BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0098b.4
(670 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9029 similar to UP|Q7X9G7 (Q7X9G7) Alpha-L-arabinofuranosidase... 1092 0.0
CN825301 369 e-103
TC15100 weakly similar to UP|BAC99303 (BAC99303) Alpha-L-arabino... 236 9e-63
TC9804 similar to UP|Q84RC5 (Q84RC5) Alpha-L-arabinofuranosidase... 191 4e-49
AU089495 106 6e-34
TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, ... 33 0.19
TC10703 similar to UP|BAD09184 (BAD09184) Eukaryotic translation... 30 0.96
TC9375 homologue to PIR|JA0049|JA0049 Tubulin beta-2 chain - soy... 29 2.8
TC12032 29 2.8
TC15345 28 4.8
TC8353 homologue to UP|HS7M_PHAVU (Q01899) Heat shock 70 kDa pro... 28 6.2
TC14452 similar to UP|Q94B60 (Q94B60) ATP-dependent Clp protease... 27 8.2
TC14318 similar to UP|O64739 (O64739) Ran-binding protein (AtRan... 27 8.2
>TC9029 similar to UP|Q7X9G7 (Q7X9G7) Alpha-L-arabinofuranosidase, partial
(73%)
Length = 1636
Score = 1092 bits (2824), Expect = 0.0
Identities = 530/530 (100%), Positives = 530/530 (100%)
Frame = +3
Query: 1 MGLYKASFIVAALQLCIAVSLCAIKVNADLNATLTVDAHETSGKPISETLFGLFFEEINH 60
MGLYKASFIVAALQLCIAVSLCAIKVNADLNATLTVDAHETSGKPISETLFGLFFEEINH
Sbjct: 45 MGLYKASFIVAALQLCIAVSLCAIKVNADLNATLTVDAHETSGKPISETLFGLFFEEINH 224
Query: 61 AGAGGLWAELVSNRGFEAGGPNIPSNIDPWSIIGNESFINVETDRTSCFDRNKIALRLEV 120
AGAGGLWAELVSNRGFEAGGPNIPSNIDPWSIIGNESFINVETDRTSCFDRNKIALRLEV
Sbjct: 225 AGAGGLWAELVSNRGFEAGGPNIPSNIDPWSIIGNESFINVETDRTSCFDRNKIALRLEV 404
Query: 121 LCDSKGDNICPADGVGVFNPGFWGMNIEQGKKYKVVFYVRSTGSLDLTVSLTGSNGVLAS 180
LCDSKGDNICPADGVGVFNPGFWGMNIEQGKKYKVVFYVRSTGSLDLTVSLTGSNGVLAS
Sbjct: 405 LCDSKGDNICPADGVGVFNPGFWGMNIEQGKKYKVVFYVRSTGSLDLTVSLTGSNGVLAS 584
Query: 181 NVITGSASDFSNWKKVETLLEAKATNHNSRLQLTTTAKGVIWLDQVSAMPLDTYKGRGFR 240
NVITGSASDFSNWKKVETLLEAKATNHNSRLQLTTTAKGVIWLDQVSAMPLDTYKGRGFR
Sbjct: 585 NVITGSASDFSNWKKVETLLEAKATNHNSRLQLTTTAKGVIWLDQVSAMPLDTYKGRGFR 764
Query: 241 SELVGMLADLKPRFIRFPGGCFVEGEWLRNAFRWKESIGPWEERPGHFGDVWKYWTDDGL 300
SELVGMLADLKPRFIRFPGGCFVEGEWLRNAFRWKESIGPWEERPGHFGDVWKYWTDDGL
Sbjct: 765 SELVGMLADLKPRFIRFPGGCFVEGEWLRNAFRWKESIGPWEERPGHFGDVWKYWTDDGL 944
Query: 301 GYFEFLQLAEDLGALPIWVFNNGVSHNDEVDTSAVLPFVQEALDGIEFARGDPTSKWGSI 360
GYFEFLQLAEDLGALPIWVFNNGVSHNDEVDTSAVLPFVQEALDGIEFARGDPTSKWGSI
Sbjct: 945 GYFEFLQLAEDLGALPIWVFNNGVSHNDEVDTSAVLPFVQEALDGIEFARGDPTSKWGSI 1124
Query: 361 RASMGHPEPFNLKYVAVGNEDCGKKNYRGNYLKFYSAIRSAYPDIQIISNCDGSSRPLDH 420
RASMGHPEPFNLKYVAVGNEDCGKKNYRGNYLKFYSAIRSAYPDIQIISNCDGSSRPLDH
Sbjct: 1125RASMGHPEPFNLKYVAVGNEDCGKKNYRGNYLKFYSAIRSAYPDIQIISNCDGSSRPLDH 1304
Query: 421 PADIYDYHIYTNANDMFSRSSTFNTALRSGPKAFVSEYAVTGNDAGTGSLLAAIAEAGFL 480
PADIYDYHIYTNANDMFSRSSTFNTALRSGPKAFVSEYAVTGNDAGTGSLLAAIAEAGFL
Sbjct: 1305PADIYDYHIYTNANDMFSRSSTFNTALRSGPKAFVSEYAVTGNDAGTGSLLAAIAEAGFL 1484
Query: 481 LGLEKNSDIVKMVSYAPLFVNANDRRWNPDAIVFNSFQSYGTPSYWVQLF 530
LGLEKNSDIVKMVSYAPLFVNANDRRWNPDAIVFNSFQSYGTPSYWVQLF
Sbjct: 1485LGLEKNSDIVKMVSYAPLFVNANDRRWNPDAIVFNSFQSYGTPSYWVQLF 1634
>CN825301
Length = 663
Score = 369 bits (947), Expect = e-103
Identities = 164/217 (75%), Positives = 192/217 (87%)
Frame = +2
Query: 193 WKKVETLLEAKATNHNSRLQLTTTAKGVIWLDQVSAMPLDTYKGRGFRSELVGMLADLKP 252
W+K+ET+LEAK TNH S LQ+TT+ GV+WLDQVSAMPLDTYKG GFR +L M+ADL P
Sbjct: 11 WRKIETILEAKDTNHYSSLQITTSLSGVVWLDQVSAMPLDTYKGHGFRKDLFQMVADLNP 190
Query: 253 RFIRFPGGCFVEGEWLRNAFRWKESIGPWEERPGHFGDVWKYWTDDGLGYFEFLQLAEDL 312
RF RFPGGCFVEG++LRNAFRWK+++G WEERPGHFGD W YWTDDGLGYFE LQLAEDL
Sbjct: 191 RFFRFPGGCFVEGDYLRNAFRWKDTVGSWEERPGHFGDKWHYWTDDGLGYFEGLQLAEDL 370
Query: 313 GALPIWVFNNGVSHNDEVDTSAVLPFVQEALDGIEFARGDPTSKWGSIRASMGHPEPFNL 372
GALP+WVFNNG+SHN+EV+T+ +LPFV EAL+GIEFARG PTS WGS RA+MGHP+PF+L
Sbjct: 371 GALPVWVFNNGISHNEEVNTTEILPFVLEALEGIEFARGSPTSYWGSRRAAMGHPKPFDL 550
Query: 373 KYVAVGNEDCGKKNYRGNYLKFYSAIRSAYPDIQIIS 409
+ VAVGNEDC NY+GNY+KFY+AI+ AYPDIQIIS
Sbjct: 551 RIVAVGNEDCSHYNYQGNYMKFYNAIKRAYPDIQIIS 661
>TC15100 weakly similar to UP|BAC99303 (BAC99303)
Alpha-L-arabinofuranosidase, partial (15%)
Length = 586
Score = 236 bits (602), Expect = 9e-63
Identities = 122/122 (100%), Positives = 122/122 (100%)
Frame = +1
Query: 549 SSSLIASAINWQSSADKKNYIRIKVVNFGANTVNLKISLNGLDPNSLQPSGSTRTVLTSA 608
SSSLIASAINWQSSADKKNYIRIKVVNFGANTVNLKISLNGLDPNSLQPSGSTRTVLTSA
Sbjct: 1 SSSLIASAINWQSSADKKNYIRIKVVNFGANTVNLKISLNGLDPNSLQPSGSTRTVLTSA 180
Query: 609 NVMDENSFSQPKKVVPIQSLLQNVGKEMNVVVPPHSFSSFDLLKESSNLKMVGGGSSTRS 668
NVMDENSFSQPKKVVPIQSLLQNVGKEMNVVVPPHSFSSFDLLKESSNLKMVGGGSSTRS
Sbjct: 181 NVMDENSFSQPKKVVPIQSLLQNVGKEMNVVVPPHSFSSFDLLKESSNLKMVGGGSSTRS 360
Query: 669 SI 670
SI
Sbjct: 361 SI 366
>TC9804 similar to UP|Q84RC5 (Q84RC5) Alpha-L-arabinofuranosidase, partial
(16%)
Length = 658
Score = 191 bits (484), Expect = 4e-49
Identities = 95/152 (62%), Positives = 122/152 (79%)
Frame = +3
Query: 501 NANDRRWNPDAIVFNSFQSYGTPSYWVQLFFSESSGATLLNSSLQTSSSSSLIASAINWQ 560
N+NDR W+PDAIVFNS Q YGTPSYW+Q F+ESSGATLL S+LQT SS SL+ASAI +
Sbjct: 3 NSNDRTWSPDAIVFNSHQHYGTPSYWLQRMFTESSGATLLKSTLQT-SSQSLVASAIEYA 179
Query: 561 SSADKKNYIRIKVVNFGANTVNLKISLNGLDPNSLQPSGSTRTVLTSANVMDENSFSQPK 620
+S DKKNYIR+KVVNFG+ + + +IS+NGL + ++ SGS +TVLT+ANVMDENSFS+P
Sbjct: 180 NSEDKKNYIRVKVVNFGSGSESFRISINGL-KSKVKQSGSRKTVLTAANVMDENSFSEPN 356
Query: 621 KVVPIQSLLQNVGKEMNVVVPPHSFSSFDLLK 652
K+VP + ++ +M V +PPHS +SFDLLK
Sbjct: 357 KIVPQPTPIEGASTDMKVELPPHSVTSFDLLK 452
>AU089495
Length = 453
Score = 106 bits (264), Expect(2) = 6e-34
Identities = 48/73 (65%), Positives = 58/73 (78%), Gaps = 1/73 (1%)
Frame = +2
Query: 384 KKNYRGNYLKFYSAIRSAYPDIQIISNCDGSSRPLDHPADIYDYHIY-TNANDMFSRSST 442
+KNY GNYL FY+AIR AYPDIQ+ISNCD SS+PLDHPAD+YDYH Y N ++MF+ +
Sbjct: 8 EKNYLGNYLAFYNAIRKAYPDIQMISNCDASSKPLDHPADLYDYHTYPNNPSNMFNNAHV 187
Query: 443 FNTALRSGPKAFV 455
F+ R GPKAFV
Sbjct: 188 FDKTPRKGPKAFV 226
Score = 55.5 bits (132), Expect(2) = 6e-34
Identities = 28/52 (53%), Positives = 38/52 (72%), Gaps = 1/52 (1%)
Frame = +3
Query: 452 KAFVSEYAVTGND-AGTGSLLAAIAEAGFLLGLEKNSDIVKMVSYAPLFVNA 502
+ +SEYA+ G+ A G+L+ ++EAGFL+GL KNSD V M +YAPLFV A
Sbjct: 216 RLLLSEYALVGDQQAKLGTLIGGVSEAGFLIGLXKNSDHVXMAAYAPLFVIA 371
>TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, partial
(31%)
Length = 727
Score = 32.7 bits (73), Expect = 0.19
Identities = 30/101 (29%), Positives = 49/101 (47%), Gaps = 5/101 (4%)
Frame = -3
Query: 494 SYAPLFVNANDRRWNPDA----IVFNSFQSYGTPSYWVQLFFSESSGATLLNSSLQTSSS 549
S A L + + RW A ++ SF G+ S+W+ S S+ A+ +SS +SSS
Sbjct: 452 SSATLRIRSATSRWLDAASARRLMICSFPM-GSRSFWIGSSSSSSASASTSSSSSSSSSS 276
Query: 550 SSLIASAINWQSSADKKNYIRIKVVNFG-ANTVNLKISLNG 589
SS +S+ + SS+ + ++ + NL SLNG
Sbjct: 275 SSPSSSSSSSSSSSSTSSSTSSSTSSYS*TSASNLFCSLNG 153
>TC10703 similar to UP|BAD09184 (BAD09184) Eukaryotic translation initiation
factor 3 subunit (EIF-3)-like, partial (16%)
Length = 1066
Score = 30.4 bits (67), Expect = 0.96
Identities = 25/76 (32%), Positives = 38/76 (49%), Gaps = 5/76 (6%)
Frame = +1
Query: 529 LFFSESSGATLLNSSLQTSSSSSLIASAINWQSSADKKNYIRI----KVVNFGANTVNLK 584
+FFS + A L S + S+S SA QSS+DK ++I + +V N G N +
Sbjct: 46 IFFSFAIDADLEKLSKKHPDSNSQTRSAGTLQSSSDKASHIVLHGDSQVPNGGKNDTSST 225
Query: 585 ISLNGLD-PNSLQPSG 599
LNG + + + P G
Sbjct: 226 EDLNGTEVTDDVSPEG 273
>TC9375 homologue to PIR|JA0049|JA0049 Tubulin beta-2 chain - soybean
{Glycine max;} , partial (15%)
Length = 699
Score = 28.9 bits (63), Expect = 2.8
Identities = 11/31 (35%), Positives = 18/31 (57%)
Frame = -2
Query: 419 DHPADIYDYHIYTNANDMFSRSSTFNTALRS 449
DH D+Y++ TN D+ SR+ ++T S
Sbjct: 449 DHDLDVYNHLTITNLQDLISRTEAWSTKFSS 357
>TC12032
Length = 486
Score = 28.9 bits (63), Expect = 2.8
Identities = 14/28 (50%), Positives = 17/28 (60%)
Frame = +1
Query: 89 PWSIIGNESFINVETDRTSCFDRNKIAL 116
PW I N SF+ + RTSCFD + I L
Sbjct: 355 PWKWIANFSFVGI---RTSCFDAHVIYL 429
>TC15345
Length = 635
Score = 28.1 bits (61), Expect = 4.8
Identities = 18/53 (33%), Positives = 27/53 (49%), Gaps = 3/53 (5%)
Frame = -3
Query: 592 PNSLQPSGSTRTVLTSANVM---DENSFSQPKKVVPIQSLLQNVGKEMNVVVP 641
PNS PSGS L +++V +NSF + +QSL+ V V++P
Sbjct: 429 PNSSNPSGSKNNPLNTSSVFTI*QKNSFHPILLTLQVQSLVLYVL*HSGVLIP 271
>TC8353 homologue to UP|HS7M_PHAVU (Q01899) Heat shock 70 kDa protein,
mitochondrial precursor, partial (74%)
Length = 1752
Score = 27.7 bits (60), Expect = 6.2
Identities = 23/71 (32%), Positives = 34/71 (47%), Gaps = 1/71 (1%)
Frame = +3
Query: 179 ASNVITGSASDFSNWKKVETLLEAKATNHNSRLQLTTTAKGVIWLDQVSAMPLDTYKGR- 237
A+ ++T SA D S K+ + + L ++ T K WL ++S M T KGR
Sbjct: 1050 ANGIVTVSAKDKSTGKEQQITIXG-------HLVVSQTMKSRRWLKKLSCMLRRTKKGRL 1208
Query: 238 GFRSELVGMLA 248
SE V +LA
Sbjct: 1209 SLISETVQILA 1241
>TC14452 similar to UP|Q94B60 (Q94B60) ATP-dependent Clp protease-like
protein (ATP-dependent Clp protease proteolytic
subunit) (Endopeptidase Clp) (At5g45390) , partial (36%)
Length = 621
Score = 27.3 bits (59), Expect = 8.2
Identities = 14/28 (50%), Positives = 19/28 (67%)
Frame = -1
Query: 164 SLDLTVSLTGSNGVLASNVITGSASDFS 191
S+DLT+S TGS G++ S IT S +S
Sbjct: 246 SVDLTLSGTGSRGIILSLSITPSMIPYS 163
>TC14318 similar to UP|O64739 (O64739) Ran-binding protein (AtRanBP1b),
partial (73%)
Length = 1099
Score = 27.3 bits (59), Expect = 8.2
Identities = 18/64 (28%), Positives = 35/64 (54%)
Frame = -3
Query: 499 FVNANDRRWNPDAIVFNSFQSYGTPSYWVQLFFSESSGATLLNSSLQTSSSSSLIASAIN 558
F++A+ ++PDA +S S FFS S+ ++ LN S++ +++S S++
Sbjct: 710 FLSASALLFSPDAGSCSS----ALLSVLTSSFFSASAFSSTLNFSIRPAAASDASLSSVF 543
Query: 559 WQSS 562
W S+
Sbjct: 542 WDSA 531
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.134 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,888,910
Number of Sequences: 28460
Number of extensions: 170435
Number of successful extensions: 802
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 790
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 798
length of query: 670
length of database: 4,897,600
effective HSP length: 96
effective length of query: 574
effective length of database: 2,165,440
effective search space: 1242962560
effective search space used: 1242962560
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0098b.4