BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0098e.6
(402 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC7878 homologue to UP|Q9M1K4 (Q9M1K4) Leucine zipper-containing... 817 0.0
AV414511 272 8e-74
AV765237 100 7e-22
AU251434 30 0.72
CB828049 29 1.2
BF177610 28 2.1
TC13171 28 2.1
TC14103 similar to UP|Q84XC6 (Q84XC6) Plasma intrinsic protein 2... 28 2.1
TC8595 similar to PIR|T09390|T09390 21K protein precursor - alfa... 28 2.1
BP039439 28 2.7
AV773330 28 2.7
AV428128 28 2.7
TC18933 similar to GB|AAL90935.1|19699138|AY090274 AT3g18790/MVE... 28 3.6
TC18462 weakly similar to UP|Q945M3 (Q945M3) At1g23890/T23E23_13... 28 3.6
TC14637 weakly similar to UP|FUS_HUMAN (P35637) RNA-binding prot... 27 6.1
TC14463 similar to UP|Q9SLF1 (Q9SLF1) Nodulin-like protein (At2g... 27 6.1
TC12466 similar to UP|Q8RX71 (Q8RX71) At3g51780/ORF3, partial (18%) 27 6.1
TC8094 homologue to UP|EFTU_PEA (O24310) Elongation factor Tu, c... 27 6.1
TC14842 similar to UP|RK4_TOBAC (O80361) 50S ribosomal protein L... 23 6.4
BP057687 27 7.9
>TC7878 homologue to UP|Q9M1K4 (Q9M1K4) Leucine zipper-containing protein
AT103, partial (90%)
Length = 1496
Score = 817 bits (2110), Expect = 0.0
Identities = 402/402 (100%), Positives = 402/402 (100%)
Frame = +3
Query: 1 MALVKPISKFTNAAPKFSNPRTISHSKFSTVRMSATTTPPSSTKPSKKANKTSIKETLLT 60
MALVKPISKFTNAAPKFSNPRTISHSKFSTVRMSATTTPPSSTKPSKKANKTSIKETLLT
Sbjct: 36 MALVKPISKFTNAAPKFSNPRTISHSKFSTVRMSATTTPPSSTKPSKKANKTSIKETLLT 215
Query: 61 PRFYTTDFDEMETLFNTEINKNLNEHEFEALLQEFKTDYNQTHFVRNKEFKEAADKLDGP 120
PRFYTTDFDEMETLFNTEINKNLNEHEFEALLQEFKTDYNQTHFVRNKEFKEAADKLDGP
Sbjct: 216 PRFYTTDFDEMETLFNTEINKNLNEHEFEALLQEFKTDYNQTHFVRNKEFKEAADKLDGP 395
Query: 121 LRQIFVEFLERSCTAEFSGFLLYKELGRRLKKTNPVVAEIFSLMSRDEARHAGFLNKGLS 180
LRQIFVEFLERSCTAEFSGFLLYKELGRRLKKTNPVVAEIFSLMSRDEARHAGFLNKGLS
Sbjct: 396 LRQIFVEFLERSCTAEFSGFLLYKELGRRLKKTNPVVAEIFSLMSRDEARHAGFLNKGLS 575
Query: 181 DFNLALDLGFLTKARKYTFFKPKFIFYATYLSEKIGYWRYITIYRHLKANPEYQCYPIFK 240
DFNLALDLGFLTKARKYTFFKPKFIFYATYLSEKIGYWRYITIYRHLKANPEYQCYPIFK
Sbjct: 576 DFNLALDLGFLTKARKYTFFKPKFIFYATYLSEKIGYWRYITIYRHLKANPEYQCYPIFK 755
Query: 241 YFENWCQDENRHGDFFSALMKAQPQFLNDWKAKLWSRFFCLSVYVTMYLNDCQRTDFYEG 300
YFENWCQDENRHGDFFSALMKAQPQFLNDWKAKLWSRFFCLSVYVTMYLNDCQRTDFYEG
Sbjct: 756 YFENWCQDENRHGDFFSALMKAQPQFLNDWKAKLWSRFFCLSVYVTMYLNDCQRTDFYEG 935
Query: 301 IGLNTKEFDMHVIIETNRTTARIFPAVLDVENPEFKRRLDRMVEINEKILAVGESDDIPF 360
IGLNTKEFDMHVIIETNRTTARIFPAVLDVENPEFKRRLDRMVEINEKILAVGESDDIPF
Sbjct: 936 IGLNTKEFDMHVIIETNRTTARIFPAVLDVENPEFKRRLDRMVEINEKILAVGESDDIPF 1115
Query: 361 VKNLKRIPLIAALASELLATYLMPPIESGSVDLAEFEPQLVY 402
VKNLKRIPLIAALASELLATYLMPPIESGSVDLAEFEPQLVY
Sbjct: 1116VKNLKRIPLIAALASELLATYLMPPIESGSVDLAEFEPQLVY 1241
>AV414511
Length = 424
Score = 272 bits (695), Expect = 8e-74
Identities = 141/157 (89%), Positives = 141/157 (89%)
Frame = +2
Query: 20 PRTISHSKFSTVRMSATTTPPSSTKPSKKANKTSIKETLLTPRFYTTDFDEMETLFNTEI 79
PRTISHSKFSTVRMSATTTPPSSTKPSKKANKTSIKETL FNTEI
Sbjct: 2 PRTISHSKFSTVRMSATTTPPSSTKPSKKANKTSIKETL----------------FNTEI 133
Query: 80 NKNLNEHEFEALLQEFKTDYNQTHFVRNKEFKEAADKLDGPLRQIFVEFLERSCTAEFSG 139
NKNLNEHEFEALLQEFKTDYNQTHFVRNKEFKEAADKLDGPLRQIFVEFLERSCTAEFSG
Sbjct: 134 NKNLNEHEFEALLQEFKTDYNQTHFVRNKEFKEAADKLDGPLRQIFVEFLERSCTAEFSG 313
Query: 140 FLLYKELGRRLKKTNPVVAEIFSLMSRDEARHAGFLN 176
FLLYKELGRRLKKTNPVVAEIFSLMSRDEARHAGFLN
Sbjct: 314 FLLYKELGRRLKKTNPVVAEIFSLMSRDEARHAGFLN 424
>AV765237
Length = 324
Score = 99.8 bits (247), Expect = 7e-22
Identities = 52/61 (85%), Positives = 55/61 (89%)
Frame = -2
Query: 342 MVEINEKILAVGESDDIPFVKNLKRIPLIAALASELLATYLMPPIESGSVDLAEFEPQLV 401
MVEI KILAVGES+DIPFVKNLKRIPLIAALAS+L ATYLMPP ESGSV LAEF+PQ V
Sbjct: 323 MVEITTKILAVGESEDIPFVKNLKRIPLIAALASKLWATYLMPPAESGSVYLAEFKPQHV 144
Query: 402 Y 402
Y
Sbjct: 143 Y 141
>AU251434
Length = 371
Score = 30.0 bits (66), Expect = 0.72
Identities = 18/75 (24%), Positives = 38/75 (50%), Gaps = 4/75 (5%)
Frame = +1
Query: 63 FYTTDFDEM-ETLFNTEINKNLNEHEFEALLQEFK---TDYNQTHFVRNKEFKEAADKLD 118
F T F++ L N ++ ++ N+H F+ +E+K D++ FV N++ + +K
Sbjct: 91 FNTNSFEQFCINLTNEKLQQHFNQHVFKMEQEEYKKEEIDWSYIEFVDNQDILDLIEKKP 270
Query: 119 GPLRQIFVEFLERSC 133
G + + L+ +C
Sbjct: 271 GGI----IALLDEAC 303
>CB828049
Length = 529
Score = 29.3 bits (64), Expect = 1.2
Identities = 12/26 (46%), Positives = 13/26 (49%)
Frame = +3
Query: 230 NPEYQCYPIFKYFENWCQDENRHGDF 255
NP Q P+ F WC NRHG F
Sbjct: 450 NPIDQQIPVSLQFGXWCVQRNRHGKF 527
>BF177610
Length = 257
Score = 28.5 bits (62), Expect = 2.1
Identities = 19/52 (36%), Positives = 24/52 (45%)
Frame = +1
Query: 39 PPSSTKPSKKANKTSIKETLLTPRFYTTDFDEMETLFNTEINKNLNEHEFEA 90
P S+T+ S K SI LLTP F+ ETL N +N N +A
Sbjct: 40 PLSATQRSLFKRKPSIPSPLLTPNL----FNPSETLHNKPLNTEFNWKRLKA 183
>TC13171
Length = 598
Score = 28.5 bits (62), Expect = 2.1
Identities = 15/41 (36%), Positives = 21/41 (50%)
Frame = +1
Query: 6 PISKFTNAAPKFSNPRTISHSKFSTVRMSATTTPPSSTKPS 46
P+++ P FS SH F+ ++ T PPSST PS
Sbjct: 325 PLARPHRTLPWFSTQGVNSHG-FTAKKLQTQTPPPSSTHPS 444
>TC14103 similar to UP|Q84XC6 (Q84XC6) Plasma intrinsic protein 2,2, partial
(94%)
Length = 1199
Score = 28.5 bits (62), Expect = 2.1
Identities = 16/35 (45%), Positives = 22/35 (62%), Gaps = 2/35 (5%)
Frame = +1
Query: 29 STVRMSATTTPP--SSTKPSKKANKTSIKETLLTP 61
ST S +++PP SST PS ++ T+ K T LTP
Sbjct: 199 STEPSSPSSSPPCSSSTSPSSPSSATAAKPTPLTP 303
>TC8595 similar to PIR|T09390|T09390 21K protein precursor - alfalfa
{Medicago sativa;}, partial (93%)
Length = 811
Score = 28.5 bits (62), Expect = 2.1
Identities = 14/43 (32%), Positives = 22/43 (50%)
Frame = +2
Query: 11 TNAAPKFSNPRTISHSKFSTVRMSATTTPPSSTKPSKKANKTS 53
+N +P P +H+ S + + +T PS KPS + KTS
Sbjct: 158 SNPSPPTPPPSNKTHTSSSKLHSPSPSTEPSPPKPSSQDAKTS 286
>BP039439
Length = 564
Score = 28.1 bits (61), Expect = 2.7
Identities = 24/85 (28%), Positives = 39/85 (45%), Gaps = 3/85 (3%)
Frame = +1
Query: 15 PKFSNPRTISHSKFSTVRMSATTTPPSSTKPSKKANK---TSIKETLLTPRFYTTDFDEM 71
P FS+P ++ S ++V +S +TP S+KPS + + L RF DF
Sbjct: 115 PSFSSPMKLAGSFPTSVALSTMSTP--SSKPSSARSDQIGSQFPSRLFLDRFDLLDF--R 282
Query: 72 ETLFNTEINKNLNEHEFEALLQEFK 96
+FNT+ + F+ L F+
Sbjct: 283 AKMFNTDYS-----FRFQILFYHFR 342
>AV773330
Length = 236
Score = 28.1 bits (61), Expect = 2.7
Identities = 11/23 (47%), Positives = 16/23 (68%)
Frame = +1
Query: 188 LGFLTKARKYTFFKPKFIFYATY 210
+GF T RK T+ +P+FI + TY
Sbjct: 40 MGFTTLIRKKTYREPRFIVFITY 108
>AV428128
Length = 415
Score = 28.1 bits (61), Expect = 2.7
Identities = 20/46 (43%), Positives = 26/46 (56%), Gaps = 2/46 (4%)
Frame = +1
Query: 3 LVKPISKFTNAAP-KFSNP-RTISHSKFSTVRMSATTTPPSSTKPS 46
L P ++T P NP RT+S + ST SATT+PPS T P+
Sbjct: 55 LPSPTDQWTRILPLPDPNPKRTLSSATRST--SSATTSPPSKTSPA 186
>TC18933 similar to GB|AAL90935.1|19699138|AY090274 AT3g18790/MVE11_17
{Arabidopsis thaliana;}, partial (63%)
Length = 609
Score = 27.7 bits (60), Expect = 3.6
Identities = 15/37 (40%), Positives = 22/37 (58%), Gaps = 3/37 (8%)
Frame = +3
Query: 32 RMSATTTPPS---STKPSKKANKTSIKETLLTPRFYT 65
R +A ++PPS S KP+ A+K+ K +PRF T
Sbjct: 105 RNAAPSSPPSAATSPKPTNGASKSCAKSAAKSPRFRT 215
>TC18462 weakly similar to UP|Q945M3 (Q945M3) At1g23890/T23E23_13, partial
(26%)
Length = 755
Score = 27.7 bits (60), Expect = 3.6
Identities = 17/46 (36%), Positives = 25/46 (53%), Gaps = 1/46 (2%)
Frame = +3
Query: 16 KFSNPRTISHSKFSTVRMSATTTP-PSSTKPSKKANKTSIKETLLT 60
K S P+T++ S + S T+TP PSS+ P + SI T L+
Sbjct: 126 KLSFPKTVTPSPPCSTATSITSTPIPSSSDPVPPISSFSIPSTALS 263
>TC14637 weakly similar to UP|FUS_HUMAN (P35637) RNA-binding protein FUS
(Oncogene FUS) (Oncogene TLS) (Translocated in
liposarcoma protein) (POMp75) (75 kDa DNA-pairing
protein), partial (6%)
Length = 1332
Score = 26.9 bits (58), Expect = 6.1
Identities = 20/61 (32%), Positives = 33/61 (53%), Gaps = 5/61 (8%)
Frame = +2
Query: 3 LVKPISKFTNAA--PKFSNPRTISHSK--FSTV-RMSATTTPPSSTKPSKKANKTSIKET 57
L + +S+F+ A P S P IS S + TV + S+ TPP S+KP+ + + +T
Sbjct: 29 LYQTVSQFSPALMPPVSSKPNVISGSSLPYQTVSQFSSALTPPVSSKPNAISGSGLLYQT 208
Query: 58 L 58
+
Sbjct: 209V 211
>TC14463 similar to UP|Q9SLF1 (Q9SLF1) Nodulin-like protein
(At2g16660/T24I21.7), partial (40%)
Length = 1122
Score = 26.9 bits (58), Expect = 6.1
Identities = 16/58 (27%), Positives = 30/58 (51%), Gaps = 2/58 (3%)
Frame = +2
Query: 6 PISKFTNAAPKFSNPR--TISHSKFSTVRMSATTTPPSSTKPSKKANKTSIKETLLTP 61
P + ++ P SNP T + S + + + T PSS+ + + KTS+K ++ +P
Sbjct: 140 PANGSASSPPSGSNPSPATTTPSPTTPTPSNPSCTSPSSSSTTSPSLKTSVKPSVSSP 313
>TC12466 similar to UP|Q8RX71 (Q8RX71) At3g51780/ORF3, partial (18%)
Length = 886
Score = 26.9 bits (58), Expect = 6.1
Identities = 17/62 (27%), Positives = 29/62 (46%), Gaps = 5/62 (8%)
Frame = -1
Query: 13 AAPKFSNPRTISHSKFS-----TVRMSATTTPPSSTKPSKKANKTSIKETLLTPRFYTTD 67
A+P S P ++S+ S T +S T PPS+ + + S+ + LTP +
Sbjct: 454 ASPSASIPSSVSNCLISNSVDTTNSLSETLVPPSTATSNAETLSESMSTSALTPAIASVA 275
Query: 68 FD 69
F+
Sbjct: 274 FN 269
>TC8094 homologue to UP|EFTU_PEA (O24310) Elongation factor Tu, chloroplast
precursor (EF-Tu), partial (60%)
Length = 1092
Score = 26.9 bits (58), Expect = 6.1
Identities = 19/59 (32%), Positives = 28/59 (47%), Gaps = 3/59 (5%)
Frame = +2
Query: 6 PISKFTNAAPKFSNPRTISHSKF---STVRMSATTTPPSSTKPSKKANKTSIKETLLTP 61
P S + + + PR+ + S S S +T PPSST+P + TS L+TP
Sbjct: 380 PPSPWRSPPSATAPPRSTTRSTLPLKSAPAASPSTPPPSSTRPRRATTHTS--TALVTP 550
>TC14842 similar to UP|RK4_TOBAC (O80361) 50S ribosomal protein L4,
chloroplast precursor (R-protein L4), partial (51%)
Length = 618
Score = 23.5 bits (49), Expect(2) = 6.4
Identities = 10/31 (32%), Positives = 17/31 (54%)
Frame = +1
Query: 15 PKFSNPRTISHSKFSTVRMSATTTPPSSTKP 45
P +P + S+FS + +PPS++KP
Sbjct: 181 PPSPSPASSQPSRFSPSPARKSASPPSTSKP 273
Score = 21.6 bits (44), Expect(2) = 6.4
Identities = 9/14 (64%), Positives = 10/14 (71%)
Frame = +1
Query: 40 PSSTKPSKKANKTS 53
PSST PS +KTS
Sbjct: 295 PSSTAPSSPTSKTS 336
>BP057687
Length = 573
Score = 26.6 bits (57), Expect = 7.9
Identities = 13/35 (37%), Positives = 17/35 (48%), Gaps = 4/35 (11%)
Frame = +3
Query: 244 NWCQDENRHGDFFS----ALMKAQPQFLNDWKAKL 274
NWC+++ H DF S A +P ND A L
Sbjct: 363 NWCEEKQAHYDFISPCKCANRPPKPSLFNDRLASL 467
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.137 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,445,890
Number of Sequences: 28460
Number of extensions: 88060
Number of successful extensions: 599
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 587
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 596
length of query: 402
length of database: 4,897,600
effective HSP length: 92
effective length of query: 310
effective length of database: 2,279,280
effective search space: 706576800
effective search space used: 706576800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0098e.6