNTIAAASPIGSYILSVRVVGYIYDRE 182
Query: 483 ATATEGGGNTCVGGHCYRLVFIVMAAACIVGFFLDILLSIRTKNVYNKISMSK 535
A + N+C G HC+ L F ++A + F + + L RT+ Y + + +
Sbjct: 183 AIEED---NSCFGIHCFMLSFFILAGVAFLSFLVGLALYFRTQKFYKLVVLRR 332
>AU252186
Length = 350
Score = 66.2 bits (160), Expect = 1e-11
Identities = 33/75 (44%), Positives = 49/75 (65%)
Frame = -2
Query: 40 LKSLMHLTQLQLNNLSVAKDVGKAFGLLAGLSSDFLPTWAILLIGSVEGLIGYGVQWLVV 99
LKS + ++Q+QLN L+ A D+GK FG +GL+ +LP ++ I + G IGYG+QWLV+
Sbjct: 349 LKSALDISQVQLNYLATANDMGKVFGWSSGLALMYLPLSFVMFIAASMGFIGYGLQWLVI 170
Query: 100 GQFINPLPYWQMCVF 114
I LPY+ + F
Sbjct: 169 NHVIT-LPYFLVISF 128
>AV778887
Length = 483
Score = 54.3 bits (129), Expect = 5e-08
Identities = 29/78 (37%), Positives = 42/78 (53%), Gaps = 5/78 (6%)
Frame = -3
Query: 456 YNILILNLPLGSFLFSGLLAGILYDRE-----ATATEGGGNTCVGGHCYRLVFIVMAAAC 510
+N + L PLG+ FS LLAG +YD E A G +C G C++L F V+A
Sbjct: 478 FNFMCLANPLGALPFSFLLAGRVYDNEVARPLALGLLDSGVSCFGADCFKLTFFVLAGVS 299
Query: 511 IVGFFLDILLSIRTKNVY 528
G F ++L++R + VY
Sbjct: 298 AAGAFSSLILTMRIRPVY 245
>BP074800
Length = 381
Score = 42.0 bits (97), Expect = 3e-04
Identities = 15/35 (42%), Positives = 24/35 (67%)
Frame = -3
Query: 493 CVGGHCYRLVFIVMAAACIVGFFLDILLSIRTKNV 527
C GG CY+L FI++ C++G + +L++RTK V
Sbjct: 331 CNGGECYKLSFIIITVVCLLGALVSFILALRTKKV 227
>TC14163
Length = 1712
Score = 30.0 bits (66), Expect = 1.0
Identities = 17/57 (29%), Positives = 30/57 (51%)
Frame = +1
Query: 15 LGLVSAIWIQSISGNNYTFSNYSHALKSLMHLTQLQLNNLSVAKDVGKAFGLLAGLS 71
+G ++ + + S++GN +T ++ L HLTQLQL N S+ + L L+
Sbjct: 547 IGNLTRLDVLSLTGNRFT-GTIPSSVGGLTHLTQLQLGNNSLTGTIPATIARLKNLT 714
>BP052416
Length = 392
Score = 29.3 bits (64), Expect = 1.7
Identities = 20/57 (35%), Positives = 31/57 (54%)
Frame = -3
Query: 147 ILKGFVGLSTAIFTNLCSALFADDPASFLLMLALIPFAVCIAGVFFLREVPPAKTVT 203
++KG+ GL+ +LC L A + L +++IPF VC+ + L E P TVT
Sbjct: 189 MMKGYPGLARHKKNHLC--LVAQNRT*KSL*ISIIPFHVCLLYLSCLPEPPVKLTVT 25
>BP078898
Length = 378
Score = 28.5 bits (62), Expect = 2.9
Identities = 23/67 (34%), Positives = 33/67 (48%)
Frame = +2
Query: 15 LGLVSAIWIQSISGNNYTFSNYSHALKSLMHLTQLQLNNLSVAKDVGKAFGLLAGLSSDF 74
LG VS I SIS + +++ SH L+ + +T A D FGL+ ++ F
Sbjct: 149 LG*VSNIPFPSISSS**SYNIRSHLLQLNIIIT---FTPKWSATDTLSFFGLITTMAPIF 319
Query: 75 LPTWAIL 81
LP W IL
Sbjct: 320 LPPWTIL 340
>BI420256
Length = 524
Score = 28.5 bits (62), Expect = 2.9
Identities = 11/43 (25%), Positives = 24/43 (55%)
Frame = -1
Query: 499 YRLVFIVMAAACIVGFFLDILLSIRTKNVYNKISMSKKSKKSL 541
++L+F VM C++G+FL +L + + N ++ K ++
Sbjct: 458 WKLIFSVMEILCLLGYFLQHVLLLLLQTFQNLLTFPKHGSNAV 330
>TC14388 similar to UP|CHMT_MEDSA (P93324) Isoliquiritigenin
2'-O-methyltransferase (Chalcone O-methyltransferase)
(ChOMT) , partial (45%)
Length = 620
Score = 28.1 bits (61), Expect = 3.8
Identities = 15/44 (34%), Positives = 21/44 (47%)
Frame = +3
Query: 329 FWILFTSFLCGVGTGLAVMNNMGQIGLALGYTDVSLFVSLTSIW 372
F L TS L + G + NN+ AL + LFV+L+ W
Sbjct: 354 FQFLITSCLLHLVVGKGLRNNLSVWASALDFPGFKLFVALSLFW 485
>TC10398 weakly similar to UP|Q9FII5 (Q9FII5) Receptor protein kinase-like
protein, partial (5%)
Length = 602
Score = 27.3 bits (59), Expect = 6.5
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = -2
Query: 227 YLLAYGFVPSPSSLVSRVFVAVLLVLLASP 256
+LL YG P P L+ + A +LVL P
Sbjct: 187 HLLPYGSTPGPGILIQHIINAAILVLDLGP 98
>TC8075 similar to UP|E13B_PEA (Q03467) Glucan endo-1,3-beta-glucosidase
precursor ((1->3)-beta-glucan endohydrolase)
((1->3)-beta-glucanase) (Beta-1,3-endoglucanase) ,
partial (88%)
Length = 1437
Score = 26.9 bits (58), Expect = 8.5
Identities = 12/37 (32%), Positives = 23/37 (61%)
Frame = +2
Query: 413 LAMALPGSLYIGSIVVGICYGVRLAITVPTASELFGL 449
L + L +L+I +G+CYG+ + +P+A+E+ L
Sbjct: 152 LLLLLTTNLHIADAQIGVCYGM-MGNNLPSANEVVAL 259
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.324 0.139 0.417
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,816,557
Number of Sequences: 28460
Number of extensions: 144395
Number of successful extensions: 660
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 649
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 652
length of query: 547
length of database: 4,897,600
effective HSP length: 95
effective length of query: 452
effective length of database: 2,193,900
effective search space: 991642800
effective search space used: 991642800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0112.1