BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0097b.12
(292 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC12338 similar to GB|AAP13422.1|30023778|BT006314 At2g01660 {Ar... 251 2e-86
TC12257 weakly similar to UP|Q9FHQ4 (Q9FHQ4) Similarity to secre... 169 5e-43
BP040765 89 1e-18
TC14519 75 2e-14
AW720134 72 1e-13
TC19031 similar to GB|AAR24233.1|38566686|BT010866 At1g04520 {Ar... 57 3e-09
AV767962 54 2e-08
AV766715 45 2e-05
BP081081 31 0.21
TC14463 similar to UP|Q9SLF1 (Q9SLF1) Nodulin-like protein (At2g... 28 1.4
TC19523 28 1.8
BP072459 27 3.1
BI417786 27 5.3
BP081173 27 5.3
TC10241 27 5.3
BP043877 27 5.3
TC12225 26 6.9
TC9025 similar to PIR|F86282|F86282 protein F10B6.30 [imported] ... 26 9.0
>TC12338 similar to GB|AAP13422.1|30023778|BT006314 At2g01660 {Arabidopsis
thaliana;}, partial (26%)
Length = 903
Score = 251 bits (641), Expect(2) = 2e-86
Identities = 128/154 (83%), Positives = 131/154 (84%), Gaps = 6/154 (3%)
Frame = -1
Query: 36 PKNTPVSDPVYETGVNSLLTSLVNSASFTNYNNFTVPATAGSGDTLYGLYQCRGDLNNDQ 95
P+ P+YETGVNSLLTSL NSASFTNYNNFTVPATAGSGDTLYGLYQCRGDLNNDQ
Sbjct: 729 PRTRRFPTPIYETGVNSLLTSLFNSASFTNYNNFTVPATAGSGDTLYGLYQCRGDLNNDQ 550
Query: 96 CYRCVARAVSQLGTLCSAVRGGALQLEGCFVKYDNVKFLGVEDKTMVVKKCGPSIGLTSD 155
YRCVARAVSQLGTLCSAVRGGALQLEGC VKYDNVK LGVEDKTMVVKKC PSIGLTSD
Sbjct: 549 RYRCVARAVSQLGTLCSAVRGGALQLEGCPVKYDNVKLLGVEDKTMVVKKCRPSIGLTSD 370
Query: 156 ALTRRDAVLAYLQTSDGVY------RTWRISAYG 183
A TRRDAVLAY+QTSDGVY R W S G
Sbjct: 369 ASTRRDAVLAYIQTSDGVYMDVEDQRLWGFSGCG 268
Score = 144 bits (362), Expect = 2e-35
Identities = 75/102 (73%), Positives = 80/102 (77%)
Frame = -2
Query: 176 TWRISAYGDFQGAAQCTGDLSPSECQDCLSESIQRLKTECGASTWGEMYLAKCYARYSEG 235
TWRISAYGDFQGAA CT DL+PSECQDCLSESIQRLKTECGASTW EMYLA CYA G
Sbjct: 308 TWRISAYGDFQGAAHCTVDLTPSECQDCLSESIQRLKTECGASTWREMYLANCYAALLRG 129
Query: 236 GDHSRHSDDDSNHNDDEIEKTLAILIGLIAGVALIIVFLSFL 277
+NHND+EI+KTLAI + LIA VALIIV LSFL
Sbjct: 128 LGSLPPQ*RYNNHNDNEIDKTLAI*L*LIAEVALIIVSLSFL 3
Score = 84.3 bits (207), Expect(2) = 2e-86
Identities = 43/44 (97%), Positives = 43/44 (97%)
Frame = -3
Query: 1 MVLLLRLLFILTLSAITTPSTSSSIDTFIFGGCSQPKNTPVSDP 44
MVLLLRLLFILTLSAITTPSTSSSIDTFIFGG SQPKNTPVSDP
Sbjct: 835 MVLLLRLLFILTLSAITTPSTSSSIDTFIFGGRSQPKNTPVSDP 704
Score = 34.3 bits (77), Expect = 0.025
Identities = 15/46 (32%), Positives = 24/46 (51%)
Frame = -1
Query: 187 GAAQCTGDLSPSECQDCLSESIQRLKTECGASTWGEMYLAKCYARY 232
G QC GDL+ + C++ ++ +L T C A G + L C +Y
Sbjct: 588 GLYQCRGDLNNDQRYRCVARAVSQLGTLCSAVRGGALQLEGCPVKY 451
>TC12257 weakly similar to UP|Q9FHQ4 (Q9FHQ4) Similarity to secretory
protein, partial (49%)
Length = 475
Score = 169 bits (428), Expect = 5e-43
Identities = 83/150 (55%), Positives = 109/150 (72%)
Frame = +2
Query: 2 VLLLRLLFILTLSAITTPSTSSSIDTFIFGGCSQPKNTPVSDPVYETGVNSLLTSLVNSA 61
+L+ L +L+LS SS +D F++GGC+Q + TP S YE +NSLLTSLVNSA
Sbjct: 53 LLIFANLIVLSLS-------SSQLDLFLYGGCTQQRYTPNSP--YELNLNSLLTSLVNSA 205
Query: 62 SFTNYNNFTVPATAGSGDTLYGLYQCRGDLNNDQCYRCVARAVSQLGTLCSAVRGGALQL 121
++++YNN+TV G+ D YGLYQCRGD++ C C++RAVS+ G LC RGGA+QL
Sbjct: 206 TYSSYNNYTV---VGADDVAYGLYQCRGDISMPDCATCISRAVSRAGVLCQEARGGAVQL 376
Query: 122 EGCFVKYDNVKFLGVEDKTMVVKKCGPSIG 151
EGC+VKYDN KFLG EDKT+V++KCGPS G
Sbjct: 377 EGCYVKYDNAKFLGAEDKTVVLRKCGPSAG 466
Score = 40.4 bits (93), Expect = 4e-04
Identities = 16/46 (34%), Positives = 24/46 (51%)
Frame = +2
Query: 187 GAAQCTGDLSPSECQDCLSESIQRLKTECGASTWGEMYLAKCYARY 232
G QC GD+S +C C+S ++ R C + G + L CY +Y
Sbjct: 260 GLYQCRGDISMPDCATCISRAVSRAGVLCQEARGGAVQLEGCYVKY 397
>BP040765
Length = 424
Score = 88.6 bits (218), Expect = 1e-18
Identities = 44/87 (50%), Positives = 55/87 (62%)
Frame = -2
Query: 163 VLAYLQTSDGVYRTWRISAYGDFQGAAQCTGDLSPSECQDCLSESIQRLKTECGASTWGE 222
VLA L S G +R I G +G AQCTGDLS ECQDC+SE+I RLK +CG + +G+
Sbjct: 423 VLAGLLGSSGPFR---IGGSGGVRGMAQCTGDLSGGECQDCVSEAISRLKGDCGTADYGD 253
Query: 223 MYLAKCYARYSEGGDHSRHSDDDSNHN 249
M+L KC+ARYS GG H H+
Sbjct: 252 MFLGKCFARYSVGGAHDSSKAHGKAHS 172
Score = 43.5 bits (101), Expect = 4e-05
Identities = 20/57 (35%), Positives = 29/57 (50%)
Frame = -2
Query: 72 PATAGSGDTLYGLYQCRGDLNNDQCYRCVARAVSQLGTLCSAVRGGALQLEGCFVKY 128
P G + G+ QC GDL+ +C CV+ A+S+L C G + L CF +Y
Sbjct: 393 PFRIGGSGGVRGMAQCTGDLSGGECQDCVSEAISRLKGDCGTADYGDMFLGKCFARY 223
>TC14519
Length = 1095
Score = 74.7 bits (182), Expect = 2e-14
Identities = 64/216 (29%), Positives = 93/216 (42%), Gaps = 16/216 (7%)
Frame = +2
Query: 33 CSQPKNTPVSDPVYETGVNSLLTSLVNSASFTNYNNFTVPATAGSGDT-LYGLYQCRGDL 91
CS ++ DP YET +N+LL+ L N + V A+ G G YGL CRGDL
Sbjct: 116 CSMYEHYSSDDP-YETNLNALLSHLKNETPNAGF----VAASKGEGQNQTYGLALCRGDL 280
Query: 92 NNDQCYRCVARAVSQLGTLCSAVRGGALQLEGCFVKYD---NVKFLGVEDKTMVVKKCG- 147
+ C C+A+A+ + C + + E C V Y N + ++D + K
Sbjct: 281 ASTDCGSCIAQAIHDMFEDCPYNKDVMIWHEDCTVGYSKEHNNSGIKIDDTMLCFKSQND 460
Query: 148 -----PSIGLTS-----DALTRRDAVLAYLQTSDGVYRTWRISAYGDFQGAAQCTGDLSP 197
P + + +T+++ + + S V +I Y G AQC DLS
Sbjct: 461 VEVPNPKVFIKKTQEFLSQITQKEELGSNNYASGEV----KIDEYNMVYGYAQCNRDLSS 628
Query: 198 SECQDCLSESIQRLKTECGASTWG-EMYLAKCYARY 232
EC DCL S+ K ECG G +Y C RY
Sbjct: 629 KECSDCLERSLDYAK-ECGRGKEGVRVYSGICRLRY 733
>AW720134
Length = 515
Score = 71.6 bits (174), Expect = 1e-13
Identities = 48/148 (32%), Positives = 71/148 (47%)
Frame = +3
Query: 9 FILTLSAITTPSTSSSIDTFIFGGCSQPKNTPVSDPVYETGVNSLLTSLVNSASFTNYNN 68
F T A TP +++ CS NT ++ Y + +LL SL ++A T ++N
Sbjct: 6 FFFTTEAQDTP-------VYLYKNCST--NTTTTNSAYHLHLRTLLNSLSSNA--TEFHN 152
Query: 69 FTVPATAGSGDTLYGLYQCRGDLNNDQCYRCVARAVSQLGTLCSAVRGGALQLEGCFVKY 128
TV T+ S DT YGL+ CRGD++ C++CV A +L T CS + + C V+Y
Sbjct: 153 STVAGTSPS-DTAYGLFMCRGDVDPAICHQCVVNATHRLQTECSLSNQAIIWYDECTVRY 329
Query: 129 DNVKFLGVEDKTMVVKKCGPSIGLTSDA 156
N F D P +GL + A
Sbjct: 330 SNRSFFSTVDTR-------PRLGLLNTA 392
Score = 43.5 bits (101), Expect = 4e-05
Identities = 26/82 (31%), Positives = 40/82 (48%)
Frame = +3
Query: 152 LTSDALTRRDAVLAYLQTSDGVYRTWRISAYGDFQGAAQCTGDLSPSECQDCLSESIQRL 211
L+S+A ++ +A SD +AYG F C GD+ P+ C C+ + RL
Sbjct: 123 LSSNATEFHNSTVAGTSPSD--------TAYGLFM----CRGDVDPAICHQCVVNATHRL 266
Query: 212 KTECGASTWGEMYLAKCYARYS 233
+TEC S ++ +C RYS
Sbjct: 267 QTECSLSNQAIIWYDECTVRYS 332
>TC19031 similar to GB|AAR24233.1|38566686|BT010866 At1g04520 {Arabidopsis
thaliana;} , partial (30%)
Length = 637
Score = 57.4 bits (137), Expect = 3e-09
Identities = 41/125 (32%), Positives = 66/125 (52%), Gaps = 5/125 (4%)
Frame = +3
Query: 2 VLLLRLLFILTLSAITTP--STSSSIDTFIFGGCSQPKNTPVSDP--VYETGVNSLLTSL 57
+ L LF+LT + ++++ + ++ GCS K+T SDP VY +++L SL
Sbjct: 282 LFLSLFLFLLTNLGLLPQLAESAAAYTSLVYKGCS--KDT-FSDPNGVYSQALSALFGSL 452
Query: 58 VNSASFTNYNNFTVPATAGSGDT-LYGLYQCRGDLNNDQCYRCVARAVSQLGTLCSAVRG 116
V+ ++ + AT GSG + + GL+QCRGDL+N CY CV++ LC
Sbjct: 453 VSQSTKGKF----FKATTGSGQSSITGLFQCRGDLSNSDCYNCVSKLPVLSDKLCGKTVA 620
Query: 117 GALQL 121
+QL
Sbjct: 621 ARVQL 635
Score = 33.5 bits (75), Expect = 0.043
Identities = 18/56 (32%), Positives = 26/56 (46%)
Frame = +3
Query: 151 GLTSDALTRRDAVLAYLQTSDGVYRTWRISAYGDFQGAAQCTGDLSPSECQDCLSE 206
G+ S AL+ L T ++ S G QC GDLS S+C +C+S+
Sbjct: 411 GVYSQALSALFGSLVSQSTKGKFFKATTGSGQSSITGLFQCRGDLSNSDCYNCVSK 578
>AV767962
Length = 582
Score = 54.3 bits (129), Expect = 2e-08
Identities = 37/141 (26%), Positives = 63/141 (44%), Gaps = 3/141 (2%)
Frame = +1
Query: 1 MVLLLRLLF--ILTLSAITTPSTSSSIDTFIFGGCSQPKNTPVSDP-VYETGVNSLLTSL 57
M LL +++F +L +S + S + F C++ NT +S ++ LL
Sbjct: 28 MHLLYKIIFSLLLCISIAESQDLSGPLGEF----CNE--NTKISSGGKLSANIDKLLAEF 189
Query: 58 VNSASFTNYNNFTVPATAGSGDTLYGLYQCRGDLNNDQCYRCVARAVSQLGTLCSAVRGG 117
+ T F + + D +YGL QCRGD++ C C+ A Q+ C
Sbjct: 190 ILKTPSTG---FVATSYGNNQDQVYGLAQCRGDVSTQDCSNCIQDAAKQIRQKCPNQVDA 360
Query: 118 ALQLEGCFVKYDNVKFLGVED 138
+ + CF++Y+N F+G D
Sbjct: 361 RIWYDYCFLRYNNKSFIGDVD 423
Score = 41.6 bits (96), Expect = 2e-04
Identities = 29/105 (27%), Positives = 51/105 (47%), Gaps = 15/105 (14%)
Frame = +1
Query: 180 SAYGDFQ----GAAQCTGDLSPSECQDCLSESIQRLKTECGASTWGEMYLAKCYARYSEG 235
++YG+ Q G AQC GD+S +C +C+ ++ ++++ +C ++ C+ RY+
Sbjct: 223 TSYGNNQDQVYGLAQCRGDVSTQDCSNCIQDAAKQIRQKCPNQVDARIWYDYCFLRYN-- 396
Query: 236 GDHSRHSDDDS----------NHNDDE-IEKTLAILIGLIAGVAL 269
+ S D D+ N D E K L L+G I G A+
Sbjct: 397 -NKSFIGDVDTSFGIFYFNVENVTDPEGFNKELGSLMGKIRGQAV 528
>AV766715
Length = 468
Score = 44.7 bits (104), Expect = 2e-05
Identities = 28/95 (29%), Positives = 41/95 (42%), Gaps = 1/95 (1%)
Frame = -1
Query: 41 VSDP-VYETGVNSLLTSLVNSASFTNYNNFTVPATAGSGDTLYGLYQCRGDLNNDQCYRC 99
VSDP + LL+ L AS T LYGL QC DL++ C +C
Sbjct: 360 VSDPETFNYKTKGLLSELAGKASSNPKLYATGEMELEKSVKLYGLTQCTRDLSSGDCKKC 181
Query: 100 VARAVSQLGTLCSAVRGGALQLEGCFVKYDNVKFL 134
+ A+++L C GG + C +Y+ F+
Sbjct: 180 LDDAINELPNCCDGKEGGRVVGGSCNFRYEIYPFV 76
Score = 38.9 bits (89), Expect = 0.001
Identities = 17/46 (36%), Positives = 22/46 (46%)
Frame = -1
Query: 187 GAAQCTGDLSPSECQDCLSESIQRLKTECGASTWGEMYLAKCYARY 232
G QCT DLS +C+ CL ++I L C G + C RY
Sbjct: 231 GLTQCTRDLSSGDCKKCLDDAINELPNCCDGKEGGRVVGGSCNFRY 94
>BP081081
Length = 498
Score = 31.2 bits (69), Expect = 0.21
Identities = 14/46 (30%), Positives = 23/46 (49%)
Frame = +3
Query: 82 YGLYQCRGDLNNDQCYRCVARAVSQLGTLCSAVRGGALQLEGCFVK 127
+ + QCR L+ C C A ++ CSA G + +GCF++
Sbjct: 321 FTMLQCRNYLSRKDCLACFDAADKKIRN-CSAANGARVIYDGCFLR 455
>TC14463 similar to UP|Q9SLF1 (Q9SLF1) Nodulin-like protein
(At2g16660/T24I21.7), partial (40%)
Length = 1122
Score = 28.5 bits (62), Expect = 1.4
Identities = 27/79 (34%), Positives = 31/79 (39%), Gaps = 16/79 (20%)
Frame = -2
Query: 12 TLSAITTP-----STSSSIDTFIFGGCSQPKNTPVSDPV---------YETG--VNSLLT 55
T +A+ TP S SSS T GG S+ KNTP E G N
Sbjct: 773 TATALQTPK*LASSLSSSAVTVFAGGTSRRKNTPAMQTANGIRASIRRKEAGSSANRAEQ 594
Query: 56 SLVNSASFTNYNNFTVPAT 74
LVN A N F +P T
Sbjct: 593 RLVNIAVLNPTNPFRIPET 537
>TC19523
Length = 524
Score = 28.1 bits (61), Expect = 1.8
Identities = 16/30 (53%), Positives = 17/30 (56%)
Frame = +2
Query: 105 SQLGTLCSAVRGGALQLEGCFVKYDNVKFL 134
SQLG LCS V G L L+ FV KFL
Sbjct: 278 SQLGPLCSLVERGWLPLDEGFVMVKVYKFL 367
>BP072459
Length = 466
Score = 27.3 bits (59), Expect = 3.1
Identities = 15/38 (39%), Positives = 21/38 (54%)
Frame = +3
Query: 8 LFILTLSAITTPSTSSSIDTFIFGGCSQPKNTPVSDPV 45
L +++ S +TP TSSS T I PKN+P P+
Sbjct: 123 LLLISFSISSTPPTSSSSCTTINIQTIGPKNSPYGTPL 236
>BI417786
Length = 513
Score = 26.6 bits (57), Expect = 5.3
Identities = 10/22 (45%), Positives = 13/22 (58%)
Frame = -2
Query: 231 RYSEGGDHSRHSDDDSNHNDDE 252
R +GG H DDD + +DDE
Sbjct: 176 RVRDGGGHDDDDDDDDDDDDDE 111
>BP081173
Length = 396
Score = 26.6 bits (57), Expect = 5.3
Identities = 12/32 (37%), Positives = 18/32 (55%)
Frame = -2
Query: 245 DSNHNDDEIEKTLAILIGLIAGVALIIVFLSF 276
+SNH DE L L+G G+ L+I+ +F
Sbjct: 206 ESNHGGDESAVVLHALVGTATGLLLLILLGNF 111
>TC10241
Length = 641
Score = 26.6 bits (57), Expect = 5.3
Identities = 19/74 (25%), Positives = 32/74 (42%)
Frame = -2
Query: 53 LLTSLVNSASFTNYNNFTVPATAGSGDTLYGLYQCRGDLNNDQCYRCVARAVSQLGTLCS 112
+L +N+ YN T+ G G L+ L + + + +RCV + S L +
Sbjct: 463 ILKQYLNNKKIQLYNTVTIQCKKGEGFVLHALTESPMEDGGSELWRCVFGS-SPLTLAGA 287
Query: 113 AVRGGALQLEGCFV 126
+ +GG GC V
Sbjct: 286 SPKGG-----GCVV 260
>BP043877
Length = 526
Score = 26.6 bits (57), Expect = 5.3
Identities = 11/27 (40%), Positives = 15/27 (54%)
Frame = -1
Query: 181 AYGDFQGAAQCTGDLSPSECQDCLSES 207
A +FQG+ C ++ S C CLS S
Sbjct: 508 AIENFQGSRACVPSVTVSHCTSCLSTS 428
>TC12225
Length = 552
Score = 26.2 bits (56), Expect = 6.9
Identities = 15/50 (30%), Positives = 23/50 (46%), Gaps = 1/50 (2%)
Frame = +3
Query: 184 DFQGAAQCTGDLSPSECQDCLSESI-QRLKTECGASTWGEMYLAKCYARY 232
DF + +G+ E L + Q+ ++C A+TW E KC RY
Sbjct: 6 DFVPSNWSSGEEEDKEMSKSLRHNASQKADSDCIAATWEEEEKTKCCLRY 155
>TC9025 similar to PIR|F86282|F86282 protein F10B6.30 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(55%)
Length = 991
Score = 25.8 bits (55), Expect = 9.0
Identities = 15/50 (30%), Positives = 25/50 (50%)
Frame = +1
Query: 53 LLTSLVNSASFTNYNNFTVPATAGSGDTLYGLYQCRGDLNNDQCYRCVAR 102
+L + ++A+F+N + PAT GD + C L D CY ++R
Sbjct: 43 ILCLIFSAAAFSNAVD-EAPATGTDGDADFIRTSCNTTLYPDVCYTSLSR 189
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.135 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,393,607
Number of Sequences: 28460
Number of extensions: 74354
Number of successful extensions: 371
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 353
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 366
length of query: 292
length of database: 4,897,600
effective HSP length: 90
effective length of query: 202
effective length of database: 2,336,200
effective search space: 471912400
effective search space used: 471912400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0097b.12