BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0103.5
(275 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8970 59 4e-17
TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 54 4e-08
TC18927 similar to PIR|AI2934|AI2934 chromate transport protein ... 48 2e-06
AV408058 33 1e-05
BP052706 43 5e-05
TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, part... 41 2e-04
BI418821 39 7e-04
TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, par... 38 0.002
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 36 0.008
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 35 0.014
TC13053 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 33 0.040
TC11804 similar to GB|AAO63413.1|28950979|BT005349 At5g01420 {Ar... 33 0.053
BP033819 32 0.090
AV421607 31 0.26
AV773507 30 0.45
BP039068 30 0.58
TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 30 0.58
BP079571 30 0.58
BP033671 29 0.76
TC12773 29 0.99
>TC8970
Length = 609
Score = 58.9 bits (141), Expect(2) = 4e-17
Identities = 31/46 (67%), Positives = 35/46 (75%), Gaps = 1/46 (2%)
Frame = +1
Query: 35 AFDEDPDFMADRGRVDNSSVGYGWLESFELVVTVIALI-RLTFGTG 79
AFD DPDFM D+ RV++S VGYGWLE LVV VIALI +L FG G
Sbjct: 145 AFDVDPDFMEDQMRVEDSGVGYGWLELLILVVMVIALIHQLYFGAG 282
Score = 44.7 bits (104), Expect(2) = 4e-17
Identities = 21/34 (61%), Positives = 25/34 (72%)
Frame = +2
Query: 1 MFVFGRSACCQASVSRFVMIRCAGKTRSEMSITE 34
MFVFGRSACCQ F +R +G+TRSEM+ TE
Sbjct: 44 MFVFGRSACCQTFNR*FKKVRRSGRTRSEMTTTE 145
>TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (26%)
Length = 555
Score = 53.5 bits (127), Expect = 4e-08
Identities = 25/63 (39%), Positives = 34/63 (53%)
Frame = +2
Query: 183 QRGMNRTGAGGPMRKFGHFANKCSVTGWRCFKCNREGHLAVNCKTKESLCFNCNQPGHFS 242
+RG +R ++ GHFA +C C C GH+A C TK SLC+NC +PGH +
Sbjct: 317 RRGFSRDNLCKNCKRPGHFARECPNVAI-CHNCGLPGHIASECTTK-SLCWNCKEPGHMA 490
Query: 243 QDC 245
C
Sbjct: 491 SSC 499
>TC18927 similar to PIR|AI2934|AI2934 chromate transport protein chrA
[imported] - Agrobacterium tumefaciens
(strain C58, Dupont) {Agrobacterium tumefaciens;},
partial (6%)
Length = 561
Score = 48.1 bits (113), Expect = 2e-06
Identities = 25/85 (29%), Positives = 44/85 (51%), Gaps = 3/85 (3%)
Frame = -2
Query: 183 QRGMNRTGAGGPMRKFGHFANKCSVTGWR---CFKCNREGHLAVNCKTKESLCFNCNQPG 239
QR NR + G FG+F + + + C +C+++GH A C + +C+NC + G
Sbjct: 425 QRPQNRGTSSGYSHSFGNFVPRPTQSDTSEIVCHRCSKKGHFANRCP--DLVCWNCQKTG 252
Query: 240 HFSQDCMALRGESSGNVGKGKQLAP 264
H +DC + E++ N ++ AP
Sbjct: 251 HSGKDCTNPKVEAATNAIAARRPAP 177
>AV408058
Length = 386
Score = 32.7 bits (73), Expect(2) = 1e-05
Identities = 15/30 (50%), Positives = 21/30 (70%)
Frame = +1
Query: 5 GRSACCQASVSRFVMIRCAGKTRSEMSITE 34
GR +S+ FV++R +GKTRSEM+ TE
Sbjct: 229 GRKLAKMSSMDSFVILRHSGKTRSEMTTTE 318
Score = 31.6 bits (70), Expect(2) = 1e-05
Identities = 14/23 (60%), Positives = 17/23 (73%)
Frame = +3
Query: 35 AFDEDPDFMADRGRVDNSSVGYG 57
AFD DPDFM D+ VD+ S+G G
Sbjct: 318 AFDVDPDFMHDQRGVDDGSMGDG 386
>BP052706
Length = 503
Score = 43.1 bits (100), Expect = 5e-05
Identities = 37/100 (37%), Positives = 50/100 (50%), Gaps = 2/100 (2%)
Frame = -2
Query: 1 MFVFGRSACCQASVSRFVMIRCAGKTRSEMSITEAFDEDPDFMA--DRGRVDNSSVGYGW 58
+FVFGRS C+ + FVM+R +G+ RSEM E DED FM+ D GR D + +
Sbjct: 358 LFVFGRSXNCKMVNNWFVMVRHSGQVRSEM--IEHSDEDSYFMSEKDAGR*DWAGICVAR 185
Query: 59 LESFELVVTVIALIRLTFGTGDTWKSGCYIVVSELSRVFW 98
+E + I FGT D + C +V S FW
Sbjct: 184 AYCYEALDVFTCNI---FGTTDAF---CKLV----SLAFW 95
>TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (56%)
Length = 598
Score = 41.2 bits (95), Expect = 2e-04
Identities = 19/61 (31%), Positives = 29/61 (47%), Gaps = 2/61 (3%)
Frame = +1
Query: 187 NRTGAGGPMRKFGHFANKCSVTGWRCFKCNREGHLAVNCKTKE--SLCFNCNQPGHFSQD 244
+R G GG R + RCF C +GH A +CK + + C+ C + GH ++
Sbjct: 316 SREGGGGRDRDREYMGRGPPPGSGRCFNCGIDGHWARDCKAGDWKNKCYRCGERGHIEKN 495
Query: 245 C 245
C
Sbjct: 496 C 498
Score = 35.4 bits (80), Expect = 0.011
Identities = 12/30 (40%), Positives = 17/30 (56%), Gaps = 2/30 (6%)
Frame = +1
Query: 199 GHFANKCSVTGWR--CFKCNREGHLAVNCK 226
GH+A C W+ C++C GH+ NCK
Sbjct: 412 GHWARDCKAGDWKNKCYRCGERGHIEKNCK 501
Score = 28.1 bits (61), Expect = 1.7
Identities = 9/16 (56%), Positives = 12/16 (74%)
Frame = +1
Query: 232 CFNCNQPGHFSQDCMA 247
CFNC GH+++DC A
Sbjct: 391 CFNCGIDGHWARDCKA 438
>BI418821
Length = 614
Score = 39.3 bits (90), Expect = 7e-04
Identities = 17/54 (31%), Positives = 23/54 (42%), Gaps = 8/54 (14%)
Frame = +2
Query: 209 GWRCFKCNREGHLAVNCKTKES--------LCFNCNQPGHFSQDCMALRGESSG 254
G C+ C GHLA +C + C+NC GH ++DC S G
Sbjct: 383 GGGCYNCGDTGHLARDCHRSNNNGGGGGGAACYNCGDAGHLARDCNRSNNNSGG 544
Score = 38.9 bits (89), Expect = 0.001
Identities = 19/62 (30%), Positives = 26/62 (41%), Gaps = 15/62 (24%)
Frame = +2
Query: 199 GHFANKCSVT--------GWRCFKCNREGHLAVNCKTKESL-------CFNCNQPGHFSQ 243
GH A C + G C+ C GHLA +C + C+NC GH ++
Sbjct: 413 GHLARDCHRSNNNGGGGGGAACYNCGDAGHLARDCNRSNNNSGGGGAGCYNCGDTGHLAR 592
Query: 244 DC 245
DC
Sbjct: 593 DC 598
Score = 28.5 bits (62), Expect = 1.3
Identities = 11/28 (39%), Positives = 16/28 (56%)
Frame = +2
Query: 232 CFNCNQPGHFSQDCMALRGESSGNVGKG 259
C+NC GH ++DC R ++G G G
Sbjct: 392 CYNCGDTGHLARDCH--RSNNNGGGGGG 469
>TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (62%)
Length = 684
Score = 38.1 bits (87), Expect = 0.002
Identities = 17/56 (30%), Positives = 25/56 (44%), Gaps = 2/56 (3%)
Frame = +2
Query: 192 GGPMRKFGHFANKCSVTGWRCFKCNREGHLAVNCKTKE--SLCFNCNQPGHFSQDC 245
GGP + RCF C +GH A +CK + + C+ C GH ++C
Sbjct: 311 GGPRGNREYLGRGPPPGSGRCFNCGLDGHWARDCKAGDWKNKCYRCGDRGHVERNC 478
Score = 33.9 bits (76), Expect = 0.031
Identities = 12/30 (40%), Positives = 17/30 (56%), Gaps = 2/30 (6%)
Frame = +2
Query: 199 GHFANKCSVTGWR--CFKCNREGHLAVNCK 226
GH+A C W+ C++C GH+ NCK
Sbjct: 392 GHWARDCKAGDWKNKCYRCGDRGHVERNCK 481
Score = 28.5 bits (62), Expect = 1.3
Identities = 9/16 (56%), Positives = 12/16 (74%)
Frame = +2
Query: 232 CFNCNQPGHFSQDCMA 247
CFNC GH+++DC A
Sbjct: 371 CFNCGLDGHWARDCKA 418
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 35.8 bits (81), Expect = 0.008
Identities = 17/48 (35%), Positives = 26/48 (53%)
Frame = +2
Query: 181 YQQRGMNRTGAGGPMRKFGHFANKCSVTGWRCFKCNREGHLAVNCKTK 228
Y++RG +R G R G+ ++ S G CFKC + GH A C ++
Sbjct: 329 YRERGRDRDDRGDRDRSRGYGGSRGS-NGGECFKCGKPGHFARECPSE 469
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 35.0 bits (79), Expect = 0.014
Identities = 14/33 (42%), Positives = 24/33 (72%)
Frame = +1
Query: 232 CFNCNQPGHFSQDCMALRGESSGNVGKGKQLAP 264
C+ C +PGHF+++C + RG S G +G G++ +P
Sbjct: 166 CYECGEPGHFARECRS-RGGSRG-LGSGRRRSP 258
>TC13053 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (9%)
Length = 450
Score = 33.5 bits (75), Expect = 0.040
Identities = 11/34 (32%), Positives = 18/34 (52%)
Frame = +3
Query: 212 CFKCNREGHLAVNCKTKESLCFNCNQPGHFSQDC 245
C C + GH++ +C +C NC GH + +C
Sbjct: 9 CRNCQQLGHMSRDCMGPLMICHNCGGRGHLAYEC 110
Score = 28.9 bits (63), Expect = 0.99
Identities = 10/16 (62%), Positives = 12/16 (74%)
Frame = +3
Query: 231 LCFNCNQPGHFSQDCM 246
+C NC Q GH S+DCM
Sbjct: 6 VCRNCQQLGHMSRDCM 53
Score = 26.6 bits (57), Expect = 4.9
Identities = 10/30 (33%), Positives = 13/30 (43%)
Frame = +3
Query: 196 RKFGHFANKCSVTGWRCFKCNREGHLAVNC 225
++ GH + C C C GHLA C
Sbjct: 21 QQLGHMSRDCMGPLMICHNCGGRGHLAYEC 110
>TC11804 similar to GB|AAO63413.1|28950979|BT005349 At5g01420 {Arabidopsis
thaliana;}, partial (12%)
Length = 548
Score = 33.1 bits (74), Expect = 0.053
Identities = 25/97 (25%), Positives = 40/97 (40%), Gaps = 2/97 (2%)
Frame = +3
Query: 145 VWLVLIGRSMGYRMVDLNRMAEATREHHQRLMATAMYQQRGMNRTGAGGPMRKFGHFANK 204
+W +L G+++ R+ R E +++Q + + G PM F +
Sbjct: 9 LWSILDGKTLPPRLFVKGRYIGGAEE------VVTLHEQGKLKKLFEGVPMDYFDGACDA 170
Query: 205 CS-VTGWRCFKCNREGHLAVNCKTKESL-CFNCNQPG 239
C V CFKCN + + KES C CN+ G
Sbjct: 171 CGGVRFVLCFKCNGSHKVIAENEEKESTQCPQCNENG 281
>BP033819
Length = 554
Score = 32.3 bits (72), Expect = 0.090
Identities = 14/28 (50%), Positives = 19/28 (67%)
Frame = -2
Query: 37 DEDPDFMADRGRVDNSSVGYGWLESFEL 64
D DPD M D+ R D+ S+GYG LE ++
Sbjct: 553 DVDPDRMHDQMRFDDGSIGYGCLEPIKV 470
>AV421607
Length = 245
Score = 30.8 bits (68), Expect = 0.26
Identities = 11/33 (33%), Positives = 17/33 (51%)
Frame = +3
Query: 232 CFNCNQPGHFSQDCMALRGESSGNVGKGKQLAP 264
C+ C +PGH+S+DC + S+ N P
Sbjct: 54 CYKCGRPGHWSRDCPSSAPNSNPNPNPNTTTTP 152
>AV773507
Length = 496
Score = 30.0 bits (66), Expect = 0.45
Identities = 15/42 (35%), Positives = 19/42 (44%)
Frame = +2
Query: 232 CFNCNQPGHFSQDCMALRGESSGNVGKGKQLAPEERIHAKEG 273
CF C +PGH ++DC L G G G L+ R G
Sbjct: 350 CFECGRPGHRARDC-PLAGGGGGRGRGGGSLSSRPRYGGSGG 472
>BP039068
Length = 467
Score = 29.6 bits (65), Expect = 0.58
Identities = 9/21 (42%), Positives = 15/21 (70%)
Frame = +3
Query: 228 KESLCFNCNQPGHFSQDCMAL 248
++++C NC + GH S DC +L
Sbjct: 300 RQTVCMNCQETGHASNDCPSL 362
>TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (89%)
Length = 912
Score = 29.6 bits (65), Expect = 0.58
Identities = 10/21 (47%), Positives = 16/21 (75%)
Frame = +1
Query: 232 CFNCNQPGHFSQDCMALRGES 252
C+ C +PGHF+++C +RG S
Sbjct: 379 CYECGEPGHFAREC-RMRGGS 438
Score = 27.3 bits (59), Expect = 2.9
Identities = 20/88 (22%), Positives = 32/88 (35%), Gaps = 3/88 (3%)
Frame = +1
Query: 144 TVWLVLIGRSMGYRMVDLNRMAEAT---REHHQRLMATAMYQQRGMNRTGAGGPMRKFGH 200
+VW+ R GY +D + +A RE + G GG R G
Sbjct: 172 SVWVAR--RPPGYAFIDFDDRRDAQDAIRELDGKNGWRVELSHNSRGGGGGGGGGRGGGR 345
Query: 201 FANKCSVTGWRCFKCNREGHLAVNCKTK 228
+ +C++C GH A C+ +
Sbjct: 346 SGGGGGGSDMKCYECGEPGHFARECRMR 429
>BP079571
Length = 414
Score = 29.6 bits (65), Expect = 0.58
Identities = 13/32 (40%), Positives = 17/32 (52%)
Frame = -3
Query: 210 WRCFKCNREGHLAVNCKTKESLCFNCNQPGHF 241
WRC H+ V E+ CF+C +PGHF
Sbjct: 310 WRCC------HVVVVGMLGETHCFHCGKPGHF 233
>BP033671
Length = 482
Score = 29.3 bits (64), Expect = 0.76
Identities = 13/15 (86%), Positives = 13/15 (86%)
Frame = +3
Query: 83 KSGCYIVVSELSRVF 97
KSGCYIVVSE RVF
Sbjct: 438 KSGCYIVVSEHGRVF 482
>TC12773
Length = 420
Score = 28.9 bits (63), Expect = 0.99
Identities = 9/22 (40%), Positives = 16/22 (71%)
Frame = +2
Query: 204 KCSVTGWRCFKCNREGHLAVNC 225
+ S++ + C+KC R GH+A +C
Sbjct: 323 RSSLSTYECWKCQRPGHMAEDC 388
Score = 28.1 bits (61), Expect = 1.7
Identities = 7/15 (46%), Positives = 12/15 (79%)
Frame = +2
Query: 232 CFNCNQPGHFSQDCM 246
C+ C +PGH ++DC+
Sbjct: 347 CWKCQRPGHMAEDCL 391
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.324 0.136 0.427
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,426,790
Number of Sequences: 28460
Number of extensions: 78520
Number of successful extensions: 530
Number of sequences better than 10.0: 54
Number of HSP's better than 10.0 without gapping: 502
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 525
length of query: 275
length of database: 4,897,600
effective HSP length: 89
effective length of query: 186
effective length of database: 2,364,660
effective search space: 439826760
effective search space used: 439826760
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0103.5