BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0103.12
(1541 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC12658 similar to UP|Q9SSE9 (Q9SSE9) MLP3.6 protein, partial (16%) 252 3e-67
CB828083 146 2e-35
BP039096 99 4e-21
TC17921 similar to UP|O04698 (O04698) Chloroplast DNA-binding pr... 78 1e-14
TC9529 similar to UP|O48794 (O48794) F24O1.3, partial (9%) 73 4e-13
BP063052 62 9e-10
TC9996 61 1e-09
BP028413 43 4e-04
TC18947 homologue to GB|AAM19932.1|20453387|AY097416 AT4g17800/d... 34 0.20
BI419395 34 0.20
BP081200 33 0.26
TC17587 similar to UP|HIS5_ARATH (Q9SZ30) Imidazole glycerol pho... 33 0.45
AV409843 33 0.45
AV419982 32 0.76
TC15800 homologue to GB|AAM19932.1|20453387|AY097416 AT4g17800/d... 32 1.00
TC12104 32 1.00
TC17986 similar to GB|AAP21231.1|30102626|BT006423 At4g14465 {Ar... 31 1.3
BP038715 30 2.9
TC11910 similar to GB|CAA54498.1|602891|SCIILDNA YBL0422 {Saccha... 30 2.9
TC10673 homologue to UP|Q8S8Z0 (Q8S8Z0) Protein phosphatase 2C, ... 28 8.5
>TC12658 similar to UP|Q9SSE9 (Q9SSE9) MLP3.6 protein, partial (16%)
Length = 595
Score = 252 bits (644), Expect = 3e-67
Identities = 120/183 (65%), Positives = 137/183 (74%), Gaps = 1/183 (0%)
Frame = +2
Query: 1076 EYEHFQRHWIRGEPVIVRNVFEKASGLSWHPMVMWRAFRGANKILKEEPTTFKAIDCLDW 1135
++ HFQ HW +GEPVIV NV E ASGLSW P+VMWRAFR K + KAIDCLDW
Sbjct: 11 DFRHFQLHWSKGEPVIVSNVLEHASGLSWEPLVMWRAFRQMFKTKHDRHLDVKAIDCLDW 190
Query: 1136 CEVQINIFQFFKGYLEGRRYRNGWPEMLKLKDWPPSNSFEECFPRHGAEFIAMLPFSDYT 1195
CE +INI QFF GY GR WP++LKLKDWPPSN FEE PRH AEFI+ LPF +YT
Sbjct: 191 CEGEINIHQFFTGYTNGRDDWLDWPQILKLKDWPPSNLFEERLPRHCAEFISSLPFKEYT 370
Query: 1196 HPKFGILNLATKLP-AVLKPDLGPKTYIAYGSLEELSRGDSVTKLHCDISDAVNILTHTE 1254
+P G LNLA KLP +KPD+GPKTYIAYG +EL RGDSVTKLHCD+SDAVN+LTH
Sbjct: 371 NPYKGALNLAVKLPKGCIKPDMGPKTYIAYGFAQELGRGDSVTKLHCDMSDAVNVLTHVA 550
Query: 1255 EVK 1257
EV+
Sbjct: 551 EVQ 559
>CB828083
Length = 514
Score = 146 bits (369), Expect = 2e-35
Identities = 65/88 (73%), Positives = 73/88 (82%)
Frame = +1
Query: 1453 GSAVWDIFRRQDVPKLTEYLKKHHKEFRHANNLPVDSVTHPIHDQILYLNEKHKRQLKKE 1512
G A+WDIFRRQDVPKL EYLKKH +EFRH + P+ V HPIHDQ YL +HKR+LK+E
Sbjct: 100 GGALWDIFRRQDVPKLQEYLKKHFREFRHVHCSPLTKVVHPIHDQTFYLTMEHKRKLKEE 279
Query: 1513 YGIEPWTFEQHLGEAVFIPAGCPHQVRN 1540
YGIEPWTF Q LG+AVFIPAGCPHQVRN
Sbjct: 280 YGIEPWTFVQKLGDAVFIPAGCPHQVRN 363
>BP039096
Length = 492
Score = 99.4 bits (246), Expect = 4e-21
Identities = 47/118 (39%), Positives = 62/118 (51%)
Frame = +2
Query: 800 CHQCWRNSWSGVVICAKCKRKQYCYECITKWYPGKTREEIEIACPFCLRNCNCRLCLKKD 859
CHQC + + V C KC K YC C+ +WYP + EE+ +CPFC +NCNC CL
Sbjct: 134 CHQCMKKERTAFVPCTKCP-KMYCMRCLHQWYPDMSIEEVAGSCPFCRKNCNCNACLHSK 310
Query: 860 ISVMTGSGEADTGVILQKLLYLLNKTLPLLQHIQREQISEMEVEASMHGSPLMEEDIQ 917
+ T + Q L Y++N LP L+ I EQ E E+EA + G E DIQ
Sbjct: 311 GVIKTSDNDIANSDRAQYLHYMINLLLPYLKQICHEQTQEKEIEAKIQGISSCEIDIQ 484
>TC17921 similar to UP|O04698 (O04698) Chloroplast DNA-binding protein PD3,
partial (6%)
Length = 506
Score = 77.8 bits (190), Expect = 1e-14
Identities = 33/42 (78%), Positives = 38/42 (89%)
Frame = +2
Query: 1500 YLNEKHKRQLKKEYGIEPWTFEQHLGEAVFIPAGCPHQVRNR 1541
+LNE+HK+QLK+E+ IEPWTFE LGEAVFIPAGCPH VRNR
Sbjct: 2 FLNERHKKQLKREFHIEPWTFEPPLGEAVFIPAGCPHPVRNR 127
>TC9529 similar to UP|O48794 (O48794) F24O1.3, partial (9%)
Length = 614
Score = 72.8 bits (177), Expect = 4e-13
Identities = 29/41 (70%), Positives = 37/41 (89%)
Frame = +1
Query: 1500 YLNEKHKRQLKKEYGIEPWTFEQHLGEAVFIPAGCPHQVRN 1540
YL +H ++LK+E+G+EPWTF+Q+LGE VFIPAGCPHQVRN
Sbjct: 7 YLTWEHXKKLKEEFGVEPWTFKQNLGEXVFIPAGCPHQVRN 129
>BP063052
Length = 485
Score = 61.6 bits (148), Expect = 9e-10
Identities = 24/33 (72%), Positives = 31/33 (93%)
Frame = -1
Query: 1508 QLKKEYGIEPWTFEQHLGEAVFIPAGCPHQVRN 1540
+LK+E+ IEPWTF+QH+GEAV IPAGCP+Q+RN
Sbjct: 482 RLKEEFKIEPWTFQQHVGEAVIIPAGCPYQIRN 384
>TC9996
Length = 586
Score = 61.2 bits (147), Expect = 1e-09
Identities = 25/33 (75%), Positives = 30/33 (90%)
Frame = +2
Query: 1508 QLKKEYGIEPWTFEQHLGEAVFIPAGCPHQVRN 1540
+LK+E+ IEPWTFEQH GEAV IPAGCP+Q+RN
Sbjct: 2 RLKEEFEIEPWTFEQHAGEAVIIPAGCPYQMRN 100
>BP028413
Length = 528
Score = 42.7 bits (99), Expect = 4e-04
Identities = 18/18 (100%), Positives = 18/18 (100%)
Frame = -3
Query: 1524 LGEAVFIPAGCPHQVRNR 1541
LGEAVFIPAGCPHQVRNR
Sbjct: 526 LGEAVFIPAGCPHQVRNR 473
>TC18947 homologue to GB|AAM19932.1|20453387|AY097416 AT4g17800/dl4935c
{Arabidopsis thaliana;}, partial (25%)
Length = 435
Score = 33.9 bits (76), Expect = 0.20
Identities = 26/91 (28%), Positives = 44/91 (47%), Gaps = 4/91 (4%)
Frame = +3
Query: 475 NKVNNIMEVSKKAASGGDCKIAGPKKCGRPKGSKNKQKNIVQVSQEVAGSADCEI----A 530
N N ++++ + GD + G + GRP GSKNK K V +++E A + I +
Sbjct: 174 NNSNEGLDLATQNQGPGD--VVGRRPRGRPPGSKNKPKPPVIITRESANTLRAHILEVSS 347
Query: 531 GPKKCGRPKGSMKKRKSLVCASILEGAGGIT 561
G +KR+ +C +L G+G +T
Sbjct: 348 GCDVFDSVATYARKRQKGIC--VLSGSGTVT 434
Score = 33.9 bits (76), Expect = 0.20
Identities = 15/47 (31%), Positives = 27/47 (56%)
Frame = +3
Query: 443 NKLKNTVDCNNEGSGAGEIVRPKKRGRPRGSKNKVNNIMEVSKKAAS 489
N +D + G G++V + RGRP GSKNK + +++++A+
Sbjct: 174 NNSNEGLDLATQNQGPGDVVGRRPRGRPPGSKNKPKPPVIITRESAN 314
>BI419395
Length = 588
Score = 33.9 bits (76), Expect = 0.20
Identities = 13/29 (44%), Positives = 16/29 (54%)
Frame = -3
Query: 9 RRCGRKAPPGWRCTERALSGKSVCERHFL 37
R C +P WRC R ++ K VCE H L
Sbjct: 523 RGCAGASPVKWRCRRRVMANKKVCEIHHL 437
>BP081200
Length = 427
Score = 33.5 bits (75), Expect = 0.26
Identities = 24/81 (29%), Positives = 38/81 (46%), Gaps = 4/81 (4%)
Frame = +1
Query: 489 SGGDCKIAGPKKCGRPKGSKNKQKNIVQVSQEVAGSADCEI----AGPKKCGRPKGSMKK 544
S G + G + GRP GSKNK K V +++E A + I +G ++
Sbjct: 130 SAGPGDVVGRRPRGRPPGSKNKPKPPVIITRESANTLRAHILEVASGSDVFDCVTAYARR 309
Query: 545 RKSLVCASILEGAGGITREGL 565
R+ +C +L G+G +T L
Sbjct: 310 RQRGIC--VLSGSGTVTNVSL 366
Score = 31.2 bits (69), Expect = 1.3
Identities = 13/34 (38%), Positives = 24/34 (70%)
Frame = +1
Query: 456 SGAGEIVRPKKRGRPRGSKNKVNNIMEVSKKAAS 489
+G G++V + RGRP GSKNK + +++++A+
Sbjct: 133 AGPGDVVGRRPRGRPPGSKNKPKPPVIITRESAN 234
>TC17587 similar to UP|HIS5_ARATH (Q9SZ30) Imidazole glycerol phosphate
synthase hisHF, chloroplast precursor (IGP synthase)
(ImGP synthase) (IGPS) [Includes: Glutamine
amidotransferase ; Cyclase ] , partial (12%)
Length = 431
Score = 32.7 bits (73), Expect = 0.45
Identities = 23/67 (34%), Positives = 31/67 (45%)
Frame = -1
Query: 927 NFHRSCPNPNCRYDLCLTCCMELRNGLHYEDIPASGNEETIDEPPITSAWRAEINGRIPC 986
+FH S + N R D C + GLH D+ A G++ D P I S + + RIP
Sbjct: 320 HFHGSSKSSNTRKD*S-ACIQNVLWGLHIFDLKAEGSDSIPDTPNIASTIVKQRHNRIP- 147
Query: 987 PPKARGG 993
RGG
Sbjct: 146 ---RRGG 135
>AV409843
Length = 414
Score = 32.7 bits (73), Expect = 0.45
Identities = 23/67 (34%), Positives = 31/67 (45%)
Frame = -3
Query: 927 NFHRSCPNPNCRYDLCLTCCMELRNGLHYEDIPASGNEETIDEPPITSAWRAEINGRIPC 986
+FH S + N R D C + GLH D+ A G++ D P I S + + RIP
Sbjct: 328 HFHGSSKSSNTRKD*S-ACIQNVLWGLHIFDLKAEGSDSIPDTPNIASTIVKQRHNRIP- 155
Query: 987 PPKARGG 993
RGG
Sbjct: 154 ---RRGG 143
>AV419982
Length = 415
Score = 32.0 bits (71), Expect = 0.76
Identities = 12/27 (44%), Positives = 16/27 (58%)
Frame = -1
Query: 1078 EHFQRHWIRGEPVIVRNVFEKASGLSW 1104
E FQ+ W+ GE +V F+K GL W
Sbjct: 415 ERFQKRWVLGESFLVPCFFQKMKGLMW 335
>TC15800 homologue to GB|AAM19932.1|20453387|AY097416 AT4g17800/dl4935c
{Arabidopsis thaliana;}, partial (39%)
Length = 818
Score = 31.6 bits (70), Expect = 1.00
Identities = 26/108 (24%), Positives = 50/108 (46%), Gaps = 4/108 (3%)
Frame = +1
Query: 462 VRPKKRGRPRGSKNKVNNIMEVSKKAASGGDCKIAGPKKCGRPKGSKNKQKNIVQVSQEV 521
++ +++ +P+ S+ + N+ + + G + GRP GSKNKQK V +++E
Sbjct: 379 LQQQQQQQPQESEEEPNHNSDPFSAEDGPRSGDLVGRRPRGRPLGSKNKQKPPVIITRES 558
Query: 522 AGSADCEIA----GPKKCGRPKGSMKKRKSLVCASILEGAGGITREGL 565
A + I G ++R+ +C +L G+G +T L
Sbjct: 559 ANTLRAHILEVGNGCDVFDCVATYARRRQRGIC--VLSGSGTVTNVSL 696
Score = 30.8 bits (68), Expect = 1.7
Identities = 13/36 (36%), Positives = 25/36 (69%)
Frame = +1
Query: 454 EGSGAGEIVRPKKRGRPRGSKNKVNNIMEVSKKAAS 489
+G +G++V + RGRP GSKNK + +++++A+
Sbjct: 457 DGPRSGDLVGRRPRGRPLGSKNKQKPPVIITRESAN 564
>TC12104
Length = 771
Score = 31.6 bits (70), Expect = 1.00
Identities = 14/47 (29%), Positives = 22/47 (46%)
Frame = +1
Query: 800 CHQCWRNSWSGVVICAKCKRKQYCYECITKWYPGKTREEIEIACPFC 846
C C S +++C K +C EC+++W+ E E CP C
Sbjct: 421 CAICQEKMHSPILLCCK---HMFCEECVSEWF------ERERTCPLC 534
>TC17986 similar to GB|AAP21231.1|30102626|BT006423 At4g14465 {Arabidopsis
thaliana;}, partial (49%)
Length = 628
Score = 31.2 bits (69), Expect = 1.3
Identities = 50/213 (23%), Positives = 76/213 (35%), Gaps = 20/213 (9%)
Frame = +1
Query: 409 KSSKCRKKNIMEAG-------DEAAGEIGGDKKLGRPKGSLNKLKNTVDCNNEGSGAGEI 461
K K +KK+ + A G G D PK S+N GSG+ I
Sbjct: 16 KKRKKKKKHYLSATLANPWWTGHQVGLPGVDPSGNSPKLSIN-----------GSGSRSI 162
Query: 462 VRPKKRGRPRGSKNKVNNIMEVSKKAASG---GDCKIAGPKKCGRPKGSKNKQKNIVQVS 518
G G + + + + + A G +I ++ GRP GSKNK K + V+
Sbjct: 163 -----GGDGEGGNSNIRDDEDEERDAGDEPREGAVEIGNRRQRGRPPGSKNKPKPPIFVT 327
Query: 519 QEVAGSAD---CEIAGPKKCGRPKGSMKKRKSL-VCASILEGAGGITREGLENKMLSNLC 574
++ S E+AG +R+ VC +L G+G + L
Sbjct: 328 RDSPNSLRSHVMEVAGGADVAESVAQFARRRQRGVC--VLSGSGAVANVTLRQPAAPGAV 501
Query: 575 Q------EHIEYTQPVVRGGRPKGSRNKKIKLA 601
E + T + G P GS + LA
Sbjct: 502 VALHGRFEILSLTGAFLPGPAPPGSTGLTVYLA 600
>BP038715
Length = 530
Score = 30.0 bits (66), Expect = 2.9
Identities = 13/24 (54%), Positives = 16/24 (66%)
Frame = +2
Query: 666 QNDKSSVKPKRGRPKGSKNKMKSI 689
Q + S +RGRPKGSKNK K +
Sbjct: 143 QTNSSQKSAQRGRPKGSKNKPKPL 214
>TC11910 similar to GB|CAA54498.1|602891|SCIILDNA YBL0422 {Saccharomyces
cerevisiae;} , partial (6%)
Length = 638
Score = 30.0 bits (66), Expect = 2.9
Identities = 20/47 (42%), Positives = 25/47 (52%), Gaps = 1/47 (2%)
Frame = -1
Query: 111 LGCESLNLQDK-GEEGQQVHSGGFGEGCGRMGQVLGDYGVEYAEDRN 156
LG L+L+ K G EG+ GG GEGCGR G+ E ED +
Sbjct: 197 LGGLCLHLEGKRGREGKWDLDGGGGEGCGRE*DEHGER*SEVEEDED 57
>TC10673 homologue to UP|Q8S8Z0 (Q8S8Z0) Protein phosphatase 2C, partial
(48%)
Length = 669
Score = 28.5 bits (62), Expect = 8.5
Identities = 15/47 (31%), Positives = 20/47 (41%)
Frame = +2
Query: 778 YGLRSSGLMGDTERKKETRILRCHQCWRNSWSGVVICAKCKRKQYCY 824
YGL S + ++++ CH CW C KC R Q CY
Sbjct: 536 YGL*ISEVRK*SKQRCWINCFHCHSCW-----XPFACCKCWRLQSCY 661
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.315 0.135 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 27,709,195
Number of Sequences: 28460
Number of extensions: 411958
Number of successful extensions: 1672
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 1617
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1668
length of query: 1541
length of database: 4,897,600
effective HSP length: 102
effective length of query: 1439
effective length of database: 1,994,680
effective search space: 2870344520
effective search space used: 2870344520
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0103.12