BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0103.4
(378 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8970 58 6e-12
TC8994 homologue to UP|RS0_NEUCR (Q01291) 40S ribosomal protein ... 43 2e-09
TC8995 51 4e-07
AV408058 32 7e-05
BP052706 38 0.003
TC11354 similar to UP|CAE54480 (CAE54480) Alpha glucosidase II ... 37 0.004
AV776568 34 0.046
TC16761 similar to GB|AAL32703.1|17065098|AY062625 nucleotide su... 33 0.078
TC15384 weakly similar to UP|Q9LVB8 (Q9LVB8) HSR203J protein-lik... 33 0.10
TC8243 similar to GB|AAP21170.1|30102504|BT006362 At3g19170/MVI1... 33 0.10
TC16823 similar to UP|Q8LGA2 (Q8LGA2) Seed maturation-like prote... 32 0.13
BP033819 32 0.13
TC9115 similar to PIR|T00425|T00425 photolyase/blue-light recept... 32 0.13
TC11700 homologue to GB|AAL06971.1|15810091|AY056083 AT4g30260/F... 32 0.13
TC16697 32 0.13
TC17147 similar to UP|Q8LP14 (Q8LP14) Nine-cis-epoxycarotenoid d... 32 0.17
TC16847 weakly similar to PIR|T51733|T51733 cytidine deaminase ... 32 0.23
TC14842 similar to UP|RK4_TOBAC (O80361) 50S ribosomal protein L... 32 0.23
AV769326 31 0.30
TC9116 similar to UP|Q9ZW85 (Q9ZW85) 3-isopropylmalate dehydrata... 31 0.30
>TC8970
Length = 609
Score = 57.8 bits (138), Expect(2) = 6e-12
Identities = 30/46 (65%), Positives = 35/46 (75%), Gaps = 1/46 (2%)
Frame = +1
Query: 180 AFDEDPDFMADRGRVDNSSVGYGWLESFELLVTVIALI-RLTFGTG 224
AFD DPDFM D+ RV++S VGYGWLE L+V VIALI +L FG G
Sbjct: 145 AFDVDPDFMEDQMRVEDSGVGYGWLELLILVVMVIALIHQLYFGAG 282
Score = 28.9 bits (63), Expect(2) = 6e-12
Identities = 15/38 (39%), Positives = 22/38 (57%)
Frame = +2
Query: 142 GTVYLYTFGYSIYLRASVSRFVMIRCAGKTRSEMSITE 179
G V ++ FG S + F +R +G+TRSEM+ TE
Sbjct: 32 GFVCMFVFGRSACCQTFNR*FKKVRRSGRTRSEMTTTE 145
>TC8994 homologue to UP|RS0_NEUCR (Q01291) 40S ribosomal protein S0
(Ribosome-associated protein 1), partial (6%)
Length = 1009
Score = 43.1 bits (100), Expect(2) = 2e-09
Identities = 20/28 (71%), Positives = 23/28 (81%)
Frame = -2
Query: 327 LQGNLLVNQSNHLLPATNNYSQHYLLKP 354
LQ NLLVNQSNHLL A N+YS+ +LL P
Sbjct: 444 LQANLLVNQSNHLLAAINSYSKQHLLGP 361
Score = 35.0 bits (79), Expect(2) = 2e-09
Identities = 15/31 (48%), Positives = 23/31 (73%)
Frame = -3
Query: 297 SILPTVPAMENCKIAMHRNPPKSQVNYTTIL 327
+ILPT+P++E + MHRN PK++V+ T L
Sbjct: 629 NILPTIPSLEIRRRVMHRNLPKTKVSITIFL 537
>TC8995
Length = 560
Score = 50.8 bits (120), Expect = 4e-07
Identities = 32/90 (35%), Positives = 46/90 (50%), Gaps = 32/90 (35%)
Frame = -3
Query: 297 SILPTVPAMENCKIAMHRNPPKSQVNYTTI------------------------------ 326
+ILPT+P++E + MHRN PK++V+ T I
Sbjct: 324 NILPTIPSLEIRRRVMHRNLPKTKVSNTIIPTSFNQ*R*ASLPKTLALHLTITSTSLRLV 145
Query: 327 --LQGNLLVNQSNHLLPATNNYSQHYLLKP 354
LQG+L+VN+SNHLL A N+ S+ +LL P
Sbjct: 144 HSLQGSLIVNKSNHLLAAINSSSETHLLGP 55
>AV408058
Length = 386
Score = 31.6 bits (70), Expect(2) = 7e-05
Identities = 14/23 (60%), Positives = 17/23 (73%)
Frame = +3
Query: 180 AFDEDPDFMADRGRVDNSSVGYG 202
AFD DPDFM D+ VD+ S+G G
Sbjct: 318 AFDVDPDFMHDQRGVDDGSMGDG 386
Score = 30.8 bits (68), Expect(2) = 7e-05
Identities = 14/32 (43%), Positives = 22/32 (68%)
Frame = +1
Query: 148 TFGYSIYLRASVSRFVMIRCAGKTRSEMSITE 179
+ G + +S+ FV++R +GKTRSEM+ TE
Sbjct: 223 SLGRKLAKMSSMDSFVILRHSGKTRSEMTTTE 318
>BP052706
Length = 503
Score = 37.7 bits (86), Expect = 0.003
Identities = 25/54 (46%), Positives = 32/54 (58%), Gaps = 2/54 (3%)
Frame = -2
Query: 144 VYLYTFGYSIYLRASVSRFVMIRCAGKTRSEMSITEAFDEDPDFMA--DRGRVD 195
VYL+ FG S + + FVM+R +G+ RSEM E DED FM+ D GR D
Sbjct: 364 VYLFVFGRSXNCKMVNNWFVMVRHSGQVRSEM--IEHSDEDSYFMSEKDAGR*D 209
>TC11354 similar to UP|CAE54480 (CAE54480) Alpha glucosidase II , partial
(6%)
Length = 583
Score = 37.4 bits (85), Expect = 0.004
Identities = 33/101 (32%), Positives = 40/101 (38%), Gaps = 1/101 (0%)
Frame = -1
Query: 223 TGVRPPISQHNKKVSRLGSNPMNKLDSPPKTDSIITPNTRLKIHVPPRPHPALASPSLAS 282
T PP S N NP + T S +P+T K RP PA SP+ +S
Sbjct: 310 TATSPPTSSPNTHPPTPTPNP*SSPSPSTTTASSASPST--KTLPSTRPRPASESPTSSS 137
Query: 283 STLADPRSGPSVT-QSILPTVPAMENCKIAMHRNPPKSQVN 322
+ P SG SV+ I P P N A PP S N
Sbjct: 136 PSSPPPSSGSSVSPPRISPPPPPPSNSPTA---TPPSSATN 23
>AV776568
Length = 628
Score = 33.9 bits (76), Expect = 0.046
Identities = 24/115 (20%), Positives = 42/115 (35%), Gaps = 1/115 (0%)
Frame = +1
Query: 243 PMNKLDSPPKTDSIITPNTRLKIHVPPRPHPALASPSLASSTLADPRSGPSVTQSILPTV 302
P + PP + R P P+ ASP S + P P++ + L T
Sbjct: 184 PRRAMSKPPSAFDALMSGARAAAAKKKPPPPSAASPKKRKSPHSSPAPAPTLNPNNLKTP 363
Query: 303 PAMENCKIAMHRNPPKSQVNYTTILQ-GNLLVNQSNHLLPATNNYSQHYLLKPGK 356
++ + ++ P S+ T+LQ + N S L+ + KP +
Sbjct: 364 ETVQPPRDSVETVQPSSEEAVETVLQPAKKICNSSGSLIAELKELAPRLKKKPSE 528
>TC16761 similar to GB|AAL32703.1|17065098|AY062625 nucleotide sugar
epimerase-like protein {Arabidopsis thaliana;}, partial
(40%)
Length = 794
Score = 33.1 bits (74), Expect = 0.078
Identities = 23/78 (29%), Positives = 29/78 (36%)
Frame = +1
Query: 227 PPISQHNKKVSRLGSNPMNKLDSPPKTDSIITPNTRLKIHVPPRPHPALASPSLASSTLA 286
PP S H+ S S+P L SPP + TP PH A P + S
Sbjct: 286 PPSSSHSSSSS---SSPSTTLPSPPTPPTTSTPTP-----TSSPPHSAAEPPGRSKSATP 441
Query: 287 DPRSGPSVTQSILPTVPA 304
P + P+ S P A
Sbjct: 442 PPHAAPTACPSSSPAQSA 495
>TC15384 weakly similar to UP|Q9LVB8 (Q9LVB8) HSR203J protein-like protein,
partial (21%)
Length = 1193
Score = 32.7 bits (73), Expect = 0.10
Identities = 19/53 (35%), Positives = 32/53 (59%)
Frame = -2
Query: 266 HVPPRPHPALASPSLASSTLADPRSGPSVTQSILPTVPAMENCKIAMHRNPPK 318
+VP HP AS S A LADP +G ++S++ ++ A+ + I+ R+PP+
Sbjct: 967 NVPQLSHPPSASTSPAP--LADP*AGHPNSRSLISSIQAV*SLPISQDRSPPQ 815
>TC8243 similar to GB|AAP21170.1|30102504|BT006362 At3g19170/MVI11_8
{Arabidopsis thaliana;}, partial (13%)
Length = 613
Score = 32.7 bits (73), Expect = 0.10
Identities = 25/89 (28%), Positives = 40/89 (44%), Gaps = 21/89 (23%)
Frame = -3
Query: 12 DRTPFIINCLLSPNRH---IPSGTVYLYTFGYSIYL-----------------RVQYIYI 51
+ PFI+NC N+ + + YL+T +L ++ +YI
Sbjct: 353 NHNPFILNCFHCINKISEVLHTSR*YLFTSSLPFFLCDTQ*VPQQTTITLGIRKLI*VYI 174
Query: 52 PSGTVYTFGYSISIYLRVQ-YIPSGTVYL 79
P+G+ Y FG SI IY + IPS +Y+
Sbjct: 173 PNGSNYGFG*SIIIYFQFS*EIPSSIIYI 87
>TC16823 similar to UP|Q8LGA2 (Q8LGA2) Seed maturation-like protein, partial
(29%)
Length = 711
Score = 32.3 bits (72), Expect = 0.13
Identities = 28/93 (30%), Positives = 42/93 (45%), Gaps = 13/93 (13%)
Frame = +1
Query: 224 GVRPPISQHNKKVSRLGSNPMNKLDSPPK------TDSIITPNTRLKIHVPP-----RPH 272
G R P+ H +S NP + SP + ITP+++ PP RP
Sbjct: 175 GSRRPLLPHPLTMSSPPGNPPSTAASPSSPTCSAASSPSITPSSQRVSPTPPETP*SRPS 354
Query: 273 P--ALASPSLASSTLADPRSGPSVTQSILPTVP 303
P ++ SP + S +L+ S PS+ S LP+ P
Sbjct: 355 PPCSVCSPPITSLSLSLFPSTPSIVFSSLPSSP 453
>BP033819
Length = 554
Score = 32.3 bits (72), Expect = 0.13
Identities = 14/28 (50%), Positives = 19/28 (67%)
Frame = -2
Query: 182 DEDPDFMADRGRVDNSSVGYGWLESFEL 209
D DPD M D+ R D+ S+GYG LE ++
Sbjct: 553 DVDPDRMHDQMRFDDGSIGYGCLEPIKV 470
>TC9115 similar to PIR|T00425|T00425 photolyase/blue-light receptor (PHR2)
[imported] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (55%)
Length = 1018
Score = 32.3 bits (72), Expect = 0.13
Identities = 28/105 (26%), Positives = 41/105 (38%), Gaps = 1/105 (0%)
Frame = +1
Query: 248 DSPPKTDSIITPNTRLKIHVPPRPHPALASPSLASSTLADPRSGPSVTQSILPTVP-AME 306
++P ++ S P H PP PHP +P ++P S P+ + + P P A
Sbjct: 235 NNPTRSKSQPKPPPSPTSHSPPPPHPRRPNPP------SNPPSPPTPSAPLTP*APTAPS 396
Query: 307 NCKIAMHRNPPKSQVNYTTILQGNLLVNQSNHLLPATNNYSQHYL 351
H P S + TT ++ P T N SQ YL
Sbjct: 397 TLPTPPHSAAPPSSGSATTSASATTRLS-----TPPTTNRSQFYL 516
>TC11700 homologue to GB|AAL06971.1|15810091|AY056083 AT4g30260/F9N11_110
{Arabidopsis thaliana;}, partial (45%)
Length = 561
Score = 32.3 bits (72), Expect = 0.13
Identities = 26/85 (30%), Positives = 38/85 (44%), Gaps = 3/85 (3%)
Frame = +3
Query: 223 TGVRPPISQHNKKVSRLGSNPMNKLDSPPKTDSIITPNTRLKIHVPPRPHPALASPSLAS 282
T +RPP S + V +P SPP S I + L + PP P+ +PSL+
Sbjct: 105 THLRPPSSPPDHPVLHAPPSP-----SPPLLPSSIPTSLLLFLPNPPSPNLNFQNPSLSL 269
Query: 283 STL---ADPRSGPSVTQSILPTVPA 304
L DP+S P + +L P+
Sbjct: 270 LLLLPPPDPKSPPPASDPLLIPSPS 344
>TC16697
Length = 635
Score = 32.3 bits (72), Expect = 0.13
Identities = 21/78 (26%), Positives = 33/78 (41%), Gaps = 3/78 (3%)
Frame = -1
Query: 228 PISQHNKKVSRLGSNPMNKLDSPPKTDSIITPNTRLKIHVPPRPHPALASPSLAS---ST 284
P + +K + + P N SPP S P + P+PHP+ SP+ +S S
Sbjct: 536 PPAPSQRKPAPATAPPSNSSQSPPPNPSSTDPTSPA-----PQPHPSHLSPASSSSQGSP 372
Query: 285 LADPRSGPSVTQSILPTV 302
+ P P +T P +
Sbjct: 371 VPSPSPSPPITSPSSPPI 318
>TC17147 similar to UP|Q8LP14 (Q8LP14) Nine-cis-epoxycarotenoid
dioxygenase4, partial (22%)
Length = 663
Score = 32.0 bits (71), Expect = 0.17
Identities = 17/57 (29%), Positives = 25/57 (43%)
Frame = -1
Query: 232 HNKKVSRLGSNPMNKLDSPPKTDSIITPNTRLKIHVPPRPHPALASPSLASSTLADP 288
H++ V + P ++ PP+ + T + K PP PH A SPS L P
Sbjct: 357 HSQTVHETRTAPSSEASPPPQPPAPATEHPSPKTSTPPSPHHARNSPSTHHPPLPPP 187
Score = 26.9 bits (58), Expect = 5.6
Identities = 18/79 (22%), Positives = 28/79 (34%)
Frame = -1
Query: 227 PPISQHNKKVSRLGSNPMNKLDSPPKTDSIITPNTRLKIHVPPRPHPALASPSLASSTLA 286
PP H + P+ P + + N R + PP PH S S+ +
Sbjct: 249 PPSPHHARNSPSTHHPPLPPPPPPTQKTARRRSNPRRTSYTPPSPHSPRQSSPPQKSSAS 70
Query: 287 DPRSGPSVTQSILPTVPAM 305
DP + T S P + +
Sbjct: 69 DPPPPRTQTCSSSPRISGL 13
>TC16847 weakly similar to PIR|T51733|T51733 cytidine deaminase [validated]
- Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (74%)
Length = 1098
Score = 31.6 bits (70), Expect = 0.23
Identities = 22/71 (30%), Positives = 33/71 (45%), Gaps = 1/71 (1%)
Frame = +1
Query: 249 SPPKTDSIITPNTRLKIHVPPRPHPALASPSLASSTLADPRSGPSVTQSIL-PTVPAMEN 307
+PPK S + P+ + P PA +S + SS+ A P + PS S PT P+M
Sbjct: 133 APPKA-SPVPPSPNSR*QRWPSAPPAASSSASTSSSPASPSTTPSTPNSFS*PTSPSMRR 309
Query: 308 CKIAMHRNPPK 318
+PP+
Sbjct: 310 LSYGGSPSPPR 342
>TC14842 similar to UP|RK4_TOBAC (O80361) 50S ribosomal protein L4,
chloroplast precursor (R-protein L4), partial (51%)
Length = 618
Score = 31.6 bits (70), Expect = 0.23
Identities = 28/95 (29%), Positives = 40/95 (41%), Gaps = 15/95 (15%)
Frame = +1
Query: 225 VRPPI-SQHNKKVSRLGSNPMNKLDSPPKTD-------------SIITPNTRLKIHVPPR 270
++PP S + + SR +P K SPP T + +P ++ PP
Sbjct: 175 IQPPSPSPASSQPSRFSPSPARKSASPPSTSKPPHQTPHVPSSTAPSSPTSKTSAAAPPP 354
Query: 271 PHPALASPSLA-SSTLADPRSGPSVTQSILPTVPA 304
P PA S ++A S TL P Q+ L VPA
Sbjct: 355 PRPARRSAAVARSPTLRRRPVAPGEGQTGLRFVPA 459
Score = 26.9 bits (58), Expect = 5.6
Identities = 19/65 (29%), Positives = 24/65 (36%), Gaps = 11/65 (16%)
Frame = +1
Query: 258 TPNTRLKIHVPPRPHPALASPSLAS-----------STLADPRSGPSVTQSILPTVPAME 306
+P L + PP P PA + PS S ST P P V S P+ P +
Sbjct: 151 SPQNSLILIQPPSPSPASSQPSRFSPSPARKSASPPSTSKPPHQTPHVPSSTAPSSPTSK 330
Query: 307 NCKIA 311
A
Sbjct: 331 TSAAA 345
>AV769326
Length = 262
Score = 31.2 bits (69), Expect = 0.30
Identities = 23/64 (35%), Positives = 31/64 (47%), Gaps = 8/64 (12%)
Frame = +2
Query: 249 SPPKTDSIITPNTRLKIHVPPRPHPALAS----PSLASSTLADPRSGPSVTQ----SILP 300
SPP T TP+ H PP P+ AS PSL + +L P S P+ T+ S P
Sbjct: 53 SPPSTTPT-TPSPPSDPHSPPSNPPSAASDPNSPSLTAPSLQKPPSSPTSTEPPSSSSTP 229
Query: 301 TVPA 304
+ P+
Sbjct: 230 SAPS 241
>TC9116 similar to UP|Q9ZW85 (Q9ZW85) 3-isopropylmalate dehydratase, small
subunit, partial (25%)
Length = 426
Score = 31.2 bits (69), Expect = 0.30
Identities = 15/34 (44%), Positives = 23/34 (67%), Gaps = 1/34 (2%)
Frame = +1
Query: 269 PRPHPALASPSLASSTLADPRSG-PSVTQSILPT 301
P+PHP+ AS + +++T RS PS++ S LPT
Sbjct: 220 PQPHPSTASATSSATTSTPTRSSPPSISPSSLPT 321
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.138 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,850,144
Number of Sequences: 28460
Number of extensions: 112784
Number of successful extensions: 970
Number of sequences better than 10.0: 110
Number of HSP's better than 10.0 without gapping: 849
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 955
length of query: 378
length of database: 4,897,600
effective HSP length: 92
effective length of query: 286
effective length of database: 2,279,280
effective search space: 651874080
effective search space used: 651874080
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0103.4