BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0101.14
(1733 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC13225 124 1e-28
BG662087 66 5e-11
AV410603 45 1e-04
TC18698 37 0.020
TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 36 0.045
TC16847 weakly similar to PIR|T51733|T51733 cytidine deaminase ... 35 0.10
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 35 0.10
AV420911 35 0.10
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 35 0.13
TC17162 similar to UP|Q8H5F1 (Q8H5F1) OJ1165_F02.16 protein, par... 34 0.17
TC10910 similar to UP|Q9LHN2 (Q9LHN2) Gb|AAC26693.1, partial (32%) 34 0.22
BP036801 34 0.22
TC8136 similar to GB|AAB60729.1|2160166|F21M12 F21M12.13 {Arabid... 33 0.50
TC16374 similar to UP|Q7LZA6 (Q7LZA6) Protamine II (Fragment), p... 33 0.50
AV421607 33 0.50
TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 32 0.65
BI418821 32 0.85
BP039346 32 1.1
BP053311 30 3.2
BP041751 29 5.5
>TC13225
Length = 513
Score = 124 bits (312), Expect = 1e-28
Identities = 63/162 (38%), Positives = 95/162 (57%), Gaps = 2/162 (1%)
Frame = +3
Query: 680 VKTDEEGNPIIEDGKLIS--DAVNSLIFTIAQHFVGDPSLIKDRSGDLLSNLKCKSLGDF 737
+ T E+ + I+E K DAV +LI+TI HF+GDPS+ ++R+ L+NL C ++ D+
Sbjct: 3 IMTPEDKSTILEKKKENGEEDAVATLIYTIILHFIGDPSIFRERASSQLANLYCPTMSDY 182
Query: 738 RWYKDTFLTRVYTREDSQQAFWKEKFLAGLPKSFGDKVREKLRSQNPGGEIPYQTLSYGQ 797
RWYKDTF ++V RED AFWKE+F+AGLP+ KV + L N G + + +LS+GQ
Sbjct: 183 RWYKDTFFSKVTLREDGNSAFWKERFIAGLPRLMQSKVLDNLSLFNQGNPVNFGSLSFGQ 362
Query: 798 LIAIIQRVALKICQDDKIQQQLTKEKSQNRRDLGTFCEQFGI 839
L I ++ + +SQ R +FCE +G+
Sbjct: 363 LHNTIVHTGIQFVLTSNFKTNAKDMQSQKRS--SSFCEXYGV 482
>BG662087
Length = 373
Score = 65.9 bits (159), Expect = 5e-11
Identities = 37/113 (32%), Positives = 62/113 (54%), Gaps = 1/113 (0%)
Frame = +1
Query: 1362 GTPRLVINYKPLNQALCWIRYPIPNKKDLLARLHDAKIFSKFDMKSGFWQIQLQEKDRYK 1421
G R+ ++Y LN+A YP+P+ L+ D ++ S D SG+ QI++ D K
Sbjct: 16 GKWRMWVDYTDLNKACPKDSYPLPSIDKLVDGASDNELLSLMDAYSGYHQIKMHPSDEDK 195
Query: 1422 TAFTVPFGQYEWNVMPFGLKNAPSEFQRIMNEIFNPYSNFTI-VYIDDVLIFS 1473
TAF Y + +PFGLKNA + +Q +M+ +F + VY+D++++ S
Sbjct: 196 TAFMTARVNYCYQTIPFGLKNAGATYQXLMDRVFXDXVGRNMEVYLDNMIVKS 354
>AV410603
Length = 162
Score = 44.7 bits (104), Expect = 1e-04
Identities = 19/48 (39%), Positives = 31/48 (64%)
Frame = +1
Query: 1382 YPIPNKKDLLARLHDAKIFSKFDMKSGFWQIQLQEKDRYKTAFTVPFG 1429
+P+P +LL L ++ FSK D++SG+ QI ++ +DR+KT F G
Sbjct: 19 FPMPTVDELLDELRGSQFFSKLDLRSGYHQILVKPEDRHKTVFRTHHG 162
>TC18698
Length = 808
Score = 37.4 bits (85), Expect = 0.020
Identities = 19/54 (35%), Positives = 33/54 (60%), Gaps = 1/54 (1%)
Frame = -2
Query: 1421 KTAFTVPFGQYEWNVMPFGLKNAPSEFQRIMNEIFNPYSNFTI-VYIDDVLIFS 1473
KT + Y + VMP GLKN + +QR+M++IF+ + VY++D+++ S
Sbjct: 801 KTTLKINRVNYYYQVMPLGLKNI*TTYQRLMDKIFHKQI*KNVEVYVEDMIVKS 640
>TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (89%)
Length = 912
Score = 36.2 bits (82), Expect = 0.045
Identities = 11/22 (50%), Positives = 17/22 (77%)
Frame = +1
Query: 895 GKNVTCYNCGKPGHISRYCRLK 916
G ++ CY CG+PGH +R CR++
Sbjct: 364 GSDMKCYECGEPGHFARECRMR 429
>TC16847 weakly similar to PIR|T51733|T51733 cytidine deaminase [validated]
- Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (74%)
Length = 1098
Score = 35.0 bits (79), Expect = 0.10
Identities = 20/59 (33%), Positives = 27/59 (44%), Gaps = 4/59 (6%)
Frame = +2
Query: 845 KPKPRKHDPPPKQQWRRNSSRNHDHRK----PKPRSKPHSTQAAKNPPENRPSQGKNVT 899
KP+P H P R R H HR+ P+P PH + +P + RP G +VT
Sbjct: 143 KPRPCPHLQIPGSSGGRRRLRPHLHRRQPRVPRPPLPPHRPRRTVSPNQPRPQCGDSVT 319
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 35.0 bits (79), Expect = 0.10
Identities = 11/20 (55%), Positives = 16/20 (80%)
Frame = +1
Query: 895 GKNVTCYNCGKPGHISRYCR 914
G+++ CY CG+PGH +R CR
Sbjct: 151 GEDLKCYECGEPGHFARECR 210
>AV420911
Length = 418
Score = 35.0 bits (79), Expect = 0.10
Identities = 11/20 (55%), Positives = 16/20 (80%)
Frame = +2
Query: 895 GKNVTCYNCGKPGHISRYCR 914
G+++ CY CG+PGH +R CR
Sbjct: 359 GEDLKCYECGEPGHFARECR 418
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 34.7 bits (78), Expect = 0.13
Identities = 17/53 (32%), Positives = 23/53 (43%)
Frame = +2
Query: 861 RNSSRNHDHRKPKPRSKPHSTQAAKNPPENRPSQGKNVTCYNCGKPGHISRYC 913
R R+ D R + RS+ + N E C+ CGKPGH +R C
Sbjct: 332 RERGRDRDDRGDRDRSRGYGGSRGSNGGE----------CFKCGKPGHFAREC 460
>TC17162 similar to UP|Q8H5F1 (Q8H5F1) OJ1165_F02.16 protein, partial (40%)
Length = 784
Score = 34.3 bits (77), Expect = 0.17
Identities = 21/53 (39%), Positives = 27/53 (50%), Gaps = 4/53 (7%)
Frame = +2
Query: 846 PKPRKHDPPPKQQWRRNSSRNHDHRKPKPRSKPHSTQAAK----NPPENRPSQ 894
P PR+ PPP Q RR + R HR+P+PR A + +PPE P Q
Sbjct: 233 PPPRQR-PPPHPQTRRANPR---HRRPRPRRHRQRQDALEPRDSHPPEEAPHQ 379
>TC10910 similar to UP|Q9LHN2 (Q9LHN2) Gb|AAC26693.1, partial (32%)
Length = 595
Score = 33.9 bits (76), Expect = 0.22
Identities = 18/52 (34%), Positives = 21/52 (39%), Gaps = 3/52 (5%)
Frame = -1
Query: 846 PKPRKHDPPPKQQWRRNSSRNHDHRKP---KPRSKPHSTQAAKNPPENRPSQ 894
P P PP Q W N S R P + R P T + PPE PS+
Sbjct: 160 PNPSPSPPPETQTWATNCSPRRTRRSPATSRRR*PPPHTTTPRPPPETSPSR 5
>BP036801
Length = 540
Score = 33.9 bits (76), Expect = 0.22
Identities = 17/47 (36%), Positives = 27/47 (57%)
Frame = +3
Query: 844 KKPKPRKHDPPPKQQWRRNSSRNHDHRKPKPRSKPHSTQAAKNPPEN 890
++P PR+H PP ++ RR H H P P ++P + A+ PPE+
Sbjct: 345 RRPHPRRHPPPNRRLQRRLPQAPHLH--PLPTTQP--LRLARTPPES 473
>TC8136 similar to GB|AAB60729.1|2160166|F21M12 F21M12.13 {Arabidopsis
thaliana;}, partial (70%)
Length = 1659
Score = 32.7 bits (73), Expect = 0.50
Identities = 17/27 (62%), Positives = 18/27 (65%), Gaps = 2/27 (7%)
Frame = -3
Query: 513 SCSPVAVSAPAPPTAA--PEPVIRRSS 537
SCSP+A S PAPP AA P RRSS
Sbjct: 1219 SCSPMAASGPAPPNAA*LQVPASRRSS 1139
>TC16374 similar to UP|Q7LZA6 (Q7LZA6) Protamine II (Fragment), partial (63%)
Length = 1130
Score = 32.7 bits (73), Expect = 0.50
Identities = 49/201 (24%), Positives = 83/201 (40%), Gaps = 25/201 (12%)
Frame = +3
Query: 914 RLKRRISELHLEPEIEDKINNLLIQTSDEEESASSDSEVSEDLNQIQNDDDPQSSSSINV 973
RL+RR L L +E K+ D + +SD+ SE+ + QN+DD S+S
Sbjct: 66 RLRRRTCLLRLLASLERKMIKRRFYRLDRGDKDASDASSSEEDDYEQNNDDDAGSTSSGY 245
Query: 974 LTNEQDLLFRAINSI----PDPD-----EKKIYLER------------LKFTLEDKPPKN 1012
+ + + +NS D D EK++ + R E+KP
Sbjct: 246 ESEDSSVKEVDVNSAGLLSSDDDAGTIREKQVLINRELPNKRYSEKSNASAMAEEKPLPT 425
Query: 1013 PITTNKFNLRDTFR-RLEKSTI--KPVTIQD-LQSEVHTLQAEVKSLKQIQISQQLILDK 1068
+T+ + F+ R+ I T++D LQS+ H K KQ ++ L ++
Sbjct: 426 EMTSYILQCKSVFKCRICPRIICLSEDTLRDHLQSKRHA--RSEKLYKQGRLKAMLNING 599
Query: 1069 LTEENSEESSSSSSTPNSASN 1089
EN E+S + T ++ N
Sbjct: 600 -EIENDEDSETEIQTEDTEEN 659
>AV421607
Length = 245
Score = 32.7 bits (73), Expect = 0.50
Identities = 18/53 (33%), Positives = 22/53 (40%)
Frame = +3
Query: 888 PENRPSQGKNVTCYNCGKPGHISRYCRLKRRISELHLEPEIEDKINNLLIQTS 940
P + G CY CG+PGH SR C S + P N LL +S
Sbjct: 18 PGSGSGSGTATGCYKCGRPGHWSRDCPSSAPNSNPNPNPNTTTTPNPLLPSSS 176
>TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (26%)
Length = 555
Score = 32.3 bits (72), Expect = 0.65
Identities = 17/53 (32%), Positives = 26/53 (48%)
Frame = +2
Query: 861 RNSSRNHDHRKPKPRSKPHSTQAAKNPPENRPSQGKNVTCYNCGKPGHISRYC 913
R+ SR+ K RS S + A ++R ++ C NC +PGH +R C
Sbjct: 227 RSRSRSRSPMDRKIRSDRFSYRDAPYRRDSRRGFSRDNLCKNCKRPGHFAREC 385
Score = 30.4 bits (67), Expect = 2.5
Identities = 18/58 (31%), Positives = 26/58 (44%), Gaps = 2/58 (3%)
Frame = +2
Query: 861 RNSSRNHDHRKPKPR--SKPHSTQAAKNPPENRPSQGKNVTCYNCGKPGHISRYCRLK 916
R S R+ +R+ R S+ + + K P C+NCG PGHI+ C K
Sbjct: 278 RFSYRDAPYRRDSRRGFSRDNLCKNCKRPGHFARECPNVAICHNCGLPGHIASECTTK 451
>BI418821
Length = 614
Score = 32.0 bits (71), Expect = 0.85
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +2
Query: 890 NRPSQGKNVTCYNCGKPGHISRYC 913
N S G CYNCG GH++R C
Sbjct: 527 NNNSGGGGAGCYNCGDTGHLARDC 598
Score = 31.2 bits (69), Expect = 1.5
Identities = 11/24 (45%), Positives = 13/24 (53%)
Frame = +2
Query: 890 NRPSQGKNVTCYNCGKPGHISRYC 913
N G CYNCG GH++R C
Sbjct: 446 NNGGGGGGAACYNCGDAGHLARDC 517
Score = 28.9 bits (63), Expect = 7.2
Identities = 9/14 (64%), Positives = 11/14 (78%)
Frame = +2
Query: 900 CYNCGKPGHISRYC 913
CYNCG GH++R C
Sbjct: 392 CYNCGDTGHLARDC 433
>BP039346
Length = 470
Score = 31.6 bits (70), Expect = 1.1
Identities = 23/78 (29%), Positives = 27/78 (34%), Gaps = 14/78 (17%)
Frame = -1
Query: 843 PKKPKPRKHDPPPKQQWRRNSSRNHD--HRKPKPRSKPHST------------QAAKNPP 888
P PK R H P P +SS + + P P PHS Q PP
Sbjct: 437 PNPPKHRLHHPQPSSSSSSSSSSSSSAAPKHPLPLPHPHSAPCC*LGLACSP*QLPPEPP 258
Query: 889 ENRPSQGKNVTCYNCGKP 906
R S V CG+P
Sbjct: 257 SRRVSSLCLVQALRCGEP 204
>BP053311
Length = 529
Score = 30.0 bits (66), Expect = 3.2
Identities = 16/72 (22%), Positives = 25/72 (34%), Gaps = 10/72 (13%)
Frame = +2
Query: 827 RRDLGTFCEQFGIQGCP----------KKPKPRKHDPPPKQQWRRNSSRNHDHRKPKPRS 876
+ D+ +F Q G+ +K KP P+ + + N H P P S
Sbjct: 29 KSDIASFASQLGLSTSQSYSGFNDVDFRKTKPNTEKATPQNTQKPKNDTNRPHEHPNPNS 208
Query: 877 KPHSTQAAKNPP 888
KP + P
Sbjct: 209 KPKXKXRPRXXP 244
>BP041751
Length = 471
Score = 29.3 bits (64), Expect = 5.5
Identities = 25/88 (28%), Positives = 35/88 (39%), Gaps = 9/88 (10%)
Frame = +2
Query: 816 QQQLTKEKSQNRRDLGTFCEQFGIQGCPKKPKPRKHDPPPKQQWRRNSSRNHDHRKPKPR 875
Q Q+ K K+ G F F + + R+H P + Q RR R P R
Sbjct: 83 QNQIPKAKTSFIFPYGVFYSCFSHRXW----RXRRHGPKXRPQHRR-------QRLPNLR 229
Query: 876 SKPHSTQAAKN---------PPENRPSQ 894
+PH Q+ +N PP +RP Q
Sbjct: 230 LQPHHLQSRRNRRSGPPRRLPPSHRPVQ 313
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.339 0.149 0.490
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 31,792,434
Number of Sequences: 28460
Number of extensions: 492054
Number of successful extensions: 5245
Number of sequences better than 10.0: 86
Number of HSP's better than 10.0 without gapping: 4585
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5097
length of query: 1733
length of database: 4,897,600
effective HSP length: 103
effective length of query: 1630
effective length of database: 1,966,220
effective search space: 3204938600
effective search space used: 3204938600
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.8 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0101.14