BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0093b.6
(443 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CB827145 197 8e-92
NP817340 galactomannan galactosyltransferase [Lotus japonicus] 327 2e-90
TC17581 similar to GB|AAP81802.1|32441254|BT009684 At1g74380 {Ar... 295 3e-86
BP080327 154 3e-38
TC11965 similar to UP|Q9LZJ3 (Q9LZJ3) Alpha galactosyltransferas... 45 2e-05
TC19138 weakly similar to UP|Q9LZJ3 (Q9LZJ3) Alpha galactosyltra... 42 2e-04
BP046735 29 1.8
TC14342 weakly similar to UP|Q8LEJ6 (Q8LEJ6) Arabinogalactan pro... 27 5.2
TC15250 homologue to UP|Q9SWW6 (Q9SWW6) Cellulose synthase catal... 27 5.2
BP067132 27 6.8
BP055695 27 8.8
>CB827145
Length = 496
Score = 197 bits (502), Expect(2) = 8e-92
Identities = 93/95 (97%), Positives = 94/95 (98%)
Frame = +2
Query: 253 VNTGSFLFRNCQWSLDLLDAWAPMGPKGPVREEAGKVLTANLKGRPAFEADDQSALIYLL 312
+NTGSFLFRNCQWSLDLLDAWAPMG KGPVREEAGKVLTANLKGRPAFEADDQSALIYLL
Sbjct: 212 LNTGSFLFRNCQWSLDLLDAWAPMGSKGPVREEAGKVLTANLKGRPAFEADDQSALIYLL 391
Query: 313 LSKKDKWMDKTFLENSFYLHGYWAGLVDRYEEMIE 347
LSKKDKWMDKTFLENSFYLHGYWAGLVDRYEEMIE
Sbjct: 392 LSKKDKWMDKTFLENSFYLHGYWAGLVDRYEEMIE 496
Score = 156 bits (395), Expect(2) = 8e-92
Identities = 71/71 (100%), Positives = 71/71 (100%)
Frame = +1
Query: 182 LDVELAGYWAKLPMIRRLMLSHPEVEWIWWMDSDAFFTDMVFELPLSKYDDYNLVLHGYP 241
LDVELAGYWAKLPMIRRLMLSHPEVEWIWWMDSDAFFTDMVFELPLSKYDDYNLVLHGYP
Sbjct: 1 LDVELAGYWAKLPMIRRLMLSHPEVEWIWWMDSDAFFTDMVFELPLSKYDDYNLVLHGYP 180
Query: 242 DLLFEQKSWIA 252
DLLFEQKSWIA
Sbjct: 181 DLLFEQKSWIA 213
>NP817340 galactomannan galactosyltransferase [Lotus japonicus]
Length = 1314
Score = 327 bits (839), Expect = 2e-90
Identities = 163/401 (40%), Positives = 238/401 (58%), Gaps = 42/401 (10%)
Frame = +1
Query: 67 NVIEETNRILAEIRSDADPSDPDDAAAAE---------TFFS-PNATFTLGPKITGWDLQ 116
N + N + A+++S P + ++A+ TF+ P ++T+ +T WD +
Sbjct: 127 NTDPKLNSVAAKLKSLNLPRNQITTSSAQDLLYDSPETTFYDDPEMSYTMDKPVTNWDEK 306
Query: 117 RKAWLDQNPEYPNFVRGKA-RILLLTGSPPKPCDNPIGDHYLLKSIKNKIDYCRLHGIEI 175
R+ WL +P+F G + RILL+TGS PK C NPIGDH LL+ KNK+DYCR+H I+I
Sbjct: 307 RRQWL---LHHPSFAAGASDRILLVTGSQPKRCHNPIGDHLLLRFFKNKVDYCRIHDIDI 477
Query: 176 VYNLAHLDVELAGYWAKLPMIRRLMLSHPEVEWIWWMDSDAFFTDMVFELPLSKYDDYNL 235
+YN A L ++ YWAK P+++ M++HPEVEWIWW+DSDA TDM F+LPL++Y+++NL
Sbjct: 478 IYNNALLHPKMNSYWAKYPVVKAAMIAHPEVEWIWWVDSDAVITDMEFKLPLNRYNEFNL 657
Query: 236 VLHGYPDLLFEQKSWIAVNTGSFLFRNCQWSLDLLDAWAPMGPKGPVREEAGKVLTANLK 295
++HG+ DL+ ++ SW +N G FL RNCQWSLD +D WA MGP P ++ G+ L A K
Sbjct: 658 IIHGWEDLVKKKHSWTGLNAGVFLMRNCQWSLDFMDVWAAMGPSSPDYKKWGEKLMATFK 837
Query: 296 GRPAFEADDQSALIYLLLSKKDKWMDKTFLENSFYLHGYWAGLV---------------- 339
+ ++DDQ+AL YL+ +DKW +K +LE +Y GYW L
Sbjct: 838 DKVIPDSDDQTALAYLIAMGEDKWTEKIYLEKDYYFEGYWVELAKMYENVSVRYDEVERR 1017
Query: 340 -------------DRYEEMIEKYHPGLGDERWPFVTHFVGCKPCGSYGD--YPVEKCLSS 384
+RY EM E++ G R PF+THF GC+PC + + Y + C +
Sbjct: 1018VGGLRRRHAEKVSERYGEMREEHVKYFGQWRRPFITHFTGCQPCNGHHNPAYAADDCWNG 1197
Query: 385 MERAFNFADNQVLKLYGFRHRGLLSPKIKRIRNETVTPLEF 425
M+RA NFADNQVL+ YG+ R L ++ VTP+ +
Sbjct: 1198MDRALNFADNQVLRTYGYVRRSL--------NDKAVTPIPY 1296
>TC17581 similar to GB|AAP81802.1|32441254|BT009684 At1g74380 {Arabidopsis
thaliana;}, partial (35%)
Length = 804
Score = 295 bits (756), Expect(2) = 3e-86
Identities = 136/142 (95%), Positives = 141/142 (98%)
Frame = +3
Query: 288 KVLTANLKGRPAFEADDQSALIYLLLSKKDKWMDKTFLENSFYLHGYWAGLVDRYEEMIE 347
K+LTANLKGRPAFEADDQSALIYLLLSKK+KWMDK FLENS+YLHGYWAGLVDRYEEM+E
Sbjct: 66 KILTANLKGRPAFEADDQSALIYLLLSKKEKWMDKVFLENSYYLHGYWAGLVDRYEEMLE 245
Query: 348 KYHPGLGDERWPFVTHFVGCKPCGSYGDYPVEKCLSSMERAFNFADNQVLKLYGFRHRGL 407
KYHPGLGDERWPFVTHFVGCKPCGSYGDYPVE+CLSSMERAFNFADNQVLKLYGFRHRGL
Sbjct: 246 KYHPGLGDERWPFVTHFVGCKPCGSYGDYPVERCLSSMERAFNFADNQVLKLYGFRHRGL 425
Query: 408 LSPKIKRIRNETVTPLEFVDQF 429
LSPKIKRIRNETVTPLEFVDQF
Sbjct: 426 LSPKIKRIRNETVTPLEFVDQF 491
Score = 40.4 bits (93), Expect(2) = 3e-86
Identities = 15/20 (75%), Positives = 18/20 (90%)
Frame = +1
Query: 269 LLDAWAPMGPKGPVREEAGK 288
+ D WAPMGPKGP+REEAG+
Sbjct: 10 ICDDWAPMGPKGPIREEAGR 69
>BP080327
Length = 372
Score = 154 bits (389), Expect = 3e-38
Identities = 74/74 (100%), Positives = 74/74 (100%)
Frame = -3
Query: 370 CGSYGDYPVEKCLSSMERAFNFADNQVLKLYGFRHRGLLSPKIKRIRNETVTPLEFVDQF 429
CGSYGDYPVEKCLSSMERAFNFADNQVLKLYGFRHRGLLSPKIKRIRNETVTPLEFVDQF
Sbjct: 370 CGSYGDYPVEKCLSSMERAFNFADNQVLKLYGFRHRGLLSPKIKRIRNETVTPLEFVDQF 191
Query: 430 DIRRHSSESRGSKS 443
DIRRHSSESRGSKS
Sbjct: 190 DIRRHSSESRGSKS 149
>TC11965 similar to UP|Q9LZJ3 (Q9LZJ3) Alpha galactosyltransferase-like
protein (AT3g62720/F26K9_150), partial (11%)
Length = 620
Score = 45.4 bits (106), Expect = 2e-05
Identities = 17/38 (44%), Positives = 28/38 (72%)
Frame = +1
Query: 394 NQVLKLYGFRHRGLLSPKIKRIRNETVTPLEFVDQFDI 431
NQ+L++YGF H+ L S ++KR+RNE+ PL+ D+ +
Sbjct: 1 NQILQMYGFTHKSLASRRVKRVRNESSNPLDVKDELGL 114
>TC19138 weakly similar to UP|Q9LZJ3 (Q9LZJ3) Alpha
galactosyltransferase-like protein
(AT3g62720/F26K9_150), partial (12%)
Length = 458
Score = 42.4 bits (98), Expect = 2e-04
Identities = 26/78 (33%), Positives = 44/78 (56%), Gaps = 5/78 (6%)
Frame = +2
Query: 20 GGRRGRQIQKTFNNVKITILCGFVTILVLRGTIGV---NLSSSDADAVNQNVIE--ETNR 74
G RR RQ+Q+ +T LC F+T+++LRGTIG D D + ++ E +R
Sbjct: 230 GSRRLRQLQRASRRGFLTFLCLFLTVIMLRGTIGAGKFGTPEQDLDEIRHHLGRRVEPHR 409
Query: 75 ILAEIRSDADPSDPDDAA 92
+L E++ + +DP++ A
Sbjct: 410 VLEEVQ--PENNDPNNYA 457
>BP046735
Length = 535
Score = 28.9 bits (63), Expect = 1.8
Identities = 16/37 (43%), Positives = 20/37 (53%)
Frame = +1
Query: 128 PNFVRGKARILLLTGSPPKPCDNPIGDHYLLKSIKNK 164
P F R K RI + GSPP+ N G H +IKN+
Sbjct: 382 PRFTRQKPRIRQVRGSPPEKNRNRTGHH----AIKNR 480
>TC14342 weakly similar to UP|Q8LEJ6 (Q8LEJ6) Arabinogalactan protein-like,
partial (27%)
Length = 724
Score = 27.3 bits (59), Expect = 5.2
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = -2
Query: 78 EIRSDADPSDPDDAAAAETFFS 99
E+ +DA DPD + A TFFS
Sbjct: 291 EVSTDASDDDPDGESEARTFFS 226
>TC15250 homologue to UP|Q9SWW6 (Q9SWW6) Cellulose synthase catalytic
subunit (AT5g17420/T10B6_80), partial (9%)
Length = 602
Score = 27.3 bits (59), Expect = 5.2
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = -1
Query: 296 GRPAFEADDQSALIYLLLSKKDKWMD 321
G+PAF A D + L LS KDKW++
Sbjct: 302 GKPAFSAVDST*LGVRSLSLKDKWIN 225
>BP067132
Length = 499
Score = 26.9 bits (58), Expect = 6.8
Identities = 14/28 (50%), Positives = 16/28 (57%)
Frame = +1
Query: 118 KAWLDQNPEYPNFVRGKARILLLTGSPP 145
K WL E+ FV GK R+LL T PP
Sbjct: 355 KLWLVGWWEFAVFVMGKDRLLLATVGPP 438
>BP055695
Length = 204
Score = 26.6 bits (57), Expect = 8.8
Identities = 13/29 (44%), Positives = 19/29 (64%)
Frame = -3
Query: 274 APMGPKGPVREEAGKVLTANLKGRPAFEA 302
AP+ KG V + +G++LTA G P FE+
Sbjct: 169 APLITKGSVSQLSGQLLTAYGYGAPEFES 83
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.139 0.438
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,563,608
Number of Sequences: 28460
Number of extensions: 131142
Number of successful extensions: 543
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 538
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 540
length of query: 443
length of database: 4,897,600
effective HSP length: 93
effective length of query: 350
effective length of database: 2,250,820
effective search space: 787787000
effective search space used: 787787000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0093b.6